Human Activin RIIB Antibody

Monoclonal Mouse IgG2B Clone # 573107 Catalog Number: MAB339

DESCRIPTION Species Reactivity Human

Specificity Detects human Activin RIIB in direct ELISAs and Western blots. Source Monoclonal Mouse IgG Clone # 573107 2B Purification Protein A or G purified from hybridoma culture supernatant

Immunogen Mouse myeloma cell line NS0­derived recombinant human Activin RIIB Ser19­Thr134 Accession # CAA54671 Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.

APPLICATIONS Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website. Recommended Sample Concentration Flow Cytometry 2.5 µg/106 cells See Below

DATA

Flow Cytometry Detection of Activin RIIB in K562 Human Cell Line by Flow Cytometry. K562 human chronic myelogenous leukemia cell line was stained with Mouse Anti­Human Activin RIIB Monoclonal Antibody (Catalog # MAB339, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin­conjugated Anti­Mouse

IgG F(ab')2 Secondary Antibody (Catalog # F0101B).

PREPARATION AND STORAGE Reconstitution Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. Stability & Storage Use a manual defrost freezer and avoid repeated freeze­thaw cycles. l 12 months from date of receipt, ­20 to ­70 °C as supplied. l 1 month, 2 to 8 °C under sterile conditions after reconstitution. l 6 months, ­20 to ­70 °C under sterile conditions after reconstitution.

BACKGROUND Members of the TGF­β superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine­threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor­beta (TGF­beta) family members including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple Activin RIIB isoforms can also be generated.

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