IBIMA Publishing International Journal of Veterinary Medicine: Research & Reports http://www.ibimapublishing.com/journals/IJVMR/ijvmr.html Vol. 2015 (2015), Article ID 473459, 7 pages DOI: 10.5171/2015.473459 Research Article Serological Detection of Caseous Lymphadenitis in Sheep and Goats Using a Commercial ELISA in Grenada, West Indies H. Hariharan 1 , K. P. Tiwari 2 , S. Kumthekar 3 , D. Thomas 4 , C. Hegamin-Younger 5 , B. Edwards 6 and R. N. Sharma 7 1,2,3,6,7 Department of Pathobiology, School of Veterinary Medicine, P.O. Box 7, St. George’s University, St. George, Grenada, West Indies 4 Ministry of Agriculture, Forestry and Fisheries, St. George, Grenada, West Indies 5 Department of Preventive Medicine and Public Health, School of Medicine, St. George’s University, St. George, Grenada, West Indies Correspondence should be addressed to H. Hariharan;
[email protected] Received date: 22 May 2014; Accepted date: 30 August 2014; Published date: 5 October 2015 Academic Editor: Bhupendra Nath Tripathi Copyright © 2015. H. Hariharan, K. P. Tiwari , S. Kumthekar, D. Thomas , C. Hegamin-Younger, B. Edwards and R. N. Sharma. Distributed under Creative Commons CC-BY 4.0 Abstract Caseous lymphadenitis (CLA) in small ruminants has been diagnosed by culture several times in the state of Grenada. In order to understand the prevalence of CLA, serum samples from 541 sheep and 338 goats from Grenada and Carriacou islands were tested for antibodies against the phospholipase antigen of Corynebacterium pseudotuberculosis , the causative agent of caseous lymphadenitis (CLA) using a commercial ELISA kit: “ELITEST CLA”. The percentage of positive samples among sheep was 7.9, and for goats it was 31.3%, with a significant difference between these two species of animals.