Bravo Automated Liquid-Handling Platform User Guide Iii Contents
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Bravo Automated Liquid-Handling Platform User Guide Original Instructions Agilent Technologies Notices © Agilent Technologies, Inc. 2012 Warranty (June1987) or DFAR 252.227-7015 (b)(2) (November 1995), as applicable in any No part of this manual may be reproduced The material contained in this docu- technical data. in any form or by any means (including ment is provided “as is,” and is sub- electronic storage and retrieval or ject to being changed, without notice, Safety Noticies translation into a foreign language) without prior agreement and written consent from in future editions. Further, to the max- A WARNING notice denotes a Agilent Technologies, Inc. as governed by imum extent permitted by applicable hazard. It calls attention to an United States and international copyright law, Agilent disclaims all warranties, operating procedure, practice, or the laws. either express or implied, with regard like that, if not correctly performed or to this manual and any information User Guide Part Number adhered to, could result in personal contained herein, including but not injury or death. Do not proceed G5409-90006 limited to the implied warranties of beyond a WARNING notice until the merchantability and fitness for a par- indicated conditions are fully Edition ticular purpose. Agilent shall not be understood and met. liable for errors or for incidental or Revision 03, September 2012 consequential damages in connection A CAUTION notice denotes a hazard. It Contact Information with the furnishing, use, or perfor- calls attention to an operating procedure, mance of this document or of any practice, or the like that, if not correctly performed or adhered to, could result in Agilent Technologies Inc. information contained herein. Should Automation Solutions damage to the product or loss of important Agilent and the user have a separate 5301 Stevens Creek Blvd. data. Do not proceed beyond a CAUTION Santa Clara, CA 95051 written agreement with warranty notice until the indicated conditions are USA terms covering the material in this fully understood and met. document that conflict with these Technical Support: 1.800.979.4811 (US only) terms, the warranty terms in the sep- or +1.408.345.8011 (rest of the world) arate agreement shall control. [email protected] Customer Service: 1.866.428.9811 Technology Licenses or +1.408.345.8356 The hardware and/or software described in [email protected] this document are furnished under a European Service: +44 (0)8457125292 license and may be used or copied only in [email protected] accordance with the terms of such license. Documentation feedback: Restricted Rights Legend [email protected] If software is for use in the performance of Web: a U.S. Government prime contract or www.agilent.com/lifesciences/ subcontract, Software is delivered and automation licensed as “Commercial computer software” as defined in DFAR 252.227-7014 Acknowledgements (June 1995), or as a “commercial item” as Adobe® and Acrobat® are trademarks of defined in FAR 2.101(a) or as “Restricted Adobe Systems Incorporated. computer software” as defined in FAR 52.227-19 (June 1987) or any equivalent Microsoft® and Windows® are either agency regulation or contract clause. Use, registered trademarks or trademarks of the duplication or disclosure of Software is Microsoft Corporation in the United States subject to Agilent Technologies’ standard and other countries. commercial license terms, and non-DOD Departments and Agencies of the U.S. Government will receive no greater than Restricted Rights as defined in FAR 52.227- 19(c)(1-2) (June 1987). U.S. Government users will receive no greater than Limited Rights as defined in FAR 52.227-14 Contents Preface . vii About this guide . viii Accessing Automation Solutions user information. ix 1. Introduction to the Bravo Platform . 1 Safety notes . 2 About the Bravo Platform . 3 Hardware overview. 5 Indicator lights. 10 Connection panel description . 11 Pipette heads and pin tools . 13 Software overview . 16 2. Setting up the Bravo Platform . 19 Workflow for setting up the Bravo Platform. 20 Starting up and shutting down . 21 Creating or adding a Bravo device . 23 Opening Bravo Diagnostics . 28 Creating and managing profiles . 30 Initializing the Bravo Platform. 34 Setting teachpoints. 36 Verifying the gripper setup . 44 Configuring external robot access . 47 3. Preparing for a protocol run . 57 Workflow for preparing a protocol run. 58 Emergency stops. 58 Planning for the protocol run. 60 About performing dry runs. 60 4. Maintaining the Bravo Platform . 63 Routine maintenance . 64 Cleaning the Bravo Platform . 65 Cleaning up after a run. 66 Changing the Bravo head. 67 Replacing the fuse . 73 Retracting tip box stripper pins. 74 Bravo Automated Liquid-Handling Platform User Guide iii Contents Moving the head manually . 76 Recovering from a head collision. 77 Troubleshooting hardware problems . 79 Troubleshooting hardware-related error messages . 81 Reporting problems . 82 5. Using Bravo Diagnostics. 85 About the Jog/Teach tab . 86 Homing the pipette head. 88 Jogging the pipette head. ..