American Journal of Animal and Veterinary Sciences Original Research Articles Clinio-Pathological Changes in Goats Challenged with Corynebacterium Peudotuberculosis and its Exotoxin (PLD) 1Mahmood, Z.K.H., 1F.F. Jesse, 1A.A. Saharee, 2S. Jasni, 1R. Yusoff and 1H. Wahid 1Department of Veterinary Clinical Studies, Faculty of Veterinary Medicine, UPM, Serdang, Selangor, Malaysia 2 Fakulti Perubatan Veterinar, UMK, Kelantan, Malaysia Article history Abstract: Caseous lymphadenitis (CLA) is a chronic disease caused by Received: 03-03-2015 Corynebacterium pseudotuberculosis . However, there is a paucity of data Revised: 15-04-2015 about the C. pseudotuberculosis exotoxin, phospholipase D (PLD) response Accepted: 11-05-2015 during the course of CLA. Therefore, this study was conducted to observe Corresponding Author: the clinical signs and the cellular changes after an experimental infection of Faez Firdaus Jesse the C. pseudotuberculosis and phospholipase D challenge. Twenty six Department of Veterinary crossbred Boer goats aged 12-14 months were divided into 3 groups; the Clinical Studies, Faculty of first group n=6 was inoculated with 1ml sterile PBS s.c. as the control. The Veterinary Medicine, UPM, second group n=10 was inoculated with live C. pseudotuberculosis 1×10 9 Serdang, Selangor, Malaysia cfu s.c. The third group n=10 was i.v. inoculated with PLD 1mL/20 kg, Email:
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[email protected] BW. Both the C. pseudotuberculosis and the PLD treated groups showed a significant increase (p<0.05) in body temperature, heart rate, respiratory rate and body score. Pathologically, the C. pseudotuberculosis and the PLD treated groups showed a significant cellular changes (p<0.05) manifested as edema, congestion, infiltration of inflammatory cells mainly lymphocytes and macrophages, hemorrhage, degeneration and necrosis in the visceral organs including the lungs, heart, liver, spleen, kidneys and lymph nodes.