Can Protozoa Prove the Beginning of the World?
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The Morphology, Ultrastructure and Molecular Phylogeny of a New Freshwater Heterolobose Amoeba Parafumarolamoeba Stagnalis N. Sp
diversity Article The Morphology, Ultrastructure and Molecular Phylogeny of a New Freshwater Heterolobose Amoeba Parafumarolamoeba stagnalis n. sp. (Vahlkampfiidae; Heterolobosea) Anastasia S. Borodina 1,2, Alexander P. Mylnikov 1,†, Jan Janouškovec 3 , Patrick J. Keeling 4 and Denis V. Tikhonenkov 1,5,* 1 Papanin Institute for Biology of Inland Waters, Russian Academy of Sciences, 152742 Borok, Russia; [email protected] 2 Department of Zoology and Parasitology, Voronezh State University, Universitetskaya Ploshad 1, 394036 Voronezh, Russia 3 Centre Algatech, Laboratory of Photosynthesis, Institute of Microbiology, Czech Academy of Sciences, Opatovický Mlýn, 37981 Tˇreboˇn,Czech Republic; [email protected] 4 Department of Botany, University of British Columbia, 6270 University Boulevard, Vancouver, BC V6T1Z4, Canada; [email protected] 5 AquaBioSafe Laboratory, University of Tyumen, 625003 Tyumen, Russia * Correspondence: [email protected]; Tel.: +7-485-472-4533 † Alexander P. Mylnikov is deceased. http://zoobank.org/References/e543a49a-16c1-4b7c-afdb-0bc56b632ef0 Abstract: Heterolobose amoebae are important members of marine, freshwater, and soil microbial Citation: Borodina, A.S.; Mylnikov, communities, but their diversity remains under-explored. We studied the diversity of Vahlkampfiidae A.P.; Janouškovec, J.; Keeling, P.J.; to improve our understanding of heterolobosean relationships and their representation in aquatic Tikhonenkov, D.V. The Morphology, benthos. Using light and electron microscopy, and molecular phylogenies based on the SSU rRNA Ultrastructure and Molecular and ITS loci, we describe the fine morphology and evolutionary relationships of a new heterolobosean Phylogeny of a New Freshwater Parafumarolamoeba stagnalis n. sp. from a small pond in European Russia. Cells of P. stagnalis possess Heterolobose Amoeba a clearly distinguishable anterior hyaline pseudopodium, eruptive movement, several thin and Parafumarolamoeba stagnalis n. -
Swimming with Protists: Perception, Motility and Flagellum Assembly
REVIEWS Swimming with protists: perception, motility and flagellum assembly Michael L. Ginger*, Neil Portman‡ and Paul G. McKean* Abstract | In unicellular and multicellular eukaryotes, fast cell motility and rapid movement of material over cell surfaces are often mediated by ciliary or flagellar beating. The conserved defining structure in most motile cilia and flagella is the ‘9+2’ microtubule axoneme. Our general understanding of flagellum assembly and the regulation of flagellar motility has been led by results from seminal studies of flagellate protozoa and algae. Here we review recent work relating to various aspects of protist physiology and cell biology. In particular, we discuss energy metabolism in eukaryotic flagella, modifications to the canonical assembly pathway and flagellum function in parasite virulence. Protists The canonical ‘9+2’ microtubule axoneme is the prin- of inherited pathologies (or ciliopathies), including Eukaryotes that cannot be cipal feature of many motile cilia and flagella and is infertility, chronic respiratory disease, polycystic kid- classified as animals, fungi or one of the most iconic structures in cell biology. The ney disease and syndromes that include Bardet–Biedl, plants. The kingdom Protista origin of the eukaryotic flagellum and cilium is ancient Alstrom and Meckel syndrome6–12. Even illnesses such includes protozoa and algae. and predates the radiation, over 800 million years ago, as cancer, diabetes and obesity have been linked to cili- 1 6 Ciliates of the lineages that gave rise to extant eukaryotes . ary defects . The biological basis for some ciliopathies A ubiquitous group of protists, Therefore the absence of cilia and flagella from yeast, is undoubtedly defective sensing (an essential function members of which can be many fungi, red algae and higher plants are all examples provided by cilia), rather than defects in the assembly found in many wet of secondary loss. -
Ciliary Chemosensitivity Is Enhanced by Cilium Geometry and Motility
bioRxiv preprint doi: https://doi.org/10.1101/2021.01.13.425992; this version posted January 14, 2021. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Ciliary chemosensitivity is enhanced by cilium geometry and motility David Hickey,1 Andrej Vilfan,1, 2, ∗ and Ramin Golestanian1, 3 1Max Planck Institute for Dynamics and Self-Organization (MPIDS), 37077 G¨ottingen,Germany 2JoˇzefStefan Institute, 1000 Ljubljana, Slovenia 3Rudolf Peierls Centre for Theoretical Physics, University of Oxford, Oxford OX1 3PU, United Kingdom (Dated: January 13, 2021) Cilia are hairlike organelles involved in both sensory functions and motility. We discuss the question of whether the location of chemical receptors on cilia provides an advantage in terms of sensitivity. Using a simple advection-diffusion model, we compute the capture rates of diffusive molecules on a cilium. Because of its geometry, a non-motile cilium in a quiescent fluid has a capture rate equivalent to a circular absorbing region with ∼ 4× its surface area. When the cilium is exposed to an external shear flow, the equivalent surface area increases to ∼ 10×. Alternatively, if the cilium beats in a non-reciprocal way, its capture rate increases with the beating frequency to the power of 1=3. Altogether, our results show that the protruding geometry of a cilium could be one of the reasons why so many receptors are located on cilia. They also point to the advantage of combining motility with chemical reception. -
Unfolding the Secrets of Coral–Algal Symbiosis
The ISME Journal (2015) 9, 844–856 & 2015 International Society for Microbial Ecology All rights reserved 1751-7362/15 www.nature.com/ismej ORIGINAL ARTICLE Unfolding the secrets of coral–algal symbiosis Nedeljka Rosic1, Edmund Yew Siang Ling2, Chon-Kit Kenneth Chan3, Hong Ching Lee4, Paulina Kaniewska1,5,DavidEdwards3,6,7,SophieDove1,8 and Ove Hoegh-Guldberg1,8,9 1School of Biological Sciences, The University of Queensland, St Lucia, Queensland, Australia; 2University of Queensland Centre for Clinical Research, The University of Queensland, Herston, Queensland, Australia; 3School of Agriculture and Food Sciences, The University of Queensland, St Lucia, Queensland, Australia; 4The Kinghorn Cancer Centre, Garvan Institute of Medical Research, Sydney, New South Wales, Australia; 5Australian Institute of Marine Science, Townsville, Queensland, Australia; 6School of Plant Biology, University of Western Australia, Perth, Western Australia, Australia; 7Australian Centre for Plant Functional Genomics, The University of Queensland, St Lucia, Queensland, Australia; 8ARC Centre of Excellence for Coral Reef Studies, The University of Queensland, St Lucia, Queensland, Australia and 9Global Change Institute and ARC Centre of Excellence for Coral Reef Studies, The University of Queensland, St Lucia, Queensland, Australia Dinoflagellates from the genus Symbiodinium form a mutualistic symbiotic relationship with reef- building corals. Here we applied massively parallel Illumina sequencing to assess genetic similarity and diversity among four phylogenetically diverse dinoflagellate clades (A, B, C and D) that are commonly associated with corals. We obtained more than 30 000 predicted genes for each Symbiodinium clade, with a majority of the aligned transcripts corresponding to sequence data sets of symbiotic dinoflagellates and o2% of sequences having bacterial or other foreign origin. -
There Is Not a Latin Root Word Clear Your Desk Protist Quiz Grade Quiz
There is not a Latin Root Word Clear your desk Protist Quiz Grade Quiz Malaria Fever Wars Classification Kingdom Protista contains THREE main groups of organisms: 1. Protozoa: “animal-like protists” 2. Algae: “plant-like protists” 3. Slime & Water Molds: “fungus-like protists” Basics of Protozoa Unicellular Eukaryotic unlike bacteria 65, 000 different species Heterotrophic Free-living (move in aquatic environments) or Parasitic Habitats include oceans, rivers, ponds, soil, and other organisms. Protozoa Reproduction ALL protozoa can use asexual reproduction through binary fission or multiple fission FEW protozoa reproduce sexually through conjugation. Adaptation Special Protozoa Adaptations Eyespot: detects changes in the quantity/ quality of light, and physical/chemical changes in their environment Cyst: hardened external covering that protects protozoa in extreme environments. Basics of Algae: “Plant-like” protists. MOST unicellular; SOME multicellular. Make food by photosynthesis (“autotrophic prostists”). Were classified as plants, BUT… – Lack tissue differentiation- NO roots, stems, leaves, etc. – Reproduce differently Most algal cells have pyrenoids (organelles that make and store starch) Can use asexual or sexual reproduction. Algae Structure: Thallus: body portion; usually haploid Body Structure: 1) unicellular: single-celled; aquatic (Ex.phytoplankton, Chlamydomonas) 2) colonial: groups of coordinated cells; “division of labor” (Ex. Volvox) 3) filamentous: rod-shaped thallus; some anchor to ocean bottom (Ex. Spyrogyra) 4) multicellular: large, complex, leaflike thallus (Ex. Macrocystis- giant kelp) Basics of Fungus-like Protists: Slime Molds: Water Molds: Once classified as fungi Fungus-like; composed of Found in damp soil, branching filaments rotting logs, and other Commonly freshwater; decaying matter. some in soil; some Some white, most yellow parasites. -
Microorganisms – Protists: Euglena
Microorganisms – Protists: Euglena Euglena are unicellular organisms classified into the Kingdom Protista, and the Phylum Euglenophyta. All euglena have chloroplasts and can make their own food by photosynthesis. They are not completely autotrophic though, euglena can also absorb food from their environment. Euglena usually live in quiet ponds or puddles. Euglena move by a flagellum (plural flagella), which is a long whip-like structure that acts like a little motor. The flagellum is located on the anterior (front) end, and twirls in such a way as to pull the cell through the water. It is attached at an inward pocket called the reservoir. Color and label the reservoir grey. Color and label the flagellum black. The Euglena is unique in that it is both heterotrophic (must consume food) and autotrophic (can make its own food). Chloroplasts within the euglena trap sunlight that is used for photosynthesis and can be seen as several rod-like structures throughout the cell. Color and label the chloroplasts green. Euglena also have an eyespot at the anterior end that detects light, it can be seen near the reservoir. This helps the euglena find bright areas to gather sunlight to make their food. Color and label the eyespot red. Euglena can also gain nutrients by absorbing them across their cell membrane, hence they become heterotrophic when light is not available, and they cannot photosynthesize. The euglena has a stiff pellicle outside the cell membrane that helps it keep its shape, though the pellicle is somewhat flexible, and some euglena can be observed scrunching up and moving in an inchworm type fashion. -
Essential Function of the Alveolin Network in the Subpellicular
RESEARCH ARTICLE Essential function of the alveolin network in the subpellicular microtubules and conoid assembly in Toxoplasma gondii Nicolo` Tosetti1, Nicolas Dos Santos Pacheco1, Eloı¨se Bertiaux2, Bohumil Maco1, Lore` ne Bournonville2, Virginie Hamel2, Paul Guichard2, Dominique Soldati-Favre1* 1Department of Microbiology and Molecular Medicine, Faculty of Medicine, University of Geneva, Geneva, Switzerland; 2Department of Cell Biology, Sciences III, University of Geneva, Geneva, Switzerland Abstract The coccidian subgroup of Apicomplexa possesses an apical complex harboring a conoid, made of unique tubulin polymer fibers. This enigmatic organelle extrudes in extracellular invasive parasites and is associated to the apical polar ring (APR). The APR serves as microtubule- organizing center for the 22 subpellicular microtubules (SPMTs) that are linked to a patchwork of flattened vesicles, via an intricate network composed of alveolins. Here, we capitalize on ultrastructure expansion microscopy (U-ExM) to localize the Toxoplasma gondii Apical Cap protein 9 (AC9) and its partner AC10, identified by BioID, to the alveolin network and intercalated between the SPMTs. Parasites conditionally depleted in AC9 or AC10 replicate normally but are defective in microneme secretion and fail to invade and egress from infected cells. Electron microscopy revealed that the mature parasite mutants are conoidless, while U-ExM highlighted the disorganization of the SPMTs which likely results in the catastrophic loss of APR and conoid. Introduction *For correspondence: Toxoplasma gondii belongs to the phylum of Apicomplexa that groups numerous parasitic protozo- Dominique.Soldati-Favre@unige. ans causing severe diseases in humans and animals. As part of the superphylum of Alveolata, the ch Apicomplexa are characterized by the presence of the alveoli, which consist in small flattened single- membrane sacs, underlying the plasma membrane (PM) to form the inner membrane complex (IMC) Competing interest: See of the parasite. -
Introduction to Bacteriology and Bacterial Structure/Function
INTRODUCTION TO BACTERIOLOGY AND BACTERIAL STRUCTURE/FUNCTION LEARNING OBJECTIVES To describe historical landmarks of medical microbiology To describe Koch’s Postulates To describe the characteristic structures and chemical nature of cellular constituents that distinguish eukaryotic and prokaryotic cells To describe chemical, structural, and functional components of the bacterial cytoplasmic and outer membranes, cell wall and surface appendages To name the general structures, and polymers that make up bacterial cell walls To explain the differences between gram negative and gram positive cells To describe the chemical composition, function and serological classification as H antigen of bacterial flagella and how they differ from flagella of eucaryotic cells To describe the chemical composition and function of pili To explain the unique chemical composition of bacterial spores To list medically relevant bacteria that form spores To explain the function of spores in terms of chemical and heat resistance To describe characteristics of different types of membrane transport To describe the exact cellular location and serological classification as O antigen of Lipopolysaccharide (LPS) To explain how the structure of LPS confers antigenic specificity and toxicity To describe the exact cellular location of Lipid A To explain the term endotoxin in terms of its chemical composition and location in bacterial cells INTRODUCTION TO BACTERIOLOGY 1. Two main threads in the history of bacteriology: 1) the natural history of bacteria and 2) the contagious nature of infectious diseases, were united in the latter half of the 19th century. During that period many of the bacteria that cause human disease were identified and characterized. 2. Individual bacteria were first observed microscopically by Antony van Leeuwenhoek at the end of the 17th century. -
Protist Phylogeny and the High-Level Classification of Protozoa
Europ. J. Protistol. 39, 338–348 (2003) © Urban & Fischer Verlag http://www.urbanfischer.de/journals/ejp Protist phylogeny and the high-level classification of Protozoa Thomas Cavalier-Smith Department of Zoology, University of Oxford, South Parks Road, Oxford, OX1 3PS, UK; E-mail: [email protected] Received 1 September 2003; 29 September 2003. Accepted: 29 September 2003 Protist large-scale phylogeny is briefly reviewed and a revised higher classification of the kingdom Pro- tozoa into 11 phyla presented. Complementary gene fusions reveal a fundamental bifurcation among eu- karyotes between two major clades: the ancestrally uniciliate (often unicentriolar) unikonts and the an- cestrally biciliate bikonts, which undergo ciliary transformation by converting a younger anterior cilium into a dissimilar older posterior cilium. Unikonts comprise the ancestrally unikont protozoan phylum Amoebozoa and the opisthokonts (kingdom Animalia, phylum Choanozoa, their sisters or ancestors; and kingdom Fungi). They share a derived triple-gene fusion, absent from bikonts. Bikonts contrastingly share a derived gene fusion between dihydrofolate reductase and thymidylate synthase and include plants and all other protists, comprising the protozoan infrakingdoms Rhizaria [phyla Cercozoa and Re- taria (Radiozoa, Foraminifera)] and Excavata (phyla Loukozoa, Metamonada, Euglenozoa, Percolozoa), plus the kingdom Plantae [Viridaeplantae, Rhodophyta (sisters); Glaucophyta], the chromalveolate clade, and the protozoan phylum Apusozoa (Thecomonadea, Diphylleida). Chromalveolates comprise kingdom Chromista (Cryptista, Heterokonta, Haptophyta) and the protozoan infrakingdom Alveolata [phyla Cilio- phora and Miozoa (= Protalveolata, Dinozoa, Apicomplexa)], which diverged from a common ancestor that enslaved a red alga and evolved novel plastid protein-targeting machinery via the host rough ER and the enslaved algal plasma membrane (periplastid membrane). -
Amoeba, Paramoecium, Euglena and Diatom
BIOLOGY CLASSIFICATION OF PLANTS Kingdom Protista Introduction to Kingdom Protista Kingdom Protista consists of unicellular organisms. They contain a well-defined nucleus and a nucleolus enclosed in a nuclear membrane. The protoplasm is surrounded by the plasma membrane. The cytoplasm contains various cell organelles. Nuclear material is organised in the form of a linear, double-stranded and helical DNA, along with proteins. The mode of nutrition is either autotrophic or heterotrophic. Examples: Amoeba, Paramoecium, Euglena and diatom Amoeba Paramoecium Euglena Diatom Amoeba Amoeba is microscopic and one of the simplest organisms made of just one cell. It is found in ponds, ditches, mud or on submerged water plants in freshwater bodies. The body of Amoeba is irregular in shape. The outer membrane called cell membrane or plasmalemma encloses the cytoplasm. A prominent nucleus, several food vacuoles, a contractile vacuole and reserve food granules are present. Amoeba uses pseudopodia for feeding and locomotion. It reproduces by binary fission. During unfavourable conditions (e.g. drying up of pond), it forms a protective cyst within which it reproduces by multiple fission, producing several offspring. Structure of Amoeba www.topperlearning.com 2 BIOLOGY CLASSIFICATION OF PLANTS Movement in Amoeba Movement in Amoeba occurs with the help of temporary or false feet called pseudopodia. Pseudopodia are finger-like projections formed by the flowing of cytoplasm into these extensions. At a time, several pseudopodia can be seen projecting out from the body of an Amoeba. However, only one of them extends longer than the others towards the direction it wants to move. This type of movement is called amoeboid movement. -
Protozoa : Locomotion and Nutrition
Protozoa : locomotion and Nutrition Lecturer : P. V. Deokar Department of Zoology R.K.M.M. Ahmednagar Locomotion in Protozoa • The following points highlight the three main types of locomotion exhibited by protozoans. The types of locomotion are: • 1. Amoeboid Movement • 2. Flagellar Movement • 3. Ciliary Movement. Protozoans: Type of Locomotion # 1. Amoeboid Movement: • movement of the animal is made by the throwing of pseudopodium, called amoeboid movement • In the direction of movement of Amoeba a new pseudopodium is formed and the pseudopodium at the opposite side gradually disappears. • Types of pseudopodia: • According to form, structure and activity four different kinds of pseudopodia are recognised • These are: • (a) Lobopodium • (b) Filopodium • (c) Reticulopodium or Rhizopodium • (d) Axopodium or Actinopodium (a) Lobopodium [Gk. lobes = lobe; podium = foot]: • It is a short, finger or tongue-like projection which is accompanied by a flow of endoplasm and ectoplasm. • The pseudopodium is broad with rounded or blunt tips. • The ectoplasmmic area is distinctly clear, called the hyaline cap. • It is the characteristic of many amoebas such as Amoeba. (b) Filopodium [L.filo = a thread; podium = foot]: • The filopodium is a slender, thread-like or filamentous projection. • It is formed by the ectoplasm alone and without a hyaline cap. • The filaments are narrow and may be branched but do not anastomose, Filopodium is the characteristic in Filosea (e.g., Euglypha ). (c) Reticulopodium or Rhizopodium [L. reticulos = a net, podium = foot]: • Similar in structure to that of filopodium but the branches anastomose. • The numerous branched and anastomosed pseudopodia form a dense network, help primarily in capturing the prey and the secondary function is locomotion. -
Brown Algae and 4) the Oomycetes (Water Molds)
Protista Classification Excavata The kingdom Protista (in the five kingdom system) contains mostly unicellular eukaryotes. This taxonomic grouping is polyphyletic and based only Alveolates on cellular structure and life styles not on any molecular evidence. Using molecular biology and detailed comparison of cell structure, scientists are now beginning to see evolutionary SAR Stramenopila history in the protists. The ongoing changes in the protest phylogeny are rapidly changing with each new piece of evidence. The following classification suggests 4 “supergroups” within the Rhizaria original Protista kingdom and the taxonomy is still being worked out. This lab is looking at one current hypothesis shown on the right. Some of the organisms are grouped together because Archaeplastida of very strong support and others are controversial. It is important to focus on the characteristics of each clade which explains why they are grouped together. This lab will only look at the groups that Amoebozoans were once included in the Protista kingdom and the other groups (higher plants, fungi, and animals) will be Unikonta examined in future labs. Opisthokonts Protista Classification Excavata Starting with the four “Supergroups”, we will divide the rest into different levels called clades. A Clade is defined as a group of Alveolates biological taxa (as species) that includes all descendants of one common ancestor. Too simplify this process, we have included a cladogram we will be using throughout the SAR Stramenopila course. We will divide or expand parts of the cladogram to emphasize evolutionary relationships. For the protists, we will divide Rhizaria the supergroups into smaller clades assigning them artificial numbers (clade1, clade2, clade3) to establish a grouping at a specific level.