DOCSLIB.ORG

Alkaloids in Erythrina by UPLC-ESI-MS and in Vivo Hypotensive Potential of Extractive Preparations

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Alkaloids in Erythrina by UPLC-ESI-MS and in Vivo Hypotensive Potential of Extractive Preparations Hindawi Publishing Corporation Evidence-Based Complementary and Alternative Medicine Volume 2015, Article ID 959081, 12 pages http://dx.doi.org/10.1155/2015/959081 Research Article Alkaloids in Erythrina by UPLC-ESI-MS and In Vivo Hypotensive Potential of Extractive Preparations Liara Merlugo,1,2 Marí C. Santos,2 Liane S. Sant’Anna,1,3 EversonW.F.Cordeiro,2 Luiz A. C. Batista,2 Silvia T. S. Miotto,4 Cássia V. Garcia,5 Cleci M. Moreira,1,3 and Andreas S. L. Mendez1,5 1 Programa de Pos-graduac´ ¸ao˜ em Bioqu´ımica, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil 2Laboratorio´ de Desenvolvimento e Controle de Qualidade de Medicamentos, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil 3Laboratorio´ de Fisiologia Cardiovascular, Universidade Federal do Pampa, BR 472 Km 585, Caixa Postal 118, 97508-000 Uruguaiana, RS, Brazil 4Departamento de Botanica,ˆ Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil 5Faculdade de Farmacia,UniversidadeFederaldoRioGrandedoSul,AvenidaIpiranga2752,90610-000PortoAlegre,RS,Brazil´ Correspondence should be addressed to Andreas S. L. Mendez; [email protected] Received 21 April 2015; Revised 19 July 2015; Accepted 28 July 2015 Academic Editor: Nunziatina De Tommasi Copyright © 2015 Liara Merlugo et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Erythrina species are used in popular medicine as sedative, anxiolytic, anti-inflammatory, and antihypertensive. In this work, we investigated the chemical composition of extracts obtained from leaves of E. falcata and E. crista-galli.Thehypotensivepotential of E. falcata and the mechanism of action were also studied. The extracts were obtained by maceration and infusion. The total content of phenolic compounds and flavonoids was estimated by spectrophotometric methods. The chemical constituents were studied performing a chromatographic analysis by UPLC-ESI-MS. For in vivo protocols, blood pressure and heart rate were measured by the invasive hemodynamic monitoring method. Different concentrations of extracts and drugs such as L-NAME, losartan, hexamethonium, and propranolol were administrated i.v. The results of total phenolic contents for E. falcata and E. crista- galli were 1.3193–1.4989 mgGAE/mL for maceration and 0.8771–0.9506 mgGAE/mL for infusion. In total flavonoids, the content was 7.7829–8.1976 mg RE/g for maceration and 9.3471–10.4765 RE mg/g for infusion. The chemical composition was based on alkaloids, suggesting the presence of erythristemine, 11-methoxyglucoerysodine, erysothiopine, 11-hydroxyerysodine-glucose, and 11-hydroxyerysotinone-rhamnoside. A potent dose-dependent hypotensive effect was observed for E. falcata, which may be related to the route of -adrenergic receptors. 1. Introduction mechanism of action could have a vital role in the discovery of new products as therapeutic agents. Hypertension is a global health problem whose increased The genus Erythrina (Fabaceae) consists of 110 species, incidence can lead to the development of many chronic distributed in the tropical regions of America, Africa, Asia, diseases, as well as collaborating in the progression of chronic and Oceania [3, 4]. Phytochemical studies of its differ- kidney disease, and contribute to cardiovascular morbidity ent species showed a chemical constitution by alkaloids, and mortality [1]. Although there is a wide range of anti- flavonoids, pterocarpans, and cumarines [5–9]. Species as E. hypertensive drugs used in the control of hypertension, a velutina, E. mulungu, E. crista-galli,andE. falcata demon- successful treatment becomes even harder to achieve [2], so strated the predominance of alkaloids and flavonoids in the knowledge of medicinal plants, their composition, and their composition [10–14]. The alkaloids present in Erythrina 2 Evidence-Based Complementary and Alternative Medicine species have attracted much attention from researchers, since were analysed in a spectrophotometer Perkin Elmer UV-VIS they represent the main source of the tetracyclic alkaloids Lambda 35 (Norwalk, CT, USA) at 750 nm. The total phenolic of Erythrina type and have similar activities of the curare, content was expressed in milligrams of gallic acid equivalent causing muscle paralysis [15]. per mL of sample (mgGAE/mL). In popular medicine, these species are used for the treat- ment of respiratory diseases, as well as anxiolytic, sedative, 2.4. Total Flavonoid Content. The total flavonoid content was anti-inflammatory, and antihypertensive [11, 16]. Scientific determined by the method described by Chang et al. [23] studies showed different activities for these species such with some modifications. In a 25 mL flask 500 Lofsample as antibacterial, antifungal, and anti-inflammatory [17–19]. and 500 L of AlCl3 0.5% were added and the volume was Lehman [20] tested alcoholic extracts of E. americana and completed with water. It was incubated for 40 minutes at proved its hypotensive effect in rats, rabbits, and dogs. roomtemperatureandprotectedfromlightandreadingwas Alkaloids obtained from this species also demonstrated done in UV-VIS spectrophotometer Lambda 35 Perkin Elmer hypotensive activity [21]. (Norwalk, CT, USA) at a wavelength of 415 nm. The results The present study aimed to investigate the chemical were expressed in mg/g equivalents of rutin. composition of the species E. falcata and E. crista-galli by UPLC-ESI-MS and to evaluate the hemodynamic parameters 2.5. UPLC-MS. For the protocols a reverse-phase system was performed, using the following conditions: fast C18 analytical in rats, purposing to elucidate the involved mechanism of × action for E. falcata extracts. column Shim-pack XR-ODS (50 2 mm, 2.1 m); mobile phase consisted of acetonitrile : methanol 4 : 1 (v/v) and water with 0.1% formic acid (pH 3.0) with a flow rate of 0.2 mL/min; 2. Materials and Methods DAD detection at 340 nm; injection volume of 5.0 L. The elution gradient was as follows: 0% solvent A (0–2.5 min), 2.1. Plant Material. Erythrina crista-galli was collected in 0–5% A (2.5–7.5min), 5–10% A (7.5–10 min), 10–15% A (10– May 2013 in Uruguaiana, RS (Brazil). Erythrina falcata was 12.5 min), 15–20% A (12.5–15 min), 20–25% A (15–17.5 min), collectedinPortoAlegre,RS(Brazil),inFebruary2014.The 25% A (17.5–20 min), 25–60% A (20–27.5 min), 60% A (27.5– materials were identified and a voucher specimen of each 30 min), and 60–0% A (30–35 min). (Erythrina crista-galli,ICN183940;Erythrina falcata Benth., Chromatographic analyses were performed using an ICN 177665) was deposited at the ICN Herbarium (Instituto Acquity UPLC system (Waters Co., MA, USA) equipped with de Biociencias,ˆ UFRGS, Avenida Bento Gonc¸alves, 9500, binary solvent delivery system and autosampler. The DAD- Predio´ 43433, CEP 91501-970, Porto Alegre, RS, Brazil). UVandmassspectrometerEMQ-TOFMicro-Micromass (Waters Co., MA, USA) detectors were employed, with 2.2. Extracts Preparation. Leaves were selected and dried MassLynx v. 4.1 data acquisition software. ∘ at a temperature of 40 Cforfivedays.Afterthat,they The mass spectrometry analyses were performed in were grounded and submitted to exhaustive extraction pro- positive-ion mode and operated according to the following cesses by maceration using 40% ethanol as solvent (1 : 10, conditions: collision energy 4.0 eV; temperature of electro- ∘ ∘ plant : solvent) and by infusion using distilled water at a spray source and desolvation gas 100 Cand120C, respec- ∘ temperature of 100 C (1 : 15, plant : solvent). tively; capillary voltage 3000 V; sample cone 40 V; extraction For chromatographic assays, extracts obtained by mac- cone 3.0 V; N2 wasusedasthenebulizing.Massspectrawere eration process of both species were used. To reduce the recorded by using full scan mode in the range of m/z 200–800. impurities, extracts passed through a liquid-liquid extraction method by affinity solvents with increasing polarity (hex- 2.6. Hypotensive Activity and Mechanism of Action from ane, dichloromethane, ethyl acetate, and methanol) and for E. falcata Extracts further analysis they were filtered on a membrane of 0.45 2.6.1. Animals. Three-month-old male Wistar rats, weighing micrometers. around 300 g and purchased from the animal house of the For biological analyses macerations were used and infu- Universidade Federal de Santa Maria, were used. They were sions were obtained from E. falcata, which were oven-dried kept in cages with water available ad libitum, maintaining the and reduced to powder for subsequent redilution in 0.9% ∘ temperature controlled at 20–22 C with dark/light cycle of 12 saline solution for administration. hours. The experimental protocols followed the International 2.3. Total Phenolic Content. The total phenolic content was Principles for Research Involving Animals (Geneva) (Brazil- determined by Folin-Ciocalteu colorimetric method with ian legislation by law number 11.794/2008 (procedures for the some modifications [22]. For the analyses, aliquots of 100 L scientific use of animals), Decree 24.645/34 (animal rights), of the samples and standard solutions were transferred to approved by the Ethics Committee on Animal Use (CEUA), test tubes, where 500 L of Folin-Ciocalteu reagent and 6 mL Universidade Federal do Pampa (Protocol 030/2013)). of distilled water were added. Then, they were agitated and left at rest for 1 minute. After alkalizing the medium, 2mL 2.6.2. Hemodynamic Parameters. Rats were anesthetized of Na2CO3 solution 15% was added, and the volume was with urethane (1.4 mg/kg, i.p.) and submitted to surgery for completed to 10 mL with distilled water. After 30 minutes at catheterization of the carotid artery (to measure the hemody- room temperature and being protected from light, samples namic parameters) and the jugular vein (for administration of Evidence-Based Complementary and Alternative Medicine 3 the extracts and drugs).
Load more

Recommended publications
  • Universidad Técnica Particular De Loja Àrea
    UNIVERSIDAD TÉCNICA PARTICULAR DE LOJA La Universidad católica de Loja ÀREA BIOLÓGICA TITULO DE BIOQUÍMICA Y FARMACIA Obtención de extractos, aislamiento y caracterización de metabolitos secundarios de la especie Erythrina smithiana. TRABAJO DE TITULACIÓN AUTOR: Aguilar Jaramillo, Hermes Leonardo DIRECTOR: Romero Benavides, Juan Carlos, Ing. LOJA – ECUADOR 2015 Esta versión digital, ha sido acreditada bajo la licencia Creative Commons 4.0, CC BY-NY- SA: Reconocimiento-No comercial-Compartir igual; la cual permite copiar, distribuir y comunicar públicamente la obra, mientras se reconozca la autoría original, no se utilice con fines comerciales y se permiten obras derivadas, siempre que mantenga la misma licencia al ser divulgada. http://creativecommons.org/licenses/by-nc-sa/4.0/deed.es Septiembre, 2015 APROBACIÓN DEL DIRECTOR DEL TRABAJO DE TITULACIÓN Ingeniero. Juan Carlos Romero Benavides. DOCENTE DE LA TITULACIÓN De mi consideración: El presente trabajo de titulación: “Obtención de extractos, aislamiento y caracterización de metabolitos secundarios de la especie Erythrina smithiana con actividad antiparasitaria”, realizado por Hermes Leonardo Aguilar Jaramillo, ha sido orientado y revisado durante su ejecución, por cuanto se aprueba la presentación del mismo. Loja, septiembre de 2015 f)____________________________ Ing. Romero Benavides Juan Carlos C.I. 1103018477 ii DECLARACIÓN DE AUTORÍA Y CESIÓN DE DERECHOS. Yo, Hermes Leonardo Aguilar Jaramillo, declaro ser autor del presente trabajo de titulación: “Obtención de extractos, aislamiento y caracterización de metabolitos secundarios de la especie Erythrina smithiana con actividad antiparasitaria”, de la titulación de Bioquímica y Farmacia, siendo el Ing. Juan Carlos Romero Benavides, director del presente trabajo; y eximo expresamente a la Universidad Técnica Particular de Loja y a sus representantes legales de posibles reclamos o acciones legales.
    [Show full text]
  • Suzane Virtuoso Estudo Fitoquímico E Biológico Das
    SUZANE VIRTUOSO ESTUDO FITOQUÍMICO E BIOLÓGICO DAS CASCAS DE Erythrina velutina Willd. - FABACEAE (LEGUMINOSAE - PAPILIONOIDEAE) Dissertação apresentada como requisito parcial à obtenção do grau de Mestre em Ciências Farmacêuticas, curso de Pós - Graduação em Ciências Farmacêuticas, Setor de Ciências da Saúde, Universidade Federal do Paraná. Orientador: Prof. Dr. Obdulio Gomes Miguel Co-Orientadora: Profª. Dra. Marilis Dallarmi Miguel CURITIBA 2005 This document has been created with a DEMO version of PDF Create Convert (http://www.equinox-software.com/products/pdf_create_convert.html) To remove this message please register. Pelo amor, união, apoio e paciência em todos os momentos, dedico este trabalho à minha preciosa família Luiz, Leonilda e Luiz Aurélio. ii This document has been created with a DEMO version of PDF Create Convert (http://www.equinox-software.com/products/pdf_create_convert.html) To remove this message please register. AGRADECIMENTOS Ao meu orientador Prof. Dr. Obdulio Gomes Miguel pela confiança, paciência, estímulo à pesquisa e pelo seu modo especial de ver a vida e as pessoas. À minha co-orientadora Profª. Drª. Marilis Dallarmi Miguel pelo dinamismo, entusiasmo, prontidão em ajudar e encaminhar e, pela confiança depositada. À Universidade Estadual do Oeste do Paraná pela liberação em afastamento parcial, à coordenadora e vice do curso de Farmácia, Nadir Marcondes e Nereida Gioppo pelo apoio e compreensão, a todos os (as) professores (as) e funcionários (as) do curso de Farmácia, em especial aos amigos da Farmácia Escola pelo excelente relacionamento de trabalho, ajuda, momentos alegres e ao meus queridos e compreensivos alunos. Aos professores do Programa de Pós-graduação em Ciências Farmacêuticas da UFPR, que direta ou indiretamente participaram da evolução do trabalho e aos funcionários, especialmente Regina, Hilda e Geni pela ajuda, cafés e bons momentos, também ao Dr.
    [Show full text]
  • A Phytochemical and Ethnopharmacological Review of the Genus Erythrina
    16 A Phytochemical and Ethnopharmacological Review of the Genus Erythrina João X. de Araújo-Júnior, Mariana S.G. de Oliveira, Pedro G.V. Aquino, Magna S. Alexandre-Moreira and Antônio E.G. Sant’Ana Universidade Federal de Alagoas Brazil 1. Introduction Considered in acient times as a connection to the divine, the use of this medicinal plant is as old as human civilization itself. Whole nations dominated its secrets, often associated with magic and religious rites, searching in nature’s resources to improve life conditions, and increase chances of survival (Herbarium, 2008). In 1978, the World Health Organization (WHO) recognized folk medicine and its beneficial effects to health, during the Alma Ata conference, which published in 1985 that approximatly 80% of the global population, resorted to traditional medicine as their primary health treatment (Herbarium, 2008). Medicinal plants have been used as a means of curing or preventing diseases, now called phytotherapy, in all regions of the world, with regional variations due to the influence of cultural characteristics of the population, as well as its flora, soil and climate (Lewinsohn, 2003). Since the nineteenth century, humanity discovered the endless and diverse therapeutic arsenal present in medicinal plants, due to the discovery of active substances that in their natural state or after chemical transformation showed biological activity, and often already confirmed by popular use and/or proven scientifically (Miguel & Miguel, 2004). According to Yamada (1998) it is necessary to carry out more studies and to propagate medicinal plant utilization as a way to diminish the costs of public health programs since the utilization of these plants may constitute a very useful therapeutic value due their efficacy coupled with low operating costs and the relative ease of obtaining the plants (Matos, 1994).
    [Show full text]
  • Contribuição Ao Conhecimento De Erythrina Velutina Willd. (Fabaceae-Faboideae): Uma Abordagem Farmacobotânica, Química E Farmacologica
    UNIVERSIDADE FEDERAL DA PARAÍBA LABORATÓRIO DE TECNOLOGIA FARMACÊUTICA “PROF. DELBY FERNANDES MEDEIROS” PROGRAMA DE PÓS-GRADUAÇÃO EM PRODUTOS NATURAIS E SINTÉTICOS BIOATIVOS Contribuição ao Conhecimento de Erythrina velutina Willd. (Fabaceae-Faboideae): Uma abordagem Farmacobotânica, Química e Farmacologica INGRID CHRISTIE ALEXANDRINO RIBEIRO DE MELO João Pessoa - PB 2011 MELO, I. C. A. R Contribuição ao Conhecimento de Erythrina velutina... INGRID CHRISTIE ALEXANDRINO RIBEIRO DE MELO Contribuição ao Conhecimento de Erythrina velutina Willd. (Fabaceae-Faboideae): Uma abordagem Farmacobotânica, Química e Farmacologica Dissertação apresentada ao Programa de Pós- graduação em Produtos Naturais e Sintéticos Bioativos do Centro de Ciências da Saúde, da Universidade Federal da Paraíba, em cumprimento às exigências para a obtenção do título de Mestre em Produtos Naturais e Sintéticos Bioativos. Área de Concentração: Farmacoquímica. Orientadora: Profª. Drª. Maria de Fátima Agra Co-Orientador: Prof. Dr. Eduardo de Jesus Oliveira João Pessoa - PB 2011 2 MELO, I. C. A. R Contribuição ao Conhecimento de Erythrina velutina... INGRID CHRISTIE ALEXANDRINO RIBEIRO DE MELO Contribuição ao Conhecimento de Erythrina velutina Willd. (Fabaceae-Faboideae): Uma abordagem Farmacobotânica, Química e Farmacologica Dissertação aprovada em 25 de Fevereiro de 2011 COMISSÃO EXAMINADORA: _________________________________ Profª.Drª. Maria de Fátima Agra (Universidade Federal da Paraíba) Orientadora _________________________________ Prof.Dr. Rivete Silva de Lima (Universidade Federal da Paraíba) Examinador Externo _________________________________ Profª.Drª. Márcia Regina Piuvezam (Universidade Federal da Paraíba) Examinador Interno 3 MELO, I. C. A. R Contribuição ao Conhecimento de Erythrina velutina... Á minha família, especialmente aos meus pais Maria José e Cristóvão, pelo apoio e motivação e ao meu namorado Leonardo Antunes Trindade, por sua paciência e dedicação.
    [Show full text]
  • Seed Germination and Production of Erythrina Mulungu and Erythrina Velutina Plantlets
    American Journal of Plant Sciences, 2014, 5, 535-540 Published Online March 2014 in SciRes. http://www.scirp.org/journal/ajps http://dx.doi.org/10.4236/ajps.2014.55068 Seed Germination and Production of Erythrina mulungu and Erythrina velutina Plantlets Ana Maria Soares Pereira1*, Verena Trevizani Alves Souza1, Juliana da Silva Coppede1, Suzelei de Castro França1, Bianca Waléria Bertoni1, Ana Valéria Vieira de Souza2 1Department of Biotechnology, University of Ribeirão Preto, Ribeirão Preto, Brazil 2Embrapa Semiárido, Centro de Pesquisa Agropecuaria do Tropico Semi-Arido, Petrolina, Brazil Email: *[email protected] Received 28 November 2013; revised 17 January 2014; accepted 23 February 2014 Copyright © 2014 by authors and Scientific Research Publishing Inc. This work is licensed under the Creative Commons Attribution International License (CC BY). http://creativecommons.org/licenses/by/4.0/ Abstract Species of the genus Erythrina are frequently used as ornamental plants and in projects to restore degraded areas. Also, extracts from its shoots and stems are popularly used as a herbal medicine with validated activity on the central nervous system. The objective of this study was to evaluate E. mulungu and E. velutina seed germination and seedling development potential aiming commercial scale production of those species. Seeds stored for one or twelve months at 10˚C ± 2˚C were sown in sand, soil or Plantmax® substrates and evaluated for germination and seedling development. Subsequently, seedlings sprouted in Plantmax® were transferred to polyethylene bags and kept in greenhouse, under direct sunlight for plant development (plant height, stem diameter and root length pivoting) evaluations. Four-month-old plantlets were transplanted to the field and after a period of one year the collar diameter and shoot height (of each plant were measured.
    [Show full text]
  • GÉNERO Erythrina: ACTUALIDAD EN LA INVESTIGACIÓN Y PERSPECTIVAS DE DESARROLLO CIENTÍFICO
    GÉNERO Erythrina: ACTUALIDAD EN LA INVESTIGACIÓN Y PERSPECTIVAS DE DESARROLLO CIENTÍFICO Luisa Fernanda Velásquez Holguín Diego Fernando Montoya Yepes Ángel Arturo Jiménez Rodríguez Walter Murillo Arango Jonh Jairo Méndez Arteaga UNIVERSIDAD DEL TOLIMA Género Erythrina : actualidad en la investigación y perspectivas de desarrollo científico / Luisa Fernanda Velásquez Holguín … [et al.] -- 1ª. ed. -- Universidad del Tolima, 2019. 156 p. : il., gráficas, tablas Contenido: Generalidades del género Erythrina – Tendencias de investigación en la última década del género Erythrina – Chachafruto : alimento del futuro. Incluye bibliografía ISBN: 978-958-5569-03-4 1. Género Erythrina – Botánica 2. Género Erythrina – Farmacología 3. Género Erythrina – Agricultura 4. Chachafruto I. Título II. Montoya Yepes, Diego Fernando III. Jiménez Rodríguez, Ángel Arturo IV. Murillo Arango, Walter V. Méndez Arteaga, Jonh Jairo VI. Velásquez Holguín, Luisa Fernanda 583.74 G326 © Sello Editorial Universidad del Tolima, 2019 © Luisa Fernanda Velásquez Holguín, Diego Fernando Montoya Yepes, Ángel Arturo Jimenez Rodríguez, Walter Murillo Arango, Jonh Jairo Méndez Arteaga, Primera edición digital ISBN electrónico: 978-958-5569-03-4 Número de páginas: 132 Ibagué-Tolima Facultad de Ciencias Básicas Género Erythrina: actualidad en la investigación y perspectivas de desarrollo científico Grupo de Investigación en Productos Naturales -GIPRONUT Universidad del Tolima [email protected] [email protected] Impresión, diseño y diagramación: PROVEER PRODUCTOS Y SERVICIOS
    [Show full text]
  • Monografia Da Espécie Erythrina Mulungu (Mulungu)
    MINISTÉRIO DA SAÚDE MONOGRAFIA DA ESPÉCIE ERYTHRINA MULUNGU (MULUNGU) Organização: Ministério da Saúde e Anvisa Fonte do Recurso: Ação 20K5 (DAF/ SCTIE/ MS)/2013 Brasília 2015 FICHA DE CATALOGAÇÃO 2 3 LISTA DE ILUSTRAÇÕES Figura 1: E. mulungu. Figura 2: Descrição macroscópica e microscópica das cascas de E. mulungu. Figura 3: Descrição microscópica das cascas de E. mulungu. Figura 4: Descrição microscópica do pó das cascas de E. mulungu. Figura 5: Estruturas químicas dos principais compostos produzidos pela espécie E. mulungu. 4 LISTA DE TABELAS Tabela 1 - Patentes solicitadas para a espécie Erythrina mulungu no banco de dados WIPO. Tabela 2 - Patentes solicitadas para a espécie Erythrina mulungu no banco de dados EPO. Tabela 3 - Patentes solicitadas para a espécie Erythrina mulungu no banco de dados JPO. 5 ABREVIATURAS ANVISA – Agência nacional de Vigilância sanitária DL50 – Dose Letal 50% EPO - European Patent Office I.C.V. -intracerebrovascular I.P. - intraperitoneal I.V - intravascular INPI - Instituto Nacional da Propriedade Industrial JPO - Japanese Patente Office NMDA - N-metil-D-aspártico OMS – Organização Mundial da Saúde PTZ - pentilenotetrazol RDC - Resolução da Diretoria Colegiada RMN – Ressonância Magnética Nuclear SNC – Sistema Nervoso Central USPTO - United States Patent and Trademarks V.O. – via oral WIPO - World Intellectual Property Organization 6 SUMÁRIO 1 IDENTIFICAÇÃO .................................................................................................... 9 1.1 NOMENCLATURA BOTÂNICA ....................................................................
    [Show full text]
  • Effect of Erythrina Mulungu on Anxiety During Extraction of Third Molars
    Med Oral Patol Oral Cir Bucal. 2014 Sep 1;19 (5):e518-24. Erythrina mulungu in dental anxiety control Journal section: Oral Surgery doi:10.4317/medoral.19511 Publication Types: Research http://dx.doi.org/doi:10.4317/medoral.19511 Effect of Erythrina mulungu on anxiety during extraction of third molars Maria-Luisa Silveira-Souto 1, Carla-Rocha São-Mateus 1, Liane-Maciel de Almeida-Souza 1, Francisco-Carlos Groppo 2 1 Department of Dentistry of the Federal University of Sergipe, Aracaju, Sergipe, Brazil 2 Department of Physiological Sciences, Piracicaba Dental School, University of Campinas,Piracicaba, São Paulo, Brazil Correspondence: Claudio Batista Street no number, Cidade Nova Aracaju, Sergipe, Brazil [email protected] Silveira-Souto ML, São-Mateus CR, de Almeida-Souza LM, Groppo FC. Effect of Erythrina mulungu on anxiety during extraction of third molars. Med Oral Patol Oral Cir Bucal. 2014 Sep 1;19 (5):e518-24. http://www.medicinaoral.com/medoralfree01/v19i5/medoralv19i5p518.pdf Received: 27/08/2013 Accepted: 25/12/2013 Article Number: 19511 http://www.medicinaoral.com/ © Medicina Oral S. L. C.I.F. B 96689336 - pISSN 1698-4447 - eISSN: 1698-6946 eMail: [email protected] Indexed in: Science Citation Index Expanded Journal Citation Reports Index Medicus, MEDLINE, PubMed Scopus, Embase and Emcare Indice Médico Español Abstract Objectives: The aim of the present study was to evaluate the effect of Erythrina mulungu on the control of dental anxiety in patients who had under gone bilateral extraction of asymptomatic, impacted mandibular third molars. Material and Methods: In a randomized, double-blind, crossover study, 30 healthy volunteers (5 men and 25 wom- en, over 18 years of age), received either 500mg of E.mulungu (Mulungu Matusa®) or 500 mg of placebo, p.o., one hour before surgical procedure.
    [Show full text]
  • Mulungu – Rainforest Anxiolytic
    16 Mulungu – Rainforest Anxiolytic Patocka Jiri Department of Radiology and Toxicology, Faculty of Health and Social Studies University of South Bohemia Ceske Budejovice Czech Republic 1. Introduction There are over one hundred species of plants of the genus Erythrina in the tropics and many of them are native to the American continent (Neill 1988). Erythrina species are known to produce alkaloids, flavonoids and terpenes (Garcia-Mateos et al., 1998). As with other species of Erythrina, alkaloids appear to be one of the main constituents mulungu (Parsons and Palframan, 2010). Mulungu (Erythrina mulungu) is a branched tree native to Brazil, growing in rain-forestry of Amazonia. Since its flowers are the same red color as coral, the plant is sometimes also called "flor de coral" and in English literature "coral tree". The designation Erythrina also includes the species Erythrina velutina, endemic to the semi-arid regions of Northeastern Brazil, and Erythrina mulungu, a plant native to Southern Brazil. In native herbal medicine, a leaf or bark decoction or tincture from mulungu has long been used in folk medicine due to their tranquilizing effects and as natural sedative. It is also anxiolytic and antibacterial (Garín-Aguilar et al., 2000). In both Brazil and Peru mulungu is used for epilepsy (Vasconcelos et al., 2007). Practitioners in the United States use mulungu to quiet hysteria from trauma or shock, as a mild sedative to calm the nervous system, to treat insomnia and promote healthy sleeping patterns. Anxiety disorders are among the most prevalent psychiatric diseases and mulungu offers novel therapy chance (Balbani et al., 2009; Patocka, 2009).
    [Show full text]
  • Erysothrine,An Alkaloid Extracted from Flowers of Erythrina Mulungu Mart
    Erysothrine,an alkaloid extracted from flowers of Erythrina mulungu Mart. ex Benth:Evaluating its anticonvulsant and anxiolytic potential Epppyilepsy & Behavior 23(2012)205-212 Presented by Pratthana Srichomphu 1st year master student Neurosciences Program Content Introduction & Rationale Objective MtMater ilials &Mthd& Methods Result & discussion Conclusions What is the seizure? Excitation Inhibition Inhibition Excitation Too much excitation Increased activation of NMDA receptors Increased inward Na+, Ca++ currents Too littl e in hibition Defective GABA A or GABA-B inhibition Defective activation of GABA neurons Defective intracellular buffering of calcium Defective inward CI-, outward K+ currents Classification of seizures Partial seizures Generalized seizures Standard Treatment of seizures More than 80% of patients with epilepsy can have their seizures controlled with medication Excitation Inhibitation Cl- Cl- Diazepam Chemical Convulsant ¾Pentylenetetrazol (PTZ) ¾Bicuculline GABA antagonist ¾Kainic acid Glutamate agonist ¾N-Methyl D-Aspartate (NMDA) Tonic – clonic seizure Chemical Convulsant Cl- Bicuculine Ca++ Ca++ Kainic acid NMDA PTZ Folk medicine AtiAnticonvu lsan t, ana lges ic, se dtidative, hypno tic Family: Fabaceae Genus:andErythrina hypotension properties Species: mulungu, cristi -galli Synonyms: Erythrina verna, Corallodendron mulungu Common Names: Mulungu, corticeira, murungu, muchocho, murungo, totocero, Lourenzi et al 1992,Rodrigues et al 2001 Hydroalcohoholic extract Analgesic Anticonvulsant Vasconcelos et al2007 The
    [Show full text]
  • A Systematic Updated Review of Scientifically Tested Selected Plants Used for Anxiety Disorders
    Botanics: Targets and Therapy Dovepress open access to scientific and medical research Open Access Full Text Article REVIEW A systematic updated review of scientifically tested selected plants used for anxiety disorders Ashutosh Sharma Abstract: The aim of this review is to provide a summary on multidisciplinary scientific Alexandre Cardoso-Taketa information obtained from medicinal plants used worldwide to treat anxiety, focusing on phar- Griselda García macological and clinical studies. The bibliographical investigation was carried out by consulting María Luisa Villarreal five peer-reviewed worldwide database publications for references, and patents. The information gathered on plants with attributed anxiolytic properties are presented as follows: (1) plant extracts Centro de Investigación en Biotecnología, Universidad Autónoma with anxiolytic properties evaluated in animal models; (2) plants with clinical trials; (3) identi- del Estado de Morelos, Cuernavaca, fied active compounds in plants that have been assayed in animal models; (4) mechanism of Mexico action of anxiolytic plant extracts and compounds; and (5) registered patents for anxiolytic plant preparations. We recorded 112 plant species belonging to 63 botanical families for which the For personal use only. anxiolytic properties had been tested in animal models. Eleven plant species to treat general anxiety disorders as well as eleven species to treat anxiety-associated conditions, had been documented by clinical trials. Thirty-three registers for active compounds belonging to five general types of secondary metabolites had also been recorded. The mechanism of action at the central nervous system level had been determined in 33 plant species, either in their extracts or isolated compounds. Forty-seven patent registrations for plant preparations to be used for the treatment of anxiety were included.
    [Show full text]
  • Larvicidal Efficacy of Plant Extracts and Isolated Compounds from Annonaceae and Piperaceae Against Aedes Aegypti and Aedes Albopictus Alzeir M
    Asian Pacific Journal of Tropical Medicine 2020; 13(9): 384-396 384 Review Article Asian Pacific Journal of Tropical Medicine journal homepage: www.apjtm.org doi:10.4103/1995-7645.290583 Impact Factor: 1.94 Larvicidal efficacy of plant extracts and isolated compounds from Annonaceae and Piperaceae against Aedes aegypti and Aedes albopictus Alzeir M. Rodrigues1,2, Victor Emanuel P. Martins3, Selene M. Morais4 1Departamento de Ensino, Instituto Federal de Educação, Ciência e Tecnologia do Ceará (IFCE), Rodovia CE 060, km 332, Vila Martins, 63.560- 000 Acopiara, Ceará, Brazil 2Programa de Pós-Graduação em Biotecnologia, RENORBIO, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, 60741-000 Fortaleza, Ceará, Brazil 3Instituto de Ciências Exatas e da Natureza, Universidade da Integração Internacional da Lusofonia Afro-Brasileira (Unilab), Campus das Auroras, Rua José Franco de Oliveira s/n, 62790-970 Redenção, Ceará, Brasil 4Departamento de Química, Universidade Estadual do Ceará, Avenida Doutor Silas Munguba, 1700, 60741-000 Fortaleza, Ceará, Brazil ABSTRACT 1. Introduction The Aedes (Ae.) aegypti and Ae. albopictus mosquitoes are vectors Dengue, chikungunya and Zika viruses are emerging arboviruses of epidemiologically relevant arboviruses in the public health in tropical countries such as Brazil, being spread mainly by Aedes context, such as the dengue, Zika and chikungunya viruses. Among (Ae.) aegypti and Ae. albopictus . Lately, the flow of people [1] [2] the alternatives to synthetic insecticides for the control of these between continents has increased the transmission of these vectors, the use of natural plant products deserves attention. This arboviruses, which has prompted numerous efforts to implement review summarizes findings on the larvicidal potential of plant control strategies, given the lack of effective vaccines against their extracts on Ae.
    [Show full text]