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Piper Lineatum Ruiz & Pav. Essential Nat. Volatiles & Essent. Oils, 2020; 7(4): 14-25 Valarezo et al. DOI: 10.37929/nveo.782412 RESEARCH ARTICLE Bioactivity evaluation of the native Amazonian species of Ecuador: Piper lineatum Ruiz & Pav. essential oil Eduardo Valarezo1,*, Gabriela Merino1, Claudia Cruz-Erazo1 and Luis Cartuche1 1Departamento de Química y Ciencias Exactas, Universidad Técnica Particular de Loja, Loja 110150, ECUADOR *Corresponding author. Email: [email protected] Submitted: 18.08.2020; Accepted: 01.01.2020 Abstract In the present research, the essential oil from Piper lineatum Ruiz & Pav. was analysed by GC/MS and GC/FID, respectively. A total of thirty-seven chemical compounds were identified, which represented 98.9% of the essential oil composition. The main compounds were apiole (21.5%), safrole (19.2%), and myristicin (13.8%), respectively. The in vitro antimicrobial activity and antifungal activity of the oil was assayed against two Gram positive bacteria, five Gram negative bacteria and two fungi. The essential oil from P. lineatum showed an inhibitory activity against Gram-positive bacterium Klebsiella pneumoniae (ATCC 9997), and against dermatophytic fungus Trichophyton rubrum (ATCC 28188) with a MIC of 500 μg/mL in both cases. The antioxidant activity of essential oil was explored using DPPH and ABTS radical scavenging method, by means of both assays the essential oil showed a weak antioxidant activity. To the best of our knowledge, this is the first report on the chemical composition and biological activity of essential oil from this species. Keywords: Essential oil, Piper lineatum, Apiole, Safrole, Antimicrobial activity, Antioxidant activity Introduction The Piperaceae is a large family of flowering plants widely distributed throughout the tropical and subtropical regions (Yuncker, 1973). The Piperaceae also known as the pepper family belongs to order Piperales. The family comprises about 13 genera (The Plant List, 2013), of which the genera Piper and Peperomia contain more than 98% of the species (about 2618 species), and the other three genera contain less than 2% (about 40 species) (Missouri Botanical Garden, 2017). The genus Piper is the largest in the Piperaceae family and is made up of around 1457 species (Missouri Botanical Garden, 2017), many of which have been used as culinary spices and recognized by their medicinal properties in traditional medicine background. Worldwide Piper species are widely used for their antibacterial, antifungal, anti-inflammatory and disinfectant effects and to prevent pains, stomach-ache, and as remedies against parasites, fever, gastritis, flu, rheumatism, cough, headache, skin, prostate problems, etc. (Salehi et al., 2019). In South America, a large number of species of this genus have been reported with uses in traditional medicine (Salehi et al., 2019). As an example, Piper umbellatum L. is traditionally used as an anti-inflammatory in Brazil and to treat fever in Peru (Duke, Bogenschutz-Godwin, & Ottesen, 2009). Piper marginatum Jacq. leaves and stems are used in Brazil, especially in Paraíba State, against snake bites and as a sedative, while in north-eastern Brazil and in the northern region, especially in the Amazon, P. marginatum is commonly used for the treatment of inflammatory diseases, snake bites, as well as the liver and bile duct diseases (Brú & Guzman, 2016). Durant-Archibold et al. have reported on the folklore uses of P. hispidum in various South American countries, a leaf infusion is drunk for its antihemorrhagic and diuretic effects in Brazil while in Ecuador it is applied to kill head lice and a leaf decoction is used in Colombia to treat malaria (Durant-Archibold, Santana, & Gupta, 2018). Piper genus is a well-known and widely distributed pantropical taxon of aromatic plants, rich in essential oils (EOs), which can be found in many tissues and organs: fruits, seeds, leaves, branches, roots and stems (da Silva et al., 2017; Naim & Mahboob, 2020). Piper essential oils have insecticidal, acaricidal, repellent and antifeedant effects and some are nematicidal and phytotoxic (Jaramillo-Colorado, Pino-Benitez, & González- 14 Nat. Volatiles & Essent. Oils, 2020; 7(4): 14-25 Valarezo et al. DOI: 10.37929/nveo.782412 Coloma, 2019). Piper species EOs are rich in all classes of volatile chemical compounds, but the composition is highly variable and these differences seem to depend on polymorphism, plant part, geographical differences, environmental conditions and chemotypes (Thin et al., 2018). According to Mgbeahuruike, Yrjönen, Vuorela and Holm (2017) there are more than 270 identified compounds in Piper spp. EOs. Amongst the most important compounds found in the Piper EOs are monoterpene hydrocarbons, oxygenated monoterpenoids, sesquiterpene hydrocarbons and oxygenated sesquiterpenoids, also in these oils you can find the phenylpropanoids such as safrole, dillapiole, myristicin, elemicin, (Z)-asarone, eugenol, apiole, and sarisan (da Silva et al., 2017). Safrole is abundant in nature among diverse plant genera such as Sassafras, Ocotea, Cinnamomum, Myristica, and Piper (Kemprai et al., 2020), in EOs of some species like Piper hispidinervum collected in southern Brazil this compound is even found in percentages of around 80% (Andrés et al., 2017). Safrol is recognized as a weak hepatocarcinogen with demonstrated genotoxicity in rodents led to strict restrictions on its use in food by various regulatory bodies globally. Which has caused that many government authorities have enforced laws to restrict the production and harvesting of safrole‐ bearing plants (Kemprai et al., 2020). However, the essential oils that contain safrol show biological activity, especially repellent activity, for this reason some scientists suggest the use of these oils as a potential natural insecticide, for example for stored-product insect pests (Zapata & Smagghe, 2010). For Ecuador, 450 species of the Piperaceae family belonging to four genera have been registered, of which the most diverse is the genus Peperomia with 180 native and 50 endemic species, followed by the genus Piper with 157 native and 61 endemic species (Jørgesen & León-Yáñez, 1999; León-Yánez et al., 2019). Ecuadorian native communities in the Amazonian region use different species of Piper. For example, the stems of P. augustum and P. conejosense are broken off and used as toothbrushes (Davis & Yost, 1983; Evans Schultes, 1985). Piper ecuadorense, known as matico de monte, is a plant used by many indigenous communities in Loja and Zamora provinces to treat hangover, as a disinfectant and in wound healing (Ramírez, Cartuche, Morocho, Aguilar, & Malagon, 2013). The Leaf and flower of Piper aduncum L. are used as disinfectant, gastritis, influenza, rheumatism and cough. The whole plant of Piper barbatum Kunth, named as Cordoncillo is used to treat headache, stomach pain, dermatitis, disinfectant, the leaf of Piper bogotense C. DC. called Sacha guando is used in hepatic pain, the stem and leaf of Piper crassinervium Kunth (Guabiduca) is used to treat diabetes, gastritis, prostate problems and the leaf of Piper ecuadorense Sodiro (matico del monte) is used as hangover, disinfectant, healing of wounds (Tene et al., 2007). Piper lineatum Ruiz & Pav. is a native aromatic shrub of Ecuador and Peru. In Ecuador is widely distributed between 0-2500 m a.s.l in the Amazonian provinces of Morona Santiago, Napo, Pastaza, Pichincha, Tungurahua and Zamora Chinchipe, and in the Andean province of Pichincha (Tropicos, 2020). Ecuador is considered a biodiversity hotspot with many vegetal species per unit surface area, which locates this country in the sixth position in the world. Piper aromatic species in Ecuador have been used as a condiment or in traditional medicine, for that reason, the aim of this research was to determine the chemical composition and antifungal and antibacterial activity of the essential oil of Piper lineatum, as well as, to value its antioxidant activity. Materials and Methods Materials Dichloromethane, sodium sulfate anhydrous, 2,2-diphenyl-1-picrylhydryl (DPPH•) and 2,2’-azinobis-3- ethylbenzothiazoline-6-sulfonic acid (ABTS) were purchased from Sigma-Aldrich. Alliphatic hydrocarbons standard was purchased from CHEM SERVICE under the name of Diesel Range Organics Mixture #2-GRO/DRO 15 Nat. Volatiles & Essent. Oils, 2020; 7(4): 14-25 Valarezo et al. DOI: 10.37929/nveo.782412 and with the code M-TPH6X4-1ML. Helium was purchased from INDURA Ecuador. Microbiological media as Sabourad and Mueller Himton Broth were purchased from DIPCO. Dimethylsulfoxide (DMSO) was obtained from Fisher. All chemicals were of analytical grade and used without further purifications. Plant material The collection of the species, authorized by the Ministerio del Ambiente de Ecuador (MAE), with authorization N° 001-IC-FLO-DBAP-VS-DRLZCH-MA, was performed in Zamora canton, province of Zamora Chinchipe, Ecuadorian Amazon, at a latitude of 4°08'25" S and a longitude of 78°55'51" W. The collection was performed at an altitude of 1075 m a.s.l. The storage and transfer of the plant material was carried out in airtight plastic containers until they were hydrodistilled. Temperature collection was at room temperature (26°C) and the transfer temperature was 24 °C, the pressure was approximately 88 KPa (room pressure). The botanical specimens were identified by Dr. Bolivar Merino, at the herbarium of the “Universidad Nacional de Loja”. A voucher specimen is preserved in the Herbarium of the Universidad Técnica Particular de Loja under code PPN-809. Moisture of plant
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