bioRxiv preprint doi: https://doi.org/10.1101/580977; this version posted March 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Enhanced autophagic-lysosomal activity and increased BAG3- mediated selective macroautophagy as adaptive response of neuronal cells to chronic oxidative stress Debapriya Chakraborty1, Vanessa Felzen1, Christof Hiebel1, Elisabeth Stürner1, Natarajan Perumal2, Caroline Manicam2, Elisabeth Sehn3, Franz Grus2, Uwe Wolfrum3, Christian Behl1* 1Institute of Pathobiochemistry, University Medical Center Mainz of the Johannes Gutenberg University, 55099 Mainz, Germany 2Experimental and Translational Ophthalmology, University Medical Center Mainz, 55131 Mainz, Germany 3 Institute for Molecular Physiology, Johannes Gutenberg University, 55128 Mainz, Germany E-mail addresses of authors: D. Chakraborty:
[email protected]; V. Felzen:
[email protected]; C. Hiebel:
[email protected]; E. Stürner:
[email protected]; N. Perumal: nperumal@eye- research.org; C. Manicam:
[email protected]; E. Sehn: sehn@uni- mainz.de; F. Grus:
[email protected]; U. Wolfrum:
[email protected]; *Correspondence to Christian Behl, PhD Institute of Pathobiochemistry University Medical Center of the Johannes Gutenberg University Duesbergweg 6, D-55099 Mainz
[email protected] Tel.: +49-6131-39-25890; Fax: +49-6131-39-25792 1 bioRxiv preprint doi: https://doi.org/10.1101/580977; this version posted March 18, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Abstract Oxidative stress and a disturbed cellular protein homeostasis (proteostasis) belong to the most important hallmarks of aging and of neurodegenerative disorders.