1 Novel Insights Into Karyotype Evolution and Whole Genome
bioRxiv preprint doi: https://doi.org/10.1101/099044; this version posted January 8, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC 4.0 International license. Novel insights into karyotype evolution and whole genome duplications in legumes Melissa M.L. Wong1, René E. Vaillancourt2, Jules S. Freeman2, Corey J. Hudson3, Freek T. Bakker4 , Charles, H. Cannon5, and Wickneswari Ratnam1 1School of Environment and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, Malaysia 2School of Biological Sciences, University of Tasmania, PB 55, Tasmania, Australia 3Tasmanian Alkaloids, Australia 4Biosystematics Group, Wageningen University, Wageningen, The Netherlands 5Center for Tree Science, The Morton Arboretum, USA ABSTRACT Legumes (family Fabaceae) are globally important crops due to their nitrogen fixing ability. Papilionoideae, the best-studied subfamily, have undergone a Whole Genome Duplication (WGD) around 59 million years ago. Recent study found varying WGD ages in subfamilies Mimosoideae and Caesalpinioideae and proposed multiple occurrences of WGD across the family based on gene duplication patterns. Despite that, the genome evolution of legume ancestor into modern legumes after the WGD is not well-understood. We aimed to study genome evolution at the subfamily level using gene-based linkage maps for Acacia auriculiformis and A. mangium (Mimosoideae) and we discovered evidence for a WGD event in Acacia. In additional to synonymous substitution rate (Ks) analysis, we used ancestral karyotype prediction to further corroborate this WGD and elucidate underlying mechanisms of karyotype evolution in Fabaceae.
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