10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609

ARRB2 / Beta 2 Antibody (aa23-40) Rabbit Polyclonal Antibody Catalog # ALS15263

Specification

ARRB2 / Beta Arrestin 2 Antibody (aa23-40) - Product Information

Application WB, IHC Primary Accession P32121 Reactivity Human, Mouse, Rat, Rabbit, Monkey, Pig, Horse, Bovine, Dog Host Rabbit Clonality Polyclonal Calculated MW 46kDa KDa

ARRB2 / Beta Arrestin 2 Antibody (aa23-40) - Additional Information Western blot of ARRB2 in HUVEC cell lysate in the 1) absence and 2) presence of immunizing... ID 409

Other Names Beta-arrestin-2, Arrestin beta-2, ARRB2, ARB2, ARR2

Target/Specificity Human ARRB2 / Beta-Arrestin 2

Reconstitution & Storage Store at 4°C for short term applications. For long term storage, aliquot and store at -20°C.

Precautions Anti-ARRB2 antibody IHC of human thyroid. ARRB2 / Beta Arrestin 2 Antibody (aa23-40) is for research use only and not for use in diagnostic or therapeutic procedures. ARRB2 / Beta Arrestin 2 Antibody (aa23-40) - Background

ARRB2 / Beta Arrestin 2 Antibody (aa23-40) - Functions in regulating agonist-mediated Information G-protein coupled (GPCR) signaling by mediating both receptor desensitization and Name ARRB2 resensitization processes. During homologous desensitization, beta- bind to the Synonyms ARB2, ARR2 GPRK-phosphorylated receptor and sterically preclude its coupling to the cognate G- protein; Function the binding appears to require additional Functions in regulating agonist-mediated receptor determinants exposed only in the G-protein coupled receptor (GPCR) signaling active receptor conformation. The

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by mediating both receptor desensitization beta-arrestins target many receptors for and resensitization processes. During internalization by acting as endocytic adapters homologous desensitization, beta- arrestins (CLASPs, clathrin-associated sorting ) bind to the GPRK-phosphorylated receptor and recruiting the GPRCs to the adapter and sterically preclude its coupling to the protein 2 complex 2 (AP-2) in clathrin-coated cognate G-protein; the binding appears to pits (CCPs). However, the extent of require additional receptor determinants beta-arrestin involvement appears to vary exposed only in the active receptor significantly depending on the receptor, conformation. The beta-arrestins target agonist and cell type. Internalized many receptors for internalization by acting arrestin-receptor complexes traffic to as endocytic adapters (CLASPs, clathrin- intracellular endosomes, where they remain associated sorting proteins) and recruiting uncoupled from G-proteins. Two different the GPRCs to the adapter protein 2 complex modes of arrestin-mediated internalization 2 (AP-2) in clathrin-coated pits (CCPs). occur. Class A receptors, like ADRB2, OPRM1, However, the extent of beta-arrestin involvement appears to vary significantly ENDRA, D1AR and ADRA1B dissociate from depending on the receptor, agonist and cell beta- arrestin at or near the plasma membrane type. Internalized arrestin-receptor and undergo rapid recycling. Class B receptors, complexes traffic to intracellular like AVPR2, AGTR1, NTSR1, TRHR and TACR1 endosomes, where they remain uncoupled internalize as a complex with arrestin and from G-proteins. Two different modes of traffic with it to endosomal vesicles, arrestin- mediated internalization occur. presumably as desensitized receptors, for Class A receptors, like ADRB2, OPRM1, extended periods of time. Receptor ENDRA, D1AR and ADRA1B dissociate from resensitization then requires that beta-arrestin at or near the plasma receptor-bound arrestin is removed so that the membrane and undergo rapid recycling. receptor can be dephosphorylated and Class B receptors, like AVPR2, AGTR1, returned to the plasma membrane. Mediates NTSR1, TRHR and TACR1 internalize as a endocytosis of CCR7 following ligation of complex with arrestin and traffic with it to CCL19 but not CCL21. Involved in endosomal vesicles, presumably as internalization of P2RY1, P2RY4, P2RY6 and desensitized receptors, for extended P2RY11 and ATP-stimulated internalization of periods of time. Receptor resensitization P2RY2. Involved in phosphorylation-dependent then requires that receptor-bound arrestin internalization of OPRD1 and subsequent is removed so that the receptor can be recycling or degradation. Involved in dephosphorylated and returned to the ubiquitination of IGF1R. Beta-arrestins function plasma membrane. Mediates endocytosis of as multivalent adapter proteins that can switch CCR7 following ligation of CCL19 but not the GPCR from a G-protein signaling mode that CCL21. Involved in internalization of P2RY1, transmits short-lived signals from the plasma P2RY4, P2RY6 and P2RY11 and membrane via small molecule second ATP-stimulated internalization of P2RY2. Involved in phosphorylation-dependent messengers and ion channels to a internalization of OPRD1 and subsequent beta-arrestin signaling mode that transmits a recycling or degradation. Involved in distinct set of signals that are initiated as the ubiquitination of IGF1R. Beta- arrestins receptor internalizes and transits the function as multivalent adapter proteins intracellular compartment. Acts as signaling that can switch the GPCR from a G-protein scaffold for MAPK pathways such as MAPK1/3 signaling mode that transmits short-lived (ERK1/2) and MAPK10 (JNK3). ERK1/2 and JNK3 signals from the plasma membrane via activated by the beta-arrestin scaffold are small molecule second messengers and ion largely excluded from the nucleus and channels to a beta-arrestin signaling mode confined to cytoplasmic locations such as that transmits a distinct set of signals that endocytic vesicles, also called beta-arrestin are initiated as the receptor internalizes signalosomes. Acts as signaling scaffold for the and transits the intracellular compartment. AKT1 pathway. GPCRs for which the Acts as signaling scaffold for MAPK beta-arrestin-mediated signaling relies on both pathways such as MAPK1/3 (ERK1/2) and ARRB1 and ARRB2 (codependent regulation) MAPK10 (JNK3). ERK1/2 and JNK3 activated include ADRB2, F2RL1 and PTH1R. For some by the beta-arrestin scaffold are largely GPCRs the beta-arrestin- mediated signaling

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excluded from the nucleus and confined to relies on either ARRB1 or ARRB2 and is cytoplasmic locations such as endocytic inhibited by the other respective beta-arrestin vesicles, also called beta-arrestin form (reciprocal regulation). Increases ERK1/2 signalosomes. Acts as signaling scaffold for signaling in AGTR1- and AVPR2- mediated the AKT1 pathway. GPCRs for which the activation (reciprocal regulation). Involved in beta-arrestin- mediated signaling relies on CCR7- mediated ERK1/2 signaling involving both ARRB1 and ARRB2 (codependent CCL19. Is involved in type-1A regulation) include ADRB2, F2RL1 and angiotensin II receptor/AGTR1-mediated ERK PTH1R. For some GPCRs the beta- activity. Is involved in type-1A angiotensin II arrestin-mediated signaling relies on either receptor/AGTR1-mediated MAPK10 activity. Is ARRB1 or ARRB2 and is inhibited by the involved in dopamine-stimulated AKT1 activity other respective beta-arrestin form in the striatum by disrupting the association of (reciprocal regulation). Increases ERK1/2 AKT1 with its negative regulator PP2A. Involved signaling in AGTR1- and AVPR2-mediated in AGTR1-mediated chemotaxis. Appears to activation (reciprocal regulation). Involved in CCR7-mediated ERK1/2 signaling function as signaling scaffold involved in involving ligand CCL19. Is involved in regulation of MIP-1- beta-stimulated type-1A angiotensin II CCR5-dependent chemotaxis. Involved in receptor/AGTR1-mediated ERK activity. Is attenuation of NF-kappa-B-dependent involved in type-1A angiotensin II transcription in response to GPCR or cytokine receptor/AGTR1-mediated MAPK10 activity. stimulation by interacting with and stabilizing Is involved in dopamine-stimulated AKT1 CHUK. Suppresses UV-induced activity in the striatum by disrupting the NF-kappa-B-dependent activation by association of AKT1 with its negative interacting with CHUK. The function is regulator PP2A. Involved in promoted by stimulation of ADRB2 and AGTR1-mediated chemotaxis. Appears to dephosphorylation of ARRB2. Involved in function as signaling scaffold involved in p53/TP53- mediated apoptosis by regulating regulation of MIP-1-beta-stimulated and reducing the MDM2- mediated CCR5-dependent chemotaxis. Involved in degradation of p53/TP53. May serve as nuclear attenuation of NF-kappa-B-dependent messenger for GPCRs. Upon stimulation of transcription in response to GPCR or OR1D2, may be involved in regulation of gene cytokine stimulation by interacting with and expression during the early processes of stabilizing CHUK. Suppresses UV-induced fertilization. Also involved in regulation of NF-kappa- B-dependent activation by receptors other than GPCRs. Involved in interacting with CHUK. The function is endocytosis of TGFBR2 and TGFBR3 and down- promoted by stimulation of ADRB2 and regulates TGF-beta signaling such as dephosphorylation of ARRB2. Involved in NF-kappa-B activation. Involved in endocytosis p53/TP53-mediated apoptosis by regulating of low-density lipoprotein receptor/LDLR. MDM2 and reducing the MDM2-mediated Involved in endocytosis of degradation of p53/TP53. May serve as nuclear messenger for GPCRs. Upon homolog/Smo, which also requires ADRBK1. stimulation of OR1D2, may be involved in Involved in endocytosis of SLC9A5. Involved in regulation of during the endocytosis of ENG and subsequent early processes of fertilization. Also TGF-beta-mediated ERK activation and involved in regulation of receptors other migration of epithelial cells. Involved in than GPCRs. Involved in endocytosis of Toll-like receptor and IL-1 receptor signaling TGFBR2 and TGFBR3 and down-regulates through the interaction with TRAF6 which TGF-beta signaling such as NF-kappa-B prevents TRAF6 autoubiquitination and activation. Involved in endocytosis of oligomerization required for activation of low-density lipoprotein receptor/LDLR. NF-kappa-B and JUN. Involved in insulin Involved in endocytosis of smoothened resistance by acting as insulin-induced homolog/Smo, which also requires GRK2. signaling scaffold for SRC, AKT1 and INSR. Involved in endocytosis of SLC9A5. Involved Involved in regulation of inhibitory signaling of in endocytosis of ENG and subsequent natural killer cells by recruiting PTPN6 and TGF-beta-mediated ERK activation and PTPN11 to KIR2DL1. Involved in IL8-mediated migration of epithelial cells. Involved in granule release in neutrophils. Involved in the Toll-like receptor and IL-1 receptor signaling internalization of the atypical chemokine

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through the interaction with TRAF6 which receptor ACKR3. prevents TRAF6 autoubiquitination and oligomerization required for activation of ARRB2 / Beta Arrestin 2 Antibody NF- kappa-B and JUN. Involved in insulin (aa23-40) - References resistance by acting as insulin- induced signaling scaffold for SRC, AKT1 and INSR. Rapoport B.,et al.Mol. Cell. Endocrinol. Involved in regulation of inhibitory signaling 84:R39-R43(1992). of natural killer cells by recruiting PTPN6 Yu Q.M.,et al.Submitted (NOV-1998) to the and PTPN11 to KIR2DL1. Involved in EMBL/GenBank/DDBJ databases. IL8-mediated granule release in neutrophils. Sanchez-Laorden B.L.,et al.Submitted Involved in the internalization of the (MAY-2006) to the EMBL/GenBank/DDBJ atypical ACKR3. Acts as databases. an adapter protein coupling FFAR4 receptor Kaighin V.A.,et al.Submitted (JUL-2008) to the to specific downstream signaling pathways, EMBL/GenBank/DDBJ databases. as well as mediating receptor endocytosis Ota T.,et al.Nat. Genet. 36:40-45(2004). (PubMed:22282525, PubMed:23809162). During the activation step of NLRP3 inflammasome, directly associates with NLRP3 leading to inhibition of proinflammatory cytokine release and inhibition of inflammation (PubMed:23809162).

Cellular Location Cytoplasm. Nucleus. Cell membrane. Membrane, clathrin-coated pit. Cytoplasmic vesicle Note=Translocates to the plasma membrane and colocalizes with antagonist-stimulated GPCRs

ARRB2 / Beta Arrestin 2 Antibody (aa23-40) - Protocols

Provided below are standard protocols that you may find useful for product applications.

• Western Blot • Blocking Peptides • Dot Blot • Immunohistochemistry • Immunofluorescence • Immunoprecipitation • Flow Cytomety • Cell Culture

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