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Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry
Western Michigan University ScholarWorks at WMU Master's Theses Graduate College 12-2012 Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry Kari Blain Follow this and additional works at: https://scholarworks.wmich.edu/masters_theses Part of the Chemistry Commons Recommended Citation Blain, Kari, "Direct Analysis of Hops by Leaf Spray and Paper Spray Mass Spectrometry" (2012). Master's Theses. 100. https://scholarworks.wmich.edu/masters_theses/100 This Masters Thesis-Open Access is brought to you for free and open access by the Graduate College at ScholarWorks at WMU. It has been accepted for inclusion in Master's Theses by an authorized administrator of ScholarWorks at WMU. For more information, please contact [email protected]. DIRECT ANALYSIS OF HOPS BY LEAF SPRAY AND PAPER SPRAY MASS SPECTROMETRY Kari Blain, M.S. Western Michigan University, 2012 The objective of this research is to develop a new and innovative method of hops analysis, which is much faster than standard testing methods, as well as reduce the amount of consumables and solvent used. A detailed discussion on the development of an ambient ionization mass spectrometry method called paper spray (PS-MS) and leaf spray (LS-MS) mass spectrometry will be presented. This research investigates the use of PS-MS and LS-MS techniques to determine the α- and β- acids present in hops. PS-MS and LS-MS provide a fast way to analyze hops samples by delivering data as rapidly as a UV-Vis measurement while providing information similar to lengthy liquid chromatographic separations. The preliminary results shown here indicate that PS-MS could be used to determine cohumulone and α/β ratios. -
A Phased, Diploid Assembly of the Cascade Hop (Humulus Lupulus) Genome Reveals Patterns of Selection and Haplotype Variation
bioRxiv preprint doi: https://doi.org/10.1101/786145; this version posted September 28, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. A phased, diploid assembly of the Cascade hop (Humulus lupulus) genome reveals patterns of selection and haplotype variation Lillian K. Padgitt-Cobb1, Sarah B. Kingan2, Jackson Wells3, Justin Elser6, Brent Kronmiller3, Daniel Moore4, Gregory Concepcion2, Paul Peluso2, David Rank2, Pankaj Jaiswal6, John Henning4*, David A. Hendrix1,5* 1 Department of Biochemistry and Biophysics, Oregon State University 2 Pacific Biosciences of California, 3 CGRB, Oregon State University 4 USDA ARS 5 School of Electrical Engineering and Computer Science, Oregon State University 6 Department of Botany and Plant Pathology, Oregon State University * to whom correspondence should be addressed: [email protected], [email protected] bioRxiv preprint doi: https://doi.org/10.1101/786145; this version posted September 28, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY 4.0 International license. Abstract Hop (Humulus lupulus L. var Lupulus) is a diploid, dioecious plant with a history of cultivation spanning more than one thousand years. Hop cones are valued for their use in brewing, and around the world, hop has been used in traditional medicine to treat a variety of ailments. -
Hops – a Guide for New Growers 2017
Hops – a guide for new growers 2017 growers new – a guide for Hops Hops a guide for new growers FIRST EDITION 2017 first edition 2017 Author: Kevin Dodds www.dpi.nsw.gov.au Hops a guide for new growers Kevin Dodds Development Officer – Temperate Fruits NSW Department of Primary industries ©NSW Department of Primary Industries 2017 Published by NSW Department of Primary Industries, a part of NSW Department of Industry, Skills and Regional Development You may copy, distribute, display, download and otherwise freely deal with this publication for any purpose, provided that you attribute NSW Department of Industry, Skills and Regional Development as the owner. However, you must obtain permission if you wish to charge others for access to the publication (other than at cost); include the publication advertising or a product for sale; modify the publication; or republish the publication on a website. You may freely link to the publication on a departmental website. First published March 2017 ISBN print: 978‑1‑76058‑007‑0 web: 978‑1‑76058‑008‑7 Always read the label Users of agricultural chemical products must always read the Job number 14293 label and any permit before using the product and strictly comply with the directions on the label and the conditions of Author any permit. Users are not absolved from any compliance with Kevin Dodds, Development Officer Temperate Fruits the directions on the label or the conditions of the permit NSW Department of Primary Industries by reason of any statement made or omitted to be made in 64 Fitzroy Street TUMUT NSW 2720 this publication. -
International Hop Growers Convention
INTERNATIONAL HOP GROWERS’ CONVENTION ECONOMIC COMMISSION - SUMMARY REPORTS VIDEO-CALL NOVEMBER, 2020 International Hop Growers’ Convention IHGC - Economic Commission Summary Reports Video-Call - November 9, 2020 Hop Acreage 2019 Hop Production 2019 Alpha Hop Acreage 2020 Hop Production 2020 Alpha Country (Hectares, Ha) (in MT = 1.000 kg) acid (Hectares, Ha) (in MT = 1.000 kg) acid Prod. estimations estimations Prod. Aroma Alpha Hop area' New Total Aroma Alpha Total MT Aroma Alpha Hop area' New Total Aroma Alpha Total MT Argentina 118 56 174 0 174 188 78 266 23 127 59 186 11 186 230 101 331 28 Australia° 476 176 652 48 700 1 385 260 1 645 224 548 152 700 43 743 1 478 236 1 714 255 Austria 194 58 253 3 253 368 124 492 37 197 58 255 5 255 360 117 487 39 Belgium 124 58 182 0 182 172 124 296 24 124 58 182 0 182 154 122 276 21 Canada° 219 200 419 0 419 329 300 629 63 200 150 350 0 350 329 300 525 53 China° 400 2 283 2 683 0 2 683 544 6 500 7 044 600 360 2 000 2 360 0 2 360 544 6 500 7 044 600 Czech Republic 4 706 49 4 755 248 5 003 6 991 154 7 145 285 4 635 61 4 696 270 4 966 5 830 120 5 950 265 France 424 42 466 39 505 728 94 822 25 413 48 460 40 500 672 95 767 31 Germany 10 860 8 913 19 773 644 20 417 22 584 25 888 48 472 5 260 10 973 9 154 20 127 579 20 706 21 400 25 600 47 000 5 400 Japan° 53 53 106 0 106 82 120 202 25 53 53 106 0 106 82 120 202 25 New Zealand° 598 145 743 331 743 780 239 1 019 100 598 145 743 331 743 1 000 250 1 250 115 Poland 636 1 020 1 656 106 1 762 1 293 2 476 3 765 317 650 1 111 1 761 30 1 791 1 155 2 262 3 417 354 -
Crp 305 Crop Genetics and Breeding
CRP 305 CROP GENETICS AND BREEDING NATIONAL OPEN UNIVERSITY OF NIGERIA 1 COURSE CODE: CRP 305 COURSE TITLE: CROPS GENETICS AND BREEDING COURSE DEVELOPER: COURSE WRITER: PROFESSOR LATEEF LEKAN BELLO DEPARTMENT OF PLANT BREEDING AND SEED SCIENCE UNIVERSITY OF AGRICULTURE MAKURDI BENUE STATE COURSE EDITOR: COURSE CO-ORDINATOR: 2 COURSE GUIDE CRP 305 CROP GENETICS AND BREEDING 3 TABLE OF CONTENTS Introduction What you will learn in this course Course Aims Course Objectives Course Requirements Course Materials Study Units Textbooks and References Assessment Tutor Marked Assignment Final Examination and Grading Summary 4 INTRODUCTION CRP 305 Crop Genetics and Breeding is a one semester two (2) credit units course designed for undergraduate students in 300 Level. The course consists of three major parts, with five modules and eighteen 18 units. In writing this study guide, I have given primary consideration to the students‟ background. It has been assumed that students usually enroll in the crop genetics and breeding course after they have completed courses in botany, genetics statistics and other related courses in agriculture. WHAT YOU WILL LEARN IN THIS COURSE CRP 305 – Crop genetics and breeding consists of five major components arranged in modules. The first module which is on crop genetics and breeding will introduce you to the concept of what: a. Crop breeding is, and the purpose is suppose to serve. b. Plant cell concepts, gene symbols which will help you to understand Mendel‟s experiments and Law of Inheritance. c. Differences between qualitative and quantitative traits constitute. The study in the second module: You shall learn about heritability and selection responses. -
PLANT BREEDING David Luckett and Gerald Halloran ______
CHAPTER 4 _____________________________________________________________________ PLANT BREEDING David Luckett and Gerald Halloran _____________________________________________________________________ WHAT IS PLANT BREEDING AND WHY DO IT? Plant breeding, or crop genetic improvement, is the production of new, improved crop varieties for use by farmers. The new variety may have higher yield, improved grain quality, increased disease resistance, or be less prone to lodging. Ideally, it will have a new combination of attributes which are significantly better than the varieties already available. The new variety will be a new combination of genes which the plant breeder has put together from those available in the gene pool of that species. It may contain only genes already existing in other varieties of the same crop, or it may contain genes from other distant plant relatives, or genes from unrelated organisms inserted by biotechnological means. The breeder will have employed a range of techniques to produce the new variety. The new gene combination will have been chosen after the breeder first created, and then eliminated, thousands of others of poorer performance. This chapter is concerned with describing some of the more important genetic principles that define how plant breeding occurs and the techniques breeders use. Plant breeding is time-consuming and costly. It typically takes more than ten years for a variety to proceed from the initial breeding stages through to commercial release. An established breeding program with clear aims and reasonable resources will produce a new variety regularly, every couple of years or so. Each variety will be an incremental improvement upon older varieties or may, in rarer circumstances, be a quantum improvement due to some novel gene, the use of some new technique or a response to a new pest or disease. -
QTL Mapping of Powdery Mildew Susceptibility in Hop (Humulus Lupulus L.)
QTL mapping of powdery mildew susceptibility in hop (Humulus lupulus L.) John A. Henning, M. Shaun Townsend, David H. Gent, Nahla Bassil, Paul Matthews, Emily Buck & Ron Beatson Euphytica International Journal of Plant Breeding ISSN 0014-2336 Volume 180 Number 3 Euphytica (2011) 180:411-420 DOI 10.1007/ s10681-011-0403-4 1 23 Your article is protected by copyright and all rights are held exclusively by Springer Science+Business Media B.V.. This e-offprint is for personal use only and shall not be self- archived in electronic repositories. If you wish to self-archive your work, please use the accepted author’s version for posting to your own website or your institution’s repository. You may further deposit the accepted author’s version on a funder’s repository at a funder’s request, provided it is not made publicly available until 12 months after publication. 1 23 Author's personal copy Euphytica (2011) 180:411–420 DOI 10.1007/s10681-011-0403-4 QTL mapping of powdery mildew susceptibility in hop (Humulus lupulus L.) John A. Henning • M. Shaun Townsend • David H. Gent • Nahla Bassil • Paul Matthews • Emily Buck • Ron Beatson Received: 13 October 2010 / Accepted: 28 February 2011 / Published online: 15 March 2011 Ó Springer Science+Business Media B.V. (outside the USA) 2011 Abstract Hop powdery mildew [Podosphaera mac- was extracted, purified and analyzed via three ularis (Wallr.) U. Braun & S. Takam.] is best different marker systems. Analysis of the resulting controlled via the production of resistant varieties. markers was based upon the ‘‘two-way pseudo- Recent evidence supports selection against plant testcross’’ procedure. -
The Feasibility of Using Diploid by Tetraploid Crosses to Obtain Triploid Hops
AN ABSTRACT OF THE THESIS OF Jeanine Streeter De Noma for the degree of Master of Science in Crop Science presented on November 4, 1994. Title: The Feasibility of Using Diploid by Tetraploid Crosses to Obtain Triploid Hops (Humulus lupulus L.) Redacted for Privacy Abstract approved: r. Alfred Haunold The hop (Humulus lupulus L.) is a dioecious, diploid (2n=2x=20) species. Triploid hops (2n=3x=30) are easily obtained from tetraploid by diploid crosses and are preferred because of their natural seedlessness and increased vigor. The female inflorescence is used for flavoring beer. Because brewers require product consistency, the brewing characteristics of a new aroma-type hop must closely match the variety it is intended to replace while showing improved adaptation and agronomic traits. Since triploid offspring should more closely resemble the tetraploid parent from which they receive two-thirds of their genetic material, breeders have traditionally selected known varieties to be the tetraploid parent with the expectation this will increase the likelihood of obtaining progeny with similar brewing chemistry. Tetraploid female parents are obtained through colchicine treatment of desirable diploid varieties. Naturally occurring tetraploid seedlings, sometimes found among the offspring of tetraploid by diploid crosses, if suitable for breeding, could eliminate the tedious colchicine treatment process now used to obtain tetraploids. Two such tetraploid males were obtained from an Oregon cross in 1983. Since these two tetraploid males had attractive quality traits similar to other males in the USDA germplasm collection with a proven history of producing aroma-type progeny, they were thought to be suitable for use in a polyploid breeding program for aroma hops. -
Pilsner Clone Recipes
the best of ® 15 Pilsner Clone Recipes Please note all file contents are Copyright © 2020 Battenkill Communications, Inc. All Rights Reserved. This file is for the buyer’s personal use only. It’s unlawful to share or distribute this file to others in any way including e-mailing it, posting it online, or sharing printed copies with others. BIERSTADT LAGERHAUS’ BIERSTADT LAGERHAUS’ SLOW POUR PILS CLONE SLOW POUR PILS CLONE (ALL-GRAIN) (EXTRACT ONLY) (5 gallons/19 L, all-grain) (5 gallons/19 L, extract only) OG = 1.047 FG = 1.012 OG = 1.050 FG = 1.012 IBU = 33 SRM = 3 ABV = 4.5 % IBU = 33 SRM = 5 ABV = 4.9% Ingredients Ingredients 9.0 lbs. (4.08 kg) German Pilsner malt 5.5 lbs. (2.49 kg) Pilsen dried malt extract 0.5 lb. (0.23 kg) acidulated malt 4 AAU Hallertau Mittelfrüh hops (first wort hopping) 4 AAU Hallertau Mittelfrüh hops (first wort hopping) (1 oz./30 g at 4% alpha acids) (1 oz./30 g at 4% alpha acids) 4 AAU Hallertau Mittelfrüh hops (70 min.) 4 AAU Hallertau Mittelfrüh hops (70 min.) (1 oz./30 g at 4% alpha acids) (1 oz./30 g at 4% alpha acids) Wyeast 2124 (Bohemian Lager) or White Labs WLP830 Wyeast 2124 (Bohemian Lager) or White Labs WLP830 (German Lager) or Saflager W-34/70 yeast 3 (German Lager) or Saflager W-34/70 yeast ⁄4 cup corn sugar (if priming) 3 ⁄4 cup corn sugar (if priming) Step by Step Step by Step Bring 5.0 gallons (18.9 L) of water to a boil. -
Washington Hop Commission Hop Growers of America Hop Growers Of
Washington Hop Commission Hop Growers of America Hop Growers of Washington US Hop Industry Plant Protection Committee >>> 2018 KEY EVENTS <<< BREWCON (Sydney, Australia) Annual Meeting & JUN American Hop Convention, Hop Growers of America TU Berlin 26-28 CBC (Palm Desert, CA) (Berlin, Germany) (Nashville, TN) JAN APR 30 JUN 24-26 - MAY 3 5 JUN 6 MAR 7-8 MAY Doemens Brewing JAN School Seminar Hill visits in D.C. w/ BA & HRC 18 (Munich, Germany) 5 (Washington, D.C.) Annual Meeting, Hop Growers of Washington (Yakima) IHGC Executive Commiee (Paris, France) A Look Back at 2018 In an effort to demonstrate to members what is being done on their behalf throughout the year, Washington Hop Commission staff have put together this report to communicate a com- prehensive Annual Report for work done for all of the organizations they work on behalf of: the Washington Hop Commission, Hop Growers of America, Hop Growers of Washington, and the U.S. Hop Industry Plant Protection Commiee. As many efforts benefit multiple parties, we have not sectioned this off by organization, but the report has been organized by different themes – International Markets, Technical & Regu- latory work, Staff & Office Changes, etc. 2 CERIA (Brussels, Belgium) Heriot-Wa Brewing School Seminar (Edinburgh, Scotland) OCT Eastern Europe 12 Cra Brewing Seminar NOV (Prague, Czech Republic) 6 JUL IHGC 31 NOV Executive Commiee (Nurem- OCT 7 berg, Germany) IHGC 24-27 Executive Commiee (Leon, Spain) NOV Warsaw Beer Festival 12 (Warsaw, Poland) NOV 8 NOV Weihenstephan TUM Brewing 13-15 School Seminar (Munich, Germany) BrauBeviale, Trade Show & Tasting Seminar Contents: (Nuremburg, Germany) INFRASTRUCTURE ......................................... -
My Brewery Experience
Multi-award-winning magazine of the Bristol & District and Bath & Borders branches of CAMRA, the Campaign for Real Ale No. 115 Autumn 2017 PINTS WEST My Brewery Experience Day Contents Page 22 BADRAG (rare ales group) Page 24 Bath & Borders pub news Snuffy Jack’s Ale House Page 28 Bath Winter Ales Festival Page 46 Bristol Beer Week Page 34 Bristol Pubs Group off to a flying start Page 51 CAMRA diaries & contacts Page 42 CAMRA Good Beer Guide ristol’s third micropub – Snuffy Jack’s Ale House in Fishponds – INTS WES Page 48 CAMRA ladies opened at noon on the 24th June 2017 and has instantly proven Page 49 CAMRA young(ish) members Breally popular, building up a good level of trade and many P T Page 44 Champion Beer of Britain regular customers, despite a high number of other pubs and bars The multi-award-winning magazine of the Bristol & District Page 10 My brewery experience day nearby. The building was previously Osbournes Stationers and then a branch of CAMRA, the Campaign for Real Ale, plus the Bath Page 41 Portishead short-lived firework shop. & Borders branch Page 32 Shine on Westbury-on-Trym Brought to you entirely by unpaid volunteers Page 45 Toulouse The name relates to a former head miller at the nearby Snuff Mills. Ten thousand copies of Pints West are distributed free to Co-owners Johnny Fallon (on the right in the middle photo) from Page 3 Snuffy Jack’s micropub Manchester and Dave Sibley from Northampton met in the Royal Navy hundreds of pubs in and around the cities of Bristol and Bath Page 39 Weston-super-Mare news many years ago and have now settled in Bristol. -
WELCOME to Our Region!
WELCOME T O O UR R E GI O N! H O S T N A T I O N C O U nc I L S PA N GDA H L E M E .V. 2 GERMAN-AMERICAN We would like to consider this wonderful German- American relationship as indispensable for both na- FRIENDSHIP – A STRONG tions. ALLIANCE And when Spangdahlem Air Base celebrated its 50th anniversary in 2003 German political, economical and HOST NATION COUncIL – AN INITIATIVE social representatives took advantage of this opportu- FOR US CITIZENS IN THE REGION nity and realized a long cherished desire: Foundation of the Host Nation Council. In 1953 this region gained a very important institution: With this foundation, the Host Nation Council wanted Spangdahlem Air Base! to enhance the already existing strong support for all What an enormous new experience for the people in American neighbours, underline the importance of the the Eifel! local US Forces presence and stress the significant meaning of our friendship. We are striving for the Eifel Although, first rather cautious, people in the Eifel to become your ’Home away from Home‘. And last, soon developed a certain curiosity towards these but not least, the Council's foundation is to serve as a new neighbours coming from an entirely different sign of gratitude for over 50 years of liberty, freedom part of the world. Soon their culture, their customs and democracy, guaranteed by the US leadership and and courtesies were very much appreciated and had its service members. more and more a formative influence on the life of the Eifel citizens thus presenting a mutual enrichment for The Council's chairman and head of the Board is both nations, which again resulted in a unique and Jan Niewodniczanski with Michael Billen harmonious living together.