Use of Polarising Microscope
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EXPERIMENT 6 USE OF POLARISING MICROSCOPE Structure_____________________________________________________ 6.1 Introduction 6.4 Optical Properties of Minerals Expected Skills Under Plane Polarised Light 6.2 Requirements Between Cross Nicol 6.5 Laboratory Exercises 6.3 Basic Concepts Parts and Functioning of Polarising Microscope 6.6 References Adjustments of Microscope 6.7 Learning Resources 6.1 INTRODUCTION In the previous two experiments, you have identified minerals based on their physical properties. Now in the next two experiments, you would learn to identify the same minerals based on their optical properties. The optical properties are studied under the polarising/petrological microscope, using transmitted light. So, prior to that get familiarised with a polarising microscope you will be using for mineral identification. You will be handling and working on polarising microscope for the first time. In this experiment, you will learn the use of polarising microscope for the study of minerals. In this experiment we will give a brief account of polarising microscope and its functioning in the identification of minerals. You will also learn about the optical properties used for the identification of minerals both under polarised light and cross nicol condition. With the help of these optical properties you will examine and identify the minerals in the subsequent Experiments 7 and 8. Expected Skills_______________________________________________ After performing this experiment, you should be able to describe polarising microscope; discuss functioning and parts of the polarising microscope; make necessary adjustments in the microscope for its usage; list optical properties of the minerals under plane polarised light, and ……………………………..…………………….…………………………………………………………………………………………………………………………………………….BGYCL-134 Crystallography, Mineralogy and Economic Geology: Laboratory list optical properties of the minerals in cross nicol condition. 6.2 REQUIREMENTS You need to have a polarising microscope in order to know its handling and use. You will get this microscope from your study centre. If the number of microscopes is limited in the study centre, you are advised to work in groups. You would also require thin section of the mineral. Requirements Polarising microscope Mineral thin sections Instructions: You are required to study Unit 4 Polarising Microscope of BGYCT-133 course (Crystallography, Mineralogy and Economic Geology) before performing this experiment. Bring this practical manual along with Block 3 Optical Mineralogy of BGYCT-133 course while attending the Practical Counselling session. 6.3 BASIC CONCEPTS A polarising microscope, also known as petrological microscope, is a type of microscope that uses polarised and transmitted light for study of optical properties of the crystalline material such as minerals and rocks. The polarising microscopes are of two types: transmitted light microscope used for the study of rocks and minerals; and reflected light microscope used for the study of opaque minerals, mostly ores. In this experiment, we will discuss about transmitted light polarising microscope in details. 6.3.1 Parts and Functioning of Polarising Microscope A transmitted light polarising microscope is different from other microscopes as it is equipped with two polars (i.e., nicol prisms) that are oriented at right angles to each other so that their polarisation directions are perpendicular to one another (Fig. 6.1 and 6.2). The lower polarising nicol is called as the ‘polariser’ and the upper as ‘analyser’ as it aids analysis. Light source located below the stage of the microscope is initially unpolarised. It first passes through the lower polariser (usually, just called the polariser), where it gets polarised in the 94 Experiment……………………………..…………….………………………………………………………………………………………………………………………………………………… 6 Use of Polarising Microscope manner that the light starts vibrating from (the users) right to left. These directions are referred to East (right) and West (left). This is called Plane Polarised Light (abbreviated as PPL). The analyser is similar to polariser, but is oriented at right angle to polariser. It has a polarisation direction exactly perpendicular to that of the lower polariser. These directions are usually referred to North-South (Fig. 6.2a and b). Fig. 6.1: Polarising microscope and important components. When the analyser is inserted in the microscope, without a thin section of mineral specimen then the field of view becomes dark, provided polariser and analyser must be at 90º to each other. Small difference in the angle will little amount of light to pass through and field of view will not be perfectly dark. In cross nicol condition the analyser receives light vibrating in an East-West direction from the polariser, but because of orientation cannot transmit it, as it is absorbed. The above arrangement of analyser to polariser is referred to as cross polars or cross nicol condition and is abbreviated as XPL. Let us discuss different parts of the polarising microscope and their functioning. Concave mirror: Near the base of the polarising microscope, there is a concave mirror which reflects the ordinary light upwards (Fig. 6.1). The source of light may be natural like sunlight or an artificial, for example, an electric bulb. 95 ……………………………..…………………….…………………………………………………………………………………………………………………………………………….BGYCL-134 Crystallography, Mineralogy and Economic Geology: Laboratory Polariser: The ordinary light which is initially unpolarised, becomes polarised after passing through the polariser. The light leaves the polariser and starts vibrating parallel to the short diagonal of the nicol prism. This light first passes through the lower polariser (also known as polariser), where it becomes polarised and vibrates from the users right to left. These directions are referred to as East (right) and West (left). Sub-stage Diaphragm(s): One or two diaphragms may be located below the stage. Function of the diaphragms is to reduce the area of light entering the thin section. (a) (b) Fig. 6.2: Polarising Microscope: a) Parts and functioning; and b) Optical path of transmitted light. Mineral Plate on Graduated Rotating Disc or Stage: The graduated rotatable disc, on which thin section of the mineral or rock is placed, lies in between the two nicols. The microscopic stage or disc is capable of rotation and can be locked at any point. It is employed for precise angular measurement. The light entering from the polariser is resolved into two vibration directions at right angles to each another. They are parallel to the vibration direction of the mineral. Hence, two rays leave the mineral plate. Objective lens: It is placed at lower end of the microscope tube. Its function is to produce a sharp and clear image. The light passing through a hole in 96 Experiment……………………………..…………….………………………………………………………………………………………………………………………………………………… 6 Use of Polarising Microscope the rotatable stage of the microscope enters the lower lens, called an objective lens. For mineralogical work, there are three objectives: low, medium and high power for image magnification as may be required. These objectives are mounted on nose piece and can be successively rotated into position. Microscope tube: The microscope is focused by moving the tube up and down. The focusing is done by adjusting both the coarse and fine knobs. Analyser: It is the second polariser called analyser mounted within the microscope tube. It can be pushed in and out so that it can be in the light path (inserted position) or out of the light path (analyser out position or without analyser). If the analyser is out such that it is not in the light path then the polarised light is transmitted through the ocular lens. If the analyser is in, then the plane polarised light coming from the lower polariser is blocked and no light is transmitted though the ocular lens above. We have discussed this phenomenon in the earlier section. We have discussed earlier that the rays leaving the mineral plate are broken into two vibrations. One ray is parallel to the long diagonal, which is reflected out. And the other ray is parallel to the short diagonal of the analyser that reaches the eye piece. Ocular/eye piece: It is placed at the upper end of the microscope tube. The eye piece merely enlarges the image including any imperfection resulted from poor quality objective. The eye piece in the microscope contains cross- wires. Bertrand Lens: It is used to study minerals in convergent light or under conoscopic condition. It is inserted into the upper microscope tube. Condensing Lens: It is also called condenser or convergent lens. It is a small hemispherical lens attached to a bar so that it can be inserted. This lens is used when Bertrand lens is inserted. Both condensing lens and Bertrand lens are used in case of conoscopic illumination. The polarising microscope may be assembled either for orthoscopic or conoscopic illumination. The orthoscopic arrangement provides the eye with a realistic virtual image with a flat field, showing mineral thin section on the microscopic stage. If the observation is made under an orthoscopic condition, either with the polariser alone (in case of plane polarised light) or with the analyser and polariser both (in case of cross nicol) are used. If the observations are made in conoscopic arrangement, Bertrand lens and the condenser both are used. With the help of polarising microscope, it is possible to study and identify the minerals and rocks in detail. We hope that