Protein 5 Human Recombinant

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DATA SHEET Charged Multivesicular Body Protein 5 Human Recombinant Item Number rAP-3001 Synonyms Charged Multivesicular Body Protein 5, SNF7 Domain-Containing Protein 2, Chromosome 9 Open Reading Frame 83, Chromatin-Modifying Protein 5, Apoptosis-Related Protein PNAS-2, Vacuolar Protein Sorting- Associated Protein 60, Chromatin Modifying Protein 5, HS Description CHMP5 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-219) and having a molecular mass of 27.0 kDa.CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Uniprot Accesion Number Q9NZZ3 Amino Acid Sequence MGSSHHHHHH SSGLVPRGSH MGSH MNRLFG KAKPKAPPPS LTDCIGTVDS RAESIDKKIS RLDAELVKYK DQIKKMREGP AKNMVKQKAL RVLKQKRMYE QQRDNLAQQS FNMEQANYTI QSLKDTKTTV DAMKLGVKEM KKAYKQVKID QIEDLQDQLE DMMEDANEIQ EALSRSYGTP ELDEDDLE- AE LDALGDELLA DEDSSYLDEA ASAPAIPEGV PTDTKNKDGV LVDEFGLPQI PAS Source E.coli. Physical Appearance Sterile Filtered colorless solution. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at - and Stability 20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. Formulation and Purity The CHMP5 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 30% glycerol. Greater than 80% as determined by SDS-PAGE. Application Solubility Biological Activity Shipping Format and Condition Lyophilized powder at room temperature. Optimal dilutions should be determined by each laboratory for each application. The listed dilutions are for recommendation only and the final condi- tions should be optimized by the ender users! This product is sold for Research Use Only Angio-Proteomie | 11 Park Drive, Suite 12 Boston, MA 02215, USA | Tel: 001‐617‐549‐2665 | Fax: 001‐480‐247‐4337 | Email： [email protected] .

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CHMP5 Antibody (C-Term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # Ap18536b
10320 Camino Santa Fe, Suite G San Diego, CA 92121 Tel: 858.875.1900 Fax: 858.622.0609 CHMP5 Antibody (C-term) Affinity Purified Rabbit Polyclonal Antibody (Pab) Catalog # AP18536b Specification CHMP5 Antibody (C-term) - Product Information Application WB,E Primary Accession Q9NZZ3 Other Accession Q4QQV8, Q9D7S9, NP_057494.3 Reactivity Human Predicted Mouse, Rat Host Rabbit Clonality Polyclonal Isotype Rabbit Ig Antigen Region 178-204 CHMP5 Antibody (C-term) - Additional Information CHMP5 Antibody (C-term) (Cat. #AP18536b) western blot analysis in MDA-MB231 cell line Gene ID 51510 lysates (35ug/lane).This demonstrates the Other Names CHMP5 antibody detected the CHMP5 protein Charged multivesicular body protein 5, (arrow). Chromatin-modifying protein 5, SNF7 domain-containing protein 2, Vacuolar protein sorting-associated protein 60, CHMP5 Antibody (C-term) - Background Vps60, hVps60, CHMP5, C9orf83, SNF7DC2 CHMP5 belongs to the chromatin-modifying Target/Specificity protein/charged This CHMP5 antibody is generated from multivesicular body protein (CHMP) family. rabbits immunized with a KLH conjugated These proteins are synthetic peptide between 178-204 amino components of ESCRT-III (endosomal sorting acids from the C-terminal region of human complex required for CHMP5. transport III), a complex involved in degradation of surface Dilution receptor proteins and formation of endocytic WB~~1:1000 multivesicular bodies (MVBs). Some CHMPs have both nuclear and Format cytoplasmic/vesicular Purified polyclonal antibody supplied in PBS distributions, and one such CHMP, CHMP1A with 0.09% (W/V) sodium azide. This (MIM 164010), is required antibody is purified through a protein A for both MVB formation and regulation of cell column, followed by peptide affinity cycle progression purification.
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32-3532: CHMP5 Recombinant Protein Description Product Info
9853 Pacific Heights Blvd. Suite D. San Diego, CA 92121, USA Tel: 858-263-4982 Email: [email protected] 32-3532: CHMP5 Recombinant Protein Charged Multivesicular Body Protein 5,SNF7 Domain-Containing Protein 2,Chromosome 9 Open Reading Alternative Frame 83,Chromatin-Modifying Protein 5,Apoptosis-Related Protein PNAS-2,Vacuolar Protein Sorting- Name : Associated Protein 60,Chromatin Modifying Protein Description Source : E.coli. CHMP5 Human Recombinant produced in E. coli is a single polypeptide chain containing 243 amino acids (1-219) and having a molecular mass of 27.0 kDa.CHMP5 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Charged Multivesicular Body Protein 5 (CHMP5) is a member of the chromatin- modifying protein/charged multivesicular body protein (CHMP) family. These proteins comprise the ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and development of endocytic multivesicular bodies (MVBs). Certain CHMPs have both nuclear and cytoplasmic/vesicular distributions, and one such CHMP the CHMP1A, is essential for both MVB formation and regulation of cell cycle progression. Product Info Amount : 20 µg Purification : Greater than 80% as determined by SDS-PAGE. The CHMP5 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 30% Content : glycerol. Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of Storage condition : time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
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Downloaded Per Proteome Cohort Via the Web- Site Links of Table 1, Also Providing Information on the Deposited Spectral Datasets
www.nature.com/scientificreports OPEN Assessment of a complete and classifed platelet proteome from genome‑wide transcripts of human platelets and megakaryocytes covering platelet functions Jingnan Huang1,2*, Frauke Swieringa1,2,9, Fiorella A. Solari2,9, Isabella Provenzale1, Luigi Grassi3, Ilaria De Simone1, Constance C. F. M. J. Baaten1,4, Rachel Cavill5, Albert Sickmann2,6,7,9, Mattia Frontini3,8,9 & Johan W. M. Heemskerk1,9* Novel platelet and megakaryocyte transcriptome analysis allows prediction of the full or theoretical proteome of a representative human platelet. Here, we integrated the established platelet proteomes from six cohorts of healthy subjects, encompassing 5.2 k proteins, with two novel genome‑wide transcriptomes (57.8 k mRNAs). For 14.8 k protein‑coding transcripts, we assigned the proteins to 21 UniProt‑based classes, based on their preferential intracellular localization and presumed function. This classifed transcriptome‑proteome profle of platelets revealed: (i) Absence of 37.2 k genome‑ wide transcripts. (ii) High quantitative similarity of platelet and megakaryocyte transcriptomes (R = 0.75) for 14.8 k protein‑coding genes, but not for 3.8 k RNA genes or 1.9 k pseudogenes (R = 0.43–0.54), suggesting redistribution of mRNAs upon platelet shedding from megakaryocytes. (iii) Copy numbers of 3.5 k proteins that were restricted in size by the corresponding transcript levels (iv) Near complete coverage of identifed proteins in the relevant transcriptome (log2fpkm > 0.20) except for plasma‑derived secretory proteins, pointing to adhesion and uptake of such proteins. (v) Underrepresentation in the identifed proteome of nuclear‑related, membrane and signaling proteins, as well proteins with low‑level transcripts.
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Anti-CHMP5 Antibody (ARG40721)
Product datasheet [email protected] ARG40721 Package: 50 μg anti-CHMP5 antibody Store at: -20°C Summary Product Description Rabbit Polyclonal antibody recognizes CHMP5 Tested Reactivity Hu Tested Application WB Host Rabbit Clonality Polyclonal Isotype IgG Target Name CHMP5 Antigen Species Human Immunogen Recombinant protein corresponding to aa. 1-219 of Human CHMP5. Conjugation Un-conjugated Alternate Names hVps60; Charged multivesicular body protein 5; SNF7 domain-containing protein 2; HSPC177; CGI-34; Vps60; PNAS-2; Chromatin-modifying protein 5; Vacuolar protein sorting-associated protein 60; SNF7DC2; C9orf83 Application Instructions Application table Application Dilution WB 2 µg/ml Application Note * The dilutions indicate recommended starting dilutions and the optimal dilutions or concentrations should be determined by the scientist. Calculated Mw 25 kDa Observed Size 25 kDa Properties Form Liquid Purification Caprylic acid ammonium sulfate precipitation. Buffer 0.01M PBS (pH 7.4), 0.03% Proclin 300 and 50% Glycerol. Preservative 0.03% Proclin 300 Stabilizer 50% Glycerol Storage instruction For continuous use, store undiluted antibody at 2-8°C for up to a week. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. Suggest spin the vial prior to opening. The antibody solution should be gently mixed before use. Note For laboratory research only, not for drug, diagnostic or other use. www.arigobio.com 1/2 Bioinformation Gene Symbol CHMP5 Gene Full Name charged multivesicular body protein 5 Background CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family. These proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies (MVBs).
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Synthesis of UMI ( 8 Bases )
TAN TANTA CU O USLA 20180030515A1DA MATA MATA MALTA MARTINI ( 19) United States (12 ) Patent Application Publication (10 ) Pub. No. : US 2018/ 0030515 A1 Regev et al. (43 ) Pub . Date : Feb . 1 . 2018 ( 54 ) DROPLET -BASED METHOD AND Related U . S . Application Data APPARATUS FOR COMPOSITE (63 ) Continuation - in -part of application No . PCT/ SINGLE - CELL NUCLEIC ACID ANALYSIS US2015 / 049178 , filed on Sep . 9 , 2015 . ( 71) Applicants : The Broad Institute Inc ., Cambridge, (60 ) Provisional application No . 62 /048 ,227 , filed on Sep . MA (US ) ; Massachusetts Institute of 9 , 2014 , provisional application No. 62 / 146 ,642 , filed Technology , Cambridge , MA (US ) ; on Apr. 13 , 2015 . President and Fellows of Harvard College, Cambridge, MA (US ) Publication Classification (51 ) Int . CI. (72 ) Inventors : Aviv Regev , Cambridge, MA (US ) ; C120 1 /68 (2006 .01 ) Evan Zane MACOSKO , Cambridge , GO6K 19 / 06 ( 2006 . 01 ) MA (US ) ; Steven Andrew ( 2006 .01 ) MCCARROLL , Cambridge , MA (US ) ; C12N 15 / 10 Alexander K . SHALEK , Cambridge, (52 ) U . S . CI. MA (US ) ; Anindita BASU , Cambridge , CPC .. .. C12Q 1/ 6809 ( 2013. 01 ) ; C12N 15 / 1096 MA (US ) ; Christopher B . FORD , (2013 . 01 ) ; C12Q 1 /6869 ( 2013 .01 ) ; C12Q Cambridge, MA (US ) ; Hongkun 1 /6834 ( 2013. 01 ) ; G06K 19 / 06 ( 2013. 01 ) PARK , Lexington , MA (US ) ; David A . (57 ) ABSTRACT WEITZ , Bolton , MA (US ) The present invention generally relates to a combination of molecular barcoding and emulsion - based microfluidics to (21 ) Appl. No. : 15 / 453 ,405 isolate , lyse , barcode , and prepare nucleic acids from indi (22 ) Filed : Mar. 8 , 2017 vidual cells in a high - throughput manner . Synthesis of UMI ( 8 bases) 8 rounds of synthesis • Millions of the same cell barcode per bead • 48 differentmolecular barcodes (UMIS ) per bead Patent Application Publication Feb .
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Detection of H3k4me3 Identifies Neurohiv Signatures, Genomic
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Rabbit Anti-CHMP5/FITC Conjugated Antibody-SL13914R-FITC
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CHMP5 Polyclonal Antibody
CHMP5 polyclonal antibody Catalog # : PAB6771 規格 : [ 100 ug ] List All Specification Application Image Product Goat polyclonal antibody raised against synthetic peptide of CHMP5. Western Blot (Cell lysate) Description: Immunogen: A synthetic peptide corresponding to human CHMP5. Sequence: C-NKDGVLVDEFGLPQ enlarge Host: Goat ELISA Theoretical MW 24.5 (kDa): Reactivity: Human Form: Liquid Purification: Antigen affinity purification Concentration: 0.5 mg/mL Quality Control Antibody Reactive Against Synthetic Peptide. Testing: Recommend ELISA (1:8000) Usage: Western Blot (1-3 ug/mL) The optimal working dilution should be determined by the end user. Storage Buffer: In Tris saline, pH 7.3 (0.5% BSA, 0.02% sodium azide) Storage Store at -20°C. Instruction: Aliquot to avoid repeated freezing and thawing. Note: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only. Datasheet: Download Publication Reference 1. The role of LIP5 and CHMP5 in multivesicular body formation and HIV-1 budding in mammalian cells. Ward DM, Vaughn MB, Shiflett SL, White PL, Pollock AL, Hill J, Schnegelberger R, Sundquist WI, Kaplan J.J Biol Chem. 2005 Mar 18;280(11):10548-55. Epub 2005 Jan 11. Applications Western Blot (Cell lysate) Page 1 of 2 2017/2/16 CHMP5 polyclonal antibody (Cat # PAB6771) (0.5 ug/mL) staining of K-562 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. ELISA Gene Information Entrez GeneID: 51510 Protein NP_057494.2 Accession#: Gene Name: CHMP5 Gene Alias: C9orf83,CGI-34,HSPC177,PNAS-2,SNF7DC2 Gene chromatin modifying protein 5 Description: Omim ID: 610900 Gene Ontology: Hyperlink Gene Summary: CHMP5 belongs to the chromatin-modifying protein/charged multivesicular body protein (CHMP) family.
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Proteomic Analysis Uncovers Measles Virus Protein C
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CHMP5 Is Essential for Late Endosome Function and Down-Regulation Of
Published Online: 27 March, 2006 | Supp Info: http://doi.org/10.1083/jcb.200509041 JCB: ARTICLE Downloaded from jcb.rupress.org on May 8, 2019 CHMP5 is essential for late endosome function and down-regulation of receptor signaling during mouse embryogenesis Jae-Hyuck Shim,1,2 Changchun Xiao,1,2 Matthew S. Hayden,1,2 Ki-Young Lee,1,2 E. Sergio Trombetta,3 Marc Pypaert,3 Atsuki Nara,4 Tamotsu Yoshimori,4 Bettina Wilm,5 Hediye Erdjument-Bromage,6 Paul Tempst,6 Brigid L.M. Hogan,5 Ira Mellman,3 and Sankar Ghosh1,2 1Section of Immunobiology, 2Department of Molecular Biophysics and Biochemistry, and 3Department of Cell Biology, Ludwig Institute for Cancer Research, Yale University School of Medicine, New Haven, CT 06520 4Division of Cell Genetics, National Institute for Genetics, Mishima, 411-8540, Japan 5Department of Cell Biology, Vanderbilt University Medical Center, Nashville, TN 37232 6Memorial Sloan-Kettering Cancer Center, New York, NY 10021 harged MVB protein 5 (CHMP5) is a coiled coil characteristic of late endosomes and lysosomes. This is in protein homologous to the yeast Vps60/Mos10 contrast to ESCRT-III mutants in yeast, which are defective gene and other ESCRT-III complex members, in multivesicular body (MVB) formation. The degradative C − − although its precise function in either yeast or mammalian capacity of Chmp5 / cells was reduced, and undigested cells is unknown. We deleted the CHMP5 gene in mice, proteins from multiple pathways accumulated in enlarged resulting in a phenotype of early embryonic lethality, MVBs that failed to trafﬁ c their cargo to lysosomes. There- reﬂ ecting defective late endosome function and dysre- fore, CHMP5 regulates late endosome function down- gulation of signal transduction.
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