Human PRC1 (His Tag)

Catalog Number: 13280-H07B

General Information SDS-PAGE:

Gene Name Synonym:

ASE1 Protein Construction:

A DNA sequence encoding the human PRC1 isoform 1 (NP_003972.1) (Met 1-Ser 620) was expressed, with a polyhistidine tag at the N-terminus. Source: Human Expression Host: Baculovirus-Insect Cells QC Testing

Purity: > 95 % as determined by SDS-PAGE Endotoxin: Protein Description < 1.0 EU per μg of the protein as determined by the LAL method PRC1 (protein regulator of 1) is a key regulator of cytokinesis Stability: that cross-links antiparrallel microtubules at an average distance of 35 nM. It is essential for controlling the spatiotemporal formation of the midzone Samples are stable for up to twelve months from date of receipt at -70 ℃ and successful cytokinesis. PRC1 is required for KIF14 localization to the central spindle and midbody. It is also required to recruit PLK1 to the Predicted N terminal: Met spindle. PRC1 stimulates PLK1 phosphorylation of RACGAP1 to allow Molecular Mass: recruitment of ECT2 to the central spindle. It is a homodimer and interacts with the C-terminal Rho-GAP domain and the basic region of RACGAP1. The recombinant human PRC1 consists of 639 amino acids and predicts a The interaction with RACGAP1 inhibits its GAP activity towards CDC42 in molecular mass of 74 kDa. It migrates as an approximately 75 KDa band vitro, which may be required for maintaining normal spindle morphology. in SDS-PAGE under reducing conditions. PRC1 also interacts separately via its N-terminal region with the C-terminus of CENPE, KIF4A and KIF23 during late mitosis. It interacts with KIF14, Formulation: IF20A and PLK1.

Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 20% gly, 3mM References DTT 1.Jiang W, et al. (1999) PRC1: a human mitotic spindle-associated CDK Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as substrate protein required for cytokinesis. Mol Cell. 2(6):877-85. 2.Rual, et protectants before lyophilization. Specific concentrations are included in al. (2005) Towards a proteome-scale map of the human protein-protein the hardcopy of COA. Please contact us for any concerns or special interaction network. Nature. 437(7062):173-8. 3.Mollinari C, et al. (2002) requirements. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J Cell Biol. 15 (7):1175-86. Usage Guide

Storage:

Store it under sterile conditions at -20℃ to -80℃ upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage.

Avoid repeated freeze-thaw cycles.

Reconstitution:

Detailed reconstitution instructions are sent along with the products.

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