SEWPROF Roundtable Spain

John Ramsey TICTAC Communications Ltd St. George’s, University of London London SW17 0RE New Psychoactive Substances in Wastewater

TICTAC’s contribution

• ER – Dr. Bram Miserez

• Discovery and characterisation of NPS • Test purchases • Amnesty bins • Police seizures • Prisons

• Pooled urine collection • Dissemination of samples • Analysis of results New Psychoactive Substances in Wastewater

 TICTAC Communications Ltd. Typical UK “head shop” offering April 2013

Rock Stars Tablet 5-MeO-DALT Rockets Capsule AMT Tablet AMT Dutchy Herb 5F-AKB48, STS-135, MAM-2201 E-Scape Tablet 5-MAPB, 5/6-APB Puff Adder Herb 5F-AKB48, AKB48 Chillem Blue Herb 5F-AKB48, AKB48 Poke Powder , Methiopropamine Dust Till Dawn Powder Benzocaine, Methiopropamine Charly Sheen Powder Lidocaine, Methiopropamine, MDAI Clockwork Orange Herb 5F-AKB48 Gogaine Powder Lidocaine, Methiopropamine, Ethylphenidate Bomb Powder Methiopropamine, Benzocaine Magic Crystals Powder Benzocaine, , Ethylphenidate China White Powder Methiopropamine, Benzocaine Barry White Powder Methiopropamine, MDPV, Lidocaine, 2-Aminoindane Methylone Blue Genie Tablet Methiopropamine, 5/6-APB Jack and Jills Tablet Ethylphenidate Disco Biscuts Tablet AMT Green Beans Capsule Methiopropamine, 5/6-APB 6APB Capsule Blast Tablet 5-MeO-DALT Fury Xtreme Capsule 5/6-APB, Methiopropamine Pink Panters Capsule Methiopropamine Purple Bomb Tablet Ethylphenidate, 5-Meo-DALT, Methiopropamine Jumping Beans Tablet Methiopropamine AMT Matrix Fury Tablet Methoxetamine

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater derivatives

 Methylphenidate (Ritalin ®)  Ethylphenidate  3,4-CTMP Methylphenidate   HDMP-28 (methylnaphthidate)

IsoPropylphenidate

3,4-CTMP Methylnaphthidate Ethylphenidate

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater

This means that it will be unlawful to supply, possess with intent to supply, produce, and import or export ethylphenidate, 3,4-dichloromethylphenidate, methylnaphthidate (HDMP-28), isopropylphenidate and , and their simple derivatives, except under licence issued by the Home Office.

 TICTAC Communications Ltd. The driver for change new compounds are used as drugs because they fall outside legislation

the law catches up

The consequence is that vulnerable young people are exposed to a succession of compounds that have never been tested for safety

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater The response ?

B2 / M1M

analogue (3,4-methdioxy variant)  1-(hexahydrobenzo[d][1,3]dioxol-5-yl)-N-methylpropan-2-amine  500g B2 - £1500 or 1kg B2 - £2500

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater

Synthetic cannabinoids detected by TICTAC March 2015

 5-fluoro NPB-22  URB597  5-fluoro AB-PINACA  AM2201 benzimidazole analog  THJ 018  AB-PINACA  JWH-018 indazole analog  ADB-FUBINACA  SDB-006  URB754  5-fluoro MN-18  THJ2201  FUB-PB-22  A-836339  EG 018  MDMB-CHMICA  5-fluoro AMB  MDMB-CHMINACA

 TICTAC Communications Ltd. MDMB-CHMINACA

URB754

endogenous agonist of the central cannabinoid (CB1) receptor

A-836339 MDMB-CHMICA 5F-AB-PINACA

higher affinity for the peripheral CB2 receptor analgesic, anti-inflammatory, and anti-hyperalgesic effects in mice

 TICTAC Communications Ltd. Data from:- Dr. María Ibáñez Martínez

O 5F-AB-PINA

O NH2 NH

N [M+H]+ N [M+Na]+

[M+Na]+ [M+H]+

C18H25FN4O2

F

31424 14 5F-AB-PINACA  single peak by GCMS

Internal standards

 TICTAC Communications Ltd. New Psychoactive Substances in Wastewater

The challenge to SEWPROF is to move faster

M  Monitor market half life of new compounds TN 1/2 UK  Time from first detection to disappearance from the local market  Characterise new compound  Minimum data set * Pharmacology / Toxicology * Dose * Duration of action  Distribute standards  Access or guess human/bacterial metabolism  Access stability  Develop and validate analytical method  Implement

 TICTAC Communications Ltd.