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Pet Food Microbiology We Are Here for You, Like You Are for Them

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Pet Food Microbiology We Are Here for You, Like You Are for Them Pet Food Microbiology We are here for you, like you are for them. Contents Pathogen Detection 2 E. coli 3 Listeria spp. 4 Salmonella spp. 5 General Culture Media 7 Control Organisms 8 CompactDry™ Dry Media Plates 10 The Wizard™ CompactDry™ Plate Reader 11 Sample Preparation 12 Serial Dilution 18 Rapid Tests Enviromental Monitoring 20 Surface Sampling 24 Trio.Bas™ Air Samplers Lab Supplies 25 Durarack™, Gloves, Lab Coats PATHOGEN DETECTION Escherichia coli Media MacConkey Agar with Sorbitol Indole Nitrate Broth (SMAC) For the detection of nitrate reduction and For the detection of enterohemorrhagic indole production in bacteria. Contains a E. coli O157 (sorbitol negative). durham tube for gas detection. 15x100mm plate, 10/pk G36 15x103mm tube, 20/pk K147 HardyCHROM™ O157 MacConkey Broth, USP A selective and differential medium Used for cultivating gram-negative, recommended for the isolation of lactose-fermenting bacilli and as a enterohemorrhagic E. coli O157 from food presumptive test for coliform organisms. and environmental sources. 180ml PC Bottle, 12/pk 5 U12 15x100mm plate, 10/pk G305 SHIBAM Agar Used as a selective and differential growth HardyCHROM™ ECC A chromogenic media recommended medium for the cultivation of Shiga toxin for the detection, differentiation, and producing E. coli (STEC). enumeration of E. coli and other coliforms 15x100mm plate, 10/pk A146 in food, water, or environmental samples based on colony color. CT-SMAC with Cefixime and 15x100mm plate, 10/pk G303 Tellurite (MacConkey with Sorbitol, Tellurite and EC Broth with MUG Cefixime) For selection and differentiation For the detection of E. coli in food of enterohemorrhagic E. coli O157. and water by flourescence. Contains 15x100mm plate, 10/pk G129 methylumbelliferyl glucuronide. 16x125mm tube, 20/pk K64 CompactDry™ EC E. coli and Coliforms For the isolation and enumeration of coliforms and E. coli. Coliform colonies will appear red, and E. coli colonies will appear blue. 240/pk 54082 Stx (Shiga-Toxin) Induction Broth Recommended as an enrichment method for the stimulation of Shiga-toxin (Stx) production by Shiga toxin-producing E. coli (STEC). 15x103mm tube, 20/pk K274 2 Cat. no. A146 Listeria spp. Media Buffered Listeria Enrichment HardyCHROM™ Listeria Broth Base, BAM HardyCHROM™ Listeria is a chromogenic Buffered Listeria Enrichment Broth Base medium recommended for the selective is used in procedures for the isolation isolation, differentiation, and enumeration of Listeria monocytogenes from food of Listeria monocytogenes from food and samples. environmental samples by colony color and appearance. 500ml wide mouth jar, 12/pk U233 15x100mm plate, 10/pk G317 Buffered Listeria Enrichment Fraser Broth, Modified Broth Selective Supplement Fraser Broth, Modified is recommended For use as a supplement for Buffered for the selective enrichment of Listeria Listeria Enrichment Broth Base (BAM spp. in foods and environmental samples. Formulation), Cat. no. U233. 16x125mm tube, 20/pk K98 15x103mm tube, 20/pk K183 500ml PC bottle, 10/pk U410 Oxford Medium For the selective isolation and CompactDry™ LS differentiation of Listeria monocytogenes. Listeria 15x100mm plate, 10/pk G46 For the selective growth, presumptive identification, and enumeration of Listeria spp. Growing colonies will appear blue. Motility Test Medium 100/pk LS100 Recommended for the detection of motility in gram-negative enteric bacilli. 16x100mm tube, 20/pk Q10 3 Salmonella spp. Media HE (Hektoen Enteric) Agar XLD (Xylose Lysine Deoxycholate) Hektoen Enteric Agar (HE) is for the Agar, USP selective isolation and differentiation of XLD Agar, USP is recommended for use pathogenic gram-negative enteric bacteria, as a selective and differential medium such as the non-lactose fermenting for the isolation of gram-negative enteric Salmonella spp. and Shigella spp. pathogens. 15x100mm plate, 10/pk G63 15x100mm plate, 10/pk G65 Tetrathionate Broth Base Rappaport-Vassiliadis Broth, For selective enrichment of Salmonella spp. Use with iodine-iodide solution, USP Cat. no. Z129. Recommended for the selective enrichment of Salmonella spp. from food samples. 16x125mm tube, 20/pk K65 16x125mm tube, 20/pk K246 Iodine-Iodide Solution, for HardyCHROM™ Salmonella Tetrathionate Broth HardyCHROM™ Salmonella is a differential Iodine-Iodide Solution to be added to medium that facilitates the isolation Tetrathionate Broth, Cat. No. K65. and differentiation of Salmonella spp. 2 oz glass blottle, EA Z129 from other members of the family Enterobacterales. Selenite Cystine Broth 15x100mm plate, 10/pk For the selective enrichment of Salmonella spp. CompactDry™ SL 15ml dropper vial, 20/pk K69 Salmonella For the presumptive identification of Salmonella spp. Growing colonies will appear green or blue-green with black centers. 240/pk 54085 Lactose Broth For the cultivation of coliforms and Salmonella spp. in water, food, and dairy. 12 oz. wide mouth jar, 12/pk U146 Lysine Iron Agar (LIA) For the identification of gram-negative enteric pathogens. 16x125mm tube, 20/pk L25 MR-VP Broth For the identification of gram-negative bacilli. 15x103mm tube, 20/pk K37 Cat. no. G309 4 General Culture Media MacConkey Agar, USP m Endo LES Agar For the isolation and differentiation of For the enumeration of coliforms from gram-negative bacteria. water specimens by membrane filter 15x100mm plate, 10/pk G35 technique. 15x100mm plate, 10/pk G28 Brilliant Green Bile Broth For the detection of coliforms in water Violet Red Bile Agar (VRBA) and food. Contains a durham tube for gas with MUG detection. For the detection of coliforms in food or 16x125mm tube, 20/pk K66 dairy products. 15x100mm plate, 10/pk J132 Lauryl Tryptose Broth (Lauryl Sulfate Broth) SabDex (Sabouraud Dextrose) For the detection of coliforms in water and Agar, USP food. Double strength. Contains a durham Recommended for the isolation, tube for gas detection. cultivation, and maintenance of non- 20x125mm tube, 20/pk K32 pathogenic and pathogenic species of fungi and yeasts. Koser Citrate Broth 15x100mm, 10/pk W70 Used primarily for coliform determinations from water and waste water samples. BHI Broth 13x100mm tube, 20/pk R135 For the cultivation of microorganisms. 16x125mm tube, 20/pk K25 EMB Levine Agar (Eosin Methylene Blue) For isolation and differentiation of gram-negative bacilli. 15x100mm, 10/pk G25 5 General Culture Media Egg Yolk Agar, Modified Tryptic Soy Agar (TSA), USP For the isolation, cultivation, and For use as a non-selective, general growth differentiation of Clostridium spp. and medium for the isolation and cultivation of other anaerobic bacteria. microorganisms (bacteria, yeast, 15x100mm, 10/pk G215 and fungi). 15x100mm, 10/pk G60 Reinforced Clostridial Medium, 20x125mm tube, 20/pk Q58 USP For the cultivation and enumeration of Blood Agar Plate, 5% Sheep Clostridia spp. and other anaerobic and Blood in Tryptic Soy Agar facultative bacteria. (TSA) Base 4 oz Glass Bottle, 20/pk U172 Recommended for use as general purpose growth media for the isolation, cultivation, and differentiation of a wide variety of Standard Methods Agar microorganisms. For the cultivation and enumeration of microorganisms in dairy products, food, 15x100mm, 10/pk A10 and water. 15x100mm, 10/pk G43 20x125mm tube, 20/pk Q21 6 Control Organisms STEC/EHEC Big 6 Organisms (listed in KWIK-STIK™ format) 2 Pack Escherichia coli, serotype O103:H11, CDC06-3008 (STEC) 01101P Escherichia coli, serotype O111:H8, CDC2010C-3114 (STEC) 01102P Escherichia coli, serotype O121:H19, CDC02-3211 (STEC) 01099P Escherichia coli, serotype O145:NM, CDC99-3311 (STEC) 01097P Escherichia coli, serotype O26:H11, CDC 03-3014 (STEC) 01100P Escherichia coli, serotype O45:H2, CDC00-3039 (STEC) 01098P E. coli O157 Organisms (listed in KWIK-STIK™ format) 2 Pack Escherichia coli (O157:H7), ATCC® 35150™ 0617P E. coli non-STEC/EHEC Organisms (listed in KWIK-STIK™ format) 2 Pack Escherichia coli, ATCC® 25922™ 0335P Yeast and Mold Organisms (listed in KWIK-STIK™ format) 2 Pack Candida albicans, ATCC® 10231 0443P Aspergillus brasiliensis, ATCC® 16404™ 0392P Listeria monocytogenes Organisms (listed in KWIK-STIK™ format) 2 Pack Listeria monocytogenes, ATCC® 15313™ 0727P Salmonella enterica Organisms (listed in KWIK-STIK™ format) 2 Pack Salmonella enterica, ATCC® 14028™ 0363P 7 Dry Media Plates The CompactDry™ plate line presents a variety of organism specific or total count tests, packaged nicely in a sophisticated and sturdy cassette. • Sturdy frame, stackable • Stored at room temperature packaging • No leakage • Self-diffusing media • Easy to label • Ability to subculture without damage to colonies CompactDry™ TC Total Count For total aerobic viable bacterial count. Growing colonies will appear red. 240/pk 54081 CompactDry™ EC E. coli and Coliforms For the isolation and enumeration of coliforms and E. coli. Coliform colonies will appear red, and E. coli colonies will appear blue. 240/pk 54082 CompactDry™ YM Yeast and Mold For the isolation and enumeration of yeast and mold. Yeast colonies will appear blue, and molds will form cottony colonies with characteristic colors. (5-7 days) 240/pk 54083 CompactDry™ YMR Yeast and Mold Rapid For the isolation and enumeration of yeast and mold. Yeast colonies will appear blue, and molds will form cottony colonies with characteristic colors. (48-72 hrs) 240/pk 54084 8 Dry Media Plates CompactDry™ XSA Staphylococcus aureus For the selective growth, enumeration, and differentiation of Staphylococcus aureus. Growing colonies will appear blue green. 240/pk 54086 CompactDry™ ETB Enterobacterales
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