Rieck Et Al.- Supplementary Gene List Figure 1- FINAL
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Separation of GFP+ and GFP- populations from Mip-GFP mice. (A) SSC (side scatter) is proportional to cell complexity and depends on the number of organelles inside the cell. FSC (forward scatter) correlates with cell size. GFP 530/30-A measures strength of an individual cells GFP intensity. (A-D) Freshly isolated islets from CD1+ female mouse (no GFP expression) were used to calibrate the cell sorter before GFP sorting began. (E) Plotting both SSC and FSC shows all cells collected as shown by the highlighted gate. (F) Only singlet or non-aggregated cells were used for ecient cell sorting. (G-H) Two distinct populations of cells were collected separately for further analysis. Dierentially expressed genes during pregnancy day 14.5 (FC 1.5 and FDR 20%) GeneSymbol Fold Change GeneName C85627 53.11 expressed sequence C85627 Cldn8 51.06 claudin 8 LOC382109 27.98 Hypothetical protein E330023O18 Lrrc55 26.70 leucine rich repeat containing 55 Fbxw14 25.85 F-box and WD-40 domain protein 14 Tph1 19.14 tryptophan hydroxylase 1 Itk 18.16 IL2-inducible T-cell kinase Gdf3 17.91 growth differentiation factor 3 Tph2 12.09 tryptophan hydroxylase 2 Cish 10.88 cytokine inducible SH2-containing protein 8.91 EG382156 8.77 predicted gene, EG382156 1700124P09Rik 8.70 RIKEN cDNA 1700124P09 gene Gpc6 7.29 glypican 6 5830443L24Rik 7.25 RIKEN cDNA 5830443L24 gene Fmo1 7.20 flavin containing monooxygenase 1 5830417I10Rik 7.09 RIKEN cDNA 5830417I10 gene Sftpd 6.98 surfactant associated protein D 6430550H21Rik 6.39 RIKEN cDNA 6430550H21 gene 1810009J06Rik 6.38 RIKEN cDNA 1810009J06 gene Neb 5.84 nebulin Ehhadh 5.71 enoyl-Coenzyme A LOC382106 5.65 hypothetical protein LOC382106 9430069I07Rik 5.58 RIKEN cDNA 9430069I07 gene Crispld2 5.28cysteine-rich secretory protein LCCL domain containing 2 Il1rn 5.14 interleukin 1 receptor antagonist 5.10 ENSMUST00000028318 Pax8 4.87 paired box gene 8 Oas2 4.80 2'-5' oligoadenylate synthetase 2 Rasgrp1 4.77 RAS guanyl releasing protein 1 4933425B16Rik 4.70 RIKEN cDNA 4933425B16 gene Igfbp5 4.40 insulin-like growth factor binding protein 5 Lztr2 4.38 leucine zipper transcription regulator 2 E330037M01Rik 4.31 RIKEN cDNA E330037M01 gene B3galnt1 UDP-GalNAc:betaGlcNAc4.28 beta 1,3-galactosaminyltransferase, polypeptide 1 Fbxw15 4.24 F-box and WD-40 domain protein 15 Chgb 4.19 chromogranin B Dnmt3b 4.19 DNA methyltransferase 3B D5Ertd579e 4.18 DNA segment, Chr 5, ERATO Doi 579, expressed Cspg5 4.15 chondroitin sulfate proteoglycan 5 Apobec1 3.98 apolipoprotein B editing complex 1 Lonrf3 3.97 LON peptidase N-terminal domain and ring finger 3 Krt80 3.91 keratin 80 Txnrd2 3.90 thioredoxin reductase 2 Dnahc8 3.88 dynein, axonemal, heavy chain 8 Matn2 3.59 matrilin 2 Gbp4 3.54 guanylate nucleotide binding protein 4 3.42 Hsbp1 3.38 heat shock factor binding protein 1 Ngfr 3.35nerve growth factor receptor (TNFR superfamily, member 16) Ivd 3.34 isovaleryl coenzyme A dehydrogenase 3.32 Olfr58 3.28 olfactory receptor 58 Trib3 3.27 tribbles homolog 3 (Drosophila) Tnfrsf11b 3.27tumor necrosis factor receptor superfamily, member 11b 3.26 Grem2 3.24gremlin 2 homolog, cysteine knot superfamily (Xenopus laevis) 3.21 3.20 3.19 BC019537 3.19 cDNA sequence BC019537 C030047K22Rik 3.17 RIKEN cDNA C030047K22 gene Ipo11 3.17 importin 11 Angptl6 3.14 angiopoietin-like 6 3.10 Socs2 3.08 suppressor of cytokine signaling 2 Olfr330 3.05 olfactory receptor 330 Slc2a13 solute3.05 carrier family 2 (facilitated glucose transporter), member 13 Hod 3.04 homeobox only domain 4833427G06Rik 3.03 RIKEN cDNA 4833427G06 gene 2900024C23Rik 3.01 RIKEN cDNA 2900024C23 gene Mup1 2.99 major urinary protein 1 2810055F11Rik 2.99 RIKEN cDNA 2810055F11 gene 1810044A24Rik 2.96 RIKEN cDNA 1810044A24 gene 4930527E24Rik 2.93 RIKEN cDNA 4930527E24 gene Pycr1 2.92 pyrroline-5-carboxylate reductase 1 Acta2 2.91 actin, alpha 2, smooth muscle, aorta Otoa 2.91 otoancorin Slc40a1 2.88solute carrier family 40 (iron-regulated transporter), member 1 A230106N23Rik 2.88 RIKEN cDNA A230106N23 gene Car15 2.85 carbonic anhydrase 15 2.83 Mbc2 2.81 membrane bound C2 domain containing protein 4933437F05Rik 2.78 RIKEN cDNA 4933437F05 gene Kl 2.78 klotho Grhl1 2.77 grainyhead-like 1 (Drosophila) D830013H12 2.76 hypothetical protein D830013H12 BC057170 2.76 cDNA sequence BC057170 1110018M03Rik 2.75 RIKEN cDNA 1110018M03 gene BC006662 2.75 cDNA sequence BC006662 Fmo4 2.75 flavin containing monooxygenase 4 4930488P18Rik 2.74 RIKEN cDNA 4930488P18 gene Ugt1a6b 2.74UDP glucuronosyltransferase 1 family, polypeptide A6B Tbc1d4 2.73 TBC1 domain family, member 4 Kirrel3 2.73 kin of IRRE like 3 (Drosophila) Bmp1 2.73 bone morphogenetic protein 1 Birc5 2.73 baculoviral IAP repeat-containing 5 Epb4.1l3 2.72 erythrocyte protein band 4.1-like 3 4930502E18Rik 2.70 RIKEN cDNA 4930502E18 gene Aqp4 2.70 aquaporin 4 2.69 B3galt1 UDP-Gal:betaGlcNAc2.66 beta 1,3-galactosyltransferase, polypeptide 1 Wipi1 2.66 WD repeat domain, phosphoinositide interacting 1 B630006K09Rik 2.65 RIKEN cDNA B630006K09 gene 5430401F13Rik 2.64 RIKEN cDNA 5430401F13 gene V1rc32 2.62 vomeronasal 1 receptor, C32 Prlr 2.62 prolactin receptor Myo3a 2.61 myosin IIIA C77713 2.61 expressed sequence C77713 Map3k13 2.60 mitogen-activated protein kinase kinase kinase 13 Hemk1 2.59 HemK methyltransferase family member 1 Cnn1 2.59 calponin 1 2.58 Clec1b 2.58 C-type lectin domain family 1, member b Derl3 2.57 Der1-like domain family, member 3 Zmynd10 2.57 zinc finger, MYND domain containing 10 Galt 2.57 galactose-1-phosphate uridyl transferase Neil3 2.57 nei like 3 (E. coli) Palld 2.56 palladin, cytoskeletal associated protein 2.56 Rbp7 2.56 retinol binding protein 7, cellular Aqp3 2.55 aquaporin 3 MGC118210 2.55 similar to XMR Gpr83 2.54 G protein-coupled receptor 83 Atf5 2.53 activating transcription factor 5 C3 2.53 complement component 3 C6 2.53 complement component 6 9430071P14Rik 2.52 RIKEN cDNA 9430071P14 gene Rbmx2 2.52 RNA binding motif protein, X-linked 2 Syce2 2.51 synaptonemal complex central element protein 2 2610209C05Rik 2.51 RIKEN cDNA 2610209C05 gene Serinc5 2.51 serine incorporator 5 Igfals 2.50insulin-like growth factor binding protein, acid labile subunit Enpp2 2.50ectonucleotide pyrophosphatase/phosphodiesterase 2 Lsm6 LSM62.48 homolog, U6 small nuclear RNA associated (S. cerevisiae) 2.48 2.48 E330009P21Rik 2.47 RIKEN cDNA E330009P21 gene Cpxm2 2.47 carboxypeptidase X 2 (M14 family) Abhd2 2.46 abhydrolase domain containing 2 Acvr1c 2.46 activin A receptor, type IC Lrrc8c 2.44 leucine rich repeat containing 8 family, member C Chac1 2.43 ChaC, cation transport regulator-like 1 (E. coli) Aass 2.42 aminoadipate-semialdehyde synthase Cldn2 2.42 claudin 2 Has2 2.41 hyaluronan synthase 2 Emilin1 2.40 elastin microfibril interfacer 1 Hapln4 2.40 hyaluronan and proteoglycan link protein 4 Dct 2.40 dopachrome tautomerase Mrpplf4 2.40 mitogen regulated protein, proliferin 4 C730048C13Rik 2.39 RIKEN cDNA C730048C13 gene Rims3 2.38 regulating synaptic membrane exocytosis 3 Il18bp 2.38 interleukin 18 binding protein Il18bp 2.36 interleukin 18 binding protein EG330097 2.36 predicted gene, EG330097 Creg1 2.35 cellular repressor of E1A-stimulated genes 1 V1rc14 2.35 vomeronasal 1 receptor, C14 Angptl7 2.34 angiopoietin-like 7 Olfr25 2.34 olfactory receptor 25 Tubb6 2.32 tubulin, beta 6 Slc2a6 solute2.32 carrier family 2 (facilitated glucose transporter), member 6 Creb3l3 2.32 cAMP responsive element binding protein 3-like 3 Gas5 2.31 growth arrest specific 5 Gja5 2.31 gap junction membrane channel protein alpha 5 Prkcm 2.30 protein kinase C, mu Fgl2 2.30 fibrinogen-like protein 2 Ott 2.28 ovary testis transcribed Nupr1 2.27 nuclear protein 1 2.27 Wnt9a 2.27 wingless-type MMTV integration site 9A 2.27 Lgals12 2.25 lectin, galactose binding, soluble 12 Wdr63 2.25 WD repeat domain 63 LOC638895 2.25 similar to vomeronasal 2, receptor, 1 Bnip3 2.25 BCL2/adenovirus E1B interacting protein 1, NIP3 Gna14 2.23 guanine nucleotide binding protein, alpha 14 Hecw2 HECT,2.23 C2 and WW domain containing E3 ubiquitin protein ligase 2 Phgdh 2.23 3-phosphoglycerate dehydrogenase Dpysl3 2.23 dihydropyrimidinase-like 3 Gabrp 2.23 gamma-aminobutyric acid (GABA-A) receptor, pi D2Ertd750e 2.23 DNA segment, Chr 2, ERATO Doi 750, expressed Mocos 2.22 molybdenum cofactor sulfurase LOC674214 2.22 hypothetical protein LOC674214 Sh3gl3 2.20 SH3-domain GRB2-like 3 Adamts9a disintegrin-like2.20 and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 9 Dppa3 2.20 developmental pluripotency-associated 3 Isg20 2.19 interferon-stimulated protein Id4 2.19 inhibitor of DNA binding 4 AI132189 2.17 expressed sequence AI132189 Npcd 2.17 neuronal pentraxin with chromo domain Aicda 2.17 activation-induced cytidine deaminase Lsm6 LSM62.16 homolog, U6 small nuclear RNA associated (S. cerevisiae) Olfr987 2.16 olfactory receptor 987 Pdlim4 2.16 PDZ and LIM domain 4 4933409K07Rik 2.16 RIKEN cDNA 4933409K07 gene B830017H08Rik 2.16 RIKEN cDNA B830017H08 gene 4933405D12Rik 2.16 RIKEN cDNA 4933405D12 gene Ovol2 2.16 ovo-like 2 (Drosophila) B930046C15Rik 2.15 RIKEN cDNA B930046C15 gene Gm1077 2.15 gene model 1077, (NCBI) Gm459 2.15 gene model 459, (NCBI) Oprs1 2.15 opioid receptor, sigma 1 2810026P18Rik 2.15 RIKEN cDNA 2810026P18 gene 2.14 Fhad1 2.13forkhead-associated (FHA) phosphopeptide binding domain 1 C8g 2.13 complement component 8, gamma subunit Ikzf4 2.12 IKAROS family zinc finger 4 LOC664837 2.12 similar to RIKEN cDNA 5031410I06 2.12 Tmem40 2.11 transmembrane protein 40 AU040320 2.11 expressed sequence AU040320 Cars 2.11 cysteinyl-tRNA synthetase D17H6S56E-5 2.11 DNA