Degradation of Microcystins Using Immobilized Microorganism Isolated in an Eutrophic Lake
Chemosphere 65 (2006) 117–124 www.elsevier.com/locate/chemosphere Degradation of microcystins using immobilized microorganism isolated in an eutrophic lake Kiyomi Tsuji a,*, Miki Asakawa a, Yojiro Anzai b, Tatsuo Sumino c, Ken-ichi Harada d a Kanagawa Prefectural Institute of Public Health (Formerly, Kanagawa Prefectural Public Health Laboratory), 1-3-1 Shimomachiya, Chigasaki, Kanagawa 253-0087, Japan b Faculty of Pharmaceutical Sciences, Toho University, Miyama, Funabashi, Chiba 274-8510, Japan c Research Division, Hitachi Plant Engineering and Construction Corporation, Kamihongo 537, Matsudo, Chiba 271-0064, Japan d Graduate School of Environmental and Human Sciences and Faculty of Pharmacy, Meijo University, Tempaku, Nagoya 468-8503, Japan Received 28 September 2005; received in revised form 14 February 2006; accepted 14 February 2006 Available online 24 March 2006 Abstract The final purpose of our series of studies is to establish a biological removal method of cyanobacteria and their toxic products using immobilized microorganisms that can lyse cyanobacteria and decompose microcystins. To establish the biological removal method in non-point areas and water purification plants, as the first step, we explored bacteria active against the cyanobacterial hepatotoxin micro- cystin in the present study. Eleven active bacteria were isolated from samples taken from Lakes Tsukui and Sagami, Japan. Among 3 strains (B-9 to B-11) with degradative activity, strain B-9 exhibited the strongest activity. The 16S rDNA sequence of the strain B-9 showed the highest similarity to that of Sphingomonas sp. Y2 (AB084247, 99% similarity). Microcystins-RR and -LR were completely degraded by strain B-9 (SC16) within 1 d, which led to an immobilized microorganism with a polyester resin.
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