RBM25 Polyclonal Antibody

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PRODUCT DATA SHEET Bioworld Technology,Inc. RBM25 polyclonal antibody Catalog: BS72716 Host: Rabbit Reactivity: Human,Mouse BackGround: munogen and the purity is > 95% (by SDS-PAGE). RMB25 (RNA binding motif protein 25), also known as Applications: S164, RNPC7, RED120 or fSAP94, is a 784 amino acid WB 1:200 - 1:2000 protein that contains one PWI domain and one RRM Storage&Stability: (RNA recognition motif) domain. Localized to the cyto- Store at 4°C short term. Aliquot and store at -20°C long plasm, as well as to nuclear speckles, RBM25 functions term. Avoid freeze-thaw cycles. as a splicing factor that is thought to stabilize the Specificity: pre-mRNA-U1 snRNP complex and may couple splicing RBM25 polyclonal antibody detects endogenous levels of with mRNA 3'-end formation. The gene encoding RBM25 protein. RBM25 maps to human chromosome 14, which houses DATA: over 700 genes and comprises nearly 3.5% of the human genome. Chromosome 14 encodes the presinilin 1 (PSEN1) gene, which is one of the three key genes asso- ciated with the development of Alzheimer's disease (AD). Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2 Molecular Weight: Western blot analysis of extracts of various cell lines, using RBM25 an- ~ 120 kDa tibody. Swiss-Prot: Note: P49756 For research use only, not for use in diagnostic procedure. Purification&Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific im- Bioworld Technology, Inc. Bioworld technology, co. Ltd. Add： 1660 South Highway 100, Suite 500 St. Louis Park, Add： No 9, weidi road Qixia District Nanjing, 210046, MN 55416,USA. P. R. China. Email: [email protected] Email: [email protected] Tel: 6123263284 Tel: 0086-025-68037686 Fax: 6122933841 Fax: 0086-025-68035151 .

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RNA Splicing Regulated by RBFOX1 Is Essential for Cardiac Function in Zebrafish Karen S
© 2015. Published by The Company of Biologists Ltd | Journal of Cell Science (2015) 128, 3030-3040 doi:10.1242/jcs.166850 RESEARCH ARTICLE RNA splicing regulated by RBFOX1 is essential for cardiac function in zebrafish Karen S. Frese1,2,*, Benjamin Meder1,2,*, Andreas Keller3,4, Steffen Just5, Jan Haas1,2, Britta Vogel1,2, Simon Fischer1,2, Christina Backes4, Mark Matzas6, Doreen Köhler1, Vladimir Benes7, Hugo A. Katus1,2 and Wolfgang Rottbauer5,‡ ABSTRACT U2, U4, U5 and U6) and several hundred associated regulator Alternative splicing is one of the major mechanisms through which proteins (Wahl et al., 2009). Some of the best-characterized the proteomic and functional diversity of eukaryotes is achieved. splicing regulators include the serine-arginine (SR)-rich family, However, the complex nature of the splicing machinery, its associated heterogeneous nuclear ribonucleoproteins (hnRNPs) proteins, and splicing regulators and the functional implications of alternatively the Nova1 and Nova2, and the PTB and nPTB (also known as spliced transcripts are only poorly understood. Here, we investigated PTBP1 and PTBP2) families (David and Manley, 2008; Gabut et al., the functional role of the splicing regulator rbfox1 in vivo using the 2008; Li et al., 2007; Matlin et al., 2005). The diversity in splicing is zebrafish as a model system. We found that loss of rbfox1 led to further increased by the location and nucleotide sequence of pre- progressive cardiac contractile dysfunction and heart failure. By using mRNA enhancer and silencer motifs that either promote or inhibit deep-transcriptome sequencing and quantitative real-time PCR, we splicing by the different regulators. Adding to this complexity, show that depletion of rbfox1 in zebrafish results in an altered isoform regulating motifs are very common throughout the genome, but are expression of several crucial target genes, such as actn3a and hug.
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Human Proteins That Interact with RNA/DNA Hybrids
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1 SUPPLEMENTAL DATA Figure S1. Poly I:C Induces IFN-Β Expression
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RBM25 Sirna (H): Sc-92256
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