Fast Manual Microplate Pipetting!
USER'S GUIDE Table of Contents 1 INTRODUCTION 3 User’s Guide and Safety 4-5 Unpacking 5 2 DESCRIPTION 6 Introduction 6 PLATEMASTER - General view 7 3 INSTALLATION 8 4 OPERATION 9 Unlocking the pipetting head 9 Volume setting 9 Tip fitting 10 Pipetting 10-11 Tip ejection 12 General guidelines for good pipetting 12 5 ACCESSORIES 13 Height adjuster 13 Pipetting 384 wells 13 Warranty Gilson warrants this product against defects in material 6 SERVICE 14 under normal use and service for a period of 12 months GLP features 14 from the date of purchase. Cleaning and decontamination 14 Specifications 14-15 Gilson is not responsible for incidental damage resulting from physical shock, continuous exposure to corrosive 7 APPENDICES 16 reagents, or technical handling not in accordance with Technical Data 16 the guidelines described in the User’s Guide. Warranty 18
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1 - INTRODUCTION
Gilson’s PLATEMASTER is a 96-channel manual pipetting system designed for laboratory applications using microplates. It can transfer liquids with high throughput, accuracy, and precision.
PLATEMASTER is ideal for high throughput applications as it will pipette 96 wells simultaneously and still be easy to use, based on the same working principle as a standard air-displacement pipette.
PLATEMASTER is suitable for a wide range of applications: • Pipetting and providing master mixes for PCR/qPCR. • Simultaneous processing of nearly all reaction steps in microtiter plates (coating, starting, stopping, washing, etc.) for applications like ELISA. • Addition and removal of culture media, plate replication in cell culture. • Dropwise addition of solutions for crystallography. • Plate replication in expression tests. • Enhancement of existing automation: assay development and adaptation to 96-channel systems, back-up solution for maintenance and downtime. • Whenever workflows involve pipetting on a 96-well or 384-well scale.
Gilson PLATEMASTER P20
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User’s Guide
Before using the unit, the user must know how to set up and operate the PLATEMASTER. Therefore, please make sure you read Chapters 1 to 3, including the safety notes. These chapters also describe some of the methodology required for obtaining accurate and reproducible results.
Chapter 1 - Introduction contains general information including the safety notes which should be read before installing and operating the device.
Chapter 2 - Description contains a detailed description of the PLATEMASTER.
Chapter 3 - Operation describes how to operate the PLATEMASTER.
Chapter 4 - Appendices contains details on accessories and technical data (specifications).
For further information visit www.gilson.com
Read this section carefully before installing and operating PLATEMASTER.
The instrument described in this User’s Guide is a manual pipetting system to be operated by qualified personnel in a laboratory or similar indoor environment. Cleaning, installation, dismantling, maintenance, adjustment and repair should be performed by appropriately trained aware of the hazards involved. An efficient ground connection is mandatory for the physical protection of the user. Adequate protection, such as ventilation, must be provided if dangerous liquids are used in analytical work. In case of incidental spillage, carefully clean up the spillage, consider the nature of the spilled liquid, and take all required safety measures, accordingly.
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Safety PLATEMASTER is designed for research purposes only. If PLATEMASTER is used in a manner not specified by Gilson, the protection provided by the instrument may be impaired. Gilson will not be responsible for consequential damages. The use of the transportation safety lock is only dedicated for the in house transport. For all other transportation we recommend to disassemble the pipetting head from the base unit and use the transportation box. Temperature of use: 4 to 40°C - Recommended working temperature: 20 to 25 °C Only use accessories provided. Gilson SAS denies any responsibility for service and repairs carried out by the customer or third- party companies. Neither the equipment nor the filter tips applied can be autoclaved. Gilson does not take liability for any damage resulting from the use of PLATEMASTER. Follow the instructions of this User’s Guide to ensure durability and top performance of your PLATEMASTER. Keep this manual handy for future reference, in accordance with Good Laboratory Practices. Pipetting aggressive liquids may damage PLATEMASTER parts in contact with the solution. In case of contact, immediately clean up aggressive liquids. Pipetting of extremely viscous or highly evaporating liquids is at your own risk. The same applies to aggressive and corrosive reagents. Avoid aspiration of any liquid into the tip-holders. Avoid handling under the pipetting head.
Unpacking
Each complete PLATEMASTER is shipped in one box. - Reference F110761 contains: - Reference F110762 contains: • PLATEMASTER P20 • PLATEMASTER P220 • User’s guide • User’s guide • 3 height adjusters • 3 height adjusters • 1 lubrication box • 1 lubrication box • 1 Allen ball head wrench 3.0 x 100 (for • 1 Allen ball head wrench 3.0 x 100 (for assembling and disassembling of the assembling and disassembling of the pipetting head) pipetting head) • 1 transport box and packing material • 1 transport box and packing material • Gilson original certificate of conformity • Gilson original certificate of conformity
- 5 - Upon receipt of your PLATEMASTER, unpack the items and check for completness, even if the device will not be used immediately. It is highly recommended to keep the packaging. If the unit has to be sent back to the factory, the original packaging must be used to return equipment under warranty. Report any loss or damage immediately.
2 - DESCRIPTION
Introduction
The pipetting head moves both vertically and horizontally. Vertical movements are performed for fitting tips and pipetting liquid, horizontal moves are performed for transferring liquid. This movement is possible only when the head is located completely at the top.
Two drawers can hold up to three different microplates and can be used at the same time.
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PLATEMASTER - General view
The figure below shows a general view of a PLATEMASTER.
Lock/Unlock Pipetting head Volume adjustment wheel Volume display Tip fitting handle Pipetting handle Tip-ejector Movable drawers with microplates and tip rack locations Pipetting head height adjuster
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1 Remove PLATEMASTER pipetting head and PLATEMASTER base from box.
Insert the pipetting head has follow and secure the head by screwing the first screw down.
Unlock the pipetting head, returning it to the upper position. Secure the head by fastening the screw located below the head.
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4 - OPERATION
Unlocking the pipetting head
Whether placed on the lab bench, under a fume hood, in a cold room, or used in the field, the PLATEMASTER is completely portable. The PLATEMASTER pipetting head includes a locking device to lock the head when the instrument being moved. After each use of the PLATEMASTER, remember to lock the pipetting head to prepare for transport.
To unlock the pipetting head, pull the black part on the right side of the PLATEMASTER and turn it in your direction. Once the pipetting head is released, check proper movement of the device.
To lock the pipetting head, pull the black part and turn it in the opposite direction, make sure that the pipetting head cannot move anymore.
PLATEMASTER is now securely locked. Never move the device without locking the pipetting head. Damages caused by movement without locking the device will not be covered by the warranty.
Volume setting
Do not adjust the volume without tightening both parts of the pipetting handle . The volume of liquid to be aspirated is set using the volume adjustment wheel .
The pipetting handle being tightened, the volume is set by turning the button slowly to reach the required setting.
Never try to force the volume outside of the volume range permitted by the device.
- 9 - Tip fitting
For optimum performance, use of PIPETMAN DIAMOND Tips with PLATEMASTER is strongly recommended. 5 These tips have the Gilson logo engraved on their 3 collar, ensuring that you have a genuine Gilson product. Plastic tips are for a single use – they must not be cleaned for reuse. 1
To fit PIPETMAN DIAMOND Tips on PLATEMASTER: 4 • Unlock the pipetting head and place it in the upper position. Place the pipetting head to the left side. 2 • Pull the right drawer and place a new rack of tips on the upper location. The four locations have been specially designed for PIPETMAN DIAMOND Tips TIPACK. The rack will perfectly fit into all the locations and will be stable due to the slots. • Move the pipetting head by using the pipetting handle to the right and move it down far enough so that a contact is made to the tips in the rack. • Press the tip fitting handle with moderate pressure down. The correct grabbing of the tips is indicated by the complete unfolding of the tip-ejector . • Move the pipetting head up and the right drawer back.
Check the tip-ejector when fitting tips. If the tips have shifted this implies that they have not been fitted correctly.
Pipetting
The device is now ready to pipetting liquid.
Place a vessel containing the solution to be pipetted in the front location of the right drawer. The solution should be under the pipetting head.
Liquids (e.g. protein-containing solutions and organic solvents) can leave a film of liquid on the inside wall of the tip ; pre-rinse the tip to minimize any errors related to this phenomenon.
Pre-rinsing consists of aspirating the first volume of liquid and then dispensing it back into the same vessel (or to waste). Subsequent volumes pipetted will have levels of accuracy and precision within specifications. As recommended by ISO 8655 standard, pre-rinse to stabilize dead volume inside the pipetting head.
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Aspiration • Smoothly tighten the pipetting handle to the first stop (this corresponds to the set volume of liquid). • Move the pipetting head down by using the pipet- ting handle to immerse the tips into the liquid (see immersion depth table below). It is important that the tips do not touch the ground. • Slowly open the pipetting handle to aspirate the liquid into the tips. • Move the pipetting head fully up. To simplify this operation, a height adjuster can be set so that the pipetting head may only be moved up to a position slightly above the bottom.
Table - Immersion Depth and Wait Time Model Immersion Wait Time Depth (mm) (seconds) The maximum immersion depth of the tips can be adjusted P20 2-3 1 using a height adjuster P220 2-4 1
Dispensing
Place a 96-well microplate at the front location of the left drawer and keep it fully pushed into the rear position. • Move the pipetting head to the left and align the tips with the microplate. • Pull the pipetting head slowly down closer to the wells without touching the bottom of the wells. • Tighten the pipetting handle slowly up to the first stop to dispense the solution. • Wait for at least one second, then tighten the pipet- ting handle up to the second stop to expel any residual liquid from the tip. • Pull the pipetting head slowly up with tightened pipetting handle. • Release the pipetting handle smoothly. To simplify this operation, a height adjuster 4 can be set so that the pipetting head may only be moved up to a position slightly above the bottom.
- 11 - Tip ejection
• Move the pipetting head over the empty rack on the right drawer. • Pull the pipetting head down to put the used tips into the rack. • Squeeze the tip-ejector at the top of the pipetting head to eject the tips into the rack.
General guidelines for good pipetting
Place the PLATEMASTER in a dry and clean environment at a recommended ambient temperature of 20 to 25 °C. These conditions should stay constant, as significant variations in temperature or humidity may affect precision.
PLATEMASTER is completely portable and requires no electricity, it can be operated everywhere in the lab. When the device has been moved, allow some equilibration time for the instrument to adjust to the new ambient conditions.
Make sure that you operate the pipetting handle slowly and smoothly. Aspirating too quickly may cause drops to splash inside the tips . Dispensing too quickly can cause carry-over. Pipetting speed will depend on the type of liquid used.
Never let any liquid enter into the tip-holders. This phenomenon can be prevented by tightening and releasing the pipetting handle slowly and smoothly.
Change the tips before aspirating a different liquid, sample, or reagent.
Each new tip should be pre-rinsed with the liquid to be pipetted.
Do not pipette liquids having temperatures above 70 °C or below 4 °C. The PLATEMASTER can be used between + 4 °C and + 40 °C but specifications may vary according to temperature (refer to the ISO 8655-6 standard for conditions of use).
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5 - ACCESSORIES
Height adjuster
The descent of the pipetting head and the position of the tips can be adjusted by means of 3 height adjusters . This will avoid the tips to touch the bottom of the microplate. Whether the microplate has long or short wells, the descent of the pipetting head can be adjusted to the microplate.
Pipetting 384 wells
PLATEMASTER is able to load 384-well plates through four PLATEMASTER moves using the 384-well plate adapter.
• Place a 384-well microplate on the accessory and make sure that it is located at the 1st step. • Aspirate the solution. • Move the pipetting head over the microplate and dispense the first 96 wells.
• Move the microplate on the 2nd position of the 384-well plate adapter. • Aspirate the solution. Move the pipetting head over the microplate and dispense the next 96 wells.
• Move the microplate on the 3rd position of the 384-well plate adapter. • Aspirate the solution. Move the pipetting head over the microplate and dispense 96 wells.
• Move the microplate on the 4th position of the 384-well plates adapter. • Aspirate the solution. Move the pipetting head over the microplate and dispense the last 96 wells.
The 384-well microplate is loaded in less than 1 minute requiring only 4 liquid aspiration steps.
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GLP Features
The serial number is engraved on the back of the pipetting head. It provides unique identification of your PLATEMASTER and the date of manufacture.
The certificate of conformity provides traceability of your PLATEMASTER.
Cleaning and decontamination
Do not use corrosive and acid reagents to clean and decontaminate PLATEMASTER.
PLATEMASTER must be cleaned before it is decontaminated. Wipe the surface of the instrument with alcohol or laboratory disinfectant.
The PLATEMASTER surface can be cleaned with a soft tissue moistened with water or laboratory disinfectant.
Do not expose PLATEMASTER to excessive UV radiation and never overnight. Do not autoclave any part of PLATEMASTER.
Use of the lubrication box
In case of intensive use between each maintenance interval and to avoid bad tip fitting, it is recommended to lightly lubricate the external O-ring using the lubrication box of O-ring part F1077606.
Proceed as follow : • Open the box lid. • Follow instruction as described page 10 – Tip fitting. • Press the fitting handle until the correct tips grabbing. • Eject the tips.
The O-ring lubrication is realized. Using a no-fiber wipe, remove any exceed of lubricant on the pin-plate (see image)
Specifications
PLATEMASTER is a high quality 96-channel manual pipetting system that offers excellent accuracy and precision. The figures given in the “Gilson maximum permissible errors” table below were obtained using PIPETMAN DIAMOND Tips. These figures are guaranteed only when genuine PIPETMAN DIAMOND Tips are used.
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P20 is certified with D200. P220 is certified with D300.
Each PLATEMASTER is inspected and validated by qualified technicians in accordance with the Gilson Quality System.
The adjustment is carried out under strictly defined and monitored conditions (ISO 8655-6).
Gilson maximum perimissible errors
PLATEMASTER P20 Volume range from 0.5 to 20 µL Volume Maximum Permissible Errors µL Gilson ISO 8655 Systematic (µL) Random (µL) Systematic (µL) Random (µL)
1 ± 0.12 ≤ 0.1 ± 0.4 ≤ 0.20
10 ± 0.12 ≤ 0.1 ± 0.4 ≤ 0.20
20 ± 0.2 ≤ 0.18 ± 0.4 ≤ 0.20
PLATEMASTER P220 Volume range from 2 to 220 µL Volume Maximum Permissible Errors µL Gilson ISO 8655 Systematic (µL) Random (µL) Systematic (µL) Random (µL)
2 ± 0.12 ≤ 0.15 ± 8.0 ≤ 3.0
5 ± 0.25 ≤ 0.175 ± 8.0 ≤ 3.0
20 ± 0.4 ≤ 0.3 ± 8.0 ≤ 3.0
100 ± 1 ≤ 0.6 ± 8.0 ≤ 3.0
200 ± 1.6 ≤ 0.8 ± 8.0 ≤ 3.0
220 ± 1.8 ≤ 0.8 ± 8.0 ≤ 3.0
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Technical data
Model PLATEMASTER P20 PLATEMASTER P220
Ordering Reference F110761 F110762 Volume Range 0.5 µL - 20 µL 2 µL to 220 µL Volume can be adjusted Volume can be adjusted in 0.1 µL increments in 1 µL increments Temperature Range Recommended working temperature : 20 to 25 °C Dimensions 300 x 450 x 400 mm (W x L x H) Weight approx. 16 kg
Accessories Part Number
Adapter 384-well. Positioning done by wheel. F1077602
Adapter 384-well. Positioning done by hand. F1077603
Alu-heater block for PLATEMASTER, 96 x 0.2 mL, F1077604 for PCR tubes, stripes & PCR plates ∅ 7.8 mm
Pipetting head height adjuster F1077605
Lubrication box of O-rings PLATEMASTER F1077606
F1077602 F1077604
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Validated for use with Tip model Standard γ-ray sterilized 384-well plate Filter tips PLATEMASTER P20 D200 D200ST DS200 DS200ST DSF200 DF200 PLATEMASTER P220 D200 D200ST DF200 DS200 DS200ST DSF200 D300 D300ST DF300
All used consumables are tested on the specific volume ranges of PLATEMASTER.
- 17 - WARRANTY
Gilson warrants this device against defects in material under normal use and service for a period of 12 months from the date of purchase. This warranty shall not apply to devices which are subject to abnormal use and/or improper or inadequate maintenance (contrary to the recommendations given in the User’s guide), including, but not limited to devices which have been subjected to physical damage, improper handling, spillage or exposure to any corrosive environment. This warranty shall also be void in the event devices are altered or modified by any party other than Gilson or its designates. Gilson’s sole liability under this warranty shall be limited to, at Gilson’s sole option, repair or replacement of any defective components of devices or refund of the purchase price paid for such devices. THE FOREGOING WARRANTY IS EXCLUSIVE AND GILSON HEREBY DISCLAIMS ALL OTHER WARRANTIES, WHETHER EXPRESS OR IMPLIED, INCLUDING ANY WARRANTIES OF MERCHANTABILITY AND ANY WARRANTIES OF FITNESS FOR A PARTICULAR PURPOSE, UNDER NO CIRCUMSTANCES SHALL GILSON BE LIABLE FOR ANY CONSEQUENTIAL, PUNITIVE, INDIRECT OR INCIDENTAL DAMAGES ARISING OUT OF ANY BREACH OF ANY EXPRESS OR IMPLIED WARRANTY.
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- 19 - LT801549/B - ©2015 Gilson SAS All rights reserved February 2015 English Specifications subject to change without notifications - errors excepted. Reproduction, adaptation, or translation without prior written permission is prohibited, except as allowed under copyright laws. www.gilson.com [email protected] [email protected] [email protected]
Gilson, Inc. World Headquarters 3000 Parmenter Street I P.O. Box 628098 I Middleton, WI 53562, USA Tel: (1) 800-445-7661 I Fax: (1) 608-821-4403 Gilson S.A.S. 19, avenue des Entrepreneurs I BP 145, F-95400 Villiers-le-Bel, France Tel: +33 (0) 1 34 29 50 00 I Fax: +33 (0) 1 34 29 50 20