Phylum Ciliata

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PHYLUM CILIATA 1.a. Find a culture of Dileptus and examine it in a thick wet mount. Carefully describe the behavior of the organism. 1.b. Label the neck, cytostome, and pellicle of the outline drawing of Dileptus. 1.c. Add toxicysts, cilia, contractile vacuoles to the illustration above. 2. Examine living Didinium and describe the pattern of cilia on the cell. 3.a. Balantidium coli is a parasite of the human colon and causes balantidial dysentery. Find the demonstration slide of Balantidium and compare its cytostome with that of Didinium? 3.b. Sketch the macronucleus and micronucleus. 4.a. Examine living Paramecium cells which have been mixed with homogenized milk stained with Congo Red. Only the milk fat droplets take up the stain. Follow the stained droplets that have been ingested by the Paramecium. How does the color change? [Congo Red is red in a basic and blue in an acidic medium.] 4.b. While looking for color changes in the food vacuoles, observe the action of the contractile vacuoles and cilia at high magnification. Sketch a contractile vacuole when fully distended and contracted. 4.c. Now carefully scan the cell surface. Are all cilia that cover a Paramecium uniform in size? 4.d. Look for examples of cell division. How does fission differ from conjugation in the ciliates? 4.e. Add a drop of 1% acetic acid to the edge of the coverslip and watch a cell's response to the acetic acid. What happened? 4.f Find the oral groove, cytostome, oral membranes, macronucleus, and micronucleus and add them to the drawing below. 5.a. Mount living Vorticella on a slide and find the sessile cells. Tap the microscope stage until the cells are disturbed. Describe the behavior of the cells as they respond to the tapping? 5.b. How are cilia distributed of the Vorticella? 5.c. Do you see any motile forms? 6.a. Examine living Stentor in a wet mount. In what way genus similar to Vorticella? 6.b. In what important ways are they different? 6.c. In what kind of aquatic habitat would you expect to find both Vorticella and Stentor? 7.a. How does Euplotes differ from Paramecium and Stentor in the form of its cell and its ciliation? 7.b. Carefully describe the behavior of Euplotes. 8. Carefully examine the demonstration slide of rumen ciliates. Describe the ciliation of these cells. 9.a. Find the demonstration slide of Ephelota. Although this organism looks like Vorticella, it feeds in a very different way. How? 9.b. How is dispersal accomplished in the suctorians? .

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Balantidium Coli
GLOBAL WATER PATHOGEN PROJECT PART THREE. SPECIFIC EXCRETED PATHOGENS: ENVIRONMENTAL AND EPIDEMIOLOGY ASPECTS BALANTIDIUM COLI Francisco Ponce-Gordo Complutense University Madrid, Spain Kateřina Jirků-Pomajbíková Institute of Parasitology Biology Centre, ASCR, v.v.i. Budweis, Czech Republic Copyright: This publication is available in Open Access under the Attribution-ShareAlike 3.0 IGO (CC-BY-SA 3.0 IGO) license (http://creativecommons.org/licenses/by-sa/3.0/igo). By using the content of this publication, the users accept to be bound by the terms of use of the UNESCO Open Access Repository (http://www.unesco.org/openaccess/terms-use-ccbysa-en). Disclaimer: The designations employed and the presentation of material throughout this publication do not imply the expression of any opinion whatsoever on the part of UNESCO concerning the legal status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. The ideas and opinions expressed in this publication are those of the authors; they are not necessarily those of UNESCO and do not commit the Organization. Citation: Ponce-Gordo, F., Jirků-Pomajbíková, K. 2017. Balantidium coli. In: J.B. Rose and B. Jiménez-Cisneros, (eds) Global Water Pathogens Project. http://www.waterpathogens.org (R. Fayer and W. Jakubowski, (eds) Part 3 Protists) http://www.waterpathogens.org/book/balantidium-coli Michigan State University, E. Lansing, MI, UNESCO. Acknowledgements: K.R.L. Young, Project Design editor; Website Design (http://www.agroknow.com) Published: January 15, 2015, 11:50 am, Updated: October 18, 2017, 5:43 pm Balantidium coli Summary 1.1.1 Global distribution Balantidium coli is reported worldwide although it is To date, Balantidium coli is the only ciliate protozoan more common in temperate and tropical regions (Areán and reported to infect the gastrointestinal track of humans.
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