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Stimulation of Stevioside Accumulation on Stevia Rebaudiana

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Stimulation of Stevioside Accumulation on Stevia Rebaudiana World Academy of Science, Engineering and Technology International Journal of Biotechnology and Bioengineering Vol:10, No:12, 2016 6WLPXODWLRQRI6WHYLRVLGH$FFXPXODWLRQRQStevia rebaudiana %HUWRQL 6KRRW&XOWXUH,QGXFHGZLWK 5HG/('/LJKWLQ7,65,7$%LRUHDFWRU6\VWHP 9LQFHQW$OH[DQGHU5L]NLWD(V\DQWL VWHYLDZKLFKPDLQO\FRPSULVHGRIVWHYLRVLGHDQGUHEDXGLRVLGH Abstract²/HDYHVRIStevia rebaudianaFRQWDLQVWHYLROJO\FRVLGH $ %RWK FRPSRXQGV DUH DSSURYHG E\ )$2 DQG %3320 RI ZKLFKPDLQO\FRPSULVHRIVWHYLRVLGHDQDWXUDOVZHHWHQHUFRPSRXQG ,QGRQHVLDWRVXEVWLWXWHVXJDUVZHHWHQHULQIRRGDQGEHYHUDJH WKDW LV WLPHV VZHHWHU WKDQ VXFURVH &XUUHQW FXOWLYDWLRQ SURGXFWV WKXV PDNLQJ LW VDIH IRU SHRSOH ZLWK GLDEHWLF LVVXH PHWKRG RI Stevia rebaudiana LQ ,QGRQHVLD KDV \HW WR UHDFK LWV >@ RSWLPXPHIILFLHQF\DQGSURGXFWLYLW\WRSURGXFHVWHYLRVLGHDVDVDIH VXJDU VXEVWLWXWH VZHHWHQHU IRU SHRSOH ZLWK GLDEHWHV $Q DOWHUQDWLYH Stevia rebaudiana LV FRPPRQO\ FXOWLYDWHG XVLQJ in vivo PHWKRG WKDW LV QRW 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RQ VWHYLRVLGH FRQWHQW LQ VWHYLD GLDEHWLFHSLGHPLF>@6WHYLD Stevia rebaudiana%HUWRQL LVDQ SURSDJDWHG E\ in vitro VKRRW FXOWXUH &RUUHVSRQGLQJO\ WKLV $VWHUDFHDH IDPLO\ SODQW IURP WKH KLJK JURXQGV RI 6RXWKHUQ International Science Index, Biotechnology and Bioengineering Vol:10, No:12, 2016 waset.org/Publication/10005871 UHVHDUFK DLPV WR HYDOXDWH WKH UHVSRQVH RI UHG /(' OLJKW $PHULFD ZLWK SDUWLFXODU FRQWHQWV RI QDWXUDO VZHHWHQHU LQ LWV VWLPXODWLRQ RQ VKRRWV RI VWHYLD FXOWXUHG LQ 5,7$ELRUHDFWRU OHDYHV ZKLFK LV HVWLPDWHG WR EH WLPHV VZHHWHU WKDQ V\VWHPWRZDUGVLWVVWHYLRVLGHDFFXPXODWLRQELRPDVVFKDQJHV VXFURVH ZLWK YLUWXDOO\ ]HUR FDORULHV >@ 7KH FRPSRXQG JURZWKUDWHDQGQXWULWLRQUDWHRILQWDNHE\ELRPDVVRIStevia UHVSRQVLEOH LV D GLWHUSHQH JO\FRVLGH VHFRQGDU\ PHWDEROLWH RI rebaudiana GXULQJSHULRGRIFXOWLYDWLRQ 9LQFHQW $OH[DQGHU LV VWXGHQW LQ %LRORJLFDO (QJLQHHULQJ 0DMRU %DQGXQJ ,QVWLWXWH RI 7HFKQRORJ\ %DQGXQJ ,QGRQHVLD HPDLO YLQFHDOH[DQGHUU#VWXGHQWVLWEDFLG 5L]NLWD (V\DQWL LV OHFWXUHU LQ 6FKRRO RI /LIH 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