DOCSLIB.ORG

Oral Fibropapillomatosis and Epidermal Hyperplasia of the Lip In

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Oral Fibropapillomatosis and Epidermal Hyperplasia of the Lip In www.nature.com/scientificreports OPEN Oral fbropapillomatosis and epidermal hyperplasia of the lip in newborn lambs associated with Received: 14 December 2017 Accepted: 21 August 2018 bovine Deltapapillomavirus Published: xx xx xxxx Sante Roperto1, Valeria Russo1, Federica Corrado2, Francesca De Falco1, John S. Munday3 & Franco Roperto4 Congenital fbropapillomatosis of the gingiva and oral mucosa and epidermal hyperplasia of the lip are described, for the frst time, in two newborn lambs. Expression of the E5 oncoprotein of bovine deltapapillomavirus types 2 (BPV-2) and -13 (BPV-13) was detected in both fbropapillomas and the hyperplastic epidermal cells suggesting the BPV infection was the cause of the proliferative lesions. No DNA sequences of BPV-1 and BPV-14 were detected. Both BPV-2 and BPV-13 DNA were also amplifed from peripheral blood mononuclear cells (PBMCs) of the newborn lambs’ dams. The concordance between BPV genotypes detected in the blood of dam and the oral and skin pathological samples of their ofspring suggests that a vertical hematogeneous transmission was most likely source of BPV infection. Immunoblotting revealed the presence of E5 dimers allowing the viral protein to be biologically active. E5 dimers bind and activate the platelet derived growth factor β receptor (PDGFβR), a major molecular mechanism contributing to disease. The detection of E5 protein within the proliferating cells therefore adds further evidence that the BPV infection was the cause of the proliferative lesions seen in these lambs. This is the frst evidence of vertical transmission of BPVs in sheep resulting in a clinical disease. Papillomaviruses (PVs) are small, non-enveloped, double-stranded DNA viruses that infect mucosal and cutane- ous epithelia in most animal species, and in humans1. While there are currently over 200 types of human papillo- maviruses (HPVs) recognized, only 24 bovine papillomaviruses (BPVs) have been fully characterized2. Based on the degree of nucleotide sequence diversity of the L1 gene, BPVs are classified in five genera: Deltapapillomavirus (δPV) (BPV-1, -2, -13, -14), Epsilonpapillomavirus (εPV) (BPV-5, -8), Xipapillomavirus (χPV) (BPV-3, -4, -6, -9, -10, -11, -12, -15, -17, -20, -23, -24), Dyokappapapillomavirus (dyoκPV) (BPV-16, -18, -22), and Dyoxipapillomavirus (dyoχPV) (BPV-7). BPV-19 and BPV-21 are currently unclassifed2–4. Te bovine δPVs have some unique biological properties such as inducing fbropapillomas in their respective hosts5 and being able to cause trans-species transmission1. Te bovine δPVs have been associated with sarcoids in horses6,7, African lions8, and domestic cats9. Furthermore, bovine δPVs have been found in cutaneous lesions of bufaloes10–12, Cape mountain zebras, girafes and sable antelopes13,14. BPV-1 and BPV-2 DNA has also been detected in a squamous cell carcinoma of head and neck in a Connemara mare15, and in a series of cutaneous spindle cell tumors in horses16. Bovine δPVs are also associated with bladder tumors in cattle and bufalo17–20. Very recently, BPV-2 DNA only has been detected from ovine cutaneous wart lesions21. Horizontal route is thought to be the predominant mode of papillomaviral transmission. However, there is a growing interest in vertical PV transmission22 although the clinical relevance of vertical spread remains uncer- tain23. In humans, PV DNA has been detected in amniotic fuid, placenta and umbilical cord blood samples. Tis supports the possibility of in utero PV infection24,25 and vertical infection of PVs has been suggested to occur in up to 20% of people25. However, although PVs appear to be able to be transmitted vertically, such congenital 1Dipartimento di Medicina Veterinaria e delle Produzioni Animali, Università di Napoli Federico II,via Delpino, 1 - 80137, Napoli, Italy. 2Istituto Zooproflattico Sperimentale del Mezzogiorno, via della Salute, 2 - 80055, Portici (Na), Italy. 3Pathobiology, School of Veterinary Sciences, Massey University, Palmerston North, New Zealand. 4Dipartimento di Biologia, Università di Napoli Federico II, 80126, Napoli, Italy. Correspondence and requests for materials should be addressed to S.R. (email: [email protected]) SCIENTIFIC REPORTS | (2018) 8:13310 | DOI:10.1038/s41598-018-31529-9 1 www.nature.com/scientificreports/ Figure 1. Oral fbropapillomatosis of lambs. Orderly proliferation of well-diferentiated keratinocytes causing branched or fused rete ridges and supported by stalks of stromal fbroblasts is shown. infections rarely cause clinically visible lesions in children at birth23. In the non-human species, both abortive and productive infections of BPV-2 have been detected in the placenta of pregnant cows26. In addition, BPV-2- and BPV-13-associated placental papillomatosis was recently reported in bufaloes27. In the present paper two newborn lambs that had congenital fbropapillomatosis of the gingiva and oral mucosa and epidermal hyperplasia of the lip are described. Both lesions were found to contain BPV-2 and BPV- 13 DNA and E5 oncoprotein expression. To the authors’ knowledge, congenital PV-associated lesions have never been previously reported in sheep. Additionally, this is the frst evidence that BPVs may be able to be transmitted vertically, and cause disease, in sheep. Results Clinical and gross pathology examination. Clinical examination of lamb No. 1 revealed a 4 × 5 cm area of roughened, reddish painful proliferative tissue, within the rostral mandibular gingiva, that appeared to have impeded the eruption of the central incisors and caused displacement of the remaining teeth. Te opposing skin of the upper lip of this lamb was thickened and covered by thick scaling crusts (Supplemental Fig. S1). Clinical examination of lamb No. 2 revealed a 0.5 × 1 cm smooth and reddened proliferation within the gingival mucosa extending at both facial and lingual levels from the lower incisors. Te gingival involvement and rapid growth of proliferative tissue resulted in displacement of the incisor teeth (Supplemental Fig. S2). Microscopic and ultrastructural fndings. Te proliferative tissue seen in the mandibular rostral gingiva of lamb No. 1 as well as the proliferation of gingiva and oral mucosa of lamb No. 2 was characterized by a pap- illary or acanthotic growth of well-diferentiated squamous keratinocytes that retained an orderly maturation pattern. Epithelial hyperplasia was supported by marked stromal stalks composed of plump, haphazard fbroblasts with round vesicular nuclei and new vessel-forming endothelial cells. Te hyperplastic epithelium extends as branches or rete pegs into the underlying submucosa (Fig. 1). Numerous mitotic fgures were seen; some of them showed a spindle orientation that was perpendicular and/or oblique to the intact basement membrane (BM) resulting in asymmetric cell divisions (ACDs). Ultrastructurally, the proliferating cells were well-diferentiated keratinocytes, ofen containing compound melanosomes. Most of the keratinocytes showed prominent nucleoli because of enlarged fbrillar centers (Fig. 2). Poorly developed desmosomes, known also as attenuated desmo- somes, being the intermediate electron dense line lacking and causing the cells to lose their cohesiveness, were seen. Furthermore, some desmosomes appeared to have an abnormal size (giant desmosomes) (Supplemental Fig. S3). Intranuclear microtubular inclusions were detected in numerous keratinocytes. Asymmetric mitosis was documented by transmission electron microscope (TEM) showing the formation of two daughter cells of unequal size (Supplemental Fig. S4). Irregular epidermal hyperplasia covered by orthokeratotic hyperkeratosis was seen on examination of the skin from the lip. Te epithelial projections commonly appeared to represent expansion of the follicular infundibulum. Numerous keratinocytes with shrunken nuclei surrounded by a clear cytoplasmic halo (koilocytes) and a high number of symmetric and asymmetric mitotic fgures in basal and suprabasal layers were seen (Fig. 3). ACDs were clearly shown by the formation of micronuclei by TEM (Supplemental Fig. S5). Some attenuated as well as giant desmosomes and desmosomes-like structures were also seen in some keratinocytes of the stratum spinosum which sometimes appeared to have ring-shaped nucleoli. A difuse cell hyperplasia of adnexal epithelium was also SCIENTIFIC REPORTS | (2018) 8:13310 | DOI:10.1038/s41598-018-31529-9 2 www.nature.com/scientificreports/ Figure 2. Oral fbropapillomatosis of lambs. Ultrastructural aspect of well diferentiated keratinocytes containing phagocytic compound melanosomes. Notice marginated and central enlarged nucleolar fbrillar centers. Figure 3. Epidermal hyperplasia of the lip of lambs. Epithelial projections representing expansion of the follicular infundibulum is shown. Several koilocytes (yellow arrows) and numerous symmetric and asymmetric mitoses are shown (black arrows) (inset). present. Infammation characterized by neutrophils, eosinophils, lymphoid cells, and macrophages was seen in the dermis. Virological analysis. Te presence of papillomatous hyperplasia within the lesions and clinical history of the fock suggested a possible role of viral agents in disease development. Hyperplastic lesions of the mouth and skin can develop in the disease contagious ecthyma (also known as scabby mouth and contagious pustular dermatitis) that is caused by the orf parapoxvirus, (OrfV). However, the presence of koilocytosis in the present cases was considered as indication of a papillomavirus infection. PCR screening revealed infection by bovine
Load more

Recommended publications
  • Changes to Virus Taxonomy 2004
    Arch Virol (2005) 150: 189–198 DOI 10.1007/s00705-004-0429-1 Changes to virus taxonomy 2004 M. A. Mayo (ICTV Secretary) Scottish Crop Research Institute, Invergowrie, Dundee, U.K. Received July 30, 2004; accepted September 25, 2004 Published online November 10, 2004 c Springer-Verlag 2004 This note presents a compilation of recent changes to virus taxonomy decided by voting by the ICTV membership following recommendations from the ICTV Executive Committee. The changes are presented in the Table as decisions promoted by the Subcommittees of the EC and are grouped according to the major hosts of the viruses involved. These new taxa will be presented in more detail in the 8th ICTV Report scheduled to be published near the end of 2004 (Fauquet et al., 2004). Fauquet, C.M., Mayo, M.A., Maniloff, J., Desselberger, U., and Ball, L.A. (eds) (2004). Virus Taxonomy, VIIIth Report of the ICTV. Elsevier/Academic Press, London, pp. 1258. Recent changes to virus taxonomy Viruses of vertebrates Family Arenaviridae • Designate Cupixi virus as a species in the genus Arenavirus • Designate Bear Canyon virus as a species in the genus Arenavirus • Designate Allpahuayo virus as a species in the genus Arenavirus Family Birnaviridae • Assign Blotched snakehead virus as an unassigned species in family Birnaviridae Family Circoviridae • Create a new genus (Anellovirus) with Torque teno virus as type species Family Coronaviridae • Recognize a new species Severe acute respiratory syndrome coronavirus in the genus Coro- navirus, family Coronaviridae, order Nidovirales
    [Show full text]
  • Genome Characterization of a Bovine Papillomavirus Type 5 from Cattle in the Amazon Region, Brazil
    Virus Genes DOI 10.1007/s11262-016-1406-y Genome characterization of a bovine papillomavirus type 5 from cattle in the Amazon region, Brazil 1,2 1 1 1 Flavio R. C. da Silva • Cı´ntia Daudt • Samuel P. Cibulski • Matheus N. Weber • 3 3 4 1 Ana Paula M. Varela • Fabiana Q. Mayer • Paulo M. Roehe • Cla´udio W. Canal Received: 11 September 2016 / Accepted: 20 October 2016 Ó Springer Science+Business Media New York 2016 Abstract Papillomaviruses are small and complex viruses Keywords Papillomaviridae Á Epsilonpapillomavirus Á with circular DNA genome that belongs to the Papillo- BPV5 Á Complete genome Á Phylogeny mavirus family, which comprises at least 39 genera. The bovine papillomavirus (BPV) causes an infectious disease that is characterized by chronic and proliferative benign Introduction tumors that affect cattle worldwide. In the present work, the full genome sequence of BPV type 5, an Epsilonpa- Viruses from the Papillomaviridae family infect epithelia pillomavirus, is reported. The genome was recovered from in amniotes and are associated with asymptomatic infec- papillomatous lesions excised from cattle raised in the tions, proliferative benign lesions, and different cancers in Amazon region, Northern Brazil. The genome comprises humans and other animals [1]. Papillomaviruses (PVs) 7836 base pairs and exhibits the archetypal organization of have circular, double-stranded DNA genomes of *8kbin the Papillomaviridae. This is of significance for the study length. The organization of PV genomes consists of the of BPV biology, since currently available full BPV genome early and the late regions and the noncoding region sequences are scarce. The availability of genomic infor- between them.
    [Show full text]
  • Molecular Characterization and Developing a Point-Of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt
    animals Article Molecular Characterization and Developing a Point-of-Need Molecular Test for Diagnosis of Bovine Papillomavirus (BPV) Type 1 in Cattle from Egypt Mohamed El-Tholoth 1,2,3 , Michael G. Mauk 2, Yasser F. Elnaker 4 , Samah M. Mosad 1, Amin Tahoun 5, Mohamed W. El-Sherif 6, Maha S. Lokman 7,8 , Rami B. Kassab 8,9 , Ahmed Abdelsadik 10, Ayman A. Saleh 11 and Ehab Kotb Elmahallawy 12,13,* 1 Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura 35516, Egypt; [email protected] (M.E.-T.); [email protected] (S.M.M.) 2 Department of Mechanical Engineering and Applied Mechanics, University of Pennsylvania, Philadelphia, PA 19104, USA; [email protected] 3 Health Sciences Division, Veterinary Sciences Program, Al Ain Men’s Campus, Higher Colleges of Technology, Al Ain 17155, UAE 4 Department of Animal Medicine (Infectious Diseases), Faculty of Veterinary Medicine, The New Valley University, El-Karga 72511, New Valley, Egypt; [email protected] 5 Department of Animal Medicine, Faculty of Veterinary Medicine, Kafrelshkh University, Kafrelsheikh 33511, Egypt; [email protected] 6 Department of Surgery, Anesthesiology and Radiology, Faculty of Veterinary Medicine, The New Valley University, El-Karga 72511, New Valley, Egypt; [email protected] 7 Biology Department, College of Science and Humanities, Prince Sattam bin Abdul Aziz University, Alkharj 11942, Saudi Arabia; [email protected] 8 Department of Zoology and Entomology, Faculty of Science, Helwan University, 11795 Cairo, Egypt;
    [Show full text]
  • Isoodon Obesulus) in Western Australia
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Elsevier - Publisher Connector Virology 376 (2008) 173–182 Contents lists available at ScienceDirect Virology journal homepage: www.elsevier.com/locate/yviro Genomic characterization of a novel virus found in papillomatous lesions from a southern brown bandicoot (Isoodon obesulus) in Western Australia Mark D. Bennett a,⁎, Lucy Woolford a, Hans Stevens b, Marc Van Ranst b, Timothy Oldfield c, Michael Slaven a, Amanda J. O'Hara a, Kristin S. Warren a, Philip K. Nicholls a a School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Western Australia, 6150, Australia b Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10 B-3000, Leuven, Belgium c Wattle Grove Veterinary Hospital, 791 Welshpool Road, Wattle Grove, Western Australia, 6107, Australia article info abstract Article history: The genome of a novel virus, tentatively named bandicoot papillomatosis carcinomatosis virus type 2 (BPCV2), Received 21 January 2008 obtained from multicentric papillomatous lesions from an adult male southern brown bandicoot (Isoodon Returned to author for revision obesulus) was sequenced in its entirety. BPCV2 had a circular double-stranded DNA genome consisting of 3 March 2008 7277 bp and open reading frames encoding putative L1 and L2 structural proteins and putative large T antigen Accepted 14 March 2008 and small t antigen transforming proteins. These genomic features, intermediate between Papillomaviridae Available online 25 April 2008 and Polyomaviridae are most similar to BPCV1, recently described from papillomas and carcinomas in the endangered western barred bandicoot (Perameles bougainville).
    [Show full text]
  • Evidence to Support Safe Return to Clinical Practice by Oral Health Professionals in Canada During the COVID-19 Pandemic: a Repo
    Evidence to support safe return to clinical practice by oral health professionals in Canada during the COVID-19 pandemic: A report prepared for the Office of the Chief Dental Officer of Canada. November 2020 update This evidence synthesis was prepared for the Office of the Chief Dental Officer, based on a comprehensive review under contract by the following: Paul Allison, Faculty of Dentistry, McGill University Raphael Freitas de Souza, Faculty of Dentistry, McGill University Lilian Aboud, Faculty of Dentistry, McGill University Martin Morris, Library, McGill University November 30th, 2020 1 Contents Page Introduction 3 Project goal and specific objectives 3 Methods used to identify and include relevant literature 4 Report structure 5 Summary of update report 5 Report results a) Which patients are at greater risk of the consequences of COVID-19 and so 7 consideration should be given to delaying elective in-person oral health care? b) What are the signs and symptoms of COVID-19 that oral health professionals 9 should screen for prior to providing in-person health care? c) What evidence exists to support patient scheduling, waiting and other non- treatment management measures for in-person oral health care? 10 d) What evidence exists to support the use of various forms of personal protective equipment (PPE) while providing in-person oral health care? 13 e) What evidence exists to support the decontamination and re-use of PPE? 15 f) What evidence exists concerning the provision of aerosol-generating 16 procedures (AGP) as part of in-person
    [Show full text]
  • Isoodon Obesulus) in Western Australia
    Virology 376 (2008) 173–182 Contents lists available at ScienceDirect Virology journal homepage: www.elsevier.com/locate/yviro Genomic characterization of a novel virus found in papillomatous lesions from a southern brown bandicoot (Isoodon obesulus) in Western Australia Mark D. Bennett a,⁎, Lucy Woolford a, Hans Stevens b, Marc Van Ranst b, Timothy Oldfield c, Michael Slaven a, Amanda J. O'Hara a, Kristin S. Warren a, Philip K. Nicholls a a School of Veterinary and Biomedical Sciences, Murdoch University, Perth, Western Australia, 6150, Australia b Laboratory of Clinical and Epidemiological Virology, Department of Microbiology and Immunology, Rega Institute for Medical Research, University of Leuven, Minderbroedersstraat 10 B-3000, Leuven, Belgium c Wattle Grove Veterinary Hospital, 791 Welshpool Road, Wattle Grove, Western Australia, 6107, Australia article info abstract Article history: The genome of a novel virus, tentatively named bandicoot papillomatosis carcinomatosis virus type 2 (BPCV2), Received 21 January 2008 obtained from multicentric papillomatous lesions from an adult male southern brown bandicoot (Isoodon Returned to author for revision obesulus) was sequenced in its entirety. BPCV2 had a circular double-stranded DNA genome consisting of 3 March 2008 7277 bp and open reading frames encoding putative L1 and L2 structural proteins and putative large T antigen Accepted 14 March 2008 and small t antigen transforming proteins. These genomic features, intermediate between Papillomaviridae Available online 25 April 2008 and Polyomaviridae are most similar to BPCV1, recently described from papillomas and carcinomas in the endangered western barred bandicoot (Perameles bougainville). This study also employed in situ hybridization Keywords: to definitively demonstrate BPCV2 DNA within lesion biopsies.
    [Show full text]
  • Papillomaviruses: a Systematic Review
    Genetics and Molecular Biology, 40, 1, 1-21 (2017) Copyright © 2017, Sociedade Brasileira de Genética. Printed in Brazil DOI: http://dx.doi.org/10.1590/1678-4685-GMB-2016-0128 Review Article Papillomaviruses: a systematic review Rodrigo Pinheiro Araldi1,2, Suely Muro Reis Assaf1, Rodrigo Franco de Carvalho1, Márcio Augusto Caldas Rocha de Carvalho1,3, Jacqueline Mazzuchelli de Souza1,2, Roberta Fiusa Magnelli1,2, Diego Grando Módolo1, Franco Peppino Roperto4, Rita de Cassia Stocco1 and Willy Beçak1 1Laboratório de Genética, Instituto Butantan, São Paulo, SP, Brazil. 2Programa de Pós-graduação Interunidades em Biotecnologia, Instituto de Ciências Biomédicas (ICB), Universidade de São Paulo (USP), São Paulo, SP, Brazil. 3Programa de Aprimoramento Profissional (PAP), Instituto Butantan, São Paulo, SP, Brazil 4Dipartimento di Medicina Veterinaria e Produzioni Animali, Università degli Studi di Napoli Federico II, Napoli, Campania, Italy. Abstract In the last decades, a group of viruses has received great attention due to its relationship with cancer development and its wide distribution throughout the vertebrates: the papillomaviruses. In this article, we aim to review some of the most relevant reports concerning the use of bovines as an experimental model for studies related to papillo- maviruses. Moreover, the obtained data contributes to the development of strategies against the clinical conse- quences of bovine papillomaviruses (BPV) that have led to drastic hazards to the herds. To overcome the problem, the vaccines that we have been developing involve recombinant DNA technology, aiming at prophylactic and thera- peutic procedures. It is important to point out that these strategies can be used as models for innovative procedures against HPV, as this virus is the main causal agent of cervical cancer, the second most fatal cancer in women.
    [Show full text]
  • Phylogenetic Position of an Uncharacterized Brazilian Strain of Bovine Papillomavirus in the Genus Xipapillomavirus Based on Sequencing of the L1 Open Reading Frame
    Genetics and Molecular Biology, 33, 4, 745-749 (2010) Copyright © 2010, Sociedade Brasileira de Genética. Printed in Brazil www.sbg.org.br Short Communication Phylogenetic position of an uncharacterized Brazilian strain of bovine papillomavirus in the genus Xipapillomavirus based on sequencing of the L1 open reading frame Michele Lunardi1, Marlise P. Claus1, Amauri A. Alfieri1, Maria Helena P. Fungaro2 and Alice F. Alfieri1 1Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brazil. 2Laboratório de Genética Molecular, Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, PR, Brazil. Abstract The use of PCR assays with degenerate primers has suggested the existence of numerous as yet uncharacterized bovine papillomaviruses (BPV). Despite the endemic nature of BPV infections, the identification of BPV types in Bra- zilian cattle is still only sporadic. However, in a recent analysis of a partial segment of the L1 gene, we observed nota- ble diversity among the BPV types detected. The aim of this study was to determine the phylogenetic position of the previously identified wild strain BPV/BR-UEL2 detected in the state of Paraná in Brazil. Since previous analysis of the partial L1 sequence had shown that this strain was most closely related to BPV type 4, genus-specific primers were designed. Phylogenetic analysis using complete L1 ORF sequences revealed that BPV/BR-UEL2 was related to BPV types classified in the genus Xipapillomavirus and shared the highest L1 nucleotide sequence similarity with BPV type 4 (78%). This finding suggests that BPV/BR-UEL2 should be classified as a potential new type of BPV in the genus Xipapillomavirus.
    [Show full text]
  • Molecular Identification of Bovine Papillomaviruses in Dairy and Beef Cattle: First Description of Xi- and Epsilonpapillomavirus in Turkey
    Turkish Journal of Veterinary and Animal Sciences Turk J Vet Anim Sci (2016) 40: 757-763 http://journals.tubitak.gov.tr/veterinary/ © TÜBİTAK Research Article doi:10.3906/vet-1512-64 Molecular identification of bovine papillomaviruses in dairy and beef cattle: first description of Xi- and Epsilonpapillomavirus in Turkey 1, 2 3 Veysel Soydal ATASEVEN *, Özgür KANAT , Yaşar ERGÜN 1 Department of Virology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey 2 Department of Pathology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey 3 Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Mustafa Kemal University, Hatay, Turkey Received: 21.12.2015 Accepted/Published Online: 02.05.2016 Final Version: 15.12.2016 Abstract: In the current study, 23 papilloma/tumor-like samples obtained from cattle having clinical lesions and 9 blood samples collected from healthy-appearing cattle in Turkey were examined for bovine papillomavirus (BPV) DNA using the degenerate primers FAP59/64, and the different types of BPV were distinguished by type-specific primer sets. Furthermore, histopathological studies of papillomavirus were performed. A total of 7 BPV types (BPVs 1, 2, 3, 4, 6, 8, 9), including genera Deltapapillomavirus, Xipapillomavirus, and Epsilonpapillomavirus, were identified. In all samples, BPV-1 was the most common genotype (90.6%). Overall, coinfections were determined in 26 of the examined samples (81.3%), and coinfection with BPV-1/BPV-4/BPV-8 (21.9%) was the most frequently identified using BPV type-specific primers. Moreover, bovine leukemia virus, an oncogenic retrovirus, was detected from three cattle with tumor-like lesions (13.0%), which were also coinfected by different BPV types.
    [Show full text]
  • Infectious Diseases of the Horse
    INFECTIOUS DISEASES of the Diagnosis, pathology, HORSE management, and public health J. H. van der Kolk DVM, PhD, dipl ECEIM Department of Equine Sciences–Medicine Faculty of Veterinary Medicine Utrecht University, the Netherlands E. J. B. Veldhuis Kroeze DVM, dipl ECVP Department of Pathobiology Faculty of Veterinary Medicine Utrecht University, the Netherlands MANSON PUBLISHING / THE VETERINARY PRESS Copyright © 2013 Manson Publishing Ltd ISBN: 978-1-84076-165-8 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system or transmitted in any form or by any means without the written permission of the copyright holder or in accordance with the provisions of the Copyright Act 1956 (as amended), or under the terms of any licence permitting limited copying issued by the Copyright Licensing Agency, 33–34 Alfred Place, London WC1E 7DP, UK. Any person who does any unauthorized act in relation to this publication may be liable to criminal prosecution and civil claims for damages. A CIP catalogue record for this book is available from the British Library. For full details of all Manson Publishing Ltd titles please write to: Manson Publishing Ltd, 73 Corringham Road, London NW11 7DL, UK. Tel: +44(0)20 8905 5150 Fax: +44(0)20 8201 9233 Email: [email protected] Website: www.mansonpublishing.com Commissioning editor: Jill Northcott Project manager: Kate Nardoni Copy editor: Ruth Maxwell Layout: DiacriTech, Chennai, India Colour reproduction: Tenon & Polert Colour Scanning Ltd, Hong Kong Printed by: Grafos SA, Barcelona, Spain CONTENTS Introduction 5 Actinomyces spp. 90 Abbreviations 6 Dermatophilus congolensis: ‘rain scald’ or streptotrichosis 92 Chapter 1 Corynebacterium pseudotuberculosis: ‘pigeon fever’ 94 Bacterial diseases 9 Mycobacterium spp.: tuberculosis 97 Nocardia spp.
    [Show full text]
  • Taxonomy Bovine Ephemeral Fever Virus Kotonkan Virus Murrumbidgee
    Taxonomy Bovine ephemeral fever virus Kotonkan virus Murrumbidgee virus Murrumbidgee virus Murrumbidgee virus Ngaingan virus Tibrogargan virus Circovirus-like genome BBC-A Circovirus-like genome CB-A Circovirus-like genome CB-B Circovirus-like genome RW-A Circovirus-like genome RW-B Circovirus-like genome RW-C Circovirus-like genome RW-D Circovirus-like genome RW-E Circovirus-like genome SAR-A Circovirus-like genome SAR-B Dragonfly larvae associated circular virus-1 Dragonfly larvae associated circular virus-10 Dragonfly larvae associated circular virus-2 Dragonfly larvae associated circular virus-3 Dragonfly larvae associated circular virus-4 Dragonfly larvae associated circular virus-5 Dragonfly larvae associated circular virus-6 Dragonfly larvae associated circular virus-7 Dragonfly larvae associated circular virus-8 Dragonfly larvae associated circular virus-9 Marine RNA virus JP-A Marine RNA virus JP-B Marine RNA virus SOG Ostreid herpesvirus 1 Pig stool associated circular ssDNA virus Pig stool associated circular ssDNA virus GER2011 Pithovirus sibericum Porcine associated stool circular virus Porcine stool-associated circular virus 2 Porcine stool-associated circular virus 3 Sclerotinia sclerotiorum hypovirulence associated DNA virus 1 Wallerfield virus AKR (endogenous) murine leukemia virus ARV-138 ARV-176 Abelson murine leukemia virus Acartia tonsa copepod circovirus Adeno-associated virus - 1 Adeno-associated virus - 4 Adeno-associated virus - 6 Adeno-associated virus - 7 Adeno-associated virus - 8 African elephant polyomavirus
    [Show full text]
  • Teat Papillomatosis Associated with Bovine Papillomavirus Types 6, 7, 9, and 10 in Dairy Cattle from Brazil
    Brazilian Journal of Microbiology 44, 3, 905-909 (2013) Copyright © 2013, Sociedade Brasileira de Microbiologia ISSN 1678-4405 www.sbmicrobiologia.org.br Short Communication Teat papillomatosis associated with bovine papillomavirus types 6, 7, 9, and 10 in dairy cattle from Brazil 1,* 1,*,# 1 1 Claudia C. Tozato , Michele Lunardi , Alice F. Alfieri , Rodrigo A.A. Otonel , Giovana W. Di Santis2, Brígida K. de Alcântara1, Selwyn A. Headley2, Amauri A. Alfieri1 1Laboratório de Virologia Animal, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brazil. 2Laboratório de Patologia, Departamento de Medicina Veterinária Preventiva, Universidade Estadual de Londrina, Londrina, PR, Brazil. Submitted: February 7, 2012; Approved: September 10, 2012. Abstract This study describes the clinical, histopathological, and virological characterization of teat papillo- matosis from Brazilian dairy cattle herds. Four types of bovine papillomavirus were identified (BPV6, 7, 9, and 10); one of these (BPV7) is being detected for the first time in Brazilian cattle. Key words: papilloma, BPV, genotyping. Papillomaviruses (PVs) are small (52-55 nm), non- (BPV5 and 8), and a yet unnamed PV genus (BPV7) (Ber- enveloped, double-stranded DNA oncoviruses that repli- nard et al., 2010; Hatama et al., 2011; Zhu et al., 2012; cate in the nucleus of squamous epithelial cells and can in- Lunardi et al., 2013). Moreover, the occurrence of numer- duce warts in the skin and mucosal epithelia of most higher ous additional viral types has been proposed based on par- vertebrate species. Some specific viral types have the po- tial nucleotide sequence analysis of the major capsid pro- tential to cause malignant progression in papillomatous le- tein L1, obtained from both benign cutaneous lesions and sions of animals and humans (Antonsson et al., 2002; de swab samples of healthy skin from cattle (Antonsson and Villiers et al., 2004).
    [Show full text]