Revision: 2 Effective Date: 2/24/2020 hazy. Appearance: Prepared Appearance: Dehydrated Specifications Control Quality organisms. enteric of identification and the isolation for Agar MacConkey using references toappropriate Refer Procedure Test 4. 3. 2. 1. Preparation toSDS Refer Precaution specifications performance to meet required as supplemented and/or adjusted be may Formula 25ºC at 0.2 ± 7.1 Final pH: Agar Violet Crystal Red Neutral Chloride Sodium Salts Bile Lactose Tissue Animal of Digest Enzymatic Casein of Digest Enzymatic Gelatin of Digest Enzymatic Typical Formulation Agar. MacConkey USP/EP/JP as such Non bacteria. lactose fermenting around or halo precipitate a bile in resulting bile s of precipitation the will cause production acid Enough colonies. pink in resulting red neutral coli Escherichia seleas violet act crystal and Bile salts balance. osmotic the maintains chloride sodium and carbohydrate is a fermentable Lactose nitrogen. and carbon non from Harmonized bythe recommended A medium Description in humans. conditions or other disease of diagnosis in the use for intended is not setting laboratory Agar MacConkey Use Intended Sheet Specification Technical
Cool to 45 toCool minutes. 15 for 121ºC at Autoclave medium. the dissolve completely to minute one boil for and agitation frequent with Heat water. purified inof one liter the medium of 50 g Suspend
Salmonella spp. spp. Salmonella - sterile products. Conforms to USP/EP/JP performance specification. Gelatin serves as source of of source as serves specification. Gelatin performance to USP/EP/JP Conforms sterile products. Mixture
, MacConkey Broth is used as a as is used Broth MacConkey ,
-
50°C.
can ferment lactose to produce acid which results in a pH drop. This is indicated by by indicated is This pH drop. a in which results acid produce to lactose ferment can
.
Gram of differentiation and the isolation for is used Conforms Conforms
Prepared MacConkey Agar is medium to dark pink to dark is medium Agar MacConkey Prepared grow but remain colorless with no bile precipitate. According to the Harmonized Harmonized the to According precipitate. withbile no colorless remain but grow
Powder is homogeneous, free flowing, and light pink light and flowing, free homogeneous, is Powder
to Harmonized to Harmonized MacConkeyAgar(NCM0017)
0.001 g/L 0.001
13.5 g/L 13.5 g/L0.03 10 17 5 g/L 1.5 g/L 1.5 g/L 1.5 .0 .0 .0
selective enrichment broth, with subculture performed onto onto performed subculture with broth, enrichment selective USP/EP/JP Requirements and FDA/BAM. Requirements USP/EP/JP
USP/EP/JP g/L g/L g/L ctive agents inhibiting many Gram many inhibiting agents ctive
for isolation and identification of of identification and isolation for
- negative enteric bacilli negative enteric
- purple and trace to slightly slightly to trace and purple - lactose fermenting bacteria bacteria fermenting lactose -
beige.
- positive bacteria. bacteria. positive
MacConkey Agar Agar MacConkey Escherichia coli Escherichia
in a in a .
alts alts
Revision: 2 Effective Date: 2/24/2020 9. 8. 7. 6. 5. 4. 3. 2. 1. References closed. tightly container by keeping and light moisture from Protect storage temperature. the same at environment humidity low in a container d Store Storage 3. 2. 1 Procedure the of Limitations directed. as when stored container in its intact medium to applies Expiry the originalcolor. from changed has appearance if or flowing, The on container. stamped date toexpiration Refer Expiration colonies. orclear grow colorless as organisms lactose fermenting Lactose Results qualitycontrol used thatfor beshould minimum the listed are organisms The times. incubation and temperatures specified Response: Cultural Expected Sheet Specification Technical
typhimurium Salmonella Staphylococcus aureus Staphylococcus Enterococcus faecalis Enterococcus MICROORGANISM examination of food, food, of examination (eds.). Splittstoesser D. F. and C., Vanderzant, t.htm. www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalyticalmanualBAM/defaul D.C. Association,Washington, Public Health (ed.). T. R. Marshall, D.C. Washington, Microbiology, for Society American ed. 6th microbiology, R.H. and C. Tenover, F. Pfaller, M. A. Baron, E. J. R., P. Murray, 2011 March 5,Rev PI7102, A. MacConkey, 17 Pharmacopeia Japanese Pharmacopeia States United Pharmacopoeia European strains Gram of of a number of recovery CO increased under plates Agar MacConkey of Incubation comp for arerecommended cultures pure using testing and serological biochemical Gram for primarily medium is a selective Agar MacConkey Although medium. this on grow fail to or poorly grow that encountered strains maybe Some Proteus mirabilis Proteus Escherichia coli Escherichia coli Escherichia ehydrated culture media culture ehydrated - fermenting organisms grow as pink colonies with or without a zone of precipitated bile. Non bile. precipitated zone of without a or with colonies pink grow as organisms fermenting
1905. Lactose 1905.
4th Standard methods for the examination of dairy products, 1 products, dairy of the examination for methods Standard
ed. American Public Health Ass Health Public American ed. 14028 29906 25922 29212 ATCC 6538 8739 10
Cultural response on MacConkey Agar tested at Harmonized USP/EP/JP USP/EP/JP Harmonized at tested Agar MacConkey on response Cultural at th
th National Formulary 201 Formulary National Edition (2017) Edition -
2
fermenting bacteria in feces. J. Hyg. Hyg. J. feces. in bacteria fermenting
Edition (20 Edition
- 30 INOC °C APPROX. APPROX. 10 10 10 10 >1000 >1000 (CFU)
away from direct sunlight. direct sunlight. from away - - - - - ULUM ULUM 100 100 100 100 negative bacilli.
20
)
2015 dehydrated medium should be discarded if not free not free if discarded be should medium dehydrated Recovery 70 70 70 70 Inhibited Inhibited . Compendium of methods for the microbiological for the microbiological of methods Compendium . - - - - 8 200% 200% 200% 200% : USP 4 USP : ociation, Washington, D.C. D.C. ociation, Washington, 2
has been reported to reduce growth and and growth reduce to reported been has
1 Colorless colonies; partial inhibition of swarming of inhibition partial colonies; Colorless
NF 3 NF Once opened and recapped, place place and recapped, opened Once Yolken (eds.). (eds.). Yolken 5 - EXPECTED RESULTS EXPECTED negative enteric bacilli, bacilli, enteric negative 6 :333
testing. - 379. 379. Pink colonies w/ bile ppt. w/ bile colonies Pink ppt. w/ bile colonies Pink
Colorless colonies Colorless
7
Manual of clinical of Manual th ed. American American ed. th Reaction
lete identification. identification. lete
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-
Revision: 2 Effective Date: 2/24/2020 12. 11. 10. Sheet Specification Technical
incubation. Abst. Ann. Mtg. American Society for Microbiolog Society for American Mtg. Ann. Abst. incubation. Mazura 20 Che OfficialAnalytical of Association 19t wastewater, water and of (eds.). E. Greenberg A. and S. Clesceri, L.A. D., Eaton, th ed. AOAC International. Arlington, VA. VA. Arlington, International. AOAC ed. th - Reetz, G. T. Neblett, and J. M.Galperin. M.Galperin. J. and Neblett, T. G. Reetz, h ed. American Public Health Association, Washington, D.C. D.C. Association, Washington, Public Health American ed. mists. mists. 2016
. Official methods of analysis of AOAC International, AOAC International, of analysis of methods . Official 1979. MacConkey Agar: CO2 vs. ambient ambient vs. CO2 Agar: MacConkey 1979. 2017 y. C179.
. Standard methods for the examination the examination for methods . Standard