CDH4 Polyclonal Antibody

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CDH4 polyclonal antibody calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a Catalog Number: PAB1864 transmembrane region and a highly conserved cytoplasmic tail. Based on studies in chicken and Regulatory Status: For research use only (RUO) mouse, this cadherin is thought to play an important role during brain segmentation and neuronal outgrowth. In Product Description: Rabbit polyclonal antibody raised addition, a role in kidney and muscle development is against synthetic peptide of CDH4. indicated. Of particular interest are studies showing stable cis-heterodimers of cadherins 2 and 4 in Immunogen: A synthetic peptide (conjugated with KLH) cotransfected cell lines. Previously thought to interact in corresponding to N-terminus of human CDH4. an exclusively homophilic manner, this is the first evidence of cadherin heterodimerization. [provided by Host: Rabbit RefSeq] Reactivity: Human References: Applications: Flow Cyt, IF, IHC-P, WB-Ce 1. CDH4 is a novel determinant of osteosarcoma (See our web site product page for detailed applications tumorigenesis and metastasis. Tang Q, Lu J, Zou C, information) Shao Y, Chen Y, Narala S, Fang H, Xu H, Wang J, Shen J, Khokha R. Oncogene. 2018 Apr 3. [Epub ahead of Protocols: See our web site at print] http://www.abnova.com/support/protocols.asp or product 2. Frequent aberrant methylation of the CDH4 gene page for detailed protocols promoter in human colorectal and gastric cancer. Miotto E, Sabbioni S, Veronese A, Calin GA, Gullini S, Liboni A, Form: Liquid Gramantieri L, Bolondi L, Ferrazzi E, Gafa R, Lanza G, Negrini M. Cancer Res. 2004 Nov 15;64(22):8156-9. Purification: Ammonium sulfate precipitation 3. R-cadherin influences cell motility via Rho family GTPases. Johnson E, Theisen CS, Johnson KR, Recommend Usage: Western Blot (1:1000) Wheelock MJ. J Biol Chem. 2004 Jul Immunohistochemistry (1:10-50) 23;279(30):31041-9. Epub 2004 May 13. Immunofluorescence (1:10-50) Flow cytometry (1:10-50) The optimal working dilution should be determined by the end user. Storage Buffer: In PBS (0.09% sodium azide) Storage Instruction: Store at 4°C. For long term storage store at -20°C. Aliquot to avoid repeated freezing and thawing. Entrez GeneID: 1002 Gene Symbol: CDH4 Gene Alias: CAD4, FLJ22202, FLJ40547, MGC126700, MGC138355, RCAD Gene Summary: This gene is a classical cadherin from the cadherin superfamily. The encoded protein is a Page 1/1 Powered by TCPDF (www.tcpdf.org).

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Supplementary Table 1: Adhesion Genes Data Set
Supplementary Table 1: Adhesion genes data set PROBE Entrez Gene ID Celera Gene ID Gene_Symbol Gene_Name 160832 1 hCG201364.3 A1BG alpha-1-B glycoprotein 223658 1 hCG201364.3 A1BG alpha-1-B glycoprotein 212988 102 hCG40040.3 ADAM10 ADAM metallopeptidase domain 10 133411 4185 hCG28232.2 ADAM11 ADAM metallopeptidase domain 11 110695 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 195222 8038 hCG40937.4 ADAM12 ADAM metallopeptidase domain 12 (meltrin alpha) 165344 8751 hCG20021.3 ADAM15 ADAM metallopeptidase domain 15 (metargidin) 189065 6868 null ADAM17 ADAM metallopeptidase domain 17 (tumor necrosis factor, alpha, converting enzyme) 108119 8728 hCG15398.4 ADAM19 ADAM metallopeptidase domain 19 (meltrin beta) 117763 8748 hCG20675.3 ADAM20 ADAM metallopeptidase domain 20 126448 8747 hCG1785634.2 ADAM21 ADAM metallopeptidase domain 21 208981 8747 hCG1785634.2|hCG2042897 ADAM21 ADAM metallopeptidase domain 21 180903 53616 hCG17212.4 ADAM22 ADAM metallopeptidase domain 22 177272 8745 hCG1811623.1 ADAM23 ADAM metallopeptidase domain 23 102384 10863 hCG1818505.1 ADAM28 ADAM metallopeptidase domain 28 119968 11086 hCG1786734.2 ADAM29 ADAM metallopeptidase domain 29 205542 11085 hCG1997196.1 ADAM30 ADAM metallopeptidase domain 30 148417 80332 hCG39255.4 ADAM33 ADAM metallopeptidase domain 33 140492 8756 hCG1789002.2 ADAM7 ADAM metallopeptidase domain 7 122603 101 hCG1816947.1 ADAM8 ADAM metallopeptidase domain 8 183965 8754 hCG1996391 ADAM9 ADAM metallopeptidase domain 9 (meltrin gamma) 129974 27299 hCG15447.3 ADAMDEC1 ADAM-like,
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Supplementary Material
Supplementary Material Table S1: Significant downregulated KEGGs pathways identified by DAVID following exposure to five cinnamon- based phenylpropanoids (p < 0.05). p-value Term: Genes (Benjamini) Cytokine-cytokine receptor interaction: FASLG, TNFSF14, CXCL11, IL11, FLT3LG, CCL3L1, CCL3L3, CXCR6, XCR1, 2.43 × 105 RTEL1, CSF2RA, TNFRSF17, TNFRSF14, CCNL2, VEGFB, AMH, TNFRSF10B, INHBE, IFNB1, CCR3, VEGFA, CCR2, IL12A, CCL1, CCL3, CXCL5, TNFRSF25, CCR1, CSF1, CX3CL1, CCL7, CCL24, TNFRSF1B, IL12RB1, CCL21, FIGF, EPO, IL4, IL18R1, FLT1, TGFBR1, EDA2R, HGF, TNFSF8, KDR, LEP, GH2, CCL13, EPOR, XCL1, IFNA16, XCL2 Neuroactive ligand-receptor interaction: OPRM1, THRA, GRIK1, DRD2, GRIK2, TACR2, TACR1, GABRB1, LPAR4, 9.68 × 105 GRIK5, FPR1, PRSS1, GNRHR, FPR2, EDNRA, AGTR2, LTB4R, PRSS2, CNR1, S1PR4, CALCRL, TAAR5, GABRE, PTGER1, GABRG3, C5AR1, PTGER3, PTGER4, GABRA6, GABRA5, GRM1, PLG, LEP, CRHR1, GH2, GRM3, SSTR2, Chlorogenic acid Chlorogenic CHRM3, GRIA1, MC2R, P2RX2, TBXA2R, GHSR, HTR2C, TSHR, LHB, GLP1R, OPRD1 Hematopoietic cell lineage: IL4, CR1, CD8B, CSF1, FCER2, GYPA, ITGA2, IL11, GP9, FLT3LG, CD38, CD19, DNTT, 9.29 × 104 GP1BB, CD22, EPOR, CSF2RA, CD14, THPO, EPO, HLA-DRA, ITGA2B Cytokine-cytokine receptor interaction: IL6ST, IL21R, IL19, TNFSF15, CXCR3, IL15, CXCL11, TGFB1, IL11, FLT3LG, CXCL10, CCR10, XCR1, RTEL1, CSF2RA, IL21, CCNL2, VEGFB, CCR8, AMH, TNFRSF10C, IFNB1, PDGFRA, EDA, CXCL5, TNFRSF25, CSF1, IFNW1, CNTFR, CX3CL1, CCL5, TNFRSF4, CCL4, CCL27, CCL24, CCL25, CCL23, IFNA6, IFNA5, FIGF, EPO, AMHR2, IL2RA, FLT4, TGFBR2, EDA2R,
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Human R-Cadherin / CDH4 Protein (His Tag)
Human R-Cadherin / CDH4 Protein (His Tag) Catalog Number: 10230-H08H General Information SDS-PAGE: Gene Name Synonym: CAD4; R-CAD; RCAD Protein Construction: A DNA sequence encoding the extracellular domain of human CAD4 (NP_001785.2) (Met 1-Ala 734) was expressed with a fused polyhistidine tag at the C-terminus. Source: Human Expression Host: HEK293 Cells QC Testing Purity: > 85 % as determined by SDS-PAGE Endotoxin: Protein Description < 1.0 EU per μg of the protein as determined by the LAL method The cadherin superfamily is a large family that engage in both homo- and heterotypic, calcium-dependent, cell-cell adhesion events, and can be Stability: divided into at least four subfamilies based on the extracellular (EC) regions and cytoplasmic domains, that is: classical cadherins, desmosomal Samples are stable for up to twelve months from date of receipt at -70 ℃ cadherins, protocadherins, and cadherin-like molecules. Human cadherin 4, type 1, R-cadherin (retinal), also known as CDH4, CAD4 and RCAD, is a Predicted N terminal: His 21 classical cadherin from the cadherin superfamily. It is a calcium-dependent Molecular Mass: adhesion molecule and a type I transmembrane glycoprotein composed of five extracellular cadherin repeats, a transmembrane region and a highly The recombinant pro form of human CAD4 comprises 725 amino acids and conserved cytoplasmic tail. CDH4 is thought to play an important role predicts a molecular mass of 80 kDa. As a result of glycosylation, rhCAD4 during brain segmentation and neuronal outgrowth, and also exerts critical migrates as an approximately 90-100 kDa protein in SDS-PAGE under actions in kidney and muscle development.
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Study on Expression of CDH4 in Lung Cancer Zhupeng Li1, Dan Su2, Lisha Ying2, Guangmao Yu1 and Weimin Mao3*
Li et al. World Journal of Surgical Oncology (2017) 15:26 DOI 10.1186/s12957-016-1083-2 RESEARCH Open Access Study on expression of CDH4 in lung cancer Zhupeng Li1, Dan Su2, Lisha Ying2, Guangmao Yu1 and Weimin Mao3* Abstract Background: The human CDH4 gene, which encodes the R-cadherin protein, has an important role in cell migration and cell adhesion, sorting, tissue morphogenesis, and tumor genesis. This study analyzed the relationship of CDH4 mRNA expression with lung cancer. Methods: Real time PCR was applied to detect CDH4 mRNA transcription in 142 paired cases of lung cancer and noncancerous regions. Results: No correlation was identified between CDH4 mRNA expression and gender, age, lymphnode metastasis, TNM stage, family history, smoking state, drinking state (P > 0.05), but grade and histotype (P < 0.05). The relative CDH4 mRNA value was remarkably decreased in lung cancer tissues compared with noncancerous tissues (P =0.001). Conclusions: We found that CDH4 mRNA expression was associated with grade and histotype. What is more, therelativeCDH4mRNAvaluewasdecreasedinthelung cancer tissues. Our results suggested that CDH4 might be a putative tumor suppressor gene (TSG) in lung cancer. Keywords: CDH4, Lung cancer, Tumor suppressor gene Background R-cadherin is a classic cadherin, which is important Lung cancer has the highest morbidity and mortality in for the differentiation of kidney, striated muscle, brain, malignant tumors and with a total of 1.5 million deaths and so on [6, 7]. R-cadherin plays a critical role in main- annually worldwide [1]. In the patients with lung cancer, taining cell polarity and tissue architecture in normal though diagnosis and treatment have been greatly gastrointestinal epithelial tissue [8].
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Cell–Cell Adhesion Molecules During Neocortical Development
cells Review Stick around: Cell–Cell Adhesion Molecules during Neocortical Development David de Agustín-Durán † , Isabel Mateos-White † , Jaime Fabra-Beser and Cristina Gil-Sanz * Neural Development Laboratory, Instituto Universitario de Biomedicina y Biotecnología (BIOTECMED) and Departamento de Biología Celular, Facultat de Biología, Universidad de Valencia, 46100 Burjassot, Spain; [email protected] (D.d.A.-D.); [email protected] (I.M.-W.); [email protected] (J.F.-B.) * Correspondence: [email protected]; Tel.: +34-96-354-4173 † These authors contributed equally to this work. Abstract: The neocortex is an exquisitely organized structure achieved through complex cellular processes from the generation of neural cells to their integration into cortical circuits after complex migration processes. During this long journey, neural cells need to establish and release adhesive interactions through cell surface receptors known as cell adhesion molecules (CAMs). Several types of CAMs have been described regulating different aspects of neurodevelopment. Whereas some of them mediate interactions with the extracellular matrix, others allow contact with additional cells. In this review, we will focus on the role of two important families of cell–cell adhesion molecules (C-CAMs), classical cadherins and nectins, as well as in their effectors, in the control of fundamental processes related with corticogenesis, with special attention in the cooperative actions among the two families of C-CAMs. Keywords: CAMs; classical cadherins; nectins; neocortical development; radial glia cells; neurons; neuronal migration; axon targeting; synaptogenesis; neurodevelopmental disorders Citation: de Agustín-Durán, D.; Mateos-White, I.; Fabra-Beser, J.; 1. Introduction Gil-Sanz, C. Stick around: Cell–Cell The nervous system structure and functionality are sustained by an exceptionally Adhesion Molecules during complex network of interconnected cells.
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Supplementary Table 2
Ayrault et al. Supplementary Table S2 Term Count % PValue GO:0007155~cell adhesion 55 13.06% 3.97E-15 1417231_at Cldn2 11.31 CLAUDIN 2 1418153_at Lama1 8.17 LAMININ, ALPHA 1 1434601_at Amigo2 7.78 ADHESION MOLECULE WITH IG LIKE DOMAIN 2 1456214_at Pcdh7 7.26 PROTOCADHERIN 7 1437442_at Pcdh7 6.41 PROTOCADHERIN 7 1445256_at Vcl 6.36 VINCULIN 1427009_at Lama5 5.7 LAMININ, ALPHA 5 1453070_at Pcdh17 5.17 PROTOCADHERIN 17 1428571_at Col9a1 4.59 PROCOLLAGEN, TYPE IX, ALPHA 1 1416039_x_at Cyr61 4.56 CYSTEINE RICH PROTEIN 61 1437932_a_at Cldn1 4.53 CLAUDIN 1 1456397_at Cdh4 4.44 CADHERIN 4 1427010_s_at Lama5 4.23 LAMININ, ALPHA 5 1449422_at Cdh4 3.71 CADHERIN 4 1452784_at Itgav 3.01 INTEGRIN ALPHA V 1435603_at Sned1 2.87 SUSHI, NIDOGEN AND EGF-LIKE DOMAINS 1 1438928_x_at Ninj1 2.85 NINJURIN 1 1441498_at Ptprd 2.69 PROTEIN TYROSINE PHOSPHATASE, RECEPTOR TYPE, D 1419632_at Tecta -33.82 TECTORIN ALPHA 1455056_at Lmo7 -14.83 LIM DOMAIN ONLY 7 1450637_a_at Aebp1 -10.7 AE BINDING PROTEIN 1 1421811_at Thbs1 -10.27 THROMBOSPONDIN 1 1434667_at Col8a2 -8.51 PROCOLLAGEN, TYPE VIII, ALPHA 2 1418304_at Pcdh21 -7.26 PROTOCADHERIN 21 1451454_at Pcdh20 -6.63 PROTOCADHERIN 20 1424701_at Pcdh20 -6.54 PROTOCADHERIN 20 1451758_at Lamc3 -6.15 LAMININ GAMMA 3 1422514_at Aebp1 -5.74 AE BINDING PROTEIN 1 1422694_at Ttyh1 -5.62 TWEETY HOMOLOG 1 (DROSOPHILA) 1425594_at Lamc3 -5.43 LAMININ GAMMA 3 1431152_at Hapln3 -5.39 HYALURONAN AND PROTEOGLYCAN LINK PROTEIN 3 1418424_at Tnfaip6 -5.17 TUMOR NECROSIS FACTOR ALPHA INDUCED PROTEIN 6 1451769_s_at Pcdha4;pcdha10;pcdha1;pcdha2;pcdhac1;pcdha6;pcdha8;pcdhac2;pcdha5;pcdha7;pcdha9;pcdha12;pcdha3-4.99
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Cadherins Interact with Synaptic Organizers to Promote Synaptic Differentiation
Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation The Harvard community has made this article openly available. Please share how this access benefits you. Your story matters Citation Yamagata, Masahito, Xin Duan, and Joshua R. Sanes. 2018. “Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation.” Frontiers in Molecular Neuroscience 11 (1): 142. doi:10.3389/fnmol.2018.00142. http://dx.doi.org/10.3389/ fnmol.2018.00142. Published Version doi:10.3389/fnmol.2018.00142 Citable link http://nrs.harvard.edu/urn-3:HUL.InstRepos:37160429 Terms of Use This article was downloaded from Harvard University’s DASH repository, and is made available under the terms and conditions applicable to Other Posted Material, as set forth at http:// nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of- use#LAA ORIGINAL RESEARCH published: 30 April 2018 doi: 10.3389/fnmol.2018.00142 Cadherins Interact With Synaptic Organizers to Promote Synaptic Differentiation Masahito Yamagata, Xin Duan † and Joshua R. Sanes* Center for Brain Science and Department of Molecular and Cellular Biology, Harvard University, Cambridge, MA, United States Classical cadherins, a set of 20 related recognition and signaling molecules, have ∼ been implicated in many aspects of neural development, including the formation and remodeling of synapses. Mechanisms underlying some of these steps have been studied by expressing N-cadherin (cdh2), a Type 1 cadherin, in heterologous cells, but analysis is complicated because widely used lines express cdh2 endogenously. We used CRISPR-mediated gene editing to generate a Human embryonic kidney (HEK)293 variant lacking Cdh2, then compared the behavior of rodent cortical and hippocampal neurons co-cultured with parental, cdh2 mutant and cdh2-rescued 293 lines.
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The Central Role of Cadherins in Gonad Development, Reproduction and Fertility
Preprints (www.preprints.org) | NOT PEER-REVIEWED | Posted: 2 October 2020 doi:10.20944/preprints202010.0037.v1 Review The central role of cadherins in gonad development, reproduction and fertility Rafal P. Piprek 1,*, Malgorzata Kloc 2,3,4, Paulina Mizia 1 and Jacek Z. Kubiak 5,6,* 1 Department of Comparative Anatomy, Institute of Zoology and Biomedical Research, Jagiellonian University, Gronostajowa 9, 30-387 Krakow, Poland; e-mail [email protected] (R.P.P.), [email protected] (P.M.) 2 The Houston Methodist Research Institute, Houston, TX 77030, USA; e-mail [email protected] 3 Department of Surgery, The Houston Methodist Hospital, Houston, TX 77030, USA 4 MD Anderson Cancer Center, University of Texas, Houston, TX 77030, USA 5 UnivRennes, UMR 6290 CNRS/UR1, Institute of Genetics and Development of Rennes, Cycle Group, Faculty of Medicine, F-35000 Rennes, France; e-mail [email protected] 6 Department of Regenerative Medicine and Cell Biology, Military Institute of Hygiene and Epidemiology (WIHE), 01-163 Warsaw, Poland * Correspondence: [email protected]; +330612253086 (J.Z.K) [email protected]; Tel.: +48126645059 (R.P.P.) Abstract: Cadherins are a group of membrane proteins responsible for cell adhesion. They are crucial for cell sorting and recognition during the morphogenesis, but also play many other roles such as assuring tissue integrity and resistance to stretching, mechanotransduction, cell signaling, regulation of cell proliferation, apoptosis, survival, carcinogenesis, etc. Within the cadherin superfamily, the E- and N-cadherin have been especially well studied. They are involved in many aspects of sexual development and reproduction, such as germline development and gametogenesis, gonad development and functioning, and fertilization.
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Cryβb2 Enhances Tumorigenesis Through Upregulation of Nucleolin in Triple Negative Breast Cancer
www.nature.com/onc Oncogene ARTICLE CRYβB2 enhances tumorigenesis through upregulation of nucleolin in triple negative breast cancer Yu Yan 1,2,3, Athira Narayan1, Soonweng Cho3, Zhiqiang Cheng4, Jun O. Liu 3,4, Heng Zhu 4, Guannan Wang3, Bryan Wharram 1, Ala Lisok1, Mary Brummet1, Harumi Saeki 5,6, Tao Huang2, Kathleen Gabrielson3,5,6, Edward Gabrielson6, Leslie Cope3, 7 8 8 9 10 1,3,4 Yasmine M. Kanaan , Ali✉ Afsari , Tammey Naab , Harris✉ G. Yfantis , Stefan Ambs , Martin G. Pomper , Saraswati Sukumar 3 and Vanessa F. Merino 1 © The Author(s), under exclusive licence to Springer Nature Limited 2021 Expression of β-crystallin B2 (CRYβB2) is elevated in African American (AA) breast tumors. The underlying mechanisms of CRYβB2- induced malignancy and the association of CRYβB2 protein expression with survival have not yet been described. Here, we report that the expression of CRYβB2 in breast cancer cells increases stemness, growth, and metastasis. Transcriptomics data revealed that CRYβB2 upregulates genes that are functionally associated with unfolded protein response, oxidative phosphorylation, and DNA repair, while down-regulating genes related to apoptosis. CRYβB2 in tumors promotes de-differentiation, an increase in mesenchymal markers and cancer-associated ﬁbroblasts, and enlargement of nucleoli. Proteome microarrays identiﬁed a direct interaction between CRYβB2 and the nucleolar protein, nucleolin. CRYβB2 induces nucleolin, leading to the activation of AKT and EGFR signaling. CRISPR studies revealed a dependency on nucleolin for the pro-tumorigenic effects of CRYβB2. Triple-negative breast cancer (TNBC) xenografts with upregulated CRYβB2 are distinctively sensitive to the nucleolin aptamer, AS-1411. Lastly, in AA patients, higher levels of nucleolar CRYβB2 in primary TNBC correlates with decreased survival.
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E-Cadherin Loss Alters Cytoskeletal Organization and Adhesion in Non-Malignant Breast Cells but Is Insufficient to Induce An
Chen et al. BMC Cancer 2014, 14:552 http://www.biomedcentral.com/1471-2407/14/552 RESEARCH ARTICLE Open Access E-cadherin loss alters cytoskeletal organization and adhesion in non-malignant breast cells but is insufficient to induce an epithelial-mesenchymal transition Augustine Chen1, Henry Beetham1, Michael A Black1, Rashmi Priya2, Bryony J Telford1, Joanne Guest1, George A R Wiggins1, Tanis D Godwin1, Alpha S Yap2 and Parry J Guilford1* Abstract Background: E-cadherin is an adherens junction protein that forms homophilic intercellular contacts in epithelial cells while also interacting with the intracellular cytoskeletal networks. It has roles including establishment and maintenance of cell polarity, differentiation, migration and signalling in cell proliferation pathways. Its downregulation is commonly observed in epithelial tumours and is a hallmark of the epithelial to mesenchymal transition (EMT). Methods: To improve our understanding of how E-cadherin loss contributes to tumorigenicity, we investigated the impact of its elimination from the non-tumorigenic breast cell line MCF10A. We performed cell-based assays and whole genome RNAseq to characterize an isogenic MCF10A cell line that is devoid of CDH1 expression due to an engineered homozygous 4 bp deletion in CDH1 exon 11. Results: The E-cadherin-deficient line, MCF10A CDH1-/- showed subtle morphological changes, weaker cell-substrate adhesion, delayed migration, but retained cell-cell contact, contact growth inhibition and anchorage-dependent growth. Within the cytoskeleton, the apical microtubule network in the CDH1-deficient cells lacked the radial pattern of organization present in the MCF10A cells and F-actin formed thicker, more numerous stress fibres in the basal part of the cell.
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Pan-Cancer Genomic Amplifications Underlie a Wnt Hyperactivation Phenotype
bioRxiv preprint doi: https://doi.org/10.1101/519611; this version posted January 13, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Pan-cancer genomic amplifications underlie a Wnt hyperactivation phenotype 2 associated with stem cell-like features leading to poor prognosis 3 4 5 6 Wai Hoong Chang and Alvina G. Lai 7 8 9 Nuffield Department of Medicine, University of Oxford, 10 Old Road Campus, Oxford, OX3 7FZ, United Kingdom 11 12 For correspondence: [email protected] bioRxiv preprint doi: https://doi.org/10.1101/519611; this version posted January 13, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 13 List of Abbreviations 14 TCGA The Cancer Genome Atlas KEGG Kyoto Encyclopedia of Genes and Genomes GO Gene Ontology ROC Receiver operating characteristic AUC Area under the curve HR Hazard ratio TNM Tumor, node and metastasis HIF Hypoxia inducible factor TF Transcription factor EMT Epithelial-to-mesenchymal transition 15 bioRxiv preprint doi: https://doi.org/10.1101/519611; this version posted January 13, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity.
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Loss of E-Cadherin-Dependent Cell–Cell Adhesion and the Development and Progression of Cancer
Downloaded from http://cshperspectives.cshlp.org/ on October 3, 2021 - Published by Cold Spring Harbor Laboratory Press Loss of E-Cadherin-Dependent Cell–Cell Adhesion and the Development and Progression of Cancer Heather C. Bruner1 and Patrick W.B. Derksen2 1Department of Medicine, University of California at San Diego, La Jolla, California 92093 2Department of Pathology, University Medical Center Utrecht, Utrecht 3584CX, The Netherlands Correspondence: [email protected] Classical cadherins are the key molecules that control cell–cell adhesion. Notwithstanding this function, it is also clear that classical cadherins are more than just the “glue” that keeps the cells together. Cadherins are essential regulators of tissue homeostasis that govern mul- tiple facets of cellular function and development, by transducing adhesive signals to a complex network of signaling effectors and transcriptional programs. In cancer, cadherins are often inactivated or functionally inhibited, resulting in disease development and/or progression. This review focuses on E-cadherin and its causal role in the development and progression of breast and gastric cancer. We provide a summary of the biochemical conse- quences and consider the conceptual impact of early (mutational) E-cadherin loss in cancer. We advocate that carcinomas driven by E-cadherin loss should be considered “actin-dis- eases,” caused by the specific disruption of the E-cadherin-actin connection and a subse- quent dependence on sustained actomyosin contraction for tumor progression. Based on the available data from mouse and human studies we discuss opportunities for targeted clinical intervention. INTRODUCTION two families is based on the presence of a distinct histidine-alanine-valine (HAV) sequence in the lassical cadherins aretransmembrane-span- first EC domain, which is important for cad- Cning adhesion molecules containing five herin engagement (Ozawa et al.
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