animals Article Preference and Motivation Tests for Body Tactile Stimulation in Fish Ana Carolina dos Santos Gauy 1,2, Marcela Cesar Bolognesi 1,2, Guilherme Delgado Martins 1 and Eliane Gonçalves-de-Freitas 1,2,* 1 Departamento de Ciências Biológicas, Instituto de Biociências, Letras e Ciências Exatas, Universidade Estadual Paulista (UNESP), Rua Cristóvão Colombo, 2265, São José do Rio Preto 15054-000, SP, Brazil;
[email protected] (A.C.d.S.G.);
[email protected] (M.C.B.);
[email protected] (G.D.M.) 2 CAUNESP—Centro de Aquicultura da UNESP, Jaboticabal 14884-900, SP, Brazil * Correspondence:
[email protected] Simple Summary: Body tactile stimulation, such as human massage therapy, is a way to relieve stress in humans and other animals, therefore it could improve animal health and welfare. This physical stimulation can also be done through artificial devices, as a sensory enrichment. However, before using it in an artificial environment, it is imperative to test whether animals perceive such enrichment as positive (searching for it spontaneously) or negative (avoiding it). Here, we tested whether the Nile tilapia fish search for or avoid tactile stimulation. We used a rectangular PVC frame, filled with vertical plastic sticks sided with silicone bristles that provided tactile stimulation when fish passed through them. We carried out preference and motivation tests, in which fish could choose to cross through the device with and without tactile stimulus. The same procedure was repeated after fish were exposed to either isolation or social stress. We found that fish crossed less by tactile device than by open areas.