CHMP5 Polyclonal Antibody

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CHMP5 polyclonal antibody Catalog # : PAB6771 規格 : [ 100 ug ] List All Specification Application Image Product Goat polyclonal antibody raised against synthetic peptide of CHMP5. Western Blot (Cell lysate) Description: Immunogen: A synthetic peptide corresponding to human CHMP5. Sequence: C-NKDGVLVDEFGLPQ enlarge Host: Goat ELISA Theoretical MW 24.5 (kDa): Reactivity: Human Form: Liquid Purification: Antigen affinity purification Concentration: 0.5 mg/mL Quality Control Antibody Reactive Against Synthetic Peptide. Testing: Recommend ELISA (1:8000) Usage: Western Blot (1-3 ug/mL) The optimal working dilution should be determined by the end user. Storage Buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) Storage Store at -20°C. Instruction: Aliquot to avoid repeated freezing and thawing. Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Datasheet: Download Publication Reference 1. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J.J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11. Applications Western Blot (Cell lysate) Page 1 of 2 2017/2/16 CHMP5 polyclonal antibody (Cat # PAB6771) (0.5 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. ELISA Gene Information Entrez GeneID: 51510 Protein NP_057494.2 Accession#: Gene Name: CHMP5 Gene Alias: C9orf83,CGI-34,HSPC177,PNAS-2,SNF7DC2 Gene chromatin modifying protein 5 Description: Omim ID: 610900 Gene Ontology: Hyperlink Gene Summary: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs). Some CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP, CHMP1A (MIM 164010), is required for both MVB formation and regulation of cell cycle progression (Tsang et al., 2006 [PubMed 16730941]).[supplied by OMIM Other OTTHUMP00000021197,SNF7 domain containing 2 Designations: Gene Pathway Endocytosis 服務條款 | 隱私權政策 | 著作及商標 | 網站地圖 ©2017 亞諾法生技股份有限公司 Abnova Corporation. 版權所有. Page 2 of 2 2017/2/16.

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CHMP5 Antibody (C-Term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap18536b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 CHMP5 Antibody (C-term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP18536b Specification CHMP5 Antibody (C-term) - Product Information Application WB,E Primary Accession Q9NZZ3 Other Accession Q4QQV8, Q9D7S9, NP_057494.3 Reactivity Human Predicted Mouse, Rat Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Antigen Region 178-204 CHMP5 Antibody (C-term) - Additional Information CHMP5 Antibody (C-term) (Cat. #AP18536b) western blot analysis in MDA-MB231 cell line Gene ID 51510 lysates (35ug/lane).This demonstrates the Other Names CHMP5 antibody detected the CHMP5 protein Charged multivesicular body protein 5, (arrow). Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, CHMP5 Antibody (C-term) - Background Vps60, hVps60, CHMP5, C9orf83, SNF7DC2 CHMP5 belongs to the chromatin-modifying Target/Specificity protein/charged This CHMP5 antibody is generated from multivesicular body protein (CHMP) family. rabbits immunized with a KLH conjugated These proteins are synthetic peptide between 178-204 amino components of ESCRT-III (endosomal sorting acids from the C-terminal region of human complex required for CHMP5. transport III), a complex involved in degradation of surface Dilution receptor proteins and formation of endocytic WB~~1:1000 multivesicular bodies (MVBs). Some CHMPs have both nuclear and Format cytoplasmic/vesicular Purified polyclonal antibody supplied in PBS distributions, and one such CHMP, CHMP1A with 0.09% (W/V) sodium azide. This (MIM 164010), is required antibody is purified through a protein A for both MVB formation and regulation of cell column, followed by peptide affinity cycle progression purification.
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32-3532: CHMP5 Recombinant Protein Description Product Info
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-3532: CHMP5 Recombinant Protein Charged Multivesicular Body Protein 5,SNF7 Domain-Containing Protein 2,Chromosome 9 Open Reading Alternative Frame 83,Chromatin-Modifying Protein 5,Apoptosis-Related Protein PNAS-2,Vacuolar Protein Sorting- Name : Associated Protein 60,Chromatin Modifying Protein Description Source : E.coli. CHMP5 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-219) and having a molecular mass of 27.0 kDa.CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Charged Multivesicular Body Protein 5 (CHMP5) is a member of the chromatin- modifying protein/charged multivesicular body protein (CHMP) family. These proteins comprise the ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and development of endocytic multivesicular bodies (MVBs). Certain CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP the CHMP1A, is essential for both MVB formation and regulation of cell cycle progression. Product Info Amount : 20 µg Purification : Greater than 80% as determined by SDS-PAGE. The CHMP5 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 30% Content : glycerol. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of Storage condition : time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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Downloaded Per Proteome Cohort Via the Web- Site Links of Table 1, Also Providing Information on the Deposited Spectral Datasets
www.nature.com/scientificreports OPEN Assessment of a complete and classifed platelet proteome from genome‑wide transcripts of human platelets and megakaryocytes covering platelet functions Jingnan Huang1,2*, Frauke Swieringa1,2,9, Fiorella A. Solari2,9, Isabella Provenzale1, Luigi Grassi3, Ilaria De Simone1, Constance C. F. M. J. Baaten1,4, Rachel Cavill5, Albert Sickmann2,6,7,9, Mattia Frontini3,8,9 & Johan W. M. Heemskerk1,9* Novel platelet and megakaryocyte transcriptome analysis allows prediction of the full or theoretical proteome of a representative human platelet. Here, we integrated the established platelet proteomes from six cohorts of healthy subjects, encompassing 5.2 k proteins, with two novel genome‑wide transcriptomes (57.8 k mRNAs). For 14.8 k protein‑coding transcripts, we assigned the proteins to 21 UniProt‑based classes, based on their preferential intracellular localization and presumed function. This classifed transcriptome‑proteome profle of platelets revealed: (i) Absence of 37.2 k genome‑ wide transcripts. (ii) High quantitative similarity of platelet and megakaryocyte transcriptomes (R = 0.75) for 14.8 k protein‑coding genes, but not for 3.8 k RNA genes or 1.9 k pseudogenes (R = 0.43–0.54), suggesting redistribution of mRNAs upon platelet shedding from megakaryocytes. (iii) Copy numbers of 3.5 k proteins that were restricted in size by the corresponding transcript levels (iv) Near complete coverage of identifed proteins in the relevant transcriptome (log2fpkm > 0.20) except for plasma‑derived secretory proteins, pointing to adhesion and uptake of such proteins. (v) Underrepresentation in the identifed proteome of nuclear‑related, membrane and signaling proteins, as well proteins with low‑level transcripts.
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Anti-CHMP5 Antibody (ARG40721)
Product datasheet [email protected] ARG40721 Package: 50 μg anti-CHMP5 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes CHMP5 Tested Reactivity Hu Tested Application WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name CHMP5 Antigen Species Human Immunogen Recombinant protein corresponding to aa. 1-219 of Human CHMP5. Conjugation Un-conjugated Alternate Names hVps60; Charged multivesicular body protein 5; SNF7 domain-containing protein 2; HSPC177; CGI-34; Vps60; PNAS-2; Chromatin-modifying protein 5; Vacuolar protein sorting-associated protein 60; SNF7DC2; C9orf83 Application Instructions Application table Application Dilution WB 2 µg/ml Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 25 kDa Observed Size 25 kDa Properties Form Liquid Purification Caprylic acid ammonium sulfate precipitation. Buffer 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol. Preservative 0.03% Proclin 300 Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. www.arigobio.com 1/2 Bioinformation Gene Symbol CHMP5 Gene Full Name charged multivesicular body protein 5 Background CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs).
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Interactome of SARS-Cov-2 / Ncov19 Modulated Host Proteins with Computationally Predicted Ppis
Interactome of SARS-CoV-2 / nCoV19 modulated host proteins with computationally predicted PPIs Kalyani B. Karunakaran1, N. Balakrishnan1 and Madhavi K. Ganapathiraju2,3* 1Supercomputer Education and Research Centre, Indian Institute of Science, Bangalore, 560 012, India 2Department of Biomedical Informatics, School of Medicine, and 3Intelligent Systems Program, School of Computing and Information, University of Pittsburgh, Pittsburgh, USA Correspondence should be sent to [email protected] Corresponding author: Dr. Madhavi K. Ganapathiraju Department of Biomedical Informatics, University of Pittsburgh 5607 Baum Blvd, Suite 501 Pittsburgh, PA 15206, USA Email: [email protected] Phone: 412-648-9552 Fax: 412-624-5310 Running title: Computationally predicted PPIs of SARS-CoV-2 modulated host proteins Highlights • 1,941 novel interactions of proteins targeted by SARS-CoV-2 (‘host proteins’) are predicted with HiPPIP, our computational model. • The interactome is made available via a web-server Wiki-Corona (http://hagrid.dbmi.pitt.edu/corona). o It is searchable by protein IDs and annotations. o More interestingly, it is searchable with queries like “Show PPIs where one protein has to do with 'virus' and the other protein has to do with 'pulmonary'.” • The interactome is made available as downloadable network files to facilitate future systems biology studies. • Analysis of the interactome embedded with novel predicted PPIs resolved the apparent disconnect between two recent studies (transcriptional with 120 genes and proteomic with 332 genes) by showing that although they shared only two common genes, there are many direct PPIs between them and several shared common interactors. • The interactome also showed connections between host-proteins of SARS-1 and SARS-2 coronaviruses, and the proteins common to both coronaviruses were enriched for mitochondrial proteins.
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Protein 5 Human Recombinant
DATA SHEET Charged Multivesicular Body Protein 5 Human Recombinant Item Number rAP-3001 Synonyms Charged Multivesicular Body Protein 5, SNF7 Domain-Containing Protein 2, Chromosome 9 Open Reading Frame 83, Chromatin-Modifying Protein 5, Apoptosis-Related Protein PNAS-2, Vacuolar Protein Sorting- Associated Protein 60, Chromatin Modifying Protein 5, HS Description CHMP5 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-219) and having a molecular mass of 27.0 kDa.CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Uniprot Accesion Number Q9NZZ3 Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSH MNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLE- AE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS Source E.coli. Physical Appearance Sterile Filtered colorless solution. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at - and Stability 20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. Formulation and Purity The CHMP5 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 30% glycerol. Greater than 80% as determined by SDS-PAGE. Application Solubility Biological Activity Shipping Format and Condition Lyophilized powder at room temperature. Optimal dilutions should be determined by each laboratory for each application. The listed dilutions are for recommendation only and the final condi- tions should be optimized by the ender users! This product is sold for Research Use Only Angio-Proteomie | 11 Park Drive, Suite 12 Boston, MA 02215, USA | Tel: 001‐617‐549‐2665 | Fax: 001‐480‐247‐4337 | Email： [email protected] .
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Frequent Silence of Chromosome 9P, Homozygous DOCK8, DMRT1 and DMRT3 Deletion at 9P24.3 in Squamous Cell Carcinoma of the Lung
327-335.qxd 21/6/2010 10:37 Ì ™ÂÏ›‰·327 INTERNATIONAL JOURNAL OF ONCOLOGY 37: 327-335, 2010 327 Frequent silence of chromosome 9p, homozygous DOCK8, DMRT1 and DMRT3 deletion at 9p24.3 in squamous cell carcinoma of the lung JI UN KANG1, SUN HOE KOO2, KYE CHUL KWON2 and JONG WOO PARK2 1Department of Pathology, Columbia University Medical Center, New York, NY 10032, USA; 2Department of Laboratory Medicine, Chungnam National University College of Medicine, Daejeon 301-721, Republic of Korea Received February 2, 2010; Accepted March 24, 2010 DOI: 10.3892/ijo_00000681 Abstract. Chromosomal alterations are a major genomic the 2 main types of NSCLC, namely adenocarcinoma and force contributing to the development of lung cancer. We squamous cell carcinoma (SCC), account for over half of the subjected 22 cases of squamous cell carcinoma of the lung NSCLC cases. SCC of the lung is characterized by a complex (SCC) to whole-genome microarray-CGH (resolution, 1 Mb) pattern of cytogenetic and molecular genetic changes; chromo- to identify critical genetic landmarks that might be important somal aberrations are a hallmark of cancer cells and are highly mediators in the formation or progression of SCC. On a prevalent in SCC (2). Although many studies have been genome-wide profile, copy number losses (log2 ratio <-0.25) performed to evaluate the genetic events associated with the on chromosome 9p occurred in 72.7% (16/22) of the SCC development and progression of SCC (3,4), its molecular cases, and the delineated minimal common region was mechanism still remains to be understood, and identification 9p24.3-p21.1.
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Synthesis of UMI ( 8 Bases )
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Detection of H3k4me3 Identifies Neurohiv Signatures, Genomic
viruses Article Detection of H3K4me3 Identifies NeuroHIV Signatures, Genomic Effects of Methamphetamine and Addiction Pathways in Postmortem HIV+ Brain Specimens that Are Not Amenable to Transcriptome Analysis Liana Basova 1, Alexander Lindsey 1, Anne Marie McGovern 1, Ronald J. Ellis 2 and Maria Cecilia Garibaldi Marcondes 1,* 1 San Diego Biomedical Research Institute, San Diego, CA 92121, USA; [email protected] (L.B.); [email protected] (A.L.); [email protected] (A.M.M.) 2 Departments of Neurosciences and Psychiatry, University of California San Diego, San Diego, CA 92103, USA; [email protected] * Correspondence: [email protected] Abstract: Human postmortem specimens are extremely valuable resources for investigating trans- lational hypotheses. Tissue repositories collect clinically assessed specimens from people with and without HIV, including age, viral load, treatments, substance use patterns and cognitive functions. One challenge is the limited number of specimens suitable for transcriptional studies, mainly due to poor RNA quality resulting from long postmortem intervals. We hypothesized that epigenomic Citation: Basova, L.; Lindsey, A.; signatures would be more stable than RNA for assessing global changes associated with outcomes McGovern, A.M.; Ellis, R.J.; of interest. We found that H3K27Ac or RNA Polymerase (Pol) were not consistently detected by Marcondes, M.C.G. Detection of H3K4me3 Identifies NeuroHIV Chromatin Immunoprecipitation (ChIP), while the enhancer H3K4me3 histone modification was Signatures, Genomic Effects of abundant and stable up to the 72 h postmortem. We tested our ability to use H3K4me3 in human Methamphetamine and Addiction prefrontal cortex from HIV+ individuals meeting criteria for methamphetamine use disorder or not Pathways in Postmortem HIV+ Brain (Meth +/−) which exhibited poor RNA quality and were not suitable for transcriptional profiling.
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Rabbit Anti-CHMP5/FITC Conjugated Antibody-SL13914R-FITC
SunLong Biotech Co.,LTD Tel: 0086-571- 56623320 Fax:0086-571- 56623318 E-mail:[email protected] www.sunlongbiotech.com Rabbit Anti-CHMP5/FITC Conjugated antibody SL13914R-FITC Product Name: Anti-CHMP5/FITC Chinese Name: FITC标记的染色质修饰蛋白CHMP5抗体 C9orf83; CGI 34; Charged multivesicular body protein 5; CHMP 5; chmp5; CHMP5_HUMAN; Chromatin modifying protein 5; Chromatin-modifying protein 5; Alias: Chromosome 9 open reading frame 83; HGNC:26942; HSPC177; hVps60; PNAS 2; SNF7 domain containing protein 2; SNF7 domain-containing protein 2; SNF7DC2; Vacuolar protein sorting 60; Vacuolar protein sorting-associated protein 60; Vps60. Organism Species: Rabbit Clonality: Polyclonal React Species: Human,Mouse,Rat,Pig,Cow,Rabbit,Sheep, ICC=1:50-200IF=1:50-200 Applications: not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. Molecular weight: 25kDa Form: Lyophilized or Liquid Concentration: 1mg/ml immunogen: KLH conjugated synthetic peptide derived from human CHMP5 Lsotype: IgGwww.sunlongbiotech.com Purification: affinity purified by Protein A Storage Buffer: 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year Storage: when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. background: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) Product Detail: formation and sorting of endosomal cargo proteins into MVBs.
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Proteomic Analysis Uncovers Measles Virus Protein C
bioRxiv preprint doi: https://doi.org/10.1101/2020.05.08.084418; this version posted May 9, 2020. The copyright holder for this preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission. Proteomic Analysis Uncovers Measles Virus Protein C Interaction with p65/iASPP/p53 Protein Complex Alice Meignié1,2*, Chantal Combredet1*, Marc Santolini 3,4, István A. Kovács4,5,6, Thibaut Douché7, Quentin Giai Gianetto 7,8, Hyeju Eun9, Mariette Matondo7, Yves Jacob10, Regis Grailhe9, Frédéric Tangy1**, and Anastassia V. Komarova1, 10** 1 Viral Genomics and Vaccination Unit, Department of Virology, Institut Pasteur, CNRS UMR-3569, 75015 Paris, France 2 Université Paris Diderot, Sorbonne Paris Cité, Paris, France 3 Center for Research and Interdisciplinarity (CRI), Université de Paris, INSERM U1284 4 Network Science Institute and Department of Physics, Northeastern University, Boston, MA 02115, USA 5 Department of Physics and Astronomy, Northwestern University, Evanston, IL 60208-3109, USA 6 Department of Network and Data Science, Central European University, Budapest, H-1051, Hungary 7 Proteomics platform, Mass Spectrometry for Biology Unit (MSBio), Institut Pasteur, CNRS USR 2000, Paris, France. 8 Bioinformatics and Biostatistics Hub, Computational Biology Department, Institut Pasteur, CNRS USR3756, Paris, France 9 Technology Development Platform, Institut Pasteur Korea, Seongnam-si, Republic of Korea 10 Laboratory of Molecular Genetics of RNA Viruses, Institut Pasteur, CNRS UMR-3569,
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CHMP5 Is Essential for Late Endosome Function and Down-Regulation Of
Published Online: 27 March, 2006 | Supp Info: http://doi.org/10.1083/jcb.200509041 JCB: ARTICLE Downloaded from jcb.rupress.org on May 8, 2019 CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis Jae-Hyuck Shim,1,2 Changchun Xiao,1,2 Matthew S. Hayden,1,2 Ki-Young Lee,1,2 E. Sergio Trombetta,3 Marc Pypaert,3 Atsuki Nara,4 Tamotsu Yoshimori,4 Bettina Wilm,5 Hediye Erdjument-Bromage,6 Paul Tempst,6 Brigid L.M. Hogan,5 Ira Mellman,3 and Sankar Ghosh1,2 1Section of Immunobiology, 2Department of Molecular Biophysics and Biochemistry, and 3Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520 4Division of Cell Genetics, National Institute for Genetics, Mishima, 411-8540, Japan 5Department of Cell Biology, Vanderbilt University Medical Center, Nashville, TN 37232 6Memorial Sloan-Kettering Cancer Center, New York, NY 10021 harged MVB protein 5 (CHMP5) is a coiled coil characteristic of late endosomes and lysosomes. This is in protein homologous to the yeast Vps60/Mos10 contrast to ESCRT-III mutants in yeast, which are defective gene and other ESCRT-III complex members, in multivesicular body (MVB) formation. The degradative C − − although its precise function in either yeast or mammalian capacity of Chmp5 / cells was reduced, and undigested cells is unknown. We deleted the CHMP5 gene in mice, proteins from multiple pathways accumulated in enlarged resulting in a phenotype of early embryonic lethality, MVBs that failed to trafﬁ c their cargo to lysosomes. There- reﬂ ecting defective late endosome function and dysre- fore, CHMP5 regulates late endosome function down- gulation of signal transduction.
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