Bioactive Potential of Vitis Labrusca L. Grape Juices from the Southern Region of Brazil
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View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Food Chemistry 173 (2015) 527–535 Contents lists available at ScienceDirect Food Chemistry journal homepage: www.elsevier.com/locate/foodchem Bioactive potential of Vitis labrusca L. grape juices from the Southern Region of Brazil: Phenolic and elemental composition and effect on lipid peroxidation in healthy subjects Isabela Maia Toaldo a, Fernanda Alves Cruz b, Tatiana de Lima Alves b, Jefferson Santos de Gois c, ⇑ Daniel L.G. Borges c,d, Heloisa Pamplona Cunha b, Edson Luiz da Silva b, Marilde T. Bordignon-Luiz a, a Departamento de Ciência e Tecnologia de Alimentos, Universidade Federal de Santa Catarina, Admar Gonzaga Rd., 1346, Itacorubi, 88034-001 Florianópolis, SC, Brazil b Departamento de Análises Clínicas, Universidade Federal de Santa Catarina, Delfino Conti St., Trindade, 88040-900 Florianópolis, SC, Brazil c Departamento de Química, Universidade Federal de Santa Catarina, Des. Vitor Lima Av., Trindade, 88040-900 Florianópolis, SC, Brazil d INCT de Energia e Ambiente do CNPq1, Florianópolis-SC, Brazil article info abstract Article history: Grapes are rich in polyphenols with biologically active properties. Although the bioactive potential of Received 10 April 2014 grape constituents are frequently reported, the effects of Brazilian Vitis labrusca L. grape juices ingestion Received in revised form 8 September 2014 have not been demonstrated in humans. This study identified the phenolic and elemental composition of Accepted 20 September 2014 red and white grape juices and the effect of organic and conventional red grape juice consumption on Available online 23 October 2014 lipid peroxidation in healthy individuals. Concentrations of anthocyanins, flavanols and phenolic acids and the in vitro antioxidant activity were significantly higher in the organic juice. The macro-elements Chemical compounds studied in this article: K, Ca, Na and Mg were the most abundant minerals in all juices. The acute consumption of red grape Malvidin-3-O-glucoside (PubChem CID: juices promoted significant decrease of lipid peroxides in serum and TBARS levels in plasma. It is con- 443652) Cyanidin-3-O-glucoside (PubChem CID: cluded that red V. labrusca L. grape juices produced in Southern Brazil showed lipid peroxidation inhibi- 25244576) tion abilities in healthy subjects, regardless of the cultivation system. Cyanidin-3,5-diglucoside (PubChem CID: Ó 2014 Elsevier Ltd. All rights reserved. 441688) Malvidin-3,5-diglucoside (PubChem CID: 441765) Delphinidin-3-O-glucoside (PubChem CID: 443650) Peonidin-3-O-glucoside (PubChem CID: 443654) Trans-resveratrol (PubChem CID: 445154) Catechin (PubChem CID: 9064) Vanillic acid (PubChem CID: 8468) Myricetin (PubChem CID: 5281672) Keywords: Grape Organic juice Phenolic compounds Elemental composition Oxidative stress 1. Introduction eliciting beneficial functions such as antioxidant, anticarcinogenic, cardioprotective, anti-inflammatory and neuroprotective (Bagchi Natural foods of high nutritional quality play an important role et al., 2000; Chira, Suh, Saucier, & Teissèdre, 2008). Grape and in maintaining human health. Grape (Vitis sp.) contains a diversity grape derivatives are a natural source of phenolic compounds of phytochemical polyphenols that act on human physiology, and represent important sources of polyphenols in the human diet. These compounds are responsible for the bioactive properties of ⇑ Corresponding author. Tel.: +55 48 3721 5376; fax: +55 48 3721 9943. wines and grape juices (Chira et al., 2008; Mulero, Pardo, & E-mail address: [email protected] (M.T. Bordignon-Luiz). Zafrilla, 2010). 1 www.cienam.ufba.br. http://dx.doi.org/10.1016/j.foodchem.2014.09.171 0308-8146/Ó 2014 Elsevier Ltd. All rights reserved. 528 I.M. Toaldo et al. / Food Chemistry 173 (2015) 527–535 Grape juice is a non-fermented grape derivative with typical oline-6-sulphonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl characteristics regarding its colour, aroma and flavour. This natural (DPPH) radicals, and the Folin–Ciocalteu reagent were purchased beverage is highly appreciated and consumed worldwide (Rizzon & from Sigma–Aldrich (St. Louis, MO, USA). Trichloroacetic acid Meneguzzo, 2007). The expansion of viticulture and production of (TCA), iron and ammonium sulphates, n-butanol, ferric chloride, derivatives, as well as the health benefits related to grape polyphe- hydrogen peroxide, 2-thio-barbituric acid (TBA), 5,50-dithiobis nols, have led to a growing interest concerning the nutritional (2-nitrobenzoic acid) (DTNB), butylated hydroxytoluene (BHT), quality and bioactive potential of grape juice (Dani et al., 2007). malondialdehyde (MDA) and triphenylphosphine (TPP) were pur- In recent years, consumers are prioritizing a more healthy diet chased from Sigma–Aldrich. All chromatographic solvents (HLPC and there are increasing knowledge and concern regarding food grade) were purchased from Merck (Darmstadt, Germany). Pure, quality, food safety and environmental protection (Cardoso, analytical standards of polyphenols and organic acids (HPLC grade) Tomazini, Stringheta, Ribeiro, & Pinheiro-Sant’Ana, 2011). The need were purchased from Sigma–Aldrich. Distilled and deionized water for cultivating systems with less aggressive impact on the environ- with resistivity of 18.2 MO cm was generated by a Milli-Q Plus sys- ment, aiming for preservation and sustainable development of viti- tem from Millipore (Bedford, USA). For the elemental analysis, con- culture practices, have contributed to the expansion of organic centrated nitric acid 65% (m/m) from Merck was doubly purified by grape culture and production of organic grape juice (Rombaldi, sub-boiling distillation in a quartz still from Kürner Analysentech- Bergamasqui, Lucchetta, Zanuzo, & Silva, 2004). Organic grapes nik (Rosenheim, Germany). The standard multielemental solution and crops must be grown in safe soil and must remain separate ICP III from Perkin-Elmer (Norwalk, USA) and Ca, K, Na and Rh from conventional products. The application of synthetic pesti- stock standard solution supplied by Sigma–Aldrich (Buchs, Swit- cides, bioengineered substances (GMOs), synthetic fertilizers and zerland) were employed. Argon gas with purity of 99.996%, acety- contaminating compounds are not allowed (Mulero et al., 2010). lene and nitrous oxide were purchased from Linde (Blumenau, American varieties of Vitis labrusca L. are widely cultivated in Brazil). North and South America and are a very relevant source of poly- phenols in Western diets. In Brazil, these varieties represent more than 80% of processed grapes, mainly destined for grape juice pro- 2.2. Grape samples and grape juice processing duction. The most commonly cultivated V. labrusca L. grapes are Bordo, Isabel, Concord and Niagara, which account for around Grapes from V. labrusca L. varieties Bordo, Isabel and Niagara 50% of the total grape production (Nixdorf & Hermosín-Gutiérrez, Branca grown in São Marcos, Rio Grande do Sul State (RS), in the 2010; Toaldo et al., 2013). The main phenolic compounds generally South Region of Brazil, and cultivated in the conventional and present in grape juices are anthocyanins, flavan-3-ols, flavonols, organic systems were used for grape juice preparation through phenolic acids and resveratrol (Natividade, Corrêa, Souza, Pereira, an industrial-scale technological process. The varietal grapes were & Lima, 2013; Rizzon & Meneguzzo, 2007). manually harvested at the stage of technical maturity, with soluble The consumption of polyphenol-rich diets is associated with a solids readings between 14 and 20 °Brix, according to OIV (1990). reduced risk of developing chronic diseases such as atherosclerosis, After harvesting, grapes were kept separately at room temperature heart disease, cancer and diabetes. (20 °C) until juice processing within a period of 2 days. Three vari- Epidemiological studies have shown that these compounds etal grape juices were produced without the addition of water, sug- exert protective effect on oxidative damage induced by free radicals ars, flavourings or preservatives, comprising an organic red juice, present in cells and tissues (O’Byrne, Devaraj, Grundy, & Jialal, elaborated with organic Bordo grapes, a conventional red juice, 2002). The action of polyphenols is associated with modulation of prepared with conventional Isabel and Bordo grapes at a juice pro- important physiological parameters such as antioxidant activity, portion of 1:1 (v/v), and a conventional white juice, prepared with vascular and platelet function, blood pressure and the plasma lipid Niagara Branca grapes. profile, as a result of improved resistance towards oxidative stress, The V. labrusca L. grape juice were produced through a continu- inflammation, and endothelial dysfunction (García-Alonso, Ros, ous-flow process and the grape juice samples were collected after Vidal-Guevara, & Periago, 2006; Van Duynhoven et al., 2010). the final stage of processing. The grape varieties were processed The characteristics of V. labrusca L. grape juices are scarcely separately and when necessary the blending of grape juices was reported in literature and information about organic grape and carried out previously to the final pasteurisation process. Initially, organic grape juices is rarely provided. Furthermore, the identifica- grape berries were crushed using an industrial mechanical crusher tion of important factors responsible for the bioactive properties (EDA, São Paulo, Brazil)