P2RY11 Rabbit Pab

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Leader in Biomolecular Solutions for Life Science P2RY11 Rabbit pAb Catalog No.: A16940 Basic Information Background Catalog No. The product of this gene belongs to the family of G-protein coupled receptors. This A16940 family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is Observed MW coupled to the stimulation of the phosphoinositide and adenylyl cyclase pathways and 45kDa behaves as a selective purinoceptor. Naturally occuring read-through transcripts, resulting from intergenic splicing between this gene and an immediately upstream gene Calculated MW (PPAN, encoding peter pan homolog), have been found. The PPAN-P2RY11 read- 40kDa through mRNA is ubiquitously expressed and encodes a fusion protein that shares identity with each individual gene product. Category Primary antibody Applications WB Cross-Reactivity Human, Mouse, Rat Recommended Dilutions Immunogen Information WB 1:500 - 1:2000 Gene ID Swiss Prot 5032 Q96G91 Immunogen A synthetic peptide of human P2RY11. Synonyms P2RY11;P2Y11 Contact Product Information www.abclonal.com Source Isotype Purification Rabbit IgG Affinity purification Storage Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide,50% glycerol,pH7.3. Validation Data Western blot analysis of extracts of various cell lines, using P2RY11 Rabbit pAb (A16940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s. Antibody | Protein | ELISA Kits | Enzyme | NGS | Service For research use only. Not for therapeutic or diagnostic purposes. Please visit http://abclonal.com for a complete listing of recommended products..

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Activation of Hypermethylated P2RY1 Mitigates Gastric Cancer by Promoting Apoptosis and Inhibiting Proliferation
Activation of hypermethylated P2RY1 mitigates gastric cancer by promoting apoptosis and inhibiting proliferation Yinggang Hua Xiamen University Medical College Long Li Xiamen University Medical College Liangliang Cai Zhongshan Hospital Xiamen University Guoyan Liu ( [email protected] ) Zhongshan Hospital Xiamen University Research Article Keywords: Diffuse type gastric cancer, DNA methylation 450K array, P2RY1 receptor, ERK signal pathway, Tumor suppressor gene Posted Date: July 26th, 2021 DOI: https://doi.org/10.21203/rs.3.rs-351723/v1 License: This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License Page 1/16 Abstract P2RY1 receptor is known to cause cancer by activating the ERK signal pathway, its DNA methylation status or even the corresponding regulatory mechanism remains unknown. In this study, DNA methylation chip was used to prole the genome-wide DNA methylation level in gastric cancer tissues. Proliferation and apoptosis of the SGC7901 gastric cancer cell line were determined after treatment with a selective P2RY1 receptor agonist, MRS2365. The promoter region of P2RY1 was found to be highly methylated with 4 hypermethylated sites (|Δβ value| >0.2) in diffuse gastric cancer and then were validated by bioinformatic analysis in TCGA database. Analysis of MRS2365-treated cells by annexin-V/PI staining and Caspase-3 activity assays indicated the induction of apoptosis in SGC7901 cells. P2RY1 receptor activation in human SGC7901 gastric cancer cells via the MRS2365 agonist induced apoptosis and reduced cell growth. High DNA methylation in the promoter region of P2RY1 may have contributed to the reduced expression of P2RY1’s mRNA, which is likely responsible for the “aggressive” nature of the diffuse type gastric cancer.
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G Protein-Coupled Receptors
S.P.H. Alexander et al. The Concise Guide to PHARMACOLOGY 2015/16: G protein-coupled receptors. British Journal of Pharmacology (2015) 172, 5744–5869 THE CONCISE GUIDE TO PHARMACOLOGY 2015/16: G protein-coupled receptors Stephen PH Alexander1, Anthony P Davenport2, Eamonn Kelly3, Neil Marrion3, John A Peters4, Helen E Benson5, Elena Faccenda5, Adam J Pawson5, Joanna L Sharman5, Christopher Southan5, Jamie A Davies5 and CGTP Collaborators 1School of Biomedical Sciences, University of Nottingham Medical School, Nottingham, NG7 2UH, UK, 2Clinical Pharmacology Unit, University of Cambridge, Cambridge, CB2 0QQ, UK, 3School of Physiology and Pharmacology, University of Bristol, Bristol, BS8 1TD, UK, 4Neuroscience Division, Medical Education Institute, Ninewells Hospital and Medical School, University of Dundee, Dundee, DD1 9SY, UK, 5Centre for Integrative Physiology, University of Edinburgh, Edinburgh, EH8 9XD, UK Abstract The Concise Guide to PHARMACOLOGY 2015/16 provides concise overviews of the key properties of over 1750 human drug targets with their pharmacology, plus links to an open access knowledgebase of drug targets and their ligands (www.guidetopharmacology.org), which provides more detailed views of target and ligand properties. The full contents can be found at http://onlinelibrary.wiley.com/doi/ 10.1111/bph.13348/full. G protein-coupled receptors are one of the eight major pharmacological targets into which the Guide is divided, with the others being: ligand-gated ion channels, voltage-gated ion channels, other ion channels, nuclear hormone receptors, catalytic receptors, enzymes and transporters. These are presented with nomenclature guidance and summary information on the best available pharmacological tools, alongside key references and suggestions for further reading.
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Multi-Functionality of Proteins Involved in GPCR and G Protein Signaling: Making Sense of Structure–Function Continuum with In
Cellular and Molecular Life Sciences (2019) 76:4461–4492 https://doi.org/10.1007/s00018-019-03276-1 Cellular andMolecular Life Sciences REVIEW Multi‑functionality of proteins involved in GPCR and G protein signaling: making sense of structure–function continuum with intrinsic disorder‑based proteoforms Alexander V. Fonin1 · April L. Darling2 · Irina M. Kuznetsova1 · Konstantin K. Turoverov1,3 · Vladimir N. Uversky2,4 Received: 5 August 2019 / Revised: 5 August 2019 / Accepted: 12 August 2019 / Published online: 19 August 2019 © Springer Nature Switzerland AG 2019 Abstract GPCR–G protein signaling system recognizes a multitude of extracellular ligands and triggers a variety of intracellular signal- ing cascades in response. In humans, this system includes more than 800 various GPCRs and a large set of heterotrimeric G proteins. Complexity of this system goes far beyond a multitude of pair-wise ligand–GPCR and GPCR–G protein interactions. In fact, one GPCR can recognize more than one extracellular signal and interact with more than one G protein. Furthermore, one ligand can activate more than one GPCR, and multiple GPCRs can couple to the same G protein. This defnes an intricate multifunctionality of this important signaling system. Here, we show that the multifunctionality of GPCR–G protein system represents an illustrative example of the protein structure–function continuum, where structures of the involved proteins represent a complex mosaic of diferently folded regions (foldons, non-foldons, unfoldons, semi-foldons, and inducible foldons). The functionality of resulting highly dynamic conformational ensembles is fne-tuned by various post-translational modifcations and alternative splicing, and such ensembles can undergo dramatic changes at interaction with their specifc partners.
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Initial, Transient, and Specific Interaction Between G Protein
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Differentially Methylated Genes
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Β-Nicotinamide Adenine Dinucleotide (Β-NAD) Inhibits ATP-Dependent Inflammasome Activation in Human Monocytic Cells
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MRGPRX4 Is a Novel Bile Acid Receptor in Cholestatic Itch Huasheng Yu1,2,3, Tianjun Zhao1,2,3, Simin Liu1, Qinxue Wu4, Omar
bioRxiv preprint doi: https://doi.org/10.1101/633446; this version posted May 9, 2019. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 MRGPRX4 is a novel bile acid receptor in cholestatic itch 2 Huasheng Yu1,2,3, Tianjun Zhao1,2,3, Simin Liu1, Qinxue Wu4, Omar Johnson4, Zhaofa 3 Wu1,2, Zihao Zhuang1, Yaocheng Shi5, Renxi He1,2, Yong Yang6, Jianjun Sun7, 4 Xiaoqun Wang8, Haifeng Xu9, Zheng Zeng10, Xiaoguang Lei3,5, Wenqin Luo4*, Yulong 5 Li1,2,3* 6 7 1State Key Laboratory of Membrane Biology, Peking University School of Life 8 Sciences, Beijing 100871, China 9 2PKU-IDG/McGovern Institute for Brain Research, Beijing 100871, China 10 3Peking-Tsinghua Center for Life Sciences, Beijing 100871, China 11 4Department of Neuroscience, Perelman School of Medicine, University of 12 Pennsylvania, Philadelphia, PA 19104, USA 13 5Department of Chemical Biology, College of Chemistry and Molecular Engineering, 14 Peking University, Beijing 100871, China 15 6Department of Dermatology, Peking University First Hospital, Beijing Key Laboratory 16 of Molecular Diagnosis on Dermatoses, Beijing 100034, China 17 7Department of Neurosurgery, Peking University Third Hospital, Peking University, 18 Beijing, 100191, China 19 8State Key Laboratory of Brain and Cognitive Science, CAS Center for Excellence in 20 Brain Science and Intelligence Technology (Shanghai), Institute of Biophysics, 21 Chinese Academy of Sciences, Beijing, 100101, China 22 9Department of Liver Surgery, Peking Union Medical College Hospital, Chinese bioRxiv preprint doi: https://doi.org/10.1101/633446; this version posted May 9, 2019.
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Nucleic Acid Ligands Act As a PAM and Agonist Depending on the Intrinsic Ligand Binding State of P2RY2
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Functional Associations Among G Protein-Coupled Neurotransmitter Receptors in the Human Brain Skirmantas Janušonis
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Cells and Geneblazer® P2RY2-NFAT-Bla CHO-K1 Cells Conta
Version No.: GeneBLAzer® Validation Packet Page 1 of 5 01Sep08 Optimization of the GeneBLAzer® P2RY2 NFAT-bla CHO-K1 Cell Line GeneBLAzer® P2RY2 CHO-K1 DA Assay Kit GeneBLAzer® P2RY2 NFAT-bla CHO-K1 Cells Catalog Numbers – K1337 and K1722 Cell Line Descriptions GeneBLAzer® P2RY2 CHO-K1 DA (Division Arrested) cells and GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells contain the human purinergic receptor P2, G protein-coupled, 2 (P2RY2) receptor (Accession # BC012104) stably integrated into the CellSensor® NFAT-bla CHO-K1 cell line. CellSensor® NFAT-bla CHO-K1 (Cat No. K1534) cells contain a beta-lactamase (bla) reporter gene under control of the nuclear factor of activated T cells (NFAT) response element. Division Arrested (DA) cells are available as an Assay Kit, which includes cells and sufficient substrate to analyze 1 x 384-well plate. DA cells are irreversibly division arrested using a low-dose treatment of Mitomycin-C, and have no apparent toxicity or change in cellular signal transduction. Both GeneBLAzer® P2RY2 CHO-K1 DA cells and ® GeneBLAzer P2RY2-NFAT-bla CHO-K1 cells are functionally validated for Z’-factor and EC50 concentrations of Adenosine-5’-triphosphate (ATP); (Figure 1). In addition, GeneBLAzer® P2RY2-NFAT-bla CHO-K1 cells have been tested for assay performance under variable conditions, including DMSO concentration, cell number, stimulation time, and substrate loading time. Additional testing data using alternate stimuli are also included. Target Description The P2Y receptor family is part of a larger receptor family whose physiological effects are mediated by extracellular nucleotide di- and tri-phosphates. The P2 receptor family consists of ion-gated channel receptors (P2X) and G protein coupled receptors (P2Y).
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