NTICANCER ESEARCH InternationalA Journal of Cancer ResearchR and Treatment ISSN: 0250-7005

Volume 24, Number 4, July-August 2004

Contents Experimental Studies

Inhibition of the Human Apurinic/Apyrimidinic Endonuclease (Ape1) Repair Activity and Sensitization of Breast Cancer Cells to DNA Alkylating Agents with Lucanthone. M. LUO, M.R. KELLEY (Indianapolis, Indiana, USA)...... 2127

Androgen Withdrawal Inhibits Tumor Growth and is Associated with Decrease in Angiogenesis and VEGF Expression in Androgen-Independent CWR22Rv1 Human Prostate Cancer Model. L. CHENG, S. ZHANG, C.J. SWEENEY, C. KAO, T.A GARDNER, J.N. EBLE (Indianapolis, Indiana, USA) ...... 2135

In Vitro Generation of Cytolytic T Cells Against Human Melanoma Cells Overexpressing HDM2. A. SORURI, A. FAYYAZI, S. GANGL, C. GRIESINGER, C.A. ALBRECHT, T. SCHLOTT (Goettingen; Langen, Germany) ...... 2141

Artemisinin: An Alternative Treatment for Oral Squamous Cell Carcinoma. E. YAMACHIKA, T. HABTE, D. ODA (Seattle, WA, USA; Okayama City, Japan)...... 2153

S19-mRNA Expression in Squamous Cell Carcinomas of the Upper Aerodigestive Tract. V. SENGPIEL, T. ROST, T. GÖRÖGH, I.O. RATHCKE, J.A. WERNER (Marburg; Kiel, Germany) ...... 2161

Three-dimensional Culture System as a Model for Studying Cancer Cell Invasion Capacity and Anticancer Drug Sensitivity. C.J. DOILLON, E. GAGNON, R. PARADIS, M. KOUTSILIERIS (Quebec, Canada; Athens, Greece)...... 2169

Targeting Chemotherapy-induced VEGF Up-regulation by VEGF Antisense Oligonucleotides in HNSCC Cell Lines. F. RIEDEL, K. GÖTTE, U. GOESSLER, H. SADICK, K. HÖRMANN (Mannheim, Germany) ...... 2179 Contents continued on the back cover Contents continued

Cyclin D1 Expression in Endometrioid-type Endometrial Adenocarcinoma is Correlated with Histological Grade and Proliferative Activity, but not with Prognosis. Y. NISHIMURA, J. WATANABE, T. JOBO, N. KATO, T. FUJISAWA, Y. KAMATA, H. KURAMOTO (Kanagawa, Japan) ...... 2185

Low Renal Toxicity of Lipoplatin Compared to Cisplatin in Animals. P. DEVARAJAN, R. TARABISHI, J. MISHRA, Q. MA, A. KOURVETARIS, M. VOUGIOUKA, T. BOULIKAS (Cincinnati, OH; Mountain View, VA, USA; Athens, Greece)...... 2193

TZT-1027 Elucidates Antitumor Activity through Direct Cytotoxicity and Selective Blockade of Blood Supply. N. HASHIGUCHI, T. KUBOTA, J.-I. KOH, Y. YAMADA, Y. SAIKAWA, Y. OTANI, M. WATANABE, K. KUMAI, M. KITAJIMA, J.-I. WATANABE, M. KOBAYASHI (Tokyo; Saitama; Kanagawa, Japan)...... 2201

Probucol, a Hypocholesterolemic Agent, Prevents the Development of Uterine Adenomyosis Induced by Pituitary Grafting in Mice. Y.-F. ZHOU, T. MORI, H. NAGASAWA, T. NAKAYAMA, T. KUBOTA, S. SAKAMOTO (Beijing, China; Chiba; Kanagawa; Tokyo, Japan) ...... 2209

Growth-inhibitory Effects of the Ketone Body, Monoacetoacetin, on Human Gastric Cancer Cells with Succinyl-CoA: 3-Oxoacid CoA-Transferase (SCOT) Deficiency. M. SAWAI, M. YASHIRO, Y. NISHIGUCHI, M. OHIRA, K. HIRAKAWA (Osaka, Japan)...... 2213

Clinical Relevance of HER-2/neu Expression in Germ-cell Testicular Tumors. L. MÁNDOKY, L. GÉCZI, I. BODROGI, J. TÓTH, O. CSUKA, M. KÁSLER, M. BAK (Budapest, Hungary) ...... 2219

Antineoplastic Effect of Immunostimulatory DNA (CpG-ODN) in a Murine C57-BL6 / MB-49 Transitional Cell Carcinoma Model. A. HEGELE, A. DALPKE, P. BARTH, Z. VARGA, K. HEEG, R. HOFMANN, P. OLBERT (Marburg, Germany)...... 2225

Effective Knock Down of Very High ABCG2 Expression by a Hammerhead Ribozyme. P. KOWALSKI, K.M. FARLEY, H. LAGE, E. SCHNEIDER (Albany, NY, USA; Berlin, Germany) ...... 2231

Diffuse Growth Pattern Affects E-Cadherin Expression in Invasive Breast Cancer. U. BRINCK, S. JACOBS, M. NEUSS, K. TORY, W. RATH, B. KULLE, L. FÜZESI (Göttingen; Aachen, Germany) ...... 2237 * Involvement of Endothelial Monocyte Activating Polypeptide II in Tumor Necrosis Factor-alpha-based Anti-cancer Therapy. T.E. LANS, R. VAN HORSSEN, A.M.M. EGGERMONT, T.L.M. TEN HAGEN (Rotterdam, The Netherlands)...... 2243

Chemoimmunotherapy of Murine Mammary Adenocarcinomas. I.H. AMES (Syracuse, NY, USA)...... 2249

Perivascular Mast Cells in Advanced Gastric Adenocarcinomas: An Electron Microscopic Study. R.A. CARUSO, A. IENI, V. FABIANO, G. BASILE, C. INFERRERA (Messina, Italy)...... 2257

Pre-clinical Immunogenicity and Anti-tumour Efficacy of a Deleted Recombinant Human Papillomavirus Type 16 E7 Protein. S. HALLEZ, J.-M. BRULET, C. VANDOOREN, F. MAUDOUX, S. THOMAS, M. HEINDERICKX, A. BOLLEN, R. WATTIEZ, A. JACQUET (Gosselies; Mons, Belgium)...... 2265

Artemisinin Induces Apoptosis in Human Cancer Cells. N.P. SINGH, H.C. LAI (Seattle, WA, USA)...... 2277

Effects of the Polyacetylene Capillin on Human Tumour Cell Lines. L.C. WHELAN, M.F. RYAN (Dublin, Ireland)...... 2281

Involvement of Pattern Recognition Receptors in the Induction of Cytokines and Reactive Oxygen Intermediates Production by Human Monocytes/Macrophages Stimulated with Tumour Cells. B. MYTAR, M. WOLOSZYN, A. MACURA-BIEGUN, B. HAJTO, I. RUGGIERO, B. PIEKARSKA, M. ZEMBALA (Cracow; Poland)...... 2287

Isolated Limb Perfusion Based Anti-p21ras Gene Therapy in a Rat Rhabdomyosarcoma. B. VAN ETTEN, S.T. VAN TIEL, G. AMBAGTSHEER, A.M.M. EGGERMONT, T.L.M. TEN HAGEN (Rotterdam, The Netherlands)...... 2295 Contents continued on the preceding page Contents continued

Acoustic Energy: A New Transfection Method for Cancer of the Prostate, Cancer of the Bladder and Benign Kidney Cells. M.S. MICHEL, P. ERBEN, L. TROJAN, A. SCHAAF, K. KIKNAVELIDZE, T. KNOLL, P. ALKEN (Mannheim, Germany) ...... 2303

Epstein-Barr Virus LMP1 Status in Relation to Apoptosis, P53 Expression and Leucocyte Infiltration in Nasopharyngeal Carcinoma. J.-Y. SHAO, I. ERNBERG, P. BIBERFELD, T. HEIDEN, Y.-X. ZENG, L.- F. HU (Stockholm, Sweden; Guangzhou, China)...... 2309

Can Recurrence of Meningiomas be Predicted? J.-P. KALALA, D. BENOIT, L. DE RIDDER (Ghent, Belgium) ...... 2319

Erythroid Differentiation in K562 Chronic Myelogenous Cells Induced by Crambescidin 800, a Pentacyclic Guanidine Alkaloid. S. AOKI, D. KONG, K. MATSUI, M. KOBAYASHI (Osaka, Japan)...... 2325

Somatostatin Controls LFA-1 Gene Expression by Altering Neuraminidase Expression in Spleen Cells. W.- K. YOON, H.-J. KIM, H.-Y. SON, K.-S. JEONG, S.-J. PARK, T.-H. KIM, S.-H. KIM, S.-R. KIM, S.-Y. RYU (Daejon; Daegu; Kwangju, South Korea) ...... 2331

Amifostine Protects Against Chemotherapy-induced Neurotoxicity: An In Vitro Investigation. C.C.P. VERSTAPPEN, T.J. POSTMA, A.A. GELDOF, J.J. HEIMANS (Amsterdam, The Netherlands) ...... 2337

Antitumour Effectiveness of Hyperthermia is Potentiated by Local Application of Electric Pulses to LPB Tumours in Mice. K.B. KARNER, H. LESNICAR, M. CEMAZAR, G. SERSA (Ljubljana, Slovenia)...... 2343

Elevated Cyclooxygenase-2 Expression Correlates with Distant Metastases in Breast Cancer. G.S. RANGER, V. THOMAS, A. JEWELL, K. MOKBEL (London, UK) ...... 2349

Changes in Serum Soluble VEGFR-1 and Tie-2 Receptors in Colorectal Cancer Patients Following Surgical Resections. K.-F. CHIN, J. GREENMAN, P. REUSCH, E. GARDINER, D. MARME, J. MONSON (Hull, UK; Freiburg, Germany) ...... 2353

Clinical Studies

Correlation of FDG-PET Interpretation with Survival in a Cohort of Glioma Patients. F.S. PARDO, H.J. ARONEN, M. FITZEK, D.N. KENNEDY, J. EFIRD, B.R. ROSEN, A.J. FISCHMAN (Boston, MA; La Jolla; Palo Alto, CA; Helsinki, Finland; Berlin, Germany)...... 2359

Survival in Small Cell Lung Cancer is Independent of Tumor Expression of VEGF and COX-2. J.E. DOWELL, R.H. AMIRKHAN, W.S. LAI, W.H. FRAWLEY, J.D. MINNA (Dallas, TX, USA) ...... 2367

Spectrum of Cytokeratin-positive Cells in the Bone Marrows of Colorectal Carcinoma Patients. J.D. SHETYE, M.L. LILJEFORS, S.O. EMDIN, J.-E. FRÖDIN, K. STRIGÅRD, H.T. MELLSTEDT, A. PORWIT (Stockholm; Umeå, Sweden)...... 2375

Case Report: A Young Woman with Advanced Esophageal Cancer Showing Pathological Complete Response to Neoadjuvant Chemotherapy (CDDP, 5-FU and ADM). Y. NARITAKA, K. OGAWA, T. SHIMAKAWA, Y. WAGATSUMA, K. HAMAGUCHI, S. KONNO, T. KATSv UBE, H. YAGAWA, M. AIBA, H. IDE (Tokyo, Japan)...... 2385

LEA-135 Expression: Its Association with a Lower Risk of Recurrence and Increased Overall Survival of Patients with Lymph Node-positive Primary Invasive Breast Cancer. B. SAHA, N. ZHANG, W.Y. NARITOKU, D.D. TSAO-WEI, S.L. GROSHEN, G. CARLSSON, L. LARSSON, B. GUSTAVSSON, B. CHAIWUN, C.R. TAYLOR, S.A. IMAM (Pasadena; Los Angeles, CA; Göteborg, Sweden; Chiangmai, Thailand)...... 2391

Evaluation of the Clinical Significance of HER2 Amplification by Chromogenic In Situ Hybridisation in Patients with Primary Breast Cancer. N. DANDACHI, O. DIETZE, C. HAUSER-KRONBERGER (Salzburg; Graz, Austria)...... 2401 Contents continued on the preceding page Contents continued

The Expression and Prognostic Value of ·-, ‚- and Á-Catenins in Renal Cell Carcinoma. S. AALTOMAA, P. LIPPONEN, V. KÄRJÄ, S. LUNDSTEDT, J. LAPPI, V.-M. KOSMA (Kuopio; Jyväskylä; Tampere, Finland)...... 2407

Absence of BRAF Gene Mutations Differentiates Spitz Nevi from Malignant Melanoma. D. MIHIC-PROBST, A. PERREN, S. SCHMID, P. SAREMASLANI, P. KOMMINOTH, P.U. HEITZ (Baden, Switzerland)...... 2415

Radiofrequency Ablation Combined with Percutaneous Ethanol Injection in the Treatment of Hepatocellular Carcinoma and Portal Vein Neoplastic Thrombosis. G. POGGI, C. GATTI, C. TERAGNI, ANGELO DELMONTE, GIOVANNI BERNARDO (Pavia, Italy)...... 2419

Long-term Intravenous Administration of Activated Autologous Lymphocytes for Cancer Patients does not Induce Antinuclear Antibody and Rheumatoid Factor. T. YAMAGUCHI, K. BAMBA, A. KITAYAMA, Y. KUROIWA, K. YOSHIMATSU, T. SHIMAKAWA, K. OGAWA, T. SEKINE, N. SHIMIZU, K. YAMAMOTO (Tokyo, Japan)...... 2423

Genetic Polymorphisms of Hormone-related Genes and Prostate Cancer Risk in the Japanese Population. T. FUKATSU, Y. HIROKAWA, T. ARAKI, T. HIOKI, T. MURATA, H. SUZUKI, T. ICHIKAWA, H. TSUKINO, D. QIU, T. KATOH, Y. SUGIMURA, R. YATANI, T. SHIRAISHI, M. WATANABE (Tsu; Matsusaka; Suzuka; Chiba; Miyazaki; Yokohama, Japan)...... 2431

Lymph Node Dissection in Papillary or Follicular Thyroid Carcinoma. M. BONONI, A. TOCCHI, V. CANGEMI, A. VECCHIONE, M.R. GIOVAGNOLI, A. DE CESARE, E. FIORI, P. VOLPINO, S. BROZZETTI, M. MEUCCI, S. SCOZZAFAVA, A. CAVALLARO (Rome, Italy)...... 2439 * Boosting the Immune Response: An Alternative Combination Therapy for Cancer Patients. G. THYPHRONITIS, M. KOUTSILIERIS (Athens, Greece) ...... 2443

Thymidylate Synthetase (TS) Genotype and TS/dihydropyrimidine Dehydrogenase mRNA Level as an Indicator in Determining Chemosensitivity to 5-Fluorouracil in Advanced Gastric Carcinoma. F. TORIUMI, T. KUBOTA, Y. SAIKAWA, M. YOSHIDA, Y. OTANI, M. WATANABE, K. KUMAI, M. KITAJIMA (Tokyo, Japan) ...... 2455

Randomized Phase II Study Comparing Mitomycin, Cisplatin Plus Doxifluridine with Cisplatin Plus Doxifluridine in Advanced Unresectable Gastric Cancer. W. KOIZUMI, Y. FUKUYAMA, T. FUKUDA, T. AKIYA, K. HASEGAWA, Y. KOJIMA, N. OHNO, M. KURIHARA (Kanagawa; Chiba; Gunma; Fukushima, Tokyo Japan) ...... 2465

Association of K-ras Mutations with Liver Metastases from Colorectal Carcinoma. A. TSUNODA, T. IIJIMA, Y. TSUNODA, K. NAKAO, M. MIYAKI, M. KUSANO (Tokyo, Japan) ...... 2471

Detection of Aberrant Methylation as a Tumor Marker in Serum of Patients with Gastric Cancer. D. ICHIKAWA, H. KOIKE, H. IKOMA, D. IKOMA, N. TANI, E. OTSUJI, K. KITAMURA, H. YAMAGISHI (Kyoto, Japan) ...... 2477

Efficacy and Toxicities of Concurrent Chemoradiation for Elderly Patients with Esophageal Cancer. T. UNO, K. ISOBE, H. KAWAKAMI, N. UENO, H. KOBAYASHI, H. SHIMADA, H. MASTUBARA, S. OKAZUMI, Y. NABEYA, T. SHIRATORI, T. OCHIAI, T. KAWATA, H. ITO (Chiba, Japan) ...... 2483

Tumor Shrinkage Evaluation During and After Preoperative Doxorubicin and Cyclophosphamide Followed by Docetaxel in Patients with Breast Cancer. F. PUGLISI, M. MANSUTTI, G. APRILE, A.M. MINISINI, C. DI LORETO, M. BAZZOCCHI, V. LONDERO, C. CEDOLINI, G. GENTILE, S. PIZZOLITTO, A. PIGA, A. SOBRERO (Udine; Genoa, Italy) ...... 2487

Expression of Dihydropyrimidine Dehydrogenase in Cancer Cells but not in Stromal Cells Predicts the Efficacy of Fluorouracil Treatment in Patients with Gastric Carcinoma. K. HISAMITSU, S. TSUJITANI, K. YAMAGUCHI, K. FUKUDA, I. KONISHI, N. KAIBARA (Yonago, Japan)...... 2495

Contents continued on the preceding page Contents continued

Oncogenes Modulate Cell Sensitivity to Apoptosis Induced by Glucose Deprivation. M. KANSARA, M.V. BERRIDGE (South Wellington, New Zealand)...... 2503

Serum Levels of S-100B Protein and Neuron-specific Enolase in Glioma Patients: A Pilot Study. M.J. VOS, T.J. POSTMA, F. MARTENS, B.M.J. UITDEHAAG, M.A. BLANKENSTEIN, W.P. VANDERTOP, B.J. SLOTMAN, J.J. HEIMANS (Amsterdam, The Netherlands)...... 2511

Pancreatic Polypeptide is Increased in Patients with Advanced Malignant Disease. A. HJALMARSEN, R.M. BREMNES, U. AASEBØ, R. JORDE (Tromsø, Norway) ...... 2515 * Prognostic and Predictive Molecular Markers in Colorectal Carcinoma. R. BENDARDAF, H. LAMLUM, S. PYRHÖNEN (Turku, Finland) ...... 2519

Usefulness of 99mTc-pertechnetate Scintigraphy and Fine-needle Aspiration Cytology in Patients with Solitary Thyroid Nodules and Thyroid Cancer. F. LUMACHI, L. VAROTTO, S. BORSATO, A. TREGNAGHI, P. ZUCCHETTA, M.C. MARZOLA, D. CECCHIN, F. BUI (Padova, Italy)...... 2531

Interphase FISH for Follow-up of Philadelphia Chromosome-positive Chronic Myeloid Leukemia Treatment. N. DOUET-GUILBERT, F. MOREL, T. LE CHARPENTIER, M.-J. LE BRIS, A. HERRY, P. MORICE, P. BOURQUARD, J.F. ABGRALL, C. BERTHOU, M. DE BRAEKELEER (Brest, France) ...... 2535

Differences in Prognosis of Colorectal Cancer Patients Based on the Expression of Sialyl Lewisa, Sialyl Lewisx and Sialyl Tn Antigens in Serum and Tumor Tissue. S. AKAMINE, T. NAKAGOE, T. SAWAI, T. TSUJI, K. TANAKA, S. HIDAKA, S.-I. SHIBASAKI, A. NANASHIMA, H.YAMAGUCHI, T. NAGAYASU, T. YASUTAKE (Nagasaki, Japan) ...... 2541

Lack of Association Between RNASEL Arg462Gln Variant and the Risk of Breast Cancer. A. SEVINç, D. YANNOUKAKOS, I. KONSTANTOPOULOU, E. MANGUOGLU, G. LÜLECI, T. çOLAK, C. AKYERLI, G. çOLAKOGLU, M. TEZ, I. SAYEK, V. GERASSIMOS, G. NASIOULAS, E. PAPADOPOULOU, L. FLORENTIN, E. KONTOGIANNI, B. BOZKURT, N.A. KOCABAS, A.E. KARAKAYA, I.G. YULUG, T. ÖZçELIK (Ankara; Istanbul, Turkey; Athens, Greece) ...... 2547

Ethnic Differences in Diet and Associations with Clinical Markers of Prostate Disease in New Zealand Men. A. HARRIS, M.A. GRAY, D.P. SLANEY, M.L.TURLEY, J.R. FOWLES, P. WEINSTEIN (Wellington, New Zealand; Perth, WA, Australia)...... 2551

Failure of Alkylating Agents to Improve Induction Chemotherapy of Oropharyngeal Squamous Cell Cancer. L. OLASZ, Z. NYÁRÁDY, Á. NÉMETH, L. KIRÁLYFALVI, I. EMBER (Pécs, Hungary) ...... 2557

Bone Metastasis from Glioblastoma Multiforme without Central Nervous System Relapse: A Case Report. A. FABI, A. VIDIRI, C. CARAPELLA, A. PACE, E. OCCHIPINTI, F. CAROLI, A. MIRRI, P. CARLINI, F. COGNETTI (Rome, Italy)...... 2563

A Phase II Trial of Weekly Paclitaxel and Gemcitabine in Non-small Cell Lung Cancer Patients Previously Treated with Platinum and Vinorelbine. V. DONGIOVANNI, A. ADDEO, A. BERRUTI, L. BUFFONI, D. DONGIOVANNI, M.A. POLIMENI, C. FISSORE, C. BARONE, O. BERTETTO (Torino; Orbassano, Italy) ...... 2567

Serum Level of Cathepsin B and its Density in Men with Prostate Cancer as Novel Markers of Disease Progression. H. MIYAKE, I. HARA, H. ETO (Akashi; Kobe, Japan) ...... 2573

Predictive Effect of p53 and p21 Alteration on Chemotherapy Response and Survival in Locally Advanced Adenocarcinoma of the Esophagus. P.A.M. HEEREN, F.W.H. KLOPPENBERG, H. HOLLEMA, N.H. MULDER, R.E. NAP, J.TH.M. PLUKKER (Groningen,The Netherlands)...... 2579

* Review (pages 2243, 2443, 2519)