beamlines BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at ISSN 1600-5775 Pohang Light Source II Do-Heon Gu,a‡ Cheolsoo Eo,b‡ Seung-A Hwangbo,c Sung-Chul Ha,b b b b b b Received 17 December 2020 Jin Hong Kim, Hyoyun Kim, Chae-Soon Lee, In Deuk Seo, Young Duck Yun, b b b b b Accepted 22 April 2021 Woulwoo Lee, Hyeongju Choi, Jangwoo Kim, Jun Lim, Seungyu Rah, Jeong-Sun Kim,a Jie-Oh Lee,c Yeon-Gil Kimb* and Suk-Youl Parkb* Edited by R. W. Strange, University of Essex, aDepartment of Chemistry, Chonnam National University, Gwangju, Republic of Korea, bPohang Accelerator Laboratory, United Kingdom Pohang University of Science and Technology, 80 Jigokro-127-Beongil, Pohang, Nam-gu, Gyeongbuk 37673, Republic of Korea, and cInstitute of Membrane Proteins, Pohang University of Science and Technology, Pohang, Gyeongbuk 37673, ‡ These authors contributed equally to this Republic of Korea. *Correspondence e-mail:
[email protected],
[email protected] work. Keywords: beamlines; microfocus; BL-11C, a new protein crystallography beamline, is an in-vacuum undulator- macromolecular crystallography; protein based microfocus beamline used for macromolecular crystallography at the structures; serial crystallography. Pohang Accelerator Laboratory and it was made available to users in June 2017. The beamline is energy tunable in the range 5.0–20 keV to support conventional single- and multi-wavelength anomalous-dispersion experiments against a wide range of heavy metals. At the standard working energy of 12.659 keV, the monochromated beam is focused to 4.1 mm (V) Â 8.5 mm (H) full width at half- maximum at the sample position and the measured photon flux is 1.3 Â 1012 photons sÀ1.