SAN TA C RUZ BI OTEC HNOL OG Y, INC . SLC26A8 (N-15): sc-169329

BACKGROUND PRODUCT SLC26A8 (solute carrier family 26, member 8) is a 970 amino acid multi-pass Each vial contains 200 µg IgG in 1.0 ml of PBS with < 0.1% sodium azide membrane protein that contains 14 putative transmembrane regions with cyto - and 0.1% gelatin. plasmic N- and C-termini. SLC26A8 shares 26% identity with , 28% Blocking peptide available for competition studies, sc-169329 P, (100 µg identity with DRA, and interacts with RACGAP1. SLC26A8 acts as a DIDS- peptide in 0.5 ml PBS containing < 0.1% sodium azide and 0.2% BSA). sensitive anion exchanger mediating chloride, sulfate and oxalate transport. It has been suggested that SLC26A8 fulfills critical anion exchange functions in APPLICATIONS male germ line during meiosis and hence may play a role in spermatogene sis. SLC26A8 may also be involved in a new regulatory pathway linking sulfate SLC26A8 (N-15) is recommended for detection of SLC26A8 of mouse, rat and transport to RhoGTPase signaling in male germ cells. As a critical compo nent human origin by Western Blotting (starting dilution 1:200, dilution range of the sperm annulus, SLC26A8 is essential for correct sperm tail differentia - 1:100-1:1000), immunofluorescence (starting dilution 1:50, dilution range tion and motility and hence male fertility. Existing as four alternatively spliced 1:50-1:500) and solid phase ELISA (starting dilution 1:30, dilution range isoforms, the SLC26A8 is conserved in chimpanzee, canine, bovine, 1:30- 1:3000); non cross-reactive with other SLC26A family members. mouse, rat and chicken, and maps to human 6p21.31. The SLC26A8 (N-15) is also recommended for detection of SLC26A8 in additional SLC26A8 gene contains 20 exons and spans about 80 kb. species, including equine, canine, bovine and porcine.

REFERENCES Suitable for use as control antibody for SLC26A8 siRNA (h): sc-95583, SLC26A8 siRNA (m): sc-153526, SLC26A8 shRNA Plasmid (h): sc-95583-SH, 1. Toure, A., et al. 2001. Tat1, a novel specifically SLC26A8 shRNA Plasmid (m): sc-153526-SH, SLC26A8 shRNA (h) Lentiviral expressed in human male germ cells and potentially linked to rhogtpase Particles: sc-95583-V and SLC26A8 shRNA (m) Lentiviral Particles: signaling. J. Biol. Chem. 276: 20309-20315. sc-153526-V. 2. Vincourt, J.B., et al. 2002. Molecular cloning of SLC26A7, a novel member Molecular Weight of SLC26A8 isoforms 1/2/3/4: 109/97/62/86 kDa. of the SLC26 sulfate/anion transporter family, from high endothelial venules and kidney. Genomics 79: 249-256. RECOMMENDED SECONDARY REAGENTS 3. Lohi, H., et al. 2002. Functional characterization of three novel tissue-specif ic To ensure optimal results, the following support (secondary) reagents are anion exchangers SLC26A7, -A8, and -A9. J. Biol. Chem. 277: 14246- 14254. recommended: 1) Western Blotting: use donkey anti-goat IgG-HRP: sc-2020 4. Online Mendelian Inheritance in Man, OMIM™. 2004. Johns Hopkins (dilution range: 1:2000-1:100,000) or Cruz Marker™ compatible donkey University, Baltimore, MD. MIM Number: 608480. World Wide Web URL: anti- goat IgG-HRP: sc-2033 (dilution range: 1:2000-1:5000), Cruz Marker™ http://www.ncbi.nlm.nih.gov/omim/ Molecular Weight Standards: sc-2035, TBS Blotto A Blocking Reagent: sc-2333 and Western Blotting Luminol Reagent: sc-2048. 2) Immunofluo- 5. Mäkelä, S., et al. 2005. Mutational analysis of the human SLC26A8 gene: rescence: use donkey anti-goat IgG-FITC: sc-2024 (dilution range: 1:100- exclusion as a candidate for male infertility due to primary spermatogenic 1:400) or donkey anti-goat IgG-TR: sc-2783 (dilution range: 1:100-1:400) failure. Mol. Hum. Reprod. 11: 129-132. with UltraCruz™ Mounting Medium: sc-24941. 6. Touré, A., et al. 2007. The testis anion transporter 1 (Slc26a8) is required for sperm terminal differentiation and male fertility in the mouse. Hum. RESEARCH USE Mol. Genet. 16: 1783-1793. For research use only, not for use in diagnostic procedures. 7. Lhuillier, P., et al. 2009. Absence of annulus in human asthenozoospermia: case report. Hum. Reprod. 24: 1296-1303. PROTOCOLS See our web site at www.scbt.com or our catalog for detailed protocols and CHROMOSOMAL LOCATION support products. Genetic locus: SLC26A8 (human) mapping to 6p21.31; Slc26a8 (mouse) mapping to 17 A3.3.

SOURCE SLC26A8 (N-15) is an affinity purified goat polyclonal antibody raised against a peptide mapping within an N-terminal cytoplasmic domain of SLC26A8 of human origin.

STORAGE Store at 4° C, **DO NOT FREEZE**. Stable for one year from the date of shipment. Non-hazardous. No MSDS required.

Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com