Soil Organic Carbon

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Soil Organic Carbon Food and Agriculture Organization of the United Nations Standard operating procedure for soil organic carbon Tyurin spectrophotometric method • ••••••• • • GWBAL'soIL PARTNERSHIP Standard operating procedure for soil organic carbon Tyurin spectrophotometric method Food and Agriculture Organization of the United Nations Rome, 2021 Required citation: FAO. 2021. Standard operating procedure for soil organic carbon: Tyurin spectrophotometric method. Rome. The designations employed and the presentation of material in this information product do not imply the expression of any opinion whatsoever on the part of the Food and Agriculture Organization of the United Nations (FAO) concerning the legal or development status of any country, territory, city or area or of its authorities, or concerning the delimitation of its frontiers or boundaries. 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Global Soil Laboratory Network GLOSOLAN-SOP-16 GLOSOLAN SOIL ORGANIC CARBON Version number : 01 Page 1 of 18 Tyurin spectrophotometric method Effective date : 13 January 2021 SOIL ORGANIC CARBON Tyurin spectrophotometric method VERSION HISTORY N° Date Description of the modification Type of modification 01 13 January 2021 All comments by RESOLANs and Finalization of the SOP reviewers to the draft SOP were addressed 02 03 Modify by Revision Approval date Validated date GLOSOLAN SOP Tech. W.G. Leader: Elena Shamrikova, Russian By the Review Panel 13 January 2021 13 January 2021 Federation Global Soil Laboratory Network GLOSOLAN-SOP-16 GLOSOLAN SOIL ORGANIC CARBON Version number : 01 Page 2 of 18 Tyurin spectrophotometric method Effective date : 13 January 2021 Contents 1. Brief introduction to soil organic matter (SOM) ........................................................................................... 3 2. Scope and field of application ..................................................................................................................... 3 3. Principle ...................................................................................................................................................... 3 4. Apparatus ................................................................................................................................................... 5 5. Materials ..................................................................................................................................................... 6 6. Health and safety ........................................................................................................................................ 7 6.1. Personnel safety ...................................................................................................................................... 7 6.2. Chemical hazard ...................................................................................................................................... 7 6.3. Soil sampling and storage ....................................................................................................................... 7 7. Sample preparation .................................................................................................................................... 8 8. Procedure ................................................................................................................................................... 8 8.1. Preparation of standards curve................................................................................................................ 8 8.2. Measuring the absorbance of calibration solutions and estimating the coefficients of the calibration function ........................................................................................................................................................... 9 8.3. Preparation of soils samples for measurements .................................................................................... 10 9. Calculation ................................................................................................................................................ 10 10. Quality assurance/Quality control ............................................................................................................. 11 10.1. Operational control of the intra-laboratory error of the measurement results using quality control samples ........................................................................................................................................................ 11 10.2. Operational control of the intra-laboratory error of the measurement results using another (control) method ......................................................................................................................................................... 12 10.3. Algorithm for controlling intra-laboratory precision ............................................................................... 12 Appendix I – Estimation of the coefficient taking into account incomplete oxidation of carbon in organic compounds in the Tyurin method ..................................................................................................................... 14 Appendix II – Preparation of saline samples .................................................................................................... 16 Appendix III – Acknowledgements ................................................................................................................... 17 Appendix IV – List of authors ............................................................................................................................ 17 Appendix V – Contributing laboratories ............................................................................................................ 18 Modify by Revision Approval date Validated date GLOSOLAN SOP Tech. W.G. Leader: Elena Shamrikova, Russian By the Review Panel 13 January 2021 13 January 2021 Federation Global Soil Laboratory Network GLOSOLAN-SOP-16 GLOSOLAN SOIL ORGANIC CARBON Version number : 01 Page 3 of 18 Tyurin spectrophotometric method Effective date : 13 January 2021 1. Brief introduction to soil organic matter (SOM) Soil organic matter (SOM) is one of the most important components of soil formation, being a combination of specific and non-specific organic compounds. SOM is formed during the decomposition and humification of biogenic residues (Semenov and Kogut, 2015). The chemical structure of soil organic matter is complex and not fully known. Traditionally, the SOM content in soils is estimated by determining the concentration of carbon associated with organic compounds (Corg) and multiplying Corg by a factor of 1.724. The value of the coefficient was established from the condition that Carbon makes up nearly 58% of SOM. However, the carbon content of SOM may vary depending on the type of soil. Consideration of the possible variation in the organic matter composition predicts a range
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