Curriculum Vitae P. 1

Curriculum Vitae p. 1

Name: Jakob Balslev Sørensen Title/degrees Privatdozent, Ph.D., M.Sc. Citizenship: Danish. Born: Hørsholm, Denmark on September 24th 1969. Marital status: Married to Christiane E. Sørensen, two children (4 and 8 years). Private address: Augustinerstrasse 30, 37077 Göttingen, Germany. Work address: Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen, Germany. Office phone: +49 (0) 551 201 1297 Private phone: +49 (0) 551 205 2704 Fax: +49 (0) 551 201 1688 E-mail: [email protected]

Education 2006 Privatdozent (December 12th 2006). Venia Legendi (“permission to teach”) in pre-clinical physiology at the Georg-August University of Göttingen. Habilitation supervisor: Prof. Dr. Diethelm Richter, Human Medicin, Göttingen University. (This is the German ‘Habilitation’). 1999 Ph.D. (September 23rd 1999). August Krogh Institute, Copenhagen University. Supervisor: Prof. Dr. Erik H. Larsen. Thesis title: Biophysical studies of ion channels. 1996 M.Sc./cand. scient. (February 14th 1996) in Biology (final and thesis note: 13, i.e. Highest Distinction), and Minor Degree in Mathematics from Copenhagen University, Denmark. 1988 Generel Certificate of Education (A-level): Marie Kruses Skole, Farum, Denmark.

Work experience etc. 2009- Professor of Molecular and Cellular Neurobiology, Institute for Neuroscience and Pharmacology, Faculty of Health, Copenhagen University. 2009- Lektor (associate professor), Institute for Neuroscience and Pharmacology, Faculty of Health, Copenhagen University (from January 1st 2009). 2005-2008 Research Group Leader (current position), Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany. 2000-2005 Post-doctoral fellow, Prof. Dr. Erwin Neher, Membranbiophysik, Max-Planck-Institut für Biophysikalische Chemie, Göttingen, Germany. 1999 Post-doctoral fellow, Prof. Dr. Erik H. Larsen, August Krogh Institute, Copenhagen University. 1998 Visiting scientist (for 6 months) as part of the Ph.D. thesis work with Prof. Dr. Francisco Bezanilla, Department for Anaesthesiology and Physiology, University of California Los Angeles, USA. 1996-1999 Ph.D. student at the August Krogh Institute, Copenhagen University. 1988-1996 Study of Biology (main subject) and Mathematics (minor subject) at Copenhagen University.

Memberships of Ph.D. schools etc. since 2008 Faculty member of ‘Faculty of 1000’, Neuronal Signaling Mechanisms Section. since 2007 Faculty member and member of the study committee for the Ph.D. program ‘Molecular Physiology of the Brain’, Göttingen Graduate School for Neurosciences and Molecular Biosciences. since 2006 Faculty member of the International Max-Planck-Research School in the Neurosciences. since 2006 Faculty member of Georg August School of Science (GAUSS) with the right to act as Ph.D. supervisor and evaluator in the Natural Sciences Faculty, Georg August University Göttingen. since 2005 Faculty member of the NEUREST (neuroscience early stage research training) program, Göttingen. 1991-1993 Student member of the study committee (“Studienævnet”) for Biology, Copenhagen University.

Research grants, stipends, awards Running research grants: 2009-2011 The Danish Medical research council, grant of € 270,000. 2009-2013 The Lundbeck foundation: Junior group leader Scholarship 2008, total of € 1.34 mio.

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(2008 Novo Nordisk Seniorforskerstipendiet 2008, total of € 765,100 over five years. The stipend has been returned to the foundation due to the overlapping grant from Lundbeckfonden). 2008-2011 “Scientist in charge” for a Marie Curie IEF FP7 stipend and an EMBO long-term fellowship, both given to Keimpe Wierda, Post.-doc. in my group since Oktober 2007. 2006-2008 Deutsche Forschungsgemeinschaft: ‘Special Research Area 523’ (SFB523), project B16: one Ph.D. student and €8000/year for three years. Total amount: approx. € 108,800. 2006-2008 Deutsche Forschungsgemeinschaft: ‘Graduate College 521’ (GRK521): one Ph.D. student and €8000/year for three years. Total amount: approx. € 64,000.

Previous research grants, prices: 2005-2007 Deutsche Forschungsgemeinscaft: „The own position” SO 708/1-1&2 and €3500/year. Total amount: Approx. € 170,000. 2004-2005 ‘Scientist in charge’ for an EMBO long-term fellowship given to Jean-Sebastien Schonn. 2000 Danisch Research council for the Natural Sciences: relocation stipend. 1996 Gold medal, Copenhagen University 1994-1996 Novo Nordic Student Scholarship.

Reviewer for Nature Cell Biology, PLOS Biology, EMBO Journal, Journal of Cell Biology, Journal of Neuroscience, Journal of General Physiology, Journal of Physiology, Biophysical Journal, Brain Research, American Journal of Physiology, Journal of Neurochemistry, Chemical Reviews, Proceedings of the National Academy of Sciences (USA), Homburger Forschungsförderungsprogramm (HOMFOR), Deutsche Forschungsgemeinschaft, INTAS u.a.

Collaborations Prof. Thomas C. Südhof, Department of Neurosciences, The University of Texas Southwestern Medical Center, Dallas, Texas 75390, USA. Themes: Synaptotagmin isoforms, knock-out mice. Prof. Nils Brose, Max-Planck-Institute for experimental medicine, Hermann-Rein-Str. 3, 37075 Göttingen, Germany. Themes: knock-out mice, biochemistry. Prof. Matthijs Verhage, Department of Functional Genomics, Vrije Universiteit Amsterdam, Amsterdam, Holland. Themes: Munc18-1, knock-out mice, electron microscopy. Prof. Manfred Lindau, School of Applied and Engineering Physics, Cornell University, Ithaca, NY 14853, USA. Themes: fusion pore measurements, synaptobrevin mutations. Prof. Graham Ellis-Davies, Department of Pharmacology and Physiology, Drexel University College of Medicine, 245 N. 15th Street, Philadelphia, PA 19102, USA. Themes: calcium cages. Dr. Dirk Fasshauer, Forschungsgruppenleiter, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen. Themes: biochemical measurements of SNAREs. Prof. Reinhard Jahn, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen. Themes: biochemical measurements of SNAREs. Prof. Erwin Neher, Max-Planck-Institut für biophysikalische Chemie, Am Fassberg 11, 37077 Göttingen. Themes: fluctuation analysis, deconvolution analysis, etc.

Memberships American Physiological Society (Member since 2000). Society for Neuroscience (Member since 2002). Biophysical Society (Member since 2003). Society for General Physiologists (Member since 2004). Neurowissenschaftliche Gesellschaft (Member since 2005).

Curriculum Vitae p. 3

Publications

1. Research papers

28. Cai H., Reim K., Varoqueaux F., Tapechum S., Hill K., Sørensen J.B., Brose N., and Chow R.H. (2008 – in press). Complexin II plays a positive role in Ca2+-triggered exocytosis by facilitating vesicle priming. Proc. Natl. Acad. Sci. (USA)

27. Fang Q., Berberian K., Gong L.W., Hafez I., Sørensen J.B., and Lindau M. (2008). The role of the C terminus of the SNARE protein SNAP-25 in fusion pore opening and a model for fusion pore mechanics. Proc. Natl. Acad. Sci. (USA) 105(40), 15388-15392.

26. Nagy G., Milosevic I., Mohrman, R., Wiederhold K., Walter A.M., and Sørensen J.B. (2008). The SNAP-25 linker as an adaptation towards fast exocytosis. Mol. Biol. Cell 19, 3769-3781.

25. Schonn J.S., Maximov A., Lao Y., Südhof T.C., and Sørensen J.B. (2008). Synaptotagmin-7 and -1 are functionally overlapping calcium sensors for exocytosis in adrenal chromaffin cells. Proc. Natl. Acad. Sci. (USA) 105, 3998-4003.

24. Maximov A., Lao Y., Li H., Chen X., Rizo J., Sørensen J.B., and Südhof T.C. (2008). Genetic analysis of synaptotagmin-7 function in synaptic vesicle exocytosis. Proc. Natl. Acad. Sci. (USA) 105, 3986-3991.

23. Jockusch W.J., Speidel D., Sigler A., Sørensen J.B., Varoqueaux F., Rhee J.-S, and Brose N. (2007). CAPS-1 and CAPS-2 are essential synaptic vesicle priming proteins. Cell 131, 796-808.

22. Delgado-Martinez I., Nehring R., and Sørensen J.B. (2007). Differential abilities of SNAP-25 homologues to support neuronal function. J. Neurosci. 27, 9380-9391.

21. Gulyás-Kovács A., de Wit H., Kochubey O., Milosevic I., Toonen R., Klingauf J., Verhage M., and Sørensen J.B. (2007). Munc18-1: sequential interactions with the fusion machinery stimulate vesicle docking and priming. J. Neurosci. 27, 8676-8686.

20. Zilly F., Sørensen J.B., Jahn R., and Lang T. (2006). Munc-18-1 binding to syntaxin 1 allows for the initiation of the neuronal SNARE-zipper in native membranes. PLoS Biology Sep 26; 4(10):e330 doi:10.1371/journal.pbio.0040330.

19. Nili U., de Wit H., Gulyás-Kovács A., Toonen R.F., Sørensen J.B., Verhage M., and Ashery U. (2006). Munc18-1 phosphorylation by PKC potentiates vesicle pool replenishment in bovine chromaffin cells. Neuroscience 143, 487-500.

18. Toonen R.F., Kochubey O., de Wit H., Gulyas-Kovacs A., Konijnenburg B., Sørensen J.B., Klingauf J., and Verhage M. (2006). Dissecting docking and tethering of secretory vesicles at the target membrane. EMBO J. 25, 3725-3737.

17. Sørensen J.B., Wiederhold K., Müller E.M., Milosevic I., Nagy G., de Groot B.L., Grubmüller H., and Fasshauer D. (2006). Sequential N- to C-terminal SNARE complex assembly drives priming and fusion of secretory vesicles. EMBO J. 25, 955-966.

16. Nagy G., Kim J.H., Pang Z.P., Matti U., Rettig J., Sudhof T.C., Sørensen J.B. (2006). Different effects on fast exocytosis induced by synaptotagmin 1 and 2 isoforms and abundance but not by phosphorylation. J Neurosci. 26, 632-643.

Curriculum Vitae p. 4

15. Nagy G., Milosevic I., Fasshauer D., Muller E.M., de Groot B.L., Lang T., Wilson M.C., and Sørensen J.B. (2005). Alternative Splicing of SNAP-25 Regulates Secretion through Nonconservative Substitutions in the SNARE Domain. Mol. Biol. Cell 16, 5675-5685

14. Milosevic I., Sørensen J.B., Lang T., Krauss M., Nagy G., Haucke V., Jahn R., and Neher E. (2005). Plasmalemmal PI(4,5)P2 level regulates the releasable vesicle pool size in chromaffin cells. J. Neurosci. 25, 2557-2565.

13. Nagy G., Reim K., Matti U., Brose N., Binz T., Rettig J., Neher E., and Sørensen,J.B. (2004). Regulation of releasable vesicle pool sizes by protein kinase A-dependent phosphorylation of SNAP- 25. Neuron 41, 351-365.

12. Sørensen J.B., Fernandez-Chacon R., Südhof T.C., and Neher E. (2003). Examining synaptotagmin I function in dense core vesicle exocytosis under direct control of Ca2+. J. Gen. Physiol. 122, 265-276.

11. Sørensen J.B., Nagy G., Varoqueaux F., Nehring R.B., Brose N., Wilson M.C., and Neher E. (2003). Differential control of the releasable vesicle pools by SNAP-25 splice variants and SNAP-23. Cell 114, 75-86. (Joint first-authorship)

10. Brede M., Nagy G., Phillip M., Sørensen J.B., Lohse M.J., and Hein L. (2003). Differential control of adrenal and sympathetic catecholamine release by α2-receptor subtypes. Mol. Endocrinol. 17, 1640-1646.

9. Nagy G., Matti U., Nehring R.B., Binz T., Rettig J., Neher E., and Sørensen J.B. (2002) Protein kinase C-dependent phosphorylation of synaptosome-associated protein of 25 kDa at Ser187 potentiates vesicle recruitment. J. Neurosci. 22, 9278-9286.

8. Sørensen J.B., Matti U., Wei S., Nehring R.B., Voets T., Ashery U., Binz T., Neher E., and Rettig J. (2002). The SNARE protein SNAP-25 is linked to fast calcium-triggering of exocytosis. Proc Natl Acad Sci U S A. 99, 1627-1632.

7. Sørensen J.B., Nielsen M.S., Gudme C.N., Larsen E.H., and Nielsen R. (2001). Maxi K+ channels co-localised with CFTR in the apical membrane of an exocrine gland acinus: Possible involvement in macroscopic secretion. Pflügers Arch. - Eur. J. Physiol. 442, 1-11. (Joint first-authorship)

6. Larsen E.H., Sørensen J.B., and Sørensen J.N. (2000). A mathematical model of solute coupled water transport in leaky epithelia incorporating recirculation of the actively transported solute. J. Gen. Physiol. 116, 101-124.

5. Sørensen J.B., Cha A., Latorre R., Rosenmann E., and Bezanilla F. (2000). Deletion of the S3-S4 linker in the Shaker potassium channel reveals two quenching groups outside S4. J. Gen. Physiol. 115, 209-221.

4. Sørensen J.B., and Larsen E.H. (1999). Membrane potential and conductance of frog skin gland acinar cells in resting conditions and during stimulation with agonists of macroscopic secretion. Pflügers Arch. - Eur. J. Physiol. 439, 101-112.

3. Sørensen J.B., and Larsen E.H. (1998). Patch-clamp on the luminal membrane of exocrine gland acini from frog skin (Rana esculenta) reveals the presence of cystic fibrosis transmembrane conductance regulator-like Cl- channel activated by cAMP. J. Gen. Physiol. 112, 19-31.

2. Sørensen J.B., and Larsen E.H. (1996). Heterogeneity of chloride channels in the apical membrane of isolated mitochondria-rich cells from toad skin. J. Gen. Physiol. 108, 421-433.

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1. Bildsøe M., and Sørensen J.B. (1994). A method of modelling time dependent data: swimming in guppies (Poecilia reticulata) under threat of a predator. Behavioural Processes 31, 75-96.

2. Reviews

Sørensen J.B. (2009 – submitted). Conflicting views on the membrane fusion machinery and the fusion pore. Annu. Rev. Cell Dev. Biol.

6. Sørensen J.B., and Verhage, M. (2008). Vesicle docking in regulated exocytosis. Traffic 9, 1414- 1424.

5. Sørensen J.B. (2005). SNARE complexes prepare for membrane fusion. Trends Neurosci. 28, 453-455.

4. Sørensen J.B. (2004). Formation, stabilisation and fusion of the readily-releasable pool of secretory vesicles. Pflügers Arch. - Eur. J. Physiol. 448, 347-362.

3. Larsen E.H., Sørensen J.B., and Sørensen, J.N. (2002). Analysis of the sodium recirculation theory of solute coupled water transport in small intestine. J. Physiol. 542, 33-50.

2. Larsen E.H., Christoffersen B.C., Jensen L.J., Sørensen J.B. & Willumsen N.J. (1996). Role of mitochondria-rich cells for epithelial chloride uptake. Exp Physiol. 81, 525-534.

1. Larsen E.H., Jensen L.J., Jespersen Å., Møbjerg N., Sørensen J.B., and Willumsen N.J. (1996). Chloride channels of mitochondria-rich cells in anuran skin: Physiological significance and regulation. Zoology 99, 227-236.

3. Book chapters

1. Sørensen J.B. (2008 – in press). “Vesicle pools”. Chapter 1384 in: New Encyclopedia for Neuroscience, edited by Larry Squire et al., Elsevier, Oxford, UK.

4. Other publications

7. Sørensen J.B. (2008) Neuronal communication: how can it be so fast? MPIbpc News 14. Jahrgang, Ausgabe Nr. 1 January 2008.

6. Sørensen J.B. (2006) Untersuchungen zur molekularen Grundlage der Ca++-abhängigen Katecholaminsekretion von Nebennierenchromaffinzellen. Habilitationsschrift. Die medizinische Fakultät der Georg-August-Universität zu Göttingen.

5. Sørensen J.B. (2006) Caged-calcium and its measurement: exocytosis in a flash. In: Kavli Workshop in Pucón: advanced techniques to study single vesicle exocytosis. E-breefing, http://www.nyas.org/ebriefreps/main.asp?intSubSectionID=3939.

4. Sørensen J.B., and Nagy G. (2004) Regulatorische Proteine sorgen für flexible Kommunikation. MPIbpc News 10. Jahrgang, Ausgabe Nr. 3 März 2004 [in the German language].

3. Sørensen J.B. (1999). Biophysical studies of ion channels. Ph.D. thesis. The Faculty of Natural Sciences, University of Copenhagen.

2. Møbjerg N., Sørensen J.B., Jensen L.J., Jespersen Å., Willumsen N.J., and Larsen E.H. (1998). Padders vand- og saltbalance. Naturens Verden 1998/5, 173-187. [in the Danish language]

Curriculum Vitae p. 6

1. Sørensen J.B., Nielsen M.S., Nielsen R., and Larsen E.H. (1998). Luminal ion channels involved in isotonic secretion by Na+-recycling in exocrine gland-acini. Biol. Skr. Dan. Vid. Selsk. 49, 166-178.