Cold-hardy Grape Germplasm Collection, Geneva, NY Acquire Maintain Conserve Characterize Evaluate Distribute Vitis Genetic Diversity Vitis aestivalis 29 Vitis amurensis 19 Developed selections Wild accessions Vitis cinerea 47 from breeders Acquire through exploration Vitis coignetiae 11 Vitis hybrid 929 USA Vitis labrusca 45 Kazakhstan Vitis monticola 2 Vitis mustangensis 1 Vitis palmata 7 Vitis piasezkii 3 Vitis pseudoreticulata 2 Tenturier cultivar Vitis riparia 80 Vitis romaneti 4 Vitis rupestris 36 Vitis spp. 24 Vitis vinifera 84 Vitis mustangensis, Alabama Leaf senescence Vitis tilifolia 1 (new, disjunct population) Vitis thunbergii 1 Davis, CA Geneva, NY Vitis vulpina 16 Elmer Swenson 1913-2004 Mexico cold tender grapes cold hardy grapes Vitis X champinii 4 pioneering grape breeder ~ 3000 accessions ~ 1371 accessions Vitis X doaniana 2 who revolutionized grape ~ half are Vitis On own roots Vitis X novae-angliae 1 growing in cold and short- Vitis yenshanensis 2 seasoned regions vinifera ~ 68% are Vitis hybrid Ampelopsis aconitifolia var glabra 1 Ampelopsis brevipedunculata 4 Vitis sylvestris - 45 species ~20 species Cissus sp. 1 Total 1371 Research and Collaborations PI 157518 Characterize and Evaluate Functional Marker Phylogenomic Characterizations I1_01_005_PI_588118_01 Molecular: Development Analysis of Grapes DESCRIPTOR No. of accessions SSR fingerprint of every vine • Collaboration Berry length 1026 • Horizon x Ill547-1 • Resequencing Berry shape 980 • Numerous disease response/ • 27 EST-derived markers Berry size 975 viticultural traits • 11,400 aligned base pairs • 304 accessions (North America, Berry weight 979 • Germplasm characterization China, Europe) Berry width 1026 • Marker assisted selection Collaborative evaluations • Phylogeographic analysis Berry flavor 973 1) Berry resveratrol concentration • Evolutionary history Bloom date 1078 2) Leaf resveratrol content • Assess genetic diversity Distribute • develop tools/markers for Brix 979 3) Crown gall susceptibility • Germplasm conservation/use Budburst date ~1350 4) Genome size in base pairs Annual distribution of grape germplasm 1988-2009 5) Genome size in pico grams Cluster length 1023 5000 In progress 4500 Cluster weight 981 4000 6) Phomopsis resistance (Dr. Wilcox, CU) 3500 Vitis9KSNP Array Cluster width 980 7) Powdery mildes resistance (Dr. Reisch, CU) 3000 2500 Digital images ~1800 8) Genetics of grape-pathogen interactions mean = 1262 Supported (Dr. Cadle-Davidson, GGRU) 2000 Photo documentation annually accessions Flesh color 979 1500 9) Key Secondary Metabolites (Dr. Chen, CU) No 1000 DAD1 A, Sig=280,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) DAD1 C, Sig=365,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) DAD1 E, Sig=525,20 Ref=off (C:\DOCUME~1\LC89\DESKTOP\05230008.D) mAU mAU mAU

60

100 20 Peduncle length 980 50

80 500 40 15

60 30 10

40 20 5 Plus Passport Data 0

20 10 Seedlesness 951 0 0 0 88 89 90 91 92 93 94 95 96 97 98 99 00 01 02 03 04 05 06 07 08 09 0 10 20 30 40 50 min 0 10 20 30 40 50 min 0 10 20 30 40 50 min Sex of flower 1245 280nm 365nm 525nm All information available at: Grape total accessions distributed = 27,766 Grape total orders = 1413 10) Virus Indexing and Therapy (Dr. Fuchs, http://www.ars-grin.gov Grape orders per year mean = 64 range 14 - 145 Skin color 1031 CU) Dormant Cuttings, Seeds, Pollen, Flowers, Fruit, Leaves