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DNA Barcoding of the Leaf-Mining Moth Subgenus Ectoedemia S. Str
Contributions to Zoology, 81 (1) 1-24 (2012) DNA barcoding of the leaf-mining moth subgenus Ectoedemia s. str. (Lepidoptera: Nepticulidae) with COI and EF1-α: two are better than one in recognising cryptic species Erik J. van Nieukerken1, 2, Camiel Doorenweerd1, Frank R. Stokvis1, Dick S.J. Groenenberg1 1 Netherlands Centre for Biodiversity Naturalis, PO Box 9517, 2300 RA Leiden, The Netherlands 2 E-mail: [email protected] Key words: pairwise difference, Palearctic Abstract Species recognition ..................................................................... 7 The Ectoedemia angulifasciella group ................................... 7 We sequenced 665bp of the Cytochrome C Oxidase I (COI) The Ectoedemia suberis group .............................................. 10 barcoding marker for 257 specimens and 482bp of Elongation The Ectoedemia populella group .......................................... 10 Factor 1-α (EF1-α) for 237 specimens belonging to the leaf- The Ectoedemia subbimaculella group ................................ 11 mining subgenus Ectoedemia (Ectoedemia) in the basal Lepi- Discussion ........................................................................................ 13 dopteran family Nepticulidae. The dataset includes 45 out of 48 One or two genes ...................................................................... 13 West Palearctic Ectoedemia s. str. species and several species Barcoding gap ........................................................................... 15 from Africa, North America and Asia. -
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LCTRONICLCTRONIC CATALOGCATALOG Hanging Basket Cucumbers CU20‐20 ‐ Bush Crop Cucumbers CU79‐20 ‐ Mexican Sour Gherkin Cucumbers 55 days. Cucumis sativus. (F1) This early 75 days. Melothria scabra. Open Pollinated. maturing compact bush plant produces high The plant produces heavy yields of 1 to 2" yields of 6 to 8" green cucumbers. They are long cucumbers that look like miniature crisp, tender, and flavorful. Excellent in watermelons. They have the sweet salads or for making dills or fancy sweet cucumber flavor, followed by a surprising pickles. Bush cucumbers produce very nice sourness, which leads you to believe they straight cucumbers. It grows very short are already pickled! Great for Perfect for vines, only 1 to 2 feet long, and is one of the salads, snacks, and pickling. They fall off best varieties for container gardens and the vines when ripe. This cucumber grows small gardens. An excellent choice for home best if grown on trellis or stakes. The most gardens. Disease Resistant: Ccu. cold‐tolerant of all cucumbers and will continue to produce until the first frost. Perfect for container gardening, hanging baskets, or small gardens where space is very limited!. Also known as the Cucamelon and Mouse Melon. An heirloom from Mexico and Central America. CU98‐10 ‐ Salad Bush Cucumbers CU23‐20 ‐ Spacemaster 80 Cucumbers 1988 All‐America Selections Winner! 60 days. Cucumis sativus. Open Pollinated. Compact bush type plant produces heavy 57 days. Cucumis sativus. (F1) This early yields of 7 to 8" long nicely shaped dark maturing compact bushy plant produces green cucumbers. Great for pickling when good yields of 8” long by 2 ¼” wide glossy small, and slicing when they get bigger. -
Nature of Ergastic Substances in Some Poaceae Seeds
African Journal of Biotechnology Vol. 10(48), pp. 9800-9803, 29 August, 2011 Available online at http://www.academicjournals.org/AJB ISSN 1684–5315 © 2011 Academic Journals Full Length Research Paper Nature of ergastic substances in some Poaceae seeds Idu, M. 1* and Onyibe, H. I. 2 1Department of Botany, University of Benin, PMB 1154, Benin City, Edo State, Nigeria. 2Department of Botany, Ambrose Alli University, PMB. 14, Ekpoma, Edo State, Nigeria. Accepted 6 May, 2011 Seeds of 48 species of Poaceae were examined for the presence of alkaloid, protein, fats and oil, inulin, starch and tannins. All taxa investigated indicated the presence of starch, fats and oils and protein and were devoid of tannins and inulin. Only 12 seed samples indicated the presence of alkaloids. The Positively indicated taxa are suggested for further investigation. Key words: Poaceae, starch, fats and oils, alkaloid, protein, tannins, inulin. INTRODUCTION Ergastic substances are products of metabolism. These reserve (Abelson, 1978). substances may appear or disappear at different times in From the beginning, seeds have been and still are the the life of the cell. They are reserve or waste products main source of man’s diet. The Poaceae (large seeded resulting from cellular activities. Some well-known grasses) contribute more food seeds than any other plant ergastic substances are carbohydrates, cellulose and family; it contributes about 50% per capita energy intake starch, protein bodies, fats and related substances (FAD, 1977). (Eckey, 1954; Pritchard, 1997; Arahira et al., 1998; The importance of the nature of ergastic substances in Christina et al., 2001; Gómez-Sosa and Castro, 2004; plant taxonomy has been stressed by various authors Wolf, 2006). -
In Vitro Propagation of Digitalis Trojana Ivanina., an Endemic Medicinal Plant of Turkey Nurşen Çördük and Cüneyt Aki
Chapter In Vitro Propagation of Digitalis trojana Ivanina., an Endemic Medicinal Plant of Turkey Nurşen Çördük and Cüneyt Aki Abstract Digitalis trojana Ivanina is a member of the Plantaginaceae family and known by its common name, Helen of Troy foxglove. It is perennial endemic to Çanakkale and Balıkesir, northwestern Turkey. In order to develop an efficient shoot regen- eration protocol, the leaf explants of D. trojana were cultured on Murashige and Skoog (MS) medium containing 6-benzyl adenine (0.1, 0.5, 1.0, 3.0, 5.0 mg/L) and α-naphthalene acetic acid (0.1, 0.5, 1.0 mg/L), 3% (w/v) sucrose and 0.8% (w/v) agar. The highest number of regenerated shoots was obtained from leaf explants that were cultured on MS medium with 3.0 mg/L BA+0.1 mg/L NAA. Regenerated shoots were rooted on MS medium without plant growth regulators. Rooted plants (2–3 cm) were separately transferred to pots containing a mixture of peat and perlite (2:1 v/v) and acclimatized successfully in a growth chamber. Keywords: endemic, foxglove, in vitro, propagation, regeneration 1. Introduction Turkey has a rich biodiversity as a result of its location, its geological struc- ture and different climatic zones. Turkey hosts three biogeographical regions: Mediterranean, Euro-Siberian and Irano-Turanian. As a result of located on the meeting point of these three different regions, Turkey is one of the most important areas in the world in terms of biological diversity. It is one of the world’s richest countries with regard to diversity of plant species, hosting 167 families, 1320 genera and 9996 species [1]. -
Trophic Resource Use and Partitioning in Multispecies Ungulate Communities
Trophic resource use and partitioning in multispecies ungulate communities Robert Spitzer Faculty of Forest Sciences Department of Wildlife, Fish, and Environmental Studies Umeå Doctoral thesis Swedish University of Agricultural Sciences Umeå 2019 Acta Universitatis agriculturae Sueciae 2019:73 Cover: Annual diet composition of deer in Sweden (artworK: R. Spitzer) ISSN 1652-6880 ISBN (print version) 978-91-7760-464-8 ISBN (electronic version) 978-91-7760-465-5 © 2019 Robert Spitzer, Umeå Print: Original trycKeri, Umeå 2019 Trophic resource use and partitioning in multispecies ungulate communities Abstract Over the past decades, ungulates across the northern hemisphere have been expanding in range and numbers. This has raised concerns about their impacts, particularly on shared resources with humans, e.g., timber trees. Understanding how different ungulate species use trophic resources is therefore a crucial component of managing their populations. In this thesis, I synthesized data from the literature and used faecal DNA metabarcoding to investigate diets and patterns of resource partitioning for ungulate communities in Sweden and at the European scale. I also evaluated the reliability of dung morphometry for identifying ungulate species. I found that species identification of faecal pellets is difficult where similar-sized ungulates coexist which questions the reliability of pellet counts as a monitoring technique in such systems. Dung morphometry could, however, clearly distinguish moose from the smaller deer species. Across Europe, average diets of the four main deer species fit well with predictions by Hofmann’s hypothesis of ruminant feeding types. Red and fallow deer (mixed feeders) showed larger dietary plasticity than moose and roe deer (browsers). -
Unearthing Belowground Bud Banks in Fire-Prone Ecosystems
Unearthing belowground bud banks in fire-prone ecosystems 1 2 3 Author for correspondence: Juli G. Pausas , Byron B. Lamont , Susana Paula , Beatriz Appezzato-da- Juli G. Pausas 4 5 Glo'ria and Alessandra Fidelis Tel: +34 963 424124 1CIDE-CSIC, C. Naquera Km 4.5, Montcada, Valencia 46113, Spain; 2Department of Environment and Agriculture, Curtin Email [email protected] University, PO Box U1987, Perth, WA 6845, Australia; 3ICAEV, Universidad Austral de Chile, Campus Isla Teja, Casilla 567, Valdivia, Chile; 4Depto Ci^encias Biologicas,' Universidade de Sao Paulo, Av P'adua Dias 11., CEP 13418-900, Piracicaba, SP, Brazil; 5Instituto de Bioci^encias, Vegetation Ecology Lab, Universidade Estadual Paulista (UNESP), Av. 24-A 1515, 13506-900 Rio Claro, Brazil Summary To be published in New Phytologist (2018) Despite long-time awareness of the importance of the location of buds in plant biology, research doi: 10.1111/nph.14982 on belowground bud banks has been scant. Terms such as lignotuber, xylopodium and sobole, all referring to belowground bud-bearing structures, are used inconsistently in the literature. Key words: bud bank, fire-prone ecosystems, Because soil efficiently insulates meristems from the heat of fire, concealing buds below ground lignotuber, resprouting, rhizome, xylopodium. provides fitness benefits in fire-prone ecosystems. Thus, in these ecosystems, there is a remarkable diversity of bud-bearing structures. There are at least six locations where belowground buds are stored: roots, root crown, rhizomes, woody burls, fleshy -
Pondnet RECORDING FORM (PAGE 1 of 5)
WETLAND PLANTS PondNet RECORDING FORM (PAGE 1 of 5) Your Name Date Pond name (if known) Square: 4 fig grid reference Pond: 8 fig grid ref e.g. SP1243 e.g. SP 1235 4325 Determiner name (optional) Voucher material (optional) METHOD (complete one survey form per pond) Aim: To assess pond quality and conservation value, by recording wetland plants. How: Identify the outer boundary of the pond. This is the ‘line’ marking the pond’s highest yearly water levels (usually in early spring). It will probably not be the current water level of the pond, but should be evident from wetland vegetation like rushes at the pond’s outer edge, or other clues such as water-line marks on tree trunks or stones. Within the outer boundary, search all the dry and shallow areas of the pond that are accessible. Survey deeper areas with a net or grapnel hook. Record wetland plants found by crossing through the names on this sheet. You don’t need to record terrestrial species. For each species record its approximate abundance as a percentage of the pond’s surface area. Where few plants are present, record as ‘<1%’. If you are not completely confident in your species identification put ’?’ by the species name. If you are really unsure put ‘??’. Enter the results online: www.freshwaterhabitats.org.uk/projects/waternet/ or send your results to Freshwater Habitats Trust. Aquatic plants (submerged-leaved species) Nitella hyalina (Many-branched Stonewort) Floating-leaved species Apium inundatum (Lesser Marshwort) Nitella mucronata (Pointed Stonewort) Azolla filiculoides (Water Fern) Aponogeton distachyos (Cape-pondweed) Nitella opaca (Dark Stonewort) Hydrocharis morsus-ranae (Frogbit) Cabomba caroliniana (Fanwort) Nitella spanioclema (Few-branched Stonewort) Hydrocotyle ranunculoides (Floating Pennywort) Callitriche sp. -
Buglife Ditches Report Vol1
The ecological status of ditch systems An investigation into the current status of the aquatic invertebrate and plant communities of grazing marsh ditch systems in England and Wales Technical Report Volume 1 Summary of methods and major findings C.M. Drake N.F Stewart M.A. Palmer V.L. Kindemba September 2010 Buglife – The Invertebrate Conservation Trust 1 Little whirlpool ram’s-horn snail ( Anisus vorticulus ) © Roger Key This report should be cited as: Drake, C.M, Stewart, N.F., Palmer, M.A. & Kindemba, V. L. (2010) The ecological status of ditch systems: an investigation into the current status of the aquatic invertebrate and plant communities of grazing marsh ditch systems in England and Wales. Technical Report. Buglife – The Invertebrate Conservation Trust, Peterborough. ISBN: 1-904878-98-8 2 Contents Volume 1 Acknowledgements 5 Executive summary 6 1 Introduction 8 1.1 The national context 8 1.2 Previous relevant studies 8 1.3 The core project 9 1.4 Companion projects 10 2 Overview of methods 12 2.1 Site selection 12 2.2 Survey coverage 14 2.3 Field survey methods 17 2.4 Data storage 17 2.5 Classification and evaluation techniques 19 2.6 Repeat sampling of ditches in Somerset 19 2.7 Investigation of change over time 20 3 Botanical classification of ditches 21 3.1 Methods 21 3.2 Results 22 3.3 Explanatory environmental variables and vegetation characteristics 26 3.4 Comparison with previous ditch vegetation classifications 30 3.5 Affinities with the National Vegetation Classification 32 Botanical classification of ditches: key points -
THE IRISH RED DATA BOOK 1 Vascular Plants
THE IRISH RED DATA BOOK 1 Vascular Plants T.G.F.Curtis & H.N. McGough Wildlife Service Ireland DUBLIN PUBLISHED BY THE STATIONERY OFFICE 1988 ISBN 0 7076 0032 4 This version of the Red Data Book was scanned from the original book. The original book is A5-format, with 168 pages. Some changes have been made as follows: NOMENCLATURE has been updated, with the name used in the 1988 edition in brackets. Irish Names and family names have also been added. STATUS: There have been three Flora Protection Orders (1980, 1987, 1999) to date. If a species is currently protected (i.e. 1999) this is stated as PROTECTED, if it was previously protected, the year(s) of the relevant orders are given. IUCN categories have been updated as follows: EN to CR, V to EN, R to V. The original (1988) rating is given in brackets thus: “CR (EN)”. This takes account of the fact that a rare plant is not necessarily threatened. The European IUCN rating was given in the original book, here it is changed to the UK IUCN category as given in the 2005 Red Data Book listing. MAPS and APPENDIX have not been reproduced here. ACKNOWLEDGEMENTS We are most grateful to the following for their help in the preparation of the Irish Red Data Book:- Christine Leon, CMC, Kew for writing the Preface to this Red Data Book and for helpful discussions on the European aspects of rare plant conservation; Edwin Wymer, who designed the cover and who, as part of his contract duties in the Wildlife Service, organised the computer applications to the data in an efficient and thorough manner. -
Index Seminum 2017
ORTO BOTANICO DELL’UNIVERSITA’ DEGLI STUDI DI PALERMO INDEX SEMINUM MMXVII SPORAE ET SEMINA ANNI MMXVII QUAE PRO MUTUA COMMUTATIONE OFFERENTUR In copertina: veduta del Ginnasio (Schola Botanices) da una xilografia del 1832. Cover: view of Gymnasium (Schola Botanices) taken from a xylography of 1832. Il presente Index comprende, in due distinti elenchi, semi di piante spontanee raccolti durante il 2017 in varie località della Sicilia e di piante coltivate nell’Orto Botanico di Palermo. Nel rispetto della Convenzione sulla Biodiversità (Rio de Janeiro, 1992), i semi sono forniti alle seguenti condizioni che si ritengono accettate all’atto dell’ordinazione dei semi o di altro materiale vegetale: • il materiale deve essere usato per il bene comune nelle aree della ricerca, didattica, conservazione e sviluppo degli orti botanici; • se il richiedente intende commercializzare del materiale genetico o prodotti derivati, deve essere preventivamente autorizzato dall’Orto botanico di Palermo; • il materiale non può essere ceduto a terzi senza autorizzazione da parte dell’Orto botanico di Palermo; • ogni pubblicazione scientifica legata al materiale inviato, deve menzionare l’Orto botanico di Palermo come fornitore. L’Orto Botanico è posto a 10 m s.l.m. e si estende su una superficie di circa 10 ettari. Coordinate: 38.112N,13.374E. Per informazioni sul clima è possibile trarre dati aggiornati sul sito www.sias.regione.sicilia.it Le richieste di semi devono essere indirizzate via email a [email protected] indicando nell’oggetto: “Index Seminum – Desiderata 2017” e in calce l’esatto indirizzo presso il quale dovranno essere spediti i semi richiesti. This Index Seminum includes, in two separate lists, seeds of spontaneous plants collected during 2017 in various localities in Sicily and plants grown in the Botanical Garden of Palermo. -
Bocconea 25, Results of the Seventh Iter Mediterraneum
Bocconea 25: 5-127 doi: 10.7320/Bocc25.005 Version of Record published online on 9 July 2012 Werner Greuter Results of the Seventh “Iter Mediterraneum” in the Peloponnese, Greece, May to June 1995 (Occasional Papers from the Herbarium Greuter – N° 1) Abstract Greuter, W.: Results of the Seventh “Iter Mediterraneum” in the Peloponnese, Greece, May to June 1995. (Occasional Papers from the Herbarium Greuter – N° 1). — Bocconea. 25: 5-127. 2012. — ISSN 1120-4060 (print), 2280-3882 (online). The material collected during OPTIMA’s Iter Mediterraneum VII to the Peloponnese in 1995 has been revised. It comprises 2708 gatherings, each with 0 to 31 duplicates, collected in 53 numbered localities. The number of taxa (species or subspecies) represented is 1078. As many of the areas visited had been poorly explored before, a dozen of the taxa collected turned out to not to have been previously described, of which 9 (7 species, 2 subspecies) are described and named here (three more were published independently in the intervening years). They belong to the genera Allium, Asperula, Ballota, Klasea, Lolium, Minuartia, Nepeta, Oenanthe, and Trifolium. New combinations at the rank of subspecies (3) and variety (2) are also published. One of the species (Euphorbia aulacosperma) is first recorded for Europe, and several are new for the Peloponnese or had their known range of distribution significantly expanded. Critical notes draw attention to these cases and to taxonomic problems yet to be solved. An overview of the 11 Itinera Mediterranea that have taken place so far is presented, summarising their main results. Keywords: Flora of Greece, Peloponnese, Itinera Mediterranea, OPTIMA, new species, new com- binations, Allium, Asperula, Ballota, Klasea, Lolium, Minuartia, Nepeta, Oenanthe, Trifolium. -
Strategies for Conservation of Rare and Endemic
Strategies for Conservation of Rare and Endemic Species: Characterization of Genetic and Epigenetic Variation and Unusual Reproductive Biology of Coastal Species from Limonium ovalifolium and Limonium binervosum Complexes (Plumbaginaceae) TESE APRESENTADA PARA OBTENÇÃO DO GRAU DE DOUTOR EM BIOLOGIA ANA SOFIA DA SILVA VALBORDO RÓIS ORIENTADORA: Dr. Ana Cristina Delaunay Caperta COORIENTADOR: Dr. Timothy F. Sharbel Júri: Presidente: Reitor da Universidade de Lisboa Vogais: Doutora Maria Wanda Sarujine Viegas Professora Catedrática Instituto Superior de Agronomia da Universidade de Lisboa Doutor João Carlos Mano Castro Loureiro Professor Auxiliar Faculdade de Ciências e Tecnologias da Universidade de Coimbra Doutor Octávio Fernando de Sousa Salgueiro Godinho Paulo Professor Auxiliar Faculdade de Ciências da Universidade de Lisboa Doutor Jorge Henrique Capelo Gonçalves Investigador Auxiliar Instituto Nacional de Investigação Agrária e Veterinária Doutora Ana Cristina Delaunay Caperta Investigadora Auxiliar Instituto Superior de Agronomia da Universidade de Lisboa LISBOA 2014 Strategies for Conservation of Rare and Endemic Species: Characterization of Genetic and Epigenetic Variation and Unusual Reproductive Biology of Coastal Species from Limonium ovalifolium and Limonium binervosum Complexes (Plumbaginaceae) TESE APRESENTADA PARA OBTENÇÃO DO GRAU DE DOUTOR EM BIOLOGIA ANA SOFIA DA SILVA VALBORDO RÓIS ORIENTADORA: Dr. Ana Cristina Delaunay Caperta COORIENTADOR: Dr. Timothy F. Sharbel Júri: Presidente: Reitor da Universidade de Lisboa Vogais: