Journal of Clinical Medicine Article Serum N-Glycomics Stratifies Bacteremic Patients Infected with Different Pathogens Sayantani Chatterjee 1,2 , Rebeca Kawahara 1,2, Harry C. Tjondro 1,2, David R. Shaw 3, Marni A. Nenke 4,5,6, David J. Torpy 4,5 and Morten Thaysen-Andersen 1,2,* 1 Department of Molecular Sciences, Macquarie University, Sydney, NSW 2109, Australia;
[email protected] (S.C.);
[email protected] (R.K.);
[email protected] (H.C.T.) 2 Biomolecular Discovery Research Centre, Macquarie University, Sydney, NSW 2109, Australia 3 Infectious Diseases Clinic, Royal Adelaide Hospital, Adelaide, SA 5000, Australia;
[email protected] 4 Endocrine and Metabolic Unit, Royal Adelaide Hospital, Adelaide, SA 5000, Australia;
[email protected] (M.A.N.);
[email protected] (D.J.T.) 5 School of Medicine, University of Adelaide, Adelaide, SA 5000, Australia 6 Department of Endocrinology and Diabetes, The Queen Elizabeth Hospital, Woodville South, SA 5011, Australia * Correspondence:
[email protected]; Tel.: +61-2-9850-7487; Fax: +61-2-9850-6192 Abstract: Bacteremia—i.e., the presence of pathogens in the blood stream—is associated with long- term morbidity and is a potential precursor condition to life-threatening sepsis. Timely detection of bacteremia is therefore critical to reduce patient mortality, but existing methods lack precision, speed, and sensitivity to effectively stratify bacteremic patients. Herein, we tested the potential of quantitative serum N-glycomics performed using porous graphitized carbon liquid chromatography tandem mass spectrometry to stratify bacteremic patients infected with Escherichia coli (n = 11), Citation: Chatterjee, S.; Kawahara, Staphylococcus aureus (n = 11), Pseudomonas aeruginosa (n = 5), and Streptococcus viridans (n = 5) from R.; Tjondro, H.C.; Shaw, D.R.; Nenke, healthy donors (n = 39).