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Animal Science/Dairy Science 434 Reproductive Tract

Reproductive Physiology Female Anatomy

John Parrish, Professor, UW-Madison

Learning Objectives Equine - Horse

• To be able to describe the female Male Stallion Female Mare in cattle. »Structure »Function

Updated:9/6/17

Bovine - Cattle Ovine - Sheep Male Bull Female Cow Male Ram Female Ewe

Updated:9/6/17 Updated:9/6/17 Avian - Chicken Porcine - Swine - Pig Male Rooster Female Hen Male Boar Female Sow

Updated:9/6/17 Updated:9/6/17

Uterine Uterine Horn Uterine Horn Rectum Ovary Oviduct

Vulva Oviduct Ovary Bladder

Vagina Bladder Cervix Oviduct

Urethra Broad Ligamen t

Updated:9/6/17 Updated:9/6/17

Broad Broad Ligament • » Supports Uterus • • connective tissue » Supports Oviduct sheet which • supports and » Supports Ovary suspends the reproductive tract BL - Broad Ligament UH - Uterine Horn O - Ovary OD - Oviduct Updated:9/6/17 Ovary Ovary - Primary Follicle - , Oocyte • Functions »Hormonal • Estrogen • »Female • Oocyte Corpus Luteum Progesterone Progesterone

Updated:9/6/17 Updated:9/6/17

Ruptured follicle Tertiary follicle Ovary (ovulation) Primary follicle Germinal Egg nest Epithelium Cortex 85% of Ovarian Tertiary Cancers follicle

Medulla Atretic follicle Secondary Tunica follicle Albuginea Blood and lymphatic Vessels & nerves Hilus } Corpus Corpus luteum hemorrhagicum Updated:9/6/17 Updated:9/6/17

Ruptured follicle Tertiary follicle Ruptured follicle Tertiary follicle (ovulation) Primary follicle (ovulation) Primary follicle

Tertiary Tertiary follicle follicle

Atretic follicle Atretic follicle Secondary Secondary follicle follicle

Corpus Corpus albicans Corpus luteum Corpus Corpus luteum Corpus albicans hemorrhagicum hemorrhagicum Updated:9/6/17 Updated:9/6/17 Ruptured follicle Tertiary follicle Follicle Cells Primary follicle (ovulation) Primordial Follicle Nucleus • Flattened cells surround oocyte Tertiary follicle Primary Follicle Ooplasma Increase in size of oocyte and • Follicular Cells height of follicular cells

Atretic follicle Secondary follicle Oocyte Secondary • Surrounded by several follicle layers of follicular cells Tertiary follicle • Fluid filled cavity Corpus Corpus albicans Corpus luteum • Differentiation of cell layers hemorrhagicum Antrum Updated:9/6/17 in the follicle wall

Tertiary Follicle Tertiary Follicle

Theca Externa Antrum () Theca Interna Granulosa Basement Membrane Oocyte Cumulus Granulosa Oophorus

Oocyte Antrum

Updated:9/6/17 Updated:9/6/17

Follicular Blood Supply Secondary : A Series of Tubes to Convey the Theca interna cells Oocyte/Embryo/ to the Outside Basement Membrane

Granulosa cells Serosa

Capillary Follicular network fluid Submucosa

Lumen Longitudinal smooth muscle layer Circular smooth Mucosa muscle layer Muscularis

Updated:9/6/17 Updated:9/6/17 Uterine Horn Functions of Oviduct Isthmus • Gamete Transport Utero-Tubal Junction »Sperm Oviduct »Oocyte Ampullary-Isthmic Junction • Supports early Ampulla embryo development Infundibulum (20 - 30 cm2) • Transport of embryo Ostium to uterus Cumulus Oophorus and Oocyte Fimbria

Ovary Follicle Updated:9/6/17 Updated:9/6/17

Oviduct Oviduct

Oviduct Histology Functions of the Uterus • Muscle contractions Mucosa » Transport sperm •Ciliated Columnar » Expel fetus and placenta •Nonciliated Columnar • Absorption and phagocytosis • Partially prepares sperm for fertilization » capacitation • Provides environment for embryo and fetal growth • Hormone production » Prostaglandin F2a production

Updated:9/6/17 Updated:9/6/17 Uterus (bovine) horn Uterus

• Bicornuate body » poorly to moderately developed cervix » uterine body » one cervix

Updated:9/6/17

Uterine Histology Uterine Histology Mucosa

Endometrial Endometrium Gland Submucosa

Myometrium

Endometrial Perimetrium Updated:9/6/17 Glands Updated:9/6/17

Functions of the Cervix Uterine Body COW • Cervix is Internal Cervical Os • Transport of sperm composed of thick connective tissue • Barrier to sperm (species dependent) • Mucus is secreted • Reservoir for sperm near the time of Cervix breeding and • Blocking bacterial invasion during pregnancy Cow has 4-5 ovulation. • Birth canal annular rings

External Cervical Os

Updated:9/6/17 Updated:9/6/17 Vagina External Cervical Os

Functions of the Vagina Vagina Anterior Vagina Posterior Vagina (Vestibule) Cervix Copulatory organ • Fornix • Glands secrete lubrication Vulva- • Birth canal Vaginal Sphincter Stratified • Glands secrete pheromones Columnar Squamous Epithelium Epithelium

Mucosa

Submucosa Submucosa

Updated:9/6/17 Updated:9/6/17

Posterior Vaginal Histology Function of External Genitalia • »Closes entrance to vagina Stratified Squamous Epithelium • Clitoris »Female sensory organ

Mucosa

Submucosa Updated:9/6/17 Updated:9/6/17 External Genitalia of the Cow Phenomena For Fertility • vagina dentata - a vagina with teeth Anus • female bedbug - no vagina Vulva • - if went to long Labia without becoming pregnant then uterus would wonder in body causing hysteria and other distresses Clitoris • hyena - clitoris is very large and looks like the male ; female also has a false Labia (labia); female is the dominate sex and produces more than male

Updated:9/6/17 Updated:9/6/17