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( 12 ) United States Patent ( 10 ) Patent No .: US 10,813,359 B2 Sword ( 45 ) Date of Patent : Oct
US010813359B2 ( 12 ) United States Patent ( 10 ) Patent No .: US 10,813,359 B2 Sword ( 45 ) Date of Patent : Oct. 27 ,2 2020 ( 54 ) FUNGAL ENDOPHYTES FOR IMPROVED 6,689,880 B2 2/2004 Chen et al . CROP YIELDS AND PROTECTION FROM 6,823,623 B2 11/2004 Minato et al . 7,037,879 B2 5/2006 Imada et al . PESTS 7,080,034 B1 7/2006 Reams 7,084,331 B2 8/2006 Isawa et al . ( 71 ) Applicant: THE TEXAS A & M UNIVERSITY 7,335,816 B2 2/2008 Kraus et al . SYSTEM , College Station , TX (US ) 7,341,868 B2 3/2008 Chopade et al . 7,485,451 B2 2/2009 VanderGheynst et al . 7,555,990 B2 7/2009 Beaujot ( 72 ) Inventor: Gregory A. Sword , College Station , 7,632,985 B2 12/2009 Malven et al . TX (US ) 7,763,420 B2 7/2010 Stritzker et al . 7,906,313 B2 3/2011 Henson et al . ( 73 ) Assignee : THE TEXAS A & M UNIVERSITY 7,977,550 B2 7/2011 West et al . SYSTEM , College Station , TX (US ) 8,019,694 B2 9/2011 Fell et al . 8,143,045 B2 3/2012 Miansnikov et al . 8,455,198 B2 6/2013 Gao et al . ( * ) Notice: Subject to any disclaimer , the term of this 8,455,395 B2 6/2013 Miller et al . patent is extended or adjusted under 35 8,465,963 B2 6/2013 Rolston et al . U.S.C. 154 ( b ) by 0 days. 8,728,459 B2 5/2014 Isawa et al. 9,113,636 B2 1/2015 von Maltzahn et al . -
Characterization of Alternaria Alternata Isolates Causing Brown Spot of Potatoes in South Africa
Characterization of Alternaria alternata isolates causing brown spot of potatoes in South Africa By Joel Prince Dube Submitted in partial fulfilment of the requirements for the degree of Master in Science (Agriculture) Plant Pathology In the faculty of Natural and Agricultural Sciences Department of Microbiology and Plant Pathology University of Pretoria Pretoria February 2014 © University of Pretoria DECLARATION I, Joel Prince Dube, declare that the thesis, which I hereby submit for the degree Master of Science (Agriculture) Plant Pathology at the University of Pretoria, is my own work and has not been previously submitted by me for a degree at this or any other tertiary institution. Signed: ___________________________ Date: ____________________________ i © University of Pretoria Acknowledgements I would like to extend my heartfelt thanks the contributions of the following: 1. First and foremost, the Almighty God by whose grace I am where I am today. I owe everything to him. 2. My supervisors, Prof. Jacquie van der Waals and Dr. Mariette Truter, for their unwavering support and guidance throughout my Masters journey. 3. Pathology programme @ UP for the opportunity and funding for my studies. 4. Syngenta for funding one of my chapters. 5. Charles Wairuri, Nelisiwe Khumalo, Alain Misse for their help with all my molecular work. 6. Colleagues in greenhouse for all their help, suggestions and contributions throughout my studies. 7. My family and friends for their financial, spiritual and moral support, it is greatly appreciated. ii © University of Pretoria Characterization of Alternaria alternata isolates causing brown spot of potatoes in South Africa By Joel Prince Dube Supervisor : Prof. J. -
The Incidence of Alternaria Species Associated with Infected Sesamum Indicum L
Plant Pathol. J. 1-11 (2017) https://doi.org/10.5423/PPJ.OA.04.2017.0081 The Plant Pathology Journal pISSN 1598-2254 eISSN 2093-9280 ©The Korean Society of Plant Pathology Research Article Open Access The Incidence of Alternaria Species Associated with Infected Sesamum indicum L. Seeds from Fields of the Punjab, Pakistan Brian Gagosh Nayyar1*, Steve Woodward2, Luis A. J. Mur3, Abida Akram1, Muhammad Arshad1, S. M. Saqlan Naqvi4, and Shaista Akhund1 1Department of Botany, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan 2Institute of Biological and Environmental Sciences, University of Aberdeen, Cruikshank Building, St. Machar Drive, Aberdeen AB24 3UU, Scotland, UK 3Institute of Biological, Rural and Environmental Sciences, Aberystwyth University, Edward Llwyd Building, Penglais Campus, Aberystwyth SY23 3DA, Wales, UK 4Department of Biochemistry, Pir Mehr Ali Shah Arid Agriculture University, Rawalpindi 46300, Pakistan (Received on April 10, 2017; Revised on July 9, 2017; Accepted on July 23, 2017) Sesame (Sesamum indicum) is an important oil seed (KP123850.1) in GenBank accessions. The pathogenic- crop of Asia. Yields can be negatively impacted by vari- ity and virulence of these isolates of Alternaria alternata ous factors, including disease, particularly those caused was confirmed in inoculations of sesame plants result- by fungi which create problems in both production and ing in typical symptoms of leaf blight disease. This storage. Foliar diseases of sesame such as Alternaria leaf work confirms the identity of a major source of sesame blight may cause significant yield losses, with reductions leaf blight in Pakistan which will aid in formulating ef- in plant health and seed quality. -
Major Diseases of Horticultural Crops and Their Management
Major Diseases of Horticultural Crops and this Management Dr.G. Thiribhuvanamala, Dr.S.Nakkeeran and Dr. K.Eraivan Arutkani Aiyanathan Department of Plant Pathology Centre for Plant Protection Studies Tamil Nadu Agricultural University Coimbatore – 641 003 Introduction: The Horticulture (fruits including nuts, vegetables including potato, tuber crops, mushroom, ornamental plants including cut flowers, spices, plantation crops and medicinal and aromatic plants) has become a key drivers for economic development in many of the states in the country and it contributes 30.4 per cent to GDP of agriculture, which calls for knowledge and technical backstopping. Intensive cultivation of the high valued horticultural crops, resulted in the outbreak of several diseases of National importance. In recent days, stakeholders import planting materials from North American Countries. Introduction of planting materials also impose threat in the introduction of new diseases not known to be present earlier. However, the diseases, if not managed on a war foot, it will result in drrastic yield reduction and quality of the produces. Hence adoption of suitable management measures with low residue levels in the final produces becomes as a need of the hour. In this regard, this paper gives emphasis on the diagnosis of plant diseases and their management. 293 Diseases of Mango and their management 1. Anthracnose :Colletotrichum gloeosporioides Anthracnose symptoms occur on leaves, twigs, petioles, flower clusters (panicles), and fruits. The incidence of this disease can reach almost 100% in fruit produced under wet or very humid conditions. On leaves, lesions start as small, angular, brown to black spots and later enlarge to form extensive dead areas. -
Compendium File
ICAR SPONSORED SHORT COURSE ON “MAKING GRETAER USE OF BIOCONTROL AGENTS FOR ORGANIC AGRICULTURE”, 4-13 NOV, 2019; AAU: JORHAT:ASSAM 1 ICAR Sponsored Short Course On “Making Greater Use of Biocontrol Agents For Organic Agriculture” Organized by: Department of Plant Pathology Assam Agricultural University Jorhat- 785013, Assam ICAR SPONSORED SHORT COURSE ON “MAKING GRETAER USE OF BIOCONTROL AGENTS FOR ORGANIC AGRICULTURE”, 4-13 NOV, 2019; AAU: JORHAT:ASSAM 2 The publication has been brought out on the occasion of a Short Course on “Making Greater Use of Biocontrol Agents for Organic Agriculture” sponsored by ICAR, New Delhi and organized by Department of Plant Pathology, Assam Agricultural University, Jorhat- 13, Assam from 4th to 13th November, 2019 Editorial Board Dr. Pranab Dutta Dr. B. C. Das Dr. D. K. Sarmah Member Ms. Arti Kumari Cover design: Mr. Gaurav Phookan ©Department of Plant Pathology, Assam Agricultural University, Jorhat-13, Assam ICAR SPONSORED SHORT COURSE ON “MAKING GRETAER USE OF BIOCONTROL AGENTS FOR ORGANIC AGRICULTURE”, 4-13 NOV, 2019; AAU: JORHAT:ASSAM 3 CONTENT Chapter Title No. 1. An overview of AAU’s achievement on biological control 2. Trichoderma- the most powerful arsenal for plant disease management 3. Exploitation of biological agents for management of viral diseases of agricultural crops 4. Biological Control- an ecological perspective 5. Mass production technology of Beauveria bassiana 6. Morphological characterization of fungal bio-control agents 7. Mass production technology of Trichoderma harzianumwith locally available substrates 8. Mass culture technique of Biocontrol agents for management of mites of agricultural crops 9. Agro-forestry and Organic Farming 10. -
Communications
COMMUNICATION S FACULTY OF SCIENCES DE LA FACULTE DES SCIENCES UNIVERSITY OF ANKARA DE L’UNIVERSITE D’ANKARA Series C: Biology VOLUME: 29 Number: 1 YEAR: 2020 Faculy of Sciences, Ankara University 06100 Beşevler, Ankara-Turkey ISSN: 1303-6025 E-ISSN: 2651-3749 COMMUNICATION S FACULTY OF SCIENCES DE LA FACULTE DES SCIENCES UNIVERSITY OF ANKARA DE L’UNIVERSITE D’ANKARA Series C: Biolog y Volume 29 Number : 1 Year: 2020 Owner (Sahibi) Selim Osman SELAM, Dean of Faculty of Sciences Editor-in-Chief (Yazı İşleri Müdürü) Nuri OZALP Managing Editor Nur Münevver PINAR Area Editors Ilgaz AKATA (Botany) Nursel AŞAN BAYDEMİR (Zoology) İlker BUYUK (Biotechnology) Talip ÇETER (Plant Anatomy and Embryology) Ilknur DAĞ (Microbiology, Histology) Türker DUMAN (Moleculer Biology) Borga ERGONUL (Hydrobiology) Sevgi ERTUĞRUL KARATAY (Biotechnology) Esra KOÇ (Plant Physiology) G. Nilhan TUĞ (Ecology) A. Emre YAPRAK ( Botany) Mehmet Kürşat Şahin (Zoology) Şeyda Fikirdeşici Ergen (Hydrobiology) Alexey YANCHUKOV (Populations Genetics, Molecular Ecology and Evolution Biology) Language Editor: Sümer ARAS Technical Editor: Aydan ACAR ŞAHIN Editors Nuray AKBULUT (Hacettepe University, Turkey) Hasan AKGUL (Akdeniz University, Turkey) Şenol ALAN (Bülent Ecevit University, Turkey) Dirk Carl ALBACH (Carl Von Ossietzky University, Germany) Ahmet ALTINDAG (Ankara University, Turkey) Rami ARAFEH (Palestine Polytechnic University, Palestine) Belma BINLI ASLIM (Gazi University, Turkey) Tahir ATICI (Gazi University,Turkey) Dinçer AYAZ (Ege University, Turkey) Zeki AYTAÇ (Gazi University,Turkey) Jan BREINE (Research Institute for Nature and Forest, Belgium) Kemal BUYUKGUZEL (Bulent Ecevit University, Turkey) Suna CEBESOY (Ankara University, Turkey) A. Kadri ÇETIN (Fırat University, Turkey) Nuran ÇIÇEK (Hacettepe University, Turkey) Elif SARIKAYA DEMIRKAN (Uludag University, Turkey) Mohammed H. -
Studies in Mycology 75: 171–212
STUDIES IN MYCOLOGY 75: 171–212. Alternaria redefined J.H.C. Woudenberg1,2*, J.Z. Groenewald1, M. Binder1 and P.W. Crous1,2,3 1CBS-KNAW Fungal Biodiversity Centre, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands; 2Wageningen University and Research Centre (WUR), Laboratory of Phytopathology, Droevendaalsesteeg 1, 6708 PB Wageningen, The Netherlands; 3Utrecht University, Department of Biology, Microbiology, Padualaan 8, 3584 CH Utrecht, The Netherlands *Correspondence: Joyce H.C. Woudenberg, [email protected] Abstract: Alternaria is a ubiquitous fungal genus that includes saprobic, endophytic and pathogenic species associated with a wide variety of substrates. In recent years, DNA- based studies revealed multiple non-monophyletic genera within the Alternaria complex, and Alternaria species clades that do not always correlate to species-groups based on morphological characteristics. The Alternaria complex currently comprises nine genera and eight Alternaria sections. The aim of this study was to delineate phylogenetic lineages within Alternaria and allied genera based on nucleotide sequence data of parts of the 18S nrDNA, 28S nrDNA, ITS, GAPDH, RPB2 and TEF1-alpha gene regions. Our data reveal a Pleospora/Stemphylium clade sister to Embellisia annulata, and a well-supported Alternaria clade. The Alternaria clade contains 24 internal clades and six monotypic lineages, the assemblage of which we recognise as Alternaria. This puts the genera Allewia, Brachycladium, Chalastospora, Chmelia, Crivellia, Embellisia, Lewia, Nimbya, Sinomyces, Teretispora, Ulocladium, Undifilum and Ybotromyces in synonymy with Alternaria. In this study, we treat the 24 internal clades in the Alternaria complex as sections, which is a continuation of a recent proposal for the taxonomic treatment of lineages in Alternaria. -
A Worldwide List of Endophytic Fungi with Notes on Ecology and Diversity
Mycosphere 10(1): 798–1079 (2019) www.mycosphere.org ISSN 2077 7019 Article Doi 10.5943/mycosphere/10/1/19 A worldwide list of endophytic fungi with notes on ecology and diversity Rashmi M, Kushveer JS and Sarma VV* Fungal Biotechnology Lab, Department of Biotechnology, School of Life Sciences, Pondicherry University, Kalapet, Pondicherry 605014, Puducherry, India Rashmi M, Kushveer JS, Sarma VV 2019 – A worldwide list of endophytic fungi with notes on ecology and diversity. Mycosphere 10(1), 798–1079, Doi 10.5943/mycosphere/10/1/19 Abstract Endophytic fungi are symptomless internal inhabits of plant tissues. They are implicated in the production of antibiotic and other compounds of therapeutic importance. Ecologically they provide several benefits to plants, including protection from plant pathogens. There have been numerous studies on the biodiversity and ecology of endophytic fungi. Some taxa dominate and occur frequently when compared to others due to adaptations or capabilities to produce different primary and secondary metabolites. It is therefore of interest to examine different fungal species and major taxonomic groups to which these fungi belong for bioactive compound production. In the present paper a list of endophytes based on the available literature is reported. More than 800 genera have been reported worldwide. Dominant genera are Alternaria, Aspergillus, Colletotrichum, Fusarium, Penicillium, and Phoma. Most endophyte studies have been on angiosperms followed by gymnosperms. Among the different substrates, leaf endophytes have been studied and analyzed in more detail when compared to other parts. Most investigations are from Asian countries such as China, India, European countries such as Germany, Spain and the UK in addition to major contributions from Brazil and the USA. -
1. Padil Species Factsheet Scientific Name: Common Name Image
1. PaDIL Species Factsheet Scientific Name: Alternaria dauci (J.G. Kühn) J.W. Groves & Skolko (Ascomycota: Dothideomycetes: Pleosporales: Pleosporaceae) Common Name Alternaria dauci Live link: http://www.padil.gov.au/maf-border/Pest/Main/142987 Image Library New Zealand Biosecurity Live link: http://www.padil.gov.au/maf-border/ Partners for New Zealand Biosecurity image library Landcare Research — Manaaki Whenua http://www.landcareresearch.co.nz/ MPI (Ministry for Primary Industries) http://www.biosecurity.govt.nz/ 2. Species Information 2.1. Details Specimen Contact: Eric McKenzie - [email protected] Author: McKenzie, E. Citation: McKenzie, E. (2013) Alternaria dauci(Alternaria dauci)Updated on 5/7/2014 Available online: PaDIL - http://www.padil.gov.au Image Use: Free for use under the Creative Commons Attribution-NonCommercial 4.0 International (CC BY- NC 4.0) 2.2. URL Live link: http://www.padil.gov.au/maf-border/Pest/Main/142987 2.3. Facets Commodity Overview: Field Crops and Pastures Commodity Type: Apiaceous produce Distribution: Oceania, Afrotropic, Antarctic, Australasia, Indo-Malaya, Nearctic, Neotropic, Palearctic Groups: Fungi & Mushrooms Host Family: Apiaceae Pest Status: 1 NZ - Non-regulated species Status: NZ - Exotic 2.4. Diagnostic Notes **Disease** Leaf blight. Irregular, dark brown blotches on leaves and petioles. All above ground parts of the plant may be killed progressively from the outer leaves inwards, with a reduction in root development. **Morphology** _Conidiophores_ arising singly or in small groups, -
Statement of Purpose
Diseases of Specific Florist Crops Keeping florist crops free of disease requires constant care and planning. Prevention is the basis of freedom from disease and should be an integral part of the general cultural program. The symptoms of the diseases of major florist crops are described individually by crop in a series of fact sheets. Carnation (Dianthus caryophyllus) Note: Use culture-indexed plants, and steam-pasteurize or fumigate growing media and beds. • Fusarium wilt (Fusarium oxysporum f. sp. dianthi): Stunting, wilting, foliar yellowing or browning, purplish vascular discoloration. • Rhizoctonia stem rot: Brown canker at the soil line. • Alternaria leaf spot (Alternaria dianthi): Ashy white spots with dark fungus spore structures on the center of older spots. • Bacterial wilt (Pseudomonas caryophylli): Sudden wilting and drying of the top of the plant or of one branch. Vascular system shows yellow streaks. Root system also decays, and sticky cankered areas may appear at the base of the stem. Symptoms appear at high temperatures. Discard infected plants immediately. Fortunately, this disease is eliminated by culture indexing. • Fusarium stem rot (Fusarium roseum): Roots and stem base may be rotted and discolored. • Botrytis flower spot: First shows on outer petals on outside of bud. • Rust (Uromyces caryophyllinus): Reddish-brown spore pustules on leaves and stems. • Fusarium bud rot (Fusarium tricinctum poae): Fungus spores are carried to the buds by grass mites. Petals inside bud decay first. • Greasy blotch (Zygophiala jamaicensis): Spots with radiating, spider web-like margins. . -
Fungal Flora of Korea
Fungal Flora of Korea Volume 1, Number 2 Ascomycota: Dothideomycetes: Pleosporales: Pleosporaceae Alternaria and Allied Genera 2015 National Institute of Biological Resources Ministry of Environment Fungal Flora of Korea Volume 1, Number 2 Ascomycota: Dothideomycetes: Pleosporales: Pleosporaceae Alternaria and Allied Genera Seung Hun Yu Chungnam National University Fungal Flora of Korea Volume 1, Number 2 Ascomycota: Dothideomycetes: Pleosporales: Pleosporaceae Alternaria and Allied Genera Copyright ⓒ 2015 by the National Institute of Biological Resources Published by the National Institute of Biological Resources Environmental Research Complex, Hwangyeong-ro 42, Seo-gu Incheon, 404-708, Republic of Korea www.nibr.go.kr All rights reserved. No part of this book may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, electronic, mechanical, photocopying, recording, or otherwise, without the prior permission of the National Institute of Biological Resources. ISBN : 9788968111259-96470 Government Publications Registration Number 11-1480592-000905-01 Printed by Junghaengsa, Inc. in Korea on acid-free paper Publisher : Kim, Sang-Bae Author : Seung Hun Yu Project Staff : Youn-Bong Ku, Ga Youn Cho, Eun-Young Lee Published on March 1, 2015 The Flora and Fauna of Korea logo was designed to represent six major target groups of the project including vertebrates, invertebrates, insects, algae, fungi, and bacteria. The book cover and the logo were designed by Jee-Yeon Koo. Preface The biological resources represent all the composition of organisms and genetic resources which possess the practical and potential values essential for human lives, and occupies a firm position in producing highly value-added products such as new breeds, new materials and new drugs as a means of boosting the national competitiveness. -
Changes in the Core Endophytic Mycobiome of Carrot Taproots In
www.nature.com/scientificreports OPEN Changes in the core endophytic mycobiome of carrot taproots in response to crop management and genotype Sahar Abdelrazek1, Sulbha Choudhari2,3, Jyothi Thimmapuram3, Philipp Simon4, Micaela Colley5, Tesfaye Mengiste6 & Lori Hoagland1* Fungal endophytes can infuence production and post-harvest challenges in carrot, though the identity of these microbes as well as factors afecting their composition have not yet been determined, which prevents growers from managing these organisms to improve crop performance. Consequently, we characterized the endophytic mycobiome in the taproots of three carrot genotypes that vary in resistance to two pathogens grown in a trial comparing organic and conventional crop management using Illumina sequencing of the internal transcribed spacer (ITS) gene. A total of 1,480 individual operational taxonomic units (OTUs) were identifed. Most were consistent across samples, indicating that they are part of a core mycobiome, though crop management infuenced richness and diversity, likely in response to diferences in soil properties. There were also diferences in individual OTUs among genotypes and the nematode resistant genotype was most responsive to management system indicating that it has greater control over its endophytic mycobiome, which could potentially play a role in resistance. Members of the Ascomycota were most dominant, though the exact function of most taxa remains unclear. Future studies aimed at overcoming difculties associated with isolating fungal endophytes are needed to identify these microbes at the species level and elucidate their specifc functional roles. Carrot (Daucus carota L. subsp. sativus (Hofm.) Arcang.) is one of the most important vegetable crops in the world, providing a good source of beta-carotene, fber, Vitamin A and other vitamins and minerals to the human diet1,2.