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Appendix a Standard Operating Procedures

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Appendix a Standard Operating Procedures Appendix A Standard Operating Procedures Date: August 2008 List of Standard Operating Procedures (SOP’s) Revision: 4 Author: Marie Esten University of Rhode Island Watershed Watch Description SOP Number General Laboratory Safety 001 University Safety and Waste Handling Document 001a Laboratory Water 002 General Labware Cleaning Procedure 003 General Autoclave Operation 004 Bottle Autoclaving Procedure 005 Waste Autoclaving Procedure 006 Ambient Waters Microbiological Procedure 007 ISDS Microbiological Procedure 008 Total Suspended Solids Analysis 009 Alkalinity and pH Procedures 010 Biochemical Oxygen Demand Procedure 011 Chlorophyll-A Analysis, Welschmeyer Method 012 Chloride Analysis 013 Ammonia Analysis 014 Orthophosphate and Nitrate + Nitrite Analysis 015 Total Phosphorus and Nitrogen Analysis 016 Salinity Analysis 017 Enterococci Analysis Using Enterolert IDEXX Method 018 Analytical Balance Calibration 019 Heterotrophic Plate Count 020 Standard Operating Procedure List S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP-List.doc Standard Operating Procedure 001 Date: 11/04 General Laboratory Safety Revision: 1 Author: Linda Green University of Rhode Island Watershed Watch 1.0 PURPOSE AND DESCRIPTION LAB SAFETY IS EVERYBODY’S JOB! Please be sure to familiarize yourself with these general procedures, as well as the specific handling requirements included in the Standard Operating Procedure (SOP) for each analysis/process. Further general information regarding University of Rhode Island standards for health and safety are found in SOP 001a – University Safety and Waste Handling Document. 2.0 HEALTH AND SAFETY 2.1 Emergency Numbers: EMERGENCIES: 874-2121 UNCONTAINED SPILLS: 874- 2618 GENERAL HEALTH AND SAFETY INFORMATION URI SAFETY AND RISK: 874-2618 2.2 General Health and Safety Information 1. Eye protection, gloves and lab coats are REQUIRED in this laboratory when working with chemicals. 2. Closed-toed shoes are REQUIRED whenever you are working in the lab. Even if you are just entering data. 3. Know where the accident and safety equipment is: First–aid kit: i. Behind the sink in room 018. ii. On top of the refrigerator in room 002. iii. On top of the refrigerator in room 019. Safety shower: next to the entry door of each laboratory. Eye wash: at main sink in each laboratory. Yellow Spill pads: i. Under the laboratory bench to the right of blue oven in room 018. ii. Next to the main sink in room 002. iii. On top of the refrigerator in room 019 General Laboratory Safety SOP 001 1 of 2 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 001 Safety-mod.doc 4. Report ANY accidents IMMEDIATELY to Linda Green (874-2905) or Elizabeth Herron (874- 4552). If neither are available, first contact staff in the Cooperative Extension Water Quality Suite (room 001), Dr. Art Gold (874-2903) or department secretary (874-2026). Report all emergencies to 874-2121. 5. If you spill anything hazardous on yourself immediately flush it with water for 15 minutes. Report all emergencies to 874-2121. Uncontained spills should be reported to 874-2618. 6. Concentrated acids and bases are ALWAYS handled in a fume hood, with the door below face level. 7. Wastes are ALWAYS stored in the lab in which they are generated (i.e. acetone waste in the fluorometer lab, autoanalyzer waste in the instrument lab, etc.). Do not transport wastes from one lab to another. All wastes must have a University of Rhode Island (URI) Safety and Health label on them. 8. Liquid wastes must have secondary containment adequate for the full volume of waste. 9. When waste containers are nearing full (~ 80% for high volume wastes or 90% for low volume wastes), coordinate with Linda Green or Elizabeth Herron to have them removed. Be sure all waste containers are properly and completely labeled at all times. 10. Whenever possible use a cart to transport water samples and/or reagents. 11. All chemicals must be properly labeled and stored at all times. Hazardous labels must indicate in English what harm the chemical represents (ie corrosive). 12. Liquid chemicals can not be stored above eye level. 13. If you have any safety or environmental questions, call URI Safety and Risk at 874-2618. 14. All URI Watershed Watch (WW) staff including student technicians are required to successfully complete URI’s “Environmental Awareness and Initial Waste Training” course within their first 6 months of employment. Subsequently, all URIWW staff including student technicians are required to successfully complete URI’s annual refresher course: “Prudent Practices and Laboratory Waste Management”. Certificates attesting to successful completion are posted in URIWW laboratories. General Laboratory Safety SOP 001 2 of 2 S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 001 Safety-mod.doc Standard Operating Procedure 001a Date: 5/99 University Safety and Waste Revision: 2 Author: URI Safety & Handling Document Risk (downloaded 6/24/09) University of Rhode Island Watershed Watch UNIVERSITY OF RHODE ISLAND DEPARTMENT OF SAFETY & RISK MANAGEMENT STANDARD OPERATING PROCEDURE SUBJECT: Hazardous Materials & Chemical Waste Number: 99-5-9 POLICY: This policy provides for the disposal of hazardous materials and chemical waste in accordance with all Federal and State regulations. APPLICABILITY: Faculty, Staff, Employees, Students, Guests. This policy applies to URI Kingston Campus, Narragansett Bay Campus (GSO), W. Alton Jones Campus, and Providence CCE Campus. Further, it applies to all departments and organizations on each of these campuses. All laboratory chemicals, oil-based paint, paint solvents, photographic chemicals, preservatives used for biological specimens, and any hazardous waste material listed or identified in Title 40 Code of Federal Regulations, Part 261, or any materials which exhibits the characteristics of ignitability, corrosivity, reactivity, or toxicity is covered by this policy. PURPOSE: To provide for the proper disposal of hazardous materials and chemical waste according to Federal and State hazardous waste disposal regulations. Failure to comply may result in civil, criminal, or administrative penalties for the University. PROCEDURE: Federal law requires that once a chemical is declared to be hazardous chemical waste, it must be removed from the campus within ninety (90) days. The University of Rhode Island is not permitted to treat or dispose of hazardous waste locally. It is illegal to dispose of hazardous chemical waste by evaporation, disposal down the sink drain to the sewer, or throwing it in the trash. Personnel from the Department of Safety and Risk Management collect, transport, and store hazardous chemical waste prior to final disposal by a licensed hazardous materials vendor. All faculty must establish laboratory waste management programs for the laboratories they teach. The waste should be collected at the end of each class and each student must be made responsible for disposing of the waste material to the appropriate containers. Principal Investigators are responsible for the waste generated in the research laboratories. A copy of the procedure that the Chemistry Department uses to instruct its teaching assistants is attached to this policy. Labels are available from the Department of Safety & Risk Management. Departments are encouraged to share chemicals to reduce waste. Buy the minimum amount required to reduce acquisition and disposal costs. This will insure that you have fresh reagents. If you have good quality chemicals that you wish to donate, you may contact Andy Clapham, the Chemistry Department stockroom manager, at 874-5084 (e-mail: [email protected]). URI Lab Waste Policy SOP 001a S:\WW\awwword\LABPROC\all QAPPs\LABQAPPs\QAPP Rev5 -0609\SOPs\SOP 001a - URI Waste Policy.doc SUBJECT: Hazardous Materials and Chemical Waste POLICY NO. 99-5-9 PAGE 2 The Department of Safety and Risk Management picks up waste chemicals from individual laboratories. Please call the Hazardous Materials and Chemical Waste Unit (874-2618) to arrange a pick- up. In the event of an emergency, please call S&RM’s main office number (874-2618). If you need to leave a voice mail, please speak clearly leaving your name, phone number, building name, room number, and a brief description of the waste to be picked up. If you have more than five (5) containers, the attached “Request for Hazardous Waste Disposal” form must be faxed in advance to the Department of Safety and Risk Management (Fax Number: 789-5126). All containers of chemicals to be picked up MUST be accompanied by a completed, typed form. ALL CONTAINERS MUST HAVE A PROPER LABEL. Responsibility for establishing the identity of any unknown materials rests with the department wishing to dispose of it. “Unknowns” may cost the University in excess of $100 for each analysis. Costs for unknowns may be charged back to departments in the future. Other Considerations: · For removal of radioactive materials, please contact the Radiation Safety Office at 874-6126. · For removal of infectious and biological waste (also called biohazard or medical waste), including broken or intact hypodermic needles or syringes that are contaminated by chemicals or that have been used in chemical laboratories, please call the Department of Safety and Risk Management (874-2618). · For disposal of potentially explosive materials such as picric acid, silanes, nitro compounds, and ethers, contact the Department of Safety and Risk Management (874-2618) for assistance. · Disposal of all hazardous chemicals down the
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