Anti-RBM3 Antibody [HB9] (ARG23691)

ARG23691 Package: 50 μg anti-RBM3 antibody [HB9] Store at: -20°C

Summary

Product Description Mouse Monoclonal antibody [HB9] recognizes RBM3

Tested Reactivity Hu

Tested Application ICC/IF

Specificity The antibody reacts against the recombinant human RBM3 full length under oxidative conditions without DTT or under reducing conditions with DTT. Cross reactivity of the antibody with the recombinant human Cold inducible Binding protein (CIRBP, full length 20.5 kDa) could not be detected. The apparent MW of hRBM3 in SDS-PAGE is ~17 kDa.

Host Mouse

Clonality Monoclonal

Clone HB9

Isotype IgG1

Target Name RBM3

Antigen Species Human

Immunogen Human RNA-binding protein 3.

Conjugation Un-conjugated

Alternate Names RNPL; IS1-RNPL; RNA-binding motif protein 3; RNA-binding protein 3

Application Instructions

Application table Application Dilution

ICC/IF 1:50

Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist.

Calculated Mw 17 kDa

Properties

Form Liquid

Purification Purification with Protein G. The IgG1 fraction was purified by affinity chromatography.

Buffer PBS (pH 7.4) and 0.01% Sodium azide.

Preservative 0.01% Sodium azide

Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C or below. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use.

www.arigobio.com 1/2 Note For laboratory research only, not for drug, diagnostic or other use.

Bioinformation

Gene Symbol RBM3

Gene Full Name RNA binding motif (RNP1, RRM) protein 3

Background This gene is a member of the glycine-rich RNA-binding protein family and encodes a protein with one RNA recognition motif (RRM) domain. Expression of this gene is induced by cold shock and low oxygen tension. A pseudogene exists on chromosome 1. Multiple alternatively spliced transcript variants that are predicted to encode different isoforms have been characterized although some of these variants fit nonsense-mediated decay (NMD) criteria. [provided by RefSeq, Jul 2008]

Function Cold-inducible mRNA binding protein that enhances global protein synthesis at both physiological and mild hypothermic temperatures. Reduces the relative abundance of microRNAs, when overexpressed. Enhances phosphorylation of translation initiation factors and active polysome formation (By similarity). [UniProt]

Images

ARG23691 anti-RBM3 antibody [HB9] ICC/IF image

Immunofluorescence: Mouse primary hippocampal neurons isolate cells stained with ARG23691 anti-RBM3 antibody [HB9] (green) at 1:50, counter-stained with DAPI at 1:2000. The cells were fixed in 4% Paraformaldehyde/PBS, blocked and permeabilized using 3% BSA + 0.1% Triton X.

ARG23691 anti-RBM3 antibody [HB9] WB image

Western blot: Left Panel: 20 or 100 ng of hRBM3 full length recombinant proteins stained with ARG23691 anti-RBM3 antibody [HB9] under Oxidative or Reducing conditions. Right Panel: 20 or 100 ng of hCIRBP full length recombinant proteins stained with ARG23691 anti-RBM3 antibody [HB9] under Oxidative or Reducing conditions.

Oxidative conditions: Without DTT, without incubation at 95°C Reducing conditions: With DTT, 10 min. Incubation at 95°C