Activin Receptor Type IIB (RIIB)

Catalog number A0856-10D Supplier United States Biological

Members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine-threonine kinase receptor, both of which are essential for signal transduction. Activin receptor type IIB is a type II receptor that mediates multiple signals for transforming growth factor-beta (TGF-beta) family members, including Activin, Nodal, Bmp7, Gdf1, Gdf3, Myostatin (Gdf8), and Gdf11. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated. Applications Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution . Western Blot: 1ug/ml using recombinant human Activin RIIB Fc Chimera. Optimal dilutions to be determined by the researcher. Storage and Stability Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Immunogen Recombinant protein corresponding to Ser19-Thr134 from human Activin Receptor Type IIB, expressed in NSO cells. Swiss/UniProt Accession # Q13705. Formulation Supplied as a lyophilized powder from PBS, pH 7.4, 5% trehalose. Reconstitute with 200ul sterile PBS. Purity Purified by Protein G affinity chromatography from ascites. Specificity Recognizes human Activin Receptor Type IIB. Shows ~5% crossreactivity with recombinant human Activin RIIA. Product Type Mab Source human Isotype IgG1 Grade Affinity Purified Applications E WB Crossreactivity Hu Storage -20°C