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Observation of the Yeasts

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OBSERVATION OF THE YEASTS Supervizor: Tomáš Náhlík Students: Jana Drtilová Helena Procházková Academic and University Center of Nové Hrady THIS PROJECT HAS RECEIVED FINANCIAL SUPPORT FROM THE EUROPEAN SOCIAL FUND AND FROM GOVERNMENT OF THE CZECH REPUBLIC. AIMS Our goals Preparation of samples of Micro- yeasts for light photography microscopy Observing Observing of yeasts cell cycle yeasts organells 1/46 -Schizosaccharomyces pombe- SchizosaccharomycesScientific classificationpombe -Scientific classification- LATIN ENGLISCH REGNUM KINGDOM Fungi PHYLUM PHYLUM Ascomycota, Basidiomycota DIVISIO DIVISION Taphrinomycotina CLASSIS CLASS Schizosaccharomycetes ORDO ORDER Schizosaccharomycetales FAMILIA FAMILY Schizosaccharomycetaceae GENUS GENUS Schizosaccharomyces SPECIES SPECIES S. Pombe BINOMIAL NAME Schizosaccharomyces pombe 2/46 FUNDAMENTAL INFORMATION What are Yeasts are yeasts? eukaryotic Did not form micro- fruiting organisms Antony von Leeuwenhoek, 17th century 3/46 FUNDAMENTAL INFORMATION Occurence Skin and Caused intestinal tract deceases Earth, air Foodstuffs 5/46 FUNDAMENTAL INFORMATION Uses In foodstuffs In medicine In agriculture In industry 5/46 SPECIES OF THE YEASTS 1 2 Saccharomyces cerevisiae [1] Yarrowia lipolytica [2] 4 3 Yeast [3] Schizosaccharomyces pombe 6/46 SCHIZOSACCHAROMYCES POMBE Isolation: • Lindner 1893 • East African millet beer Size: • 3 – 4 μm in diameter • 7 – 14 μm in length [4] 7/46 PREPARATION OF SPECIMENS 8/46 TYPES OF SPECIMENS Between glasses Vaseline chamber Very fast preparation ADVANTAGE Live longer (1 – 2 days) The organisms died very Complicated preparation DISADVANTAGE quickly (1 - 2 hours) Thicker specimen 9/46 CELL CYCLE S G2 M G1 [5] 10/46 CELL CYCLE Elongation Forming of cell wall Dividing of cells 11/46 OUR EXPERIMENT • Sample preparation • Observation and photography • Evaluation of results 12/46 COLONY GROWHT Number of Cells 12 10 8 6 4 Number of Cells Number of Cells Number 2 0 0 25 158 166 252 259 263 369 399 Time [min] 13/46 Oth generation S. Pombe 0 14/46 15/46 Forming of cell wall 16/46 Dividing of cells 17/46 1st generation S. Pombe 1 1 18/46 Elongation 19/46 Elongation 20/46 Elongation 21/46 22/46 Forming of cell wall Forming of cell wall 23/46 24/46 Dividing of cells 25/46 2nd generation S. Pombe 2 2 2 2 26/46 Elongation 27/46 Forming of cell wall 28/46 Dividing of cells 29/46 3rd generation S. Pombe 3 3 3 3 3 3 3 3 30/46 Elongation 31/46 Elongation 32/46 Elongation 33/46 Elongation 34/46 Forming of cell wall 35/46 Elongation 36/46 Forming of cell wall 37/46 38/46 4th generation S. Pombe 4 4 4 4 39/46 GENEALOGY OF YEASTS D, hh:mm:ss [Image Nr.] D, hh:mm:ss [Image Nr.] 40/46 CONCLUSIONS AimsAims / / Preparation of samples Microphotography Cell cycle Oscilation of organells [6] 41/46 42/46 ACKNOWLEDGEMENTS • Ing. Štěpánka Kučerová – for cultivation of cultures • Mgr. Tomáš Náhlík – for valuable advice and project management • And all people who prepare the project Schola Ludus and Summer academical course 43/46 [7] THANK YOU FOR YOUR ATTENTION! [8] 44/46 PICTURES • [1] http://files.myopera.com/MCOB/albums/380322/thumbs/S._cer_lugo2l.jpg_thumb.jpg • [2] http://www.biomed.cas.cz/~sychrova/img-staff2/yarrowia-lipolytica.jpg • [3] http://www.cukrar.cz/pictures/a-X3KWNR8YE6-small.jpg • [4]http://upload.wikimedia.org/wikipedia/commons/a/ac/Millet_beer_in_Rhumsiki.jpg • [5]http://www-bcf.usc.edu/~forsburg/images/pombe5.jpg • [6]University of Wien • [7]http://www.umbr.cas.cz/res/biofyzika/LK-NH.htm • [8]http://www.funny-foto.cz/pictures.php?cathegory=zvirata&strana=22 45/46 LITERATURE • Přispěvatelé Wikipedie, Kvasinky [online], Wikipedie: Otevřená encyklopedie, c2010, Datum poslední revize 7. 07. 2010, 20:28 UTC, [citováno 26. 07. 2010] http://cs.wikipedia.org/w/index.php?title=Kvasinky&oldid=5548940 • Wikipedia contributors. "Yeast." Wikipedia, The Free Encyclopedia. Wikipedia, The Free Encyclopedia, 23 Jul. 2010. Web. 26 Jul. 2010 • Taxonomic rank. (2010, July 25). In Wikipedia, The Free Encyclopedia. Retrieved 12:18, July 26, 2010, from http://en.wikipedia.org/w/index.php?title=Taxonomic_rank&oldid=375428710 • The story behind a loaf of bread, http://www.botham.co.uk/bread/yeast.htm [citováno 26.07.2010] 46/46.
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