Diversity of Macrofungi in Semi-Evergreen and Moist Deciduous Forest of Shimoga District-Karnataka, India
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J Mycol Pl Pathol, Vol. 38, No. 1, 2008 21 Guman Devi Verma Memoria Best Woman Scientist Award Diversity of Macrofungi in Semi-Evergreen and Moist Deciduous Forest of Shimoga District-Karnataka, India Swapna S, Syed Abrar and Krishnappa M* Department of P.G. Studies and Research in Applied Botany, Jnana Sahyadri, Kuvempu University, Shankaraghatta-577451, Shimoga, Karnataka.Email: [email protected] Abstract Shimoga district is one of the richest floristic area, located in between 13º 27' and 14º 39' N lat and 74º 38' and 76º 34' E long with a wide range of ecosystems and species diversity. Studies on the taxonomy and diversity of macrofungi are gaining importance, as many macrofungi are becoming extinct and facing threat of extinction because of habitat destruction. The current study deals with the diversity of macrofungi in semi-evergreen and moist deciduous forest of Shimoga district Karnataka. The average rainfall is 140 cm, temp is 25C and RH is 60 to 100%. The macrofungal diversity is very rich due to litter decomposition. Surveys were conducted from April 2005 to June 2007 in 10 sampling stations, five of their in semi-evergreen and its remaining five in moist deciduous forest. A total of 100 transects (50 each in semi-evergreen and moist deciduous) were studied each measuring 50 x 20 m. The macrofungi were collected, characterized, allotted accession number, preserved and all the collected specimens were photographed. A total of 778 species of macrofungi belonging to 43 families, 101 genera were enumerated of which 242 species were identified to genus level and 73 were identified to species level. Some of the currently identified genera are Agaricus sp., Albatrellus sp., Amanita sp., Arrhenia sp., Boletus sp., Cantharellus sp., Clavaria sp., Cordyceps sp., Delicatula sp., Entoloma sp., Flammulina sp., Hygrocybe sp., Hypholoma sp., Laccaria sp., Lepiota sp., Lycoperdon sp., Macrolepiota sp., Microporus sp., Panellus sp., Phellinus sp., Ramaria sp., Schizophyllum sp., Tricholoma sp., Auricularia auricula, Calocera cornea, Calvatia cyathiformis, Cariolus versicolor, Clavilinopsis laeticolor, Clitocybe rivulosa, Cyathus striatus, Daldinia concentrica, Dictyophora cinnabarina, Geastrum laccata. Geoglossum fallax, Hygrocybe singeri, Marasmius quercophilus, Pleurotus ostreatus, Ramaria botrytis, Scutellina scutellata, Stereum ostrea, Tremella mesenterica and Xylaria polymorpha. Among the collected species Laccaria sp. forms most denser (D=15.36), abundant (Ab=256) and frequency (F=0.06) in semi-evergreen forest and Schizophyllum sp. forms the most denser (D=16.48), abundant (Ab=164.8) and frequency (F=0.1) in moist deciduous forest. The Shannon diversity index and Simpson index were calculated to be 5.57 and 1.12 in semi- evergreen forest, which indicates the very high species richness of the study site. In moist deciduous forest, the Shannon diversity index was 5.42 and the Simpson index was 0.011. Further investigation is in progress. Keywords: Diversity, survey, sporocarps, wood rotting fungi, species richness Citation: Swapna S, Syed Abrar and Krishnappa M. 2008. Diversity of macrofungi in semi-evergreen and moist deciduous forest of Shimoga district-Karnataka, India. J Mycol Pl Pathol 38(1):21-26. The variety and galaxy of The number of existing fungi worldwide has been fungi and their natural estimated to 1.5 million species (Hawksworth 2004). beauty occupy prime place One-thirds of the fungal diversity of the globe exists in in the biological world and India and of this, only 50% are characterized until now India has been a cradle for (Manoharachary et al 2005). The number of fungi such fungi. Defining the recorded in India exceeds 27,000 species, the largest number of fungi on earth biotic community after insects. Fungi are not only has always been a point of beautiful but play a significant role in the daily life of discussion and several human beings besides their utilization in industry, studies have focused on agriculture, medicine (Cowan 2001; Chang and Miles enumerating the world’s 2004), food industry, textiles, bioremediation, natural fungal biodiversity (Crous cycling, in recycling nutrients and decomposing the et al 2006). Only a fraction of total fungal wealth has dead organic matter (Molina et al 1993; Keizer 1998; been subjected to scientific scrutiny and mycologists Pilz 2001) in soil and litter (Hunt 1999; Gates 2005), as continue to unravel the unexplored and hidden wealth. biofertilizers and many other ways. Fungal 22 J Mycol Pl Pathol, Vol. 38, No. 1, 2008 biotechnology has become an integral part of the human district (Fig. 1) that varied in altitude, they include welfare (Manoharachary et al 2005). The presence of Kodachadri (1343 m), Hosanagara (572 m), Mastikatte extensive biodiversity available in tropical forests has (640 m), Hulikal ghat (800 m) and Yedur (800 m) in been identified as the treasure box for the emerging semi-evergreen forest and Shimoga (571 m), field of biotechnology. Macrofungi are defined as fungi Thimlapura (570 m), Umbalebailu (520 m), that form macroscopic fruiting bodies, such as gilled Lekkinkoppa (570 m) and Shankaraghatta (690 m) in fungi, jelly fungi, coral fungi, stinkhorns, bracket fungi, moist deciduous forest. The soil is most fertile due litter puffballs, and bird’s nest fungi (Hawksworth et al 1995; decomposition. Due to high humidity and humus Richards and Murray 2002; Bates 2006). There are accumulation provides the luxuriant growth of micro many thousands of species that all are unique and each and macro-fungal diversity. species beautiful in its own way. It is usual for a Survey. Surveys are particularly sensitive to timing and particular fungus to produce a visible fruiting body only location of observations. Macrofungi exhibit pattern of under a precise combination of conditions, including diversity that are related to largely to substratum and geographic location, elevation, temperature, humidity, host availability (Natarajan et al 2005). Temp regiones light and surrounding flora. Macrofungi grow interact with the habitat of the fungus either stimulate or prolifically and are found in many parts of the world retard fruiting (Lodge et al 2004). Surveying is best just (Smith 1963). Studies on this subject, in fact, have been after the period of rain but some macrofungi can be carried out in different countries (Peck 1873; Stojchev found any time in some locations (Susan metzler and 1995) and new species for the world macrofungal flora Van metzler 1992). Difficulty was small proportions of have been recorded (Kerrigan 1989; Laferriare 1990). macrofungi were visible on a single visit. Hence Macrofungi studies have long been of interest to repeated surveys were done during Apr 2005 to Jun scientists as well as the public due to their important 2007 in all the sampling stations. The sporocarps role in human welfare, in food industry, in medicinally encountered were collected and analyzed for its identity. effective products and in biodegradation (Ozturk et al Density, abundance, frequency (Table 1) and diversity 2003). Macrofungi were considered ideal for the indices like Shannon and Simpson were calculated purpose of evaluation as biosorbents, because it has using standard protocols. been demonstrated that many fungal species exhibit high biosorptive potentials (Muraleedharan et al 1995). Work has been carried out on antimicrobial activities of lower fungi but edible mushrooms have not been adequately explored (Jonathan and Fasidi 2003). Our objective was to benchmark diversity of macrofungi and characterize, collect, preserve, and evaluate edibility and medicinal value of different macrofungal species in semi-evergreen and moist deciduous forest of Shimoga district in Karnataka. Materials and Methods Study area. Shimoga district is a part of naturally rich biodiversity malnad region of Karnataka known as Malenada hebbagilu. It is situated between 13º 27' and 14º 39' N lat and 74º 38' and 76º 34' E long. The geographical area of the district is about 10,58,000 ha with a forest area of 3,27,000 ha. The average rainfall is about 140 cm, temp is avg. of max 30.5C and min of 19.6C and RH 60 to 100%. Vegetation can be classified into tropical wet evergreen forest, tropical semi Figure 1. Study sites of semi-evergreen and moist evergreen forest, moist deciduous forest, dry deciduous deciduous forest of Shimoga district, Karnataka, forest and tropical thorn forest (Champion and Seth India 1968). Some of the floristic elements are Artocarpus spp., Dipterocarpus spp., Mangifera indica, Tectona Sampling. Vegetation zones and plant associations are grandis, Dalbergia spp., Terminalia spp., Mimosops useful criteria to use when dividing a landscape for elangi, Cinnomom spp., Sterculata guttata, Celastris sampling. Many macrofungi occur only in association paniculatus, Gnetum spp., Garcinia gummifera, with particular family or plants. The macrofungi Deospyros spp., Tylophora spp. and Rubia cardifolia.. generally fruit when temp was above 15C and RH more The study was made in the 10 stations of Shimoga than 80-85%. J Mycol Pl Pathol, Vol. 38, No. 1, 2008 23 Table 1. Macrofungi in sem-ievergreen (SE) and moist deciduous (MD) forest of Shimoga, Karnataka, India Density Abundance Frequency Species name Density Abundance Frequency Species name SE MD SE MD SE MD SE MD SE MD SE MD Agaricus sp. 0.57 0.07 3.68 2.92 0.14 0.05 Hydnellum sp. 0.56 0.08 9.33 1.33 0.06 0.06 Agrocybe sp. 0.26 0.12 4.33 3.00 0.06 0.04 Hygrocybe singeri 0.08 0.10 4.00 5.00 0.02 0.02 Albatrellus sp. 0.07 0.06 3.00 3.00 0.03 0.02 Hypholoma sp. 1.08 0.96 54.00 48.00 0.02 0.02 Alboleptonia Inocybe sp. 1.50 0.16 25.00 4.00 0.06 0.04 serricilla 0.16 0.16 4.00 2.67 0.04 0.06 Laccaria sp. 0.17 3.38 4.21 39.62 0.06 0.15 Aleuria sp.