Polarization Imaging: Principles and Integrated Polarimeters Andreas G

Total Page:16

File Type:pdf, Size:1020Kb

Polarization Imaging: Principles and Integrated Polarimeters Andreas G 566 IEEE SENSORS JOURNAL, VOL. 2, NO. 6, DECEMBER 2002 Polarization Imaging: Principles and Integrated Polarimeters Andreas G. Andreou and Zaven Kevork Kalayjian Abstract—Polarization is a general descriptor of light and con- of Huygens, Young, Fresnel, and Maxwell to lead us to the tains information about reflecting objects that traditional inten- understanding of the polarization of light that we have today; sity-based sensors ignore. Difficult computer vision tasks such as and, its study has lead to a deeper understanding of light and image segmentation and object orientation are made tractable with polarization vision techniques. Specularities, occluding contours, light-matter interaction. and material properties can be readily extracted if the Stokes po- Polarization is used to classify chemical isomers [22], to an- larization parameters are available. Astrophysicists employ polar- alyze solar and atmospheric phenomena [23], [24], to investi- ization information to measure the spatial distribution of magnetic gate stress and strain through photoelasticity, to classify mate- fields on the surface of the sun. In the medical field, analysis of the rials and recognize objects [25]–[30], and to analyze reflections polarization allows the diagnose of disease in the eyes. The retinae of most insect and certain vertebrate species are sensitive to polar- [31], [32]. Polarimetric devices and techniques have been in- ization in their environment, but humans are blind to this prop- vented to filter, separate, quantify, and modulate polarized light. erty of light. Biologists use polarimeters to investigate behaviors of Polaroid filters are used in magneto-optic drives, liquid crystal animals—vis-à-vis polarization—in their natural habitats. In this displays, and 3-D movie glasses. Polarization is used to track paper, we first present the basics of polarization sensing and then eye movements [33], and polarization microscopes help biolo- discuss integrated polarization imaging sensors developed in our laboratory. gists see things that would otherwise escape human perception [34]. Polarization measuring instruments today are either quasi- I. INTRODUCTION static and/or do not involve an imaging device (i.e. an array of ISUALIZING the polarization of light is beyond the ca- photosensitive sites with sensitivity to the state of polarization). V pabilities of the human eye, but it is an everyday behavior The rotating polarization analyzer type polarization imaging de- for many other creatures of the Earth. Bees use the polariza- vice, for example a liquid crystal polarization camera [35], [36] tion of scattered sunlight as a compass [1], [2]. Waterstriders, can give dynamic information in the millisecond time scale. backswimmers, and dragonflies use the polarization of reflected However, in the time between measurements the brightness can light to detect water and also under-water predators [3]–[7]. change, even if slightly, making it difficulty if not impossible to Both invertebrates (e.g. mantis shrimp [8], octopi [9], crayfish disentangle brightness variations from polarization information. [10], beetles [11], flies [12]) and vertebrates (e.g. rainbow trout For real-time, portable polarization imaging, an additional [13], [14], salmon [15], sunfish [16], [17], salamanders [18], and factor is the complexity of the data processing equipment and lizards [19]) are able to sense and use polarization in their envi- their size. We have fabricated and tested a series of experi- ronments. One species of polarization sensitive cephalopod, the mental prototypes for integrated CMOS polarization imagers cuttlefish, can modulate the reflectivity of its skin, thereby ma- [37]–[40]. In this paper we present a tutorial introduction to po- nipulating the reflected pattern of polarization for camouflage or larization phenomena and discuss a number of integrated sen- during copulation—like a polarization communication channel sory devices that have been engineered for polarization imaging [20]. As a biological visual modality, polarization is prevalent [41]. among animals. Although unable to naturally sense polarization, we humans II. POLARIZATION OF LIGHT:THE BASICS have still been able to measure and analyze polarization in our environment. In the history of science, the first recorded obser- Let us consider an electromagnetic wave propagating along vation of a polarization related phenomenon was made by the the z-axis, with the e-vectors confined to oscillate along only Danish professor Erasmus Bartolinus in 1669: the double-re- one perpendicular axis, either or . fraction of light through an Iceland spar [21]. It took the likes (1) Manuscript received September 11, 2002; revised December 27, 2002. This work was supported by DARPA/ONR MURI N00014-95-1-0409 with Boston where and are the magnitude of the and components University on Automated Sensing and Vision Systems and by National Science Foundation grant EIA-0130812. The associate editor coordinating the review of of the electric field, and is the phase difference between these this paper and approving it for publication was Prof. R. W. Newcomb. two components. The appearance of in the above equa- A. G. Andreou is with the Department of Electrical and Computer Engi- tion allows for the most general case where orthogonal compo- neering, Johns Hopkins University, Baltimore MD 21218 USA. Z. K. Kalayjian is with Epigy Labs, Yerevan, Armenia. nents have different phase that may depend on spatial or tem- Digital Object Identifier 10.1109/JSEN.2003.807946 poral coordinates. Substitute for in each of the 1530-437X/02$17.00 © 2002 IEEE ANDREOU AND KALAYJIAN: POLARIZATION IMAGING: PRINCIPLES AND INTEGRATED POLARIMETERS 567 of the orthogonal components and their phase difference, the Stokes vector becomes (5) In order to fully describe the state of polarization of light, three (a) (b) (c) quantities must be known: , , and the phase difference Fig. 1. Polarization depends on the mode of oscillation of the electric field. (a) Circularly polarized, (b) linearly polarized, and (c) unpolarized. III. HOW DOES LIGHT BECOME POLARIZED? components in the equation and temporarily drop the explicit The origins of polarized radiation can be separated into two expression of the spatial and temporal dependence of in (1). categories: 1) radiation that is polarized upon emission from a polarized (2) light source; (3) 2) radiation that is originally unpolarized, but upon which the quality of polarization is subsequently imparted. By tracing the location of the tip of the electric field vector as It is interesting to note that the vast majority of polarized elec- the wave moves through space, we see that the resulting loci tromagnetic sources from category #1 are human made: lasers, ˇ describe a helix. synchrotron radiation, Cerenkov radiation, radio and television The ellipticity and orientation of the ellipse depend on the pa- broadcasts, and the Zeeman effect, to name some. Collision po- rameters . When the electric field oscillates in this larization in solar flares is one of the rare cases of a naturally mode, it is said to be elliptically polarized. In fact, if occurring polarized light source [24]. These phenomena share a , light is always elliptically polarized. The special case common trait—asymmetry or anisotropy (from a dipole, mag- where , and makes the ellipse into a netic field vector, or velocity vector) that results in a preferred circle, and the radiation is said to be circularly polarized. direction of oscillation of emitted radiation [43]. Another special case happens when and the Polarized radiation from category #2 above also depends on ellipse degenerates into a line. This is called linear polarization. an asymmetry. Unpolarized radiation, as was mentioned, ex- Depending on how the energy of the electromagnetic radiation is hibits no long-term preference for a particular oscillatory mode. distributed between the components , the electric field Consequently, in order for it to become polarized, some prefer- can be linearly polarized parallel to or . There is yet another ence has to be applied, via asymmetric work, to the unpolarized possible generally categorizable oscillatory mode that occurs radiation. This can be achieved through scattering, reflection, when and are not deterministically related. In other words, birefringence, or dichroism; that latter two will be described in there is no consistent temporal relationship between the phases more detail below as they relate to the integrated polarization of the orthogonal electric fields. In this case, the e-vector takes a sensors discussed in this paper. random path as the wave travels through space. This radiation is A. Birefringence called natural or unpolarized. Fig. 1 shows the “head-on” view for circularly, linearly, and unpolarized light. In an isotropic medium, the electric field and the displace- All the possible states of polarization can be represented in ment vector are related by the scalar , the permittivity of the one vector known as the Stokes vector [42], named after George material. However, in an anisotropic medium, permittivity is a Gabriel Stokes, who introduced it in 1852. The Stokes vector tensor quantity is a shorthand notation that describes the polarization of light through four components (6) The result of this tensor relationship in anisotropic crystals is (4) that and are not always parallel, which means that the di- rection of energy flow, or the ray direction, is not parallel to the wavefront normal, or the wave-normal
Recommended publications
  • James Clerk Maxwell
    James Clerk Maxwell JAMES CLERK MAXWELL Perspectives on his Life and Work Edited by raymond flood mark mccartney and andrew whitaker 3 3 Great Clarendon Street, Oxford, OX2 6DP, United Kingdom Oxford University Press is a department of the University of Oxford. It furthers the University’s objective of excellence in research, scholarship, and education by publishing worldwide. Oxford is a registered trade mark of Oxford University Press in the UK and in certain other countries c Oxford University Press 2014 The moral rights of the authors have been asserted First Edition published in 2014 Impression: 1 All rights reserved. No part of this publication may be reproduced, stored in a retrieval system, or transmitted, in any form or by any means, without the prior permission in writing of Oxford University Press, or as expressly permitted by law, by licence or under terms agreed with the appropriate reprographics rights organization. Enquiries concerning reproduction outside the scope of the above should be sent to the Rights Department, Oxford University Press, at the address above You must not circulate this work in any other form and you must impose this same condition on any acquirer Published in the United States of America by Oxford University Press 198 Madison Avenue, New York, NY 10016, United States of America British Library Cataloguing in Publication Data Data available Library of Congress Control Number: 2013942195 ISBN 978–0–19–966437–5 Printed and bound by CPI Group (UK) Ltd, Croydon, CR0 4YY Links to third party websites are provided by Oxford in good faith and for information only.
    [Show full text]
  • Mixing of Quantum States: a New Route to Creating Optical Activity Anvar S
    www.nature.com/scientificreports OPEN Mixing of quantum states: A new route to creating optical activity Anvar S. Baimuratov1, Nikita V. Tepliakov1, Yurii K. Gun’ko1,2, Alexander V. Baranov1, Anatoly V. Fedorov1 & Ivan D. Rukhlenko1,3 Received: 7 June 2016 The ability to induce optical activity in nanoparticles and dynamically control its strength is of great Accepted: 18 August 2016 practical importance due to potential applications in various areas, including biochemistry, toxicology, Published: xx xx xxxx and pharmaceutical science. Here we propose a new method of creating optical activity in originally achiral quantum nanostructures based on the mixing of their energy states of different parities. The mixing can be achieved by selective excitation of specific states orvia perturbing all the states in a controllable fashion. We analyze the general features of the so produced optical activity and elucidate the conditions required to realize the total dissymmetry of optical response. The proposed approach is applicable to a broad variety of real systems that can be used to advance chiroptical devices and methods. Chirality is known to occur at many levels of life organization and plays a key role in many chemical and biolog- ical processes in nature1. The fact that most of organic molecules are chiral starts more and more affecting the progress of contemporary medicine and pharmaceutical industry2. As a consequence, a great deal of research efforts has been recently focused on the optical activity of inherently chiral organic molecules3, 4. It can be strong in the ultraviolet range, in which case it is difficult to study and use in practice.
    [Show full text]
  • Applied Spectroscopy Spectroscopic Nomenclature
    Applied Spectroscopy Spectroscopic Nomenclature Absorbance, A Negative logarithm to the base 10 of the transmittance: A = –log10(T). (Not used: absorbancy, extinction, or optical density). (See Note 3). Absorptance, α Ratio of the radiant power absorbed by the sample to the incident radiant power; approximately equal to (1 – T). (See Notes 2 and 3). Absorption The absorption of electromagnetic radiation when light is transmitted through a medium; hence ‘‘absorption spectrum’’ or ‘‘absorption band’’. (Not used: ‘‘absorbance mode’’ or ‘‘absorbance band’’ or ‘‘absorbance spectrum’’ unless the ordinate axis of the spectrum is Absorbance.) (See Note 3). Absorption index, k See imaginary refractive index. Absorptivity, α Internal absorbance divided by the product of sample path length, ℓ , and mass concentration, ρ , of the absorbing material. A / α = i ρℓ SI unit: m2 kg–1. Common unit: cm2 g–1; L g–1 cm–1. (Not used: absorbancy index, extinction coefficient, or specific extinction.) Attenuated total reflection, ATR A sampling technique in which the evanescent wave of a beam that has been internally reflected from the internal surface of a material of high refractive index at an angle greater than the critical angle is absorbed by a sample that is held very close to the surface. (See Note 3.) Attenuation The loss of electromagnetic radiation caused by both absorption and scattering. Beer–Lambert law Absorptivity of a substance is constant with respect to changes in path length and concentration of the absorber. Often called Beer’s law when only changes in concentration are of interest. Brewster’s angle, θB The angle of incidence at which the reflection of p-polarized radiation is zero.
    [Show full text]
  • Polarization and Dichroism
    Polarization and dichroism Amélie Juhin Sorbonne Université-CNRS (Paris) [email protected] 1 « Dichroism » (« two colors ») describes the dependence of the absorption measured with two orthogonal polarization states of the incoming light: Circular left Circular right k k ε ε Linear horizontal Linear vertical k k ε ε 2 By extension, « dichroism » also includes similar dependence phenomena, such as: • Low symmetry crystals show a trichroic dependence with linear light • Magneto-chiral dichroism (MχD) is measured with unpolarized light • Magnetic Linear Dichroism (MLD) is measured by changing the direction of magnetic ield and keeping the linear polarization ixed … Dichroism describes an angular and /or polarization behaviour of the absorption 3 Linear dichroism (LD) : difference measured with linearly polarized light Circular dichroism (CD) : difference measured with left / right circularly polarized light. Natural dichroism (ND) : time-reversal symmetry is conserved Non-Reciprocal (NR): time-reversal symmetry is not conserved Magnetic dichroism (MD) : measured in (ferro, ferri or antiferro) magnetic materials Dichroism Time reversal Parity symmetry symmetry Natural Linear (NLD) + + Magne6c Linear (MLD) + + Non Reciprocal Linear (NRLD) - - Natural Circular (NCD) + - Magne6c Circular (MCD) - + Magneto-op6cal (MχD) - - 4 The measurement of dichroism is often challenging… … but provides access to properties that cannot be measured in another way isotropic spectra polarized spectra 9 Al K edge Al K edge [6] beryl Al 6 6 corundum -Al O α
    [Show full text]
  • Gem-Quality Tourmaline from LCT Pegmatite in Adamello Massif, Central Southern Alps, Italy: an Investigation of Its Mineralogy, Crystallography and 3D Inclusions
    minerals Article Gem-Quality Tourmaline from LCT Pegmatite in Adamello Massif, Central Southern Alps, Italy: An Investigation of Its Mineralogy, Crystallography and 3D Inclusions Valeria Diella 1,* , Federico Pezzotta 2, Rosangela Bocchio 3, Nicoletta Marinoni 1,3, Fernando Cámara 3 , Antonio Langone 4 , Ilaria Adamo 5 and Gabriele Lanzafame 6 1 National Research Council, Institute for Dynamics of Environmental Processes (IDPA), Section of Milan, 20133 Milan, Italy; [email protected] 2 Natural History Museum, 20121 Milan, Italy; [email protected] 3 Department of Earth Sciences “Ardito Desio”, University of Milan, 20133 Milan, Italy; [email protected] (R.B.); [email protected] (F.C.) 4 National Research Council, Institute of Geosciences and Earth Resources (IGG), Section of Pavia, 27100 Pavia, Italy; [email protected] 5 Italian Gemmological Institute (IGI), 20123 Milan, Italy; [email protected] 6 Elettra-Sincrotrone Trieste S.C.p.A., Basovizza, 34149 Trieste, Italy; [email protected] * Correspondence: [email protected]; Tel.: +39-02-50315621 Received: 12 November 2018; Accepted: 7 December 2018; Published: 13 December 2018 Abstract: In the early 2000s, an exceptional discovery of gem-quality multi-coloured tourmalines, hosted in Litium-Cesium-Tantalum (LCT) pegmatites, was made in the Adamello Massif, Italy. Gem-quality tourmalines had never been found before in the Alps, and this new pegmatitic deposit is of particular interest and worthy of a detailed characterization. We studied a suite of faceted samples by classical gemmological methods, and fragments were studied with Synchrotron X-ray computed micro-tomography, which evidenced the occurrence of inclusions, cracks and voids.
    [Show full text]
  • Polarization Phenomena of Certain Fluorites
    142 TIIE AMERICAN MINERALOGIST Pleochroic,e :colorless, ,:pale blue-green, It thus differs from the average vesuvianite only in slightly higher birefringence. The writer wishes to acknowledge his indebtedness to Col. Roebling, Mr. Gage, and Ward's Natural ScienceEstablishment for the privilege of examining specimensand to Dr. Larsen for the opticaldeterminations. POLARIZATION PHENOMENA OF CERTAIN FLUORITES A. L. PansoNs, Uniaersity oJ Toronto While determining the refractive index of fluorite by the method of minimum deviation the writer introduced a nicol prism before the telescopeof the goniometer to determine the plane of polarization of the refracted ray. On rotating the nicol no difference in the intensity of light could be observed,but on examining the reflected ray nearly complete polarization was obtained so that it was thought that the prism was not cut at the angle that should give polarization if Brewster's law applies to isometric crystals. A new prism was then cut from a crystal of fluorite from Madoc, Ontario, so that the angle between the reflected ray and the refracted ray should be ninety degrees. With this new prism the same phenomena were observed. A second nicol was now intro- duced between the collimator and the crystal so that the signal was extinguished. On rotating the two nicols simultaneously extinction was obtained throughout a complete rotation so that it would appear as though we may accept without question the state- ment that a ray of light passing through fluorite vibrates with equal facility in all directions at right angles to the direction of propagation and is not polarized.
    [Show full text]
  • Investigation of Dichroism by Spectrophotometric Methods
    Application Note Glass, Ceramics and Optics Investigation of Dichroism by Spectrophotometric Methods Authors Introduction N.S. Kozlova, E.V. Zabelina, Pleochroism (from ancient greek πλέον «more» + χρόμα «color») is an optical I.S. Didenko, A.P. Kozlova, phenomenon when a transparent crystal will have different colors if it is viewed from Zh.A. Goreeva, T different angles (1). Sometimes the color change is limited to shade changes such NUST “MISiS”, Russia as from pale pink to dark pink (2). Crystals are divided into optically isotropic (cubic crystal system), optically anisotropic uniaxial (hexagonal, trigonal, tetragonal crystal systems) and optically anisotropic biaxial (orthorhombic, monoclinic, triclinic crystal systems). The greatest change is limited to three colors. It may be observed in biaxial crystals and is called trichroic. A two color change may be observed in uniaxial crystals and called dichroic. Pleochroic is often the term used to cover both (2). Pleochroism is caused by optical anisotropy of the crystals Dichroism can be observed in non-polarized light but in (1-3). The absorption of light in the optically anisotropic polarized light it may be more pronounced if the plane of crystals depends on the frequency of the light wave and its polarization of incident light matches plane of polarization of polarization (direction of the electric vector in it) (3, 4). light that propagates in the crystal—ordinary or extraordinary Generally, any ray of light in the optical anisotropic crystal is wave. divided into two rays with perpendicular polarizations and The difference in absorbance of ray lights may be minor, but different velocities (v1, v2) which are inversely proportional to it may be significant and should be considered both when the refractive indices (n1, n2) (4).
    [Show full text]
  • Investigation of Magnetic Circular Dichroism Spectra of Semiconductor Quantum Rods and Quantum Dot-In-Rods
    nanomaterials Article Investigation of Magnetic Circular Dichroism Spectra of Semiconductor Quantum Rods and Quantum Dot-in-Rods Farrukh Safin 1,* , Vladimir Maslov 1, Yulia Gromova 2, Ivan Korsakov 1, Ekaterina Kolesova 1, Aliaksei Dubavik 1, Sergei Cherevkov 1 and Yurii K. Gun’ko 2 1 School of Photonics, ITMO University, 197101 St. Petersburg, Russia; [email protected] (V.M.); [email protected] (I.K.); [email protected] (E.K.); [email protected] (A.D.); [email protected] (S.C.) 2 School of Chemistry, Trinity College, Dublin 2 Dublin, Ireland; [email protected] (Y.G.); [email protected] (Y.K.G.) * Correspondence: farruhsafi[email protected] Received: 20 April 2020; Accepted: 12 May 2020; Published: 30 May 2020 Abstract: Anisotropic quantum nanostructures have attracted a lot of attention due to their unique properties and a range of potential applications. Magnetic circular dichroism (MCD) spectra of semiconductor CdSe/ZnS Quantum Rods and CdSe/CdS Dot-in-Rods have been studied. Positions of four electronic transitions were determined by data fitting. MCD spectra were analyzed in the A and B terms, which characterize the splitting and mixing of states. Effective values of A and B terms were determined for each transition. A relatively high value of the B term is noted, which is most likely associated with the anisotropy of quantum rods. Keywords: magnetic circular dichroism; quantum nanocrystals; quantum rods; Dot-in-Rods 1. Introduction Anisotropic colloidal semiconductor nanocrystals of various shapes have attracted a lot of attention over recent years due to their unique properties and potential applications.
    [Show full text]
  • GEOL 221 Mineralogy and Mineral Optics Course Syllabus
    SAN DIEGO STATE UNIVERSITY GEOL 221 Mineralogy and Mineral Optics Course Syllabus Instructor: Professor David L. Kimbrough Email: [email protected], Phone: 594-1385 Lecture: MW 1000-1050 CSL 422, Lab: W 1400-1640, Lab study: M 1400-1640 CSL 425 Office: GMCS-229A; Office hours: MW 1100-1200; T 1115-1215; by appointment TA: Mark Nahabidian GMCS-133 TBA Course Prerequisite: Chem 200 or concurrent registration. Credit or concurrent registration in OCEAN 100 or GEOL 100 and 101 or GEOL 104 and 101; Geol 200; Required Texts: Introduction to Mineralogy, William D. Nesse, Oxford University Press. Minerals in Thin Section, Perkins & Henke, Prentice Hall Recommended Text: Dictionary of Geological Terms, AGI Bates & Jackson eds. or similar, The Complete Guide to Rocks & Minerals by John Farndon or similar Other required materials: Hand lens; calculator Classroom management: Attendance is crucial. Please let me know if you’re going to be absent for any reason. Be on time for class, don’t participate in excessive side-chatter or cause disruptions during class. Always respond to the instructor, the Teaching Assistant, and your fellow students in a respectful and civil manner. Cheating or plagiarism is not tolerated. It’s easy to spot and constitutes serious academic misconduct. Helpful hints to make Mineralogy easier and more fun! Attend class: Studies show that the most valuable time commitment by students in a course is the time actually spent in the classroom. Class time is the most important determinant of student success and yields the greatest improvement in student learning outcomes. Information is covered in class that is not in the textbook, and parts of the book are hard to understand.
    [Show full text]
  • Copper-Bearing (Paraíba-Type) Tourmaline from Mozambique
    COPPER-BEARING (PARAÍBA-TYPE) TOURMALINE FROM MOZAMBIQUE Brendan M. Laurs, J. C. (Hanco) Zwaan, Christopher M. Breeding, William B. (Skip) Simmons, Donna Beaton, Kenneth F. Rijsdijk, Riccardo Befi, and Alexander U. Falster Copper-bearing tourmaline from Mozambique was first recovered in 2001, but its Cu content was not recognized until 2003, and it was not widely sold with its Mozambique origin disclosed until 2005. It has been mined from alluvial deposits in an approximately 3 km2 area near Mavuco in the eastern portion of the Alto Ligonha pegmatite district. Most of the production has come from arti- sanal mining, with hand tools used to remove up to 5 m of overburden to reach the tourmaline- bearing layer. The stones exhibit a wide range of colors, typically pink to purple, violet to blue, and blue to green or yellowish green. Heat treatment of all but the green to yellowish green stones typi- cally produces Paraíba-like blue-to-green hues by reducing absorption at ~520 nm caused by the presence of Mn3+. The gemological properties are typical for Cu-bearing tourmaline (including material from Brazil and Nigeria); the most common inclusions consist of partially healed fractures and elongate hollow tubes. With the exception of some green to yellow-green stones, the tourma- lines examined have relatively low Cu contents and very low amounts of Fe and Ti. Mechanized mining is expected to increase production from this region in the near future. opper-bearing tourmaline, in bright blue- deposit of Cu-bearing tourmaline, and the “neon” to-green hues, is one of the most sought- blue and green shown by the finest stones closely after colored stones in the gem market.
    [Show full text]
  • Limits on Fluorescence Detected Circular Dichroism of Single Helicene Molecules
    6213 2009, 113, 6213–6216 Published on Web 05/13/2009 Limits on Fluorescence Detected Circular Dichroism of Single Helicene Molecules Yiqiao Tang,† Timothy A. Cook,‡,§ and Adam E. Cohen*,†,‡ Department of Physics, HarVard UniVersity, Cambridge, Massachusetts 02138, and Department of Chemistry and Chemical Biology, HarVard UniVersity, Cambridge, Massachusetts 02138 ReceiVed: April 19, 2009; ReVised Manuscript ReceiVed: May 6, 2009 Fluorescent imaging of single helicene molecules is applied to study the optical activity of chiral fluorophores. In contrast to the previous report by Hassey et al. (Science 2006, 314, 1437), the dissymmetry factors of single chiral fluorophores are found not to differ significantly from the bulk value of |g| < 10-4 at 457 nm. Linear dichroism and birefringence of the dichroic mirror inside the fluorescence microscope change the polarization state of the incoming laser beam significantly; i.e., circular polarized light sent into the microscope becomes highly elliptically polarized after reflection from the dichroic mirror. Compensation for this effect should be made to avoid artifacts brought by linear dichroism in single immobilized molecules. Single-molecule measurements have been used to probe the H )-µ · E - θ:∇E - m · B states and dynamics of a huge variety of biophysical and condensed matter systems,2-4 and often yield information that where µ is the electric dipole operator, θ is the electric is missed by bulk, ensemble-averaged techniques. In particular, quadrupole operator, and m is the magnetic dipole operator; E measurements at ambient and low temperatures provide detailed is the electric field, and B is the magnetic field. The rate of information about the interactions between single fluorescent excitation from an initial state i to a final state f involves the 2 molecules and their local environment.
    [Show full text]
  • B.Sc.( Srmester-3) Subject: Physics Course: US03CPHY01 Title: Optics
    B.Sc.( Srmester-3) Subject: Physics Course: US03CPHY01 Title: Optics UNIT – III Polarization Introduction:- • Interference and diffraction phenomena proved that light is a wave motion. These phenomena are used to find wavelength of light. However, they do not give any indication regarding the character of waves. • Maxwell developed electromagnetic theory and suggested that light-waves are electromagnetic waves. Electromagnetic waves are transverse waves, so it is obvious that light waves are also transverse waves. • Longitudinal waves are waves in which particles of medium oscillate along the direction of propagation of wave (e.g. sound wave). • Transverse waves are waves in which particles of medium oscillate perpendicular to the direction of propagation of wave. (e.g. Electromagnetic waves.) • Polarization is possible in transverse wave. • Unpolarized Light is the light is which the planes of vibration are symmetrically distributed about the propagation direction of the wave. • Plane Polarized light is a wave in which the electric vector is everywhere confined to a single plane. • A linearly polarized light wave is a wave in which the electric vector oscillates in a given constant orientation. Production of Linearly Polarized Light: Linearly polarized light may be produced from unpolarized light using following optical phenomena. (i) Reflection (ii) Refraction (iii) Scattering (iv) Selective absorption (v) Double reflection. Polarized light has many important applications in industry and engineering. One of the most important applications is in liquid crystal displays (LCDs) which are widely used in wristwatches, calculators, T.V. Screens etc. An understanding of polarization is essential for understanding the propagation of electromagnetic waves guided through wave-guides and optical fibers.
    [Show full text]