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Frimley Health NHS Foundation Trust Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Staines-upon-Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust Issue No: 006 Issue date: 05 August 2021 Frimley Health NHS Foundation Contact: Governance / Quality Department Trust Tel: +44 (0) 1276 522194 / 1276 522541 / 1276 522175 Frimley Park Hospital, 8086 E-Mail: [email protected] Frimley Website: www.nhspathology.fph.nhs.uk Accredited to Camberley ISO 15189:2012 GU16 7UJ Testing performed by the Organisation at the locations specified below Locations covered by the organisation and their relevant activities Laboratory locations: Location details Activity Location code Address Local contact Histology RBH Cellular Pathology Department Amanda Babington Immunocytochemistry Tel: +44 (0) 118322 8201 Frozen Section for intraoperative Royal Berkshire Hospital diagnosis. E-Mail: London Road Interpretive microscopy Amanda.babington@Roy Non-Gynae Cytology Reading alberkshire.nhs.uk RG1 5AN Address Local contact Histology WPH Department of Histopathology Stephen Brown Immunocytochemistry Tel: +44 (0) 300 615 Frozen Section for intraoperative Wexham Park Hospital 3458 diagnosis. Slough E-Mail: Interpretive microscopy Non-Gynae Cytology (cell block or clot Berkshire [email protected] processing and staining) SL2 4HL Local contact Address Harvi Atwal OSNA (one-step nucleic acid HH Tel: +44 (0) 118 322 amplification) Heatherwood Hospital 7738 High St Ascot E-Mail: SL5 8AA Harvinder.Atwal@royalbe rkshire.nhs.uk Assessment Manager: DE 1 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified Site activities performed away from the locations listed above: Location details Activity Locatio n code One stop breast FNA clinic Local contact Non-Gynae cytology specimen collection A Outpatients 2 Department Amanda BabingtonTel: Royal Berkshire NHS Foundation Trust +44 (0) 118 322 8201 Hospital E-Mail: London Road Amanda.babington@Roy Reading alberkshire.nhs.uk RG1 5AN Assessment Manager: DE Page 2 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified DETAIL OF ACCREDITATION Materials/Products tested Type of test/Properties Standard specifications/ Location measured/Range of Equipment/Techniques used Code measurement HUMAN BODY TISSUE AND Histopathological examination In-house documented procedures RBH FLUIDS activities for the purposes of using manual methods or analysers clinical diagnosis as specified, in conjunction with manufacturers’ instructions: Tissue samples: Fixed, fresh The examination of tissues to Macroscopic and Microscopic RBH and frozen, including Bone exclude or identify examination Marrow trephines. morphological and cytological Non-gynae cytology cell abnormalities for the purpose Supported by documented in house blocks of clinical diagnosis methods incorporating manufacturer’s instructions where relevant: Formalin-fixed tissue samples Tissue Dissection HIS-RBH-SOP-001 Histology cut- RBH up HIS-RBH-SOP-002 Dissection of small spec HIS-RBH-SOP-003 Dissection of large spec Thermofisher Printmate HIS-WPH-SOP-0077 Medical Specimen Dissection Formalin-fixed tissue samples Decalcification RBH HIS-RBH-SOP-019 Decalcifier TDE TM 30 Formalin-fixed tissue samples Tissue Processing including Non-gynae cytology cell blocks HIS-RBH-SOP-006 RBH Tissue Processing Thermofisher Excelsior, STP 420 Sakura VIP 6AI WPH HIS-WPH-SOP-0100 Sakura VIP tissue processors. Assessment Manager: DE Page 3 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified Materials/Products tested Type of test/Properties Standard specifications/ Location measured/Range of Equipment/Techniques used Code measurement HUMAN BODY TISSUE AND Histopathological examination In-house documented procedures FLUIDS activities for the purposes of using manual methods or analysers (cont’d) clinical diagnosis cont’d) as specified, in conjunction with manufacturers’ instructions: Tissue Embedding RBH Formalin-fixed tissue samples HIS-RBH-SOP-005 Embedding including Non-gynae cytology Thermofisher Histostars cell blocks WPH HIS-WPH-SOP-0100 Sakura TEC 5 and 6 Embedding centre Formalin Fixed paraffin wax Tissue Sectioning blocks of processed tissue, including Non-gynae cytology HIS-RBH-SOP-007 RBH cell blocks Section Cutting Leica RM2235, Sakura Accu-cut SRM200 & Microm HM325 WPH MicrotomesHIS-WPH-SOP-0204 Leica RM2235 Fresh/frozen tissue Frozen sections (cryotomy) HIS-RBH-SOP-011 Cryomicrotomy RBH and Intraoperative Frozen Sections. Thermo Cryostar NX50 HIS-WPH-SOP-0300 WPH Leica CM1950 cryostats. Assessment Manager: DE Page 4 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified Materials/Products tested Type of test/Properties Standard specifications/ Location measured/Range of Equipment/Techniques used Code measurement HUMAN BODY TISSUE AND Histopathological examination In-house documented procedures FLUIDS activities for the purposes of using manual methods or analysers (cont’d) clinical diagnosis cont’d) as specified, in conjunction with manufacturers’ instructions: Formalin-fixed paraffin Demonstration of basophilic Automated Haematoxylin and RBH, embedded tissue slides and eosinophilic structures Eosin Staining Roche HE600 Coverstainer Fresh/frozen tissue HIS-RBH-SOP-026; RBH Frozen section Haematoxylin and Eosin Staining procedures: HIS-RBH-SOP-011 MYREVA Automated stainer HIS-RBH-SOP-038 HIS-WPH-SOP-0217 HIS-WPH-SOP-0400 Sakura Prisma and Sakura DRS WPH 2000 automated staining machines Demonstration of: Special Tinctorial Histochemical RBH Formalin-fixed paraffin Staining Methods embedded tissue slides (cont’d) Documented in house procedures for manual specials stains SOP H9 Acid mucins Alcian Blue Technique RBH Mucicarmine Technique Acid and Neutral mucins Alcian Blue – Periodic Acid-Schiff ± RBH Diastase Amyloid - Primary Congo Red with Potassium RBH Permanganate Amyloid – Secondary Congo Red (Highman) RBH Amyloid deposits Sirius Red Assessment Manager: DE Page 5 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified Materials/Products tested Type of test/Properties Standard specifications/ Location measured/Range of Equipment/Techniques used Code measurement Special Tinctorial Histochemical HUMAN BODY TISSUE AND Histopathological Staining Methods FLUIDS examination activities for the (cont’d) (cont’d) purposes of clinical diagnosis cont’d) Formalin-fixed paraffin Basement membranes Jones Methenamine (Hexamine) RBH embedded tissue slides Silver method. (cont’d) Bacteria Gram Stain. RBH Bile Pigments Fouchet Technique RBH Bone marrow cell Giemsa Stain. RBH differentiation Calcium Von Kossa Technique RBH Colloidal Iron Dialyzed Iron stain. RBH Elastin Van Gieson Verhoeff’s Stain. RBH Fibrin MSB Stain. RBH Fungi or Pneumocystis Grocott Methenamine (Hexamine) RBH Silver Method. Glycogen PAS ± Diastase. RBH Haemosiderin Perl’s Prussian Blue Stain. RBH Hepatitis B Antigen Orcein (Shikata) Stain. RBH Helicobacter Toluidine Blue. RBH Helicobacter – modified Giemsa RBH Frozen tissue Lipids Oil Red O RBH Mast Cells Toluidine Blue. RBH Assessment Manager: DE Page 6 of 21 Schedule of Accreditation issued by United Kingdom Accreditation Service 2 Pine Trees, Chertsey Lane, Sta ine s -u po n - Thames, TW18 3HR, UK Frimley Health NHS Foundation Trust 8086 Issue No: 006 Issue date: 05 August 2021 Accredited to ISO 15189:2012 Testing performed by the Organisation at the locations specified Materials/Products tested Type of test/Properties Standard specifications/ Location measured/Range of Equipment/Techniques used Code measurement HUMAN BODY TISSUE AND Histopathological examination Special Tinctorial Histochemical FLUIDS activities for the purposes of Staining Methods (cont’d) clinical diagnosis cont’d) (cont’d) Frozen tissue (cont’d) Melanin Masson Fontana Silver Overnight RBH Masson Fontana Heated Method Melanin Bleach Method. M Leprae Wade-Fite Method. RBH M Tuberculosis Ziehl-Neelsen RBH Formalin-fixed paraffin embedded tissue slides (cont’d) Reticulin Fibres Gordon and Sweet Stain. RBH Smooth muscle/Collagen Haematoxylin and Van Gieson RBH Spirochaetes Warthin Starry Method RBH Demonstration of: Demonstration of: WPH Special Tinctorial Histochemical Staining Methods Documented in house procedures for manual specials stains HIS-WPH-SOP-0400
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