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RBM3 Antibody Cat. No.: 19-949 RBM3 Antibody Immunohistochemistry of paraffin- embedded rat brain using RBM3 Immunohistochemistry of paraffin-embedded human endometrium cancer antibody (19-949) at dilution of 1:100 using RBM3 antibody (19-949) at dilution of 1:100 (40x lens). (40x lens). Immunohistochemistry of paraffin- embedded mouse brain using RBM3 antibody (19-949) at dilution of 1:100 (40x lens). Specifications September 25, 2021 1 https://www.prosci-inc.com/rbm3-antibody-19-949.html HOST SPECIES: Rabbit SPECIES REACTIVITY: Human, Mouse, Rat Recombinant fusion protein containing a sequence corresponding to amino acids 1-157 of IMMUNOGEN: human RBM3 (NP_006734.1). TESTED APPLICATIONS: IF, IHC, WB WB: ,1:200 - 1:2000 APPLICATIONS: IHC: ,1:50 - 1:200 IF: ,1:50 - 1:200 POSITIVE CONTROL: 1) HeLa 2) Jurkat 3) HL-60 PREDICTED MOLECULAR Observed: 17kDa WEIGHT: Properties PURIFICATION: Affinity purification CLONALITY: Polyclonal ISOTYPE: IgG CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles. Additional Info OFFICIAL SYMBOL: RBM3 RNA binding motif protein 3, OTTHUMP00000025802, OTTHUMP00000025800, RNPL, IS1- ALTERNATE NAMES: RNPL, RNA binding motif (RNP1, RRM) protein 3, RBM3 GENE ID: 5935 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References September 25, 2021 2 https://www.prosci-inc.com/rbm3-antibody-19-949.html This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple BACKGROUND: alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 25, 2021 3 https://www.prosci-inc.com/rbm3-antibody-19-949.html.

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Anti-RBM3 Antibody [HB9] (ARG23691)
Product datasheet [email protected] ARG23691 Package: 50 μg anti-RBM3 antibody [HB9] Store at: -20°C Summary Product Description Mouse Monoclonal antibody [HB9] recognizes RBM3 Tested Reactivity Hu Tested Application ICC/IF Specificity The antibody reacts against the recombinant human RBM3 full length under oxidative conditions without DTT or under reducing conditions with DTT. Cross reactivity of the antibody with the recombinant human Cold inducible Binding protein (CIRBP, full length 20.5 kDa) could not be detected. The apparent MW of hRBM3 in SDS-PAGE is ~17 kDa. Host Mouse Clonality Monoclonal Clone HB9 Isotype IgG1 Target Name RBM3 Antigen Species Human Immunogen Human RNA-binding protein 3. Conjugation Un-conjugated Alternate Names RNPL; IS1-RNPL; RNA-binding motif protein 3; RNA-binding protein 3 Application Instructions Application table Application Dilution ICC/IF 1:50 Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 17 kDa Properties Form Liquid Purification Purification with Protein G. The IgG1 fraction was purified by affinity chromatography. Buffer PBS (pH 7.4) and 0.01% Sodium azide. Preservative 0.01% Sodium azide Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.
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Identification of the Rna Binding Protein Rbm3 As a Novel Effector of Β-Catenin Signaling and Colon Cancer Stem Cells
IDENTIFICATION OF THE RNA BINDING PROTEIN RBM3 AS A NOVEL EFFECTOR OF β-CATENIN SIGNALING AND COLON CANCER STEM CELLS By Anand Venugopal Submitted to the graduate degree program in Molecular and Integrative Physiology and the Graduate Faculty of the University of Kansas in partial fulfillment of the requirements for the degree of Doctor of Philosophy. ________________________________ Chairperson Shrikant Anant, Ph.D. ________________________________ Roy Jensen, M.D. ________________________________ Andrew Godwin, Ph.D. ________________________________ Danny Welch, Ph.D. ________________________________ John Wood, Ph.D. Date Defended: ________________________________March 14, 2014 The Dissertation Committee for Anand Venugopal certifies that this is the approved version of the following dissertation: IDENTIFICATION OF THE RNA BINDING PROTEIN RBM3 AS A NOVEL EFFECTOR OF β-CATENIN SIGNALING AND COLON CANCER STEM CELLS ________________________________ Chairperson Shrikant Anant, Ph.D. Date approved: ________________________________March 14, 2014 ii Abstract The intestinal epithelium is one of the fastest renewing tissues within the adult. This renewal is primarily driven by the intestinal epithelial stem cell compartment and homeostasis of this compartment needs to be strictly maintained. Loss of regulation can lead to hyperplasia and subsequent malignant transformation. Moreover, cancers are also thought to contain a stem cell population which maintains long term tumor viability and recurrence following therapy. Therefore, a thorough understanding of the molecular machinery that governs stem cell homeostasis can further our understanding of colon cancer initiation and progression. The RNA binding protein RBM3 is upregulated in many solid tumors including colon, prostate and breast. It also serves as a proto-oncogene inducing the malignant transformation of normal cells when overexpressed. To further characterize the mechanism, we overexpressed RBM3 in DLD-1 and HCT 116 colon cancer cell lines.
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Human RBM3 ELISA Kit (ARG81349)
Product datasheet [email protected] ARG81349 Package: 96 wells Human RBM3 ELISA Kit Store at: 4°C Summary Product Description ARG81349 Human RBM3 ELISA Kit is an Enzyme Immunoassay kit for the quantification of Human RBM3 in serum and plasma. Tested Reactivity Hu Tested Application ELISA Specificity No significant cross reactivity to Cold inducible Binding protein (CIRBP), human. Target Name RBM3 Conjugation HRP Conjugation Note Substrate: TMB and read at 450 nm. Sensitivity 10 pg/ml Sample Type Serum and plasma. Standard Range 31.25 - 2000 pg/ml Sample Volume 50 µl Alternate Names RNPL; IS1-RNPL; RNA-binding motif protein 3; RNA-binding protein 3 Application Instructions Assay Time ~ 2.5 hours Properties Form 96 well Storage instruction Store the kit at 2-8°C. Keep microplate wells sealed in a dry bag with desiccants. Do not expose test reagents to heat, sun or strong light during storage and usage. Please refer to the product user manual for detail temperatures of the components. Note For laboratory research only, not for drug, diagnostic or other use. Bioinformation Gene Symbol RBM3 Gene Full Name RNA binding motif (RNP1, RRM) protein 3 Background This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria.
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Human RBM3 ELISA Assay Kit
Human RBM3 ELISA Assay Kit BTRBM-001 For quantitative determination of human RNA-binding protein 3 96 Determinations 2°C – 8 °C For research use only Not for diagnostic use RBM3 ELISA Assay Kit 1 of 14 Catalog Number: BTRBM001 www.EagleBio.com Contents Short Review 3 Intended Use 4 Test principle 5 Safety warning and precautions 5 Storage 6 Components of the assay system 6 Sample preparation and storage 7 Critical parameters 7 Preparation of reagents 8 Assay protocol 8 Protocol summary 9 Scheme of the plate 10 Data processing 10 Additional information 11 Specificity 11 Sensitivity 11 Linearity 11 Recovery 11 Reproducibility 12 Troubleshooting 13 Related Products 14 BioTeZ Berlin-Buch GmbH Robert-Rössle-Str. 10, Haus 72 13125 Berlin Tel.: ++49 (0)30-9489 3322 Fax: ++49 (0)30-949 2008 e-mail: [email protected] http://www.biotez.de This manual is valid from May, 2016 RBM3 ELISA Assay Kit 2 of 14 Catalog Number: BTRBM001 www.EagleBio.com Short Review The RNA-binding protein 3 (RBM3) is a member of the glycine-rich RNA-binding protein family. The exact role of the cold shock protein RBM3 is not yet clear [2, 6]. The role of mRNA-binding proteins can be attributed to a regulation of mRNA splicing, stability, transport and ultimately the translation of mRNA into proteins. RBM3 consists of 157 amino acids with a predicted mass of 17 kD (1, 2) and is one of the few proteins that are upregulated at low temperature [3-5]. It is known that RBM3 acts especially in cellular stress (eg.
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Molecular Interactions Underpinning the Phenotype of Hibernation in Mammals Matthew T
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Oxygen-Regulated Expression of the RNA-Binding Proteins RBM3 and CIRP by a HIF-1-Independent Mechanism
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RBM3: a Prognostic and Treatment Predictive Biomarker
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Effect of Temperature Downshift on the Transcriptomic Responses of Chinese Hamster Ovary Cells Using Recombinant Human Tissue Plasminogen Activator Production Culture
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Impact of RNA‑Binding Motif 3 Expression on the Whole Transcriptome of Prostate Cancer Cells: an RNA Sequencing Study
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RBMX Family Proteins Connect the Fields of Nuclear RNA Processing
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(RBM3) Regulates Type 2 Innate Lymphoid Cell (ILC2) Cytokine Production
UNIVERSITY OF CALIFORNIA, SAN DIEGO RNA-Binding Motif 3 (RBM3) regulates Type 2 Innate Lymphoid cell (ILC2) Cytokine Production A Thesis Submitted in Partial Satisfaction of the Requirements for the Degree Master of Science in Biology by Jana Hicham Badrani Committee in charge: Taylor A. Doherty, Chair Li-Fan Lu, Co-Chair James Cooke 2017 Copyright Jana Hicham Badrani, 2017 All rights reserved. The Thesis of Jana Hicham Badrani is approved, and it is acceptable in quality and form for publication on microfilm and electronically: Co-Chair Chair University of California, San Diego 2017 iii DEDICATION This work is dedicated to my family: my parents, for their unwavering faith and drive, and my siblings, for their never ending curiosity and constant criticism. My parents have constantly stood by me and have always supported my dreams and desires. They have provided me with an appreciation of hard work and the drive to progress further than I ever could alone. They are, and always will be, my inspiration, my motivation, and my best friends. My younger siblings are constant reminders of life’s simple pleasures. They have shown me how far an active imagination can take you and have taught me all I know about patience. iv TABLE OF CONTENTS Signature Page…………………………………………………...……………………….iii Dedication………………………………………………………………………………...iv Table of Contents………………………………………………………………………….v List of Figures………………………………………………………………………….…vi Acknowledgements………………………………………………………………………vii Abstract of the Thesis…………………………………………………………………...viii Introduction………………………………………………………………………………..1
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