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2011 ANNUAL REPORT Untitled-1 1 7/17/12 10:58 AM ANNUAL REPORT 2011 © 2012 by Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory One Bungtown Road Cold Spring Harbor, New York 11724 www.cshl.edu Managing Editors Phil Renna, Dagnia Zeidlickis Production Editor Rena Steuer Science Writing Peter Tarr Copy Editors Dorothy Brown, Rena Steuer Production Manager Denise Weiss Desktop Editor Susan Schaefer Nonscientific Photography Constance Brukin, Gina Motisi Cover Designers Margot Bennett, Denise Weiss Book Designer Emily Harste Front cover: Bronze Helix, 1993. Artist, Charles J. Reina; photography, Gina Motisi Contents Officers of the Corporation and Board of Trustees iv–v Governance vi Committees of the Board vii Evelyn H. Lauder (1936–2011) viii PRESIDENT’S REPORT 1 Highlights of the Year 7 CHIEF OPERATING OFFICER’S REPORT 25 Long-term Service 27 RESEARCH 29 Cancer: Gene Regulation and Cell Proliferation 31 Cancer: Genetics 53 Cancer: Signal Transduction 82 Neuroscience 111 Plant Genetics 166 Genomics 193 Quantitative Biology 219 Quantitative Biology Fellows 228 CSHL Fellows 232 Author Index 235 WATSON SCHOOL OF BIOLOGICAL SCIENCES 239 Dean’s Report 241 Spring Curriculum 257 Fall Curriculum 260 Postdoctoral Program 265 Undergraduate Research Program 270 Partners for the Future 273 COLD SPRING HARBOR LABORATORY MEETINGS AND COURSES 275 Academic Affairs 276 Symposium on Quantitative Biology 278 Meetings 281 Postgraduate Courses 334 Seminars 395 BANBURY CENTER 397 Executive Director’s Report 399 Meetings 402 DNA LEARNING CENTER 445 Executive Director’s Report 447 2011 Workshops, Meetings, and Collaborations 461 Sites of Major Faculty Workshops 1985–2011 465 COLD SPRING HARBOR LABORATORY PRESS 469 2011 Press Publications 470 Executive Director’s Report 471 FINANCE 473 Financial Statements 474 Financial Support of the Laboratory 477 Corporate Sponsor Program for Meetings Support 487 Development 488 LABORATORY MANAGEMENT 497 iii Officers of the Corporation Jamie C. Nicholls, Chairman Edward Travaglianti, Secretary Robert D. Lindsay, Vice Chairman Bruce Stillman, Ph.D., President Marilyn H. Simons, Ph.D., Vice Chairman W. Dillaway Ayres, Jr., Chief Operating Officer Leo A. Guthart, Treasurer Board of Trustees Individual Trustees David Boies Jacob Goldfield Leo A. Guthart Laurie J. Landeau, V.M.D. Thomas D. Lehrman Chairman, Boies, Schiller & New York, New York Founder and CEO, Topspin General Manager, Listowel, Inc. CEO, Alta Investors Flexner LLP Partners, Treasurer of the Board Stephen M. Lessing Robert D. Lindsay Howard L. Morgan Jamie C. Nicholls Louise M. Parent Managing Director, Co-Managing Partner, Partner, First Round Capital, New York, New York Executive Vice President and Barclay’s Capital Lindsay Goldberg Director, Idealab Chairman of the Board General Counsel, American Express Company John C. Phelan Thomas C. Quick William S. Robertson David M. Rubenstein George Sard Managing Partner, Palm Beach, Florida Chairman, Robertson Foun- Cofounder and Managing Chairman and CEO, MSD Capital, L.P. dation for Government Director, The Carlyle Group Sard Verbinnen & Co. Alan Seligson Marilyn H. Simons, Ph.D. Dinakar Singh Andrew Solomon Alan C. Stephenson NAK International President, The Simons Foundation, Founding Partner, Writer, Lecturer in Psychiatry, Chapel Hill, North Carolina Vice Chairman of the Board TPG-Axon Capital Weill-Cornell Medical College iv Individual Trustees (continued) James M. Stone, Ph.D. Jerome Swartz, Ph.D. Paul J. Taubman Edward Travaglianti Sandra Tytel Roy J. Zuckerberg Chairman, The Plymouth Chairman, The Swartz Co-President of Institutional President, TD Bank Mill Neck, New York Senior Director, Rock Company Foundation Securities, Morgan Stanley Long Island Goldman, Sachs & Co. Scientific Trustees Tania A. Baker, Ph.D. Michael R. Botchan, Ph.D. David Botstein, Ph.D. Charles L. Sawyers, M.D. Samuel L. Stanley, Jr., M.D. Bruce Stillman, Ph.D. E.C. Whitehead Professor of Richard and Rhoda Goldman Dis- Anthony B. Evnin Professor of Investigator, Howard Hughes President, President, Biology, Massachusetts tinguished Professor of Biochem- Genomics; Director, Lewis- Medical Institute and Chairman, Stony Brook University Cold Spring Harbor Institute of Technology, Investi- istry and Dept. Chair Molecular Sigler Institute, Princeton Human Oncology and Patho- Laboratory gator, HHMI and Cell Biology, University of University genesis Program, Memorial California, Berkeley Sloan-Kettering Cancer Center Honorary Trustees Bayard Clarkson, M.D. Norris W. Darrell Charles F. Dolan Helen Ann Dolan Lola N. Grace Memorial Sloan-Kettering Sullivan & Cromwell Cablevision Systems Corp. c/o Cablevision Systems Middle East Children’s Cancer Center, New York Corp. Institute Townsend J. Knight, Esq.* David H. Koch Evelyn H. Lauder** Mary D. Lindsay David L. Luke III Curtis, Mallet-Prevost, Koch Industries, Inc. Estee Lauder Companies, Inc. Laurel Hollow, New York New York, New York Colt & Mosle Nancy Abeles Marks Eduardo G. Mestre William R. Miller James D. Watson, Ph.D. Henry Wendt III Nancy Marks Interiors and Evercore Partners New York, New York Chancellor Emeritus, Cold Friday Harbor, Washington Carl Marks & Co., Inc. Spring Harbor Laboratory *Deceased 2012. ** Deceased 2011. Governance The Laboratory is governed by a Board of Trustees of up to 35 members that meets three or four times a year. Authority to act for the Board of Trustees between meetings is vested in the Executive Committee of the Board of Trustees. The Executive Committee is composed of the Officers of the Board and any other members who may be elected to the Executive Committee by the Board of Trustees. Additional standing and ad hoc committees are appointed by the Board of Trustees to pro- vide guidance and advice in specific areas of the Laboratory's operations. Representation on the Board of Trustees itself is divided between business and community lead- ers and scientists from major educational and research institutions. The Laboratory is chartered as an educational and research institution by the Board of Regents of the Education Department of the State of New York. It is authorized to operate a graduate pro- gram under the name “Cold Spring Harbor Laboratory, Watson School of Biological Sciences” and thereat to confer the degrees of Doctor of Philosophy (Ph.D.), Master of Science (M.S.), and Doctor of Science (Sc.D.), Honorary. It is designated as a “public charity” under Section 501(c)(3) of the Internal Revenue Code. vi Committees of the Board Academic Affairs Development Lari C. Russo Marilyn H. Simons, Ph.D. James M. Stone, Ph.D., Chair Marilyn H. Simons, Ph.D., Edward Travaglianti W. Dillaway Ayres, Jr. Chair Tania A. Baker, Ph.D. W. Dillaway Ayres, Jr. Investment Michael R. Botchan, Ph.D. David Boies Laurie J. Landeau, V.M.D. Kristina Perkin Davison John C. Phelan, Chair Thomas D. Lehrman Howard Lee Morgan, Ph.D. W. Dillaway Ayres, Jr. Bruce Stillman, Ph.D. Jamie C. Nicholls Thomas D. Lehrman Louise M. Parent Stephen M. Lessing George Sard Jamie C. Nicholls Ad Hoc DNA Learning Center Alan Seligson William S. Robertson Dinakar Singh Dinakar Singh Laurie J. Landeau, V.M.D., Chair Bruce Stillman, Ph.D. Edward Travaglianti W. Dillaway Ayres, Jr. Paul J. Taubman Paul J. Taubman Edward A. Chernoff Roy J. Zuckerberg Maria de Lesseps Suzanne Leeds Nominating David A. Micklos Executive Robert D. Lindsay, Chair Bruce Stillman, Ph.D. W. Dillaway Ayres, Jr. Jamie C. Nicholls, Chair Leo A. Guthart W. Dillaway Ayres, Jr. Jamie C. Nicholls Leo A. Guthart Audit Louise M. Parent Marilyn H. Simons, Ph.D. David Rubenstein Leo A. Guthart, Chair Bruce Stillman, Ph.D. Bruce Stillman, Ph.D. Louise M. Parent James M. Stone, Ph.D. Paul J. Taubman Alan C. Stephenson, Esq. Paul J. Taubman Roy J. Zuckerberg Edward Travaglianti Edward Travaglianti Sandra Tytel Roy J. Zuckerberg Robertson Research Fund Commercial Relations W. Dillaway Ayres, Jr. James M. Stone, Ph.D., Chair Katherine Ernst Finance W. Dillaway Ayres, Jr. Walter Goldschmidts David Botstein, Ph.D. Leo A. Guthart, Chair William Gridley John P. Maroney, Esq. W. Dillaway Ayres, Jr. Leo A. Guthart Robert Martienssen, Ph.D. Robert D. Lindsay John H. Linnartz Alan C. Stephenson, Esq. Jamie C. Nicholls Jamie C. Nicholls Bruce Stillman, Ph.D. John C. Phelan William S. Robertson Jerome Swartz, Ph.D. William S. Robertson Bruce Stillman, Ph.D. vii Evelyn H. Lauder (1936–2011) On hearing of her passing on November 12, 2011, Mike Wigler described her so compellingly: “She exemplified the best in human nature—grace and passion, intelligence and commitment, com- passion and effectiveness, humor and seriousness of purpose.” Evelyn H. Lauder was a remarkable woman. At the Laboratory, she made her mark funding Mike’s groundbreaking breast cancer research. Her relationship with him began in 1998, when he received his first grant from the Breast Cancer Research Foundation (BCRF). It was thanks to Larry Norton, who has directed the BCRF’s sci- entific activities from the start, that Evelyn’s organization was introduced to Mike and Cold Spring Harbor Laboratory. Our first BCRF grant was in 1998, including funds toward the purchase of mi- croarray equipment to support collaboration between CSHL and Memorial Sloan-Kettering Can- cer Center. The evening before BCRF’s fall 1998 research symposium, Liz and I were invited along with Mike and Deedee for dinner at Evelyn and Leonard’s spacious Fifth Avenue apartment, whose more public walls were dominated by wonderful cubist-period paintings by Pablo Picasso. The next spring, in May, Evelyn, along with BCRF donor Lillian Goldman, Larry Norton, and BCRF Executive Director Susan Hirschorn, visited CSHL to view the equipment. A plaque naming the Lillian Goldman DNA Microarray Facility was presented. During the last 14 years, CSHL has ben- efited from nearly $4 million in funding from the BCRF. At a late November 2002 fundraising evening, Liz and I, charmed by Evelyn’s aerial photos of green Caribbean hills, quickly chose Flying to Sasua to hang in our recently purchased New York City apartment. Soon after, Evelyn graciously let us elect her as one of our Honorary Trustees.
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  • Antimicrobial and Cytotoxic Properties of the Ascidians Lissoclinum

    Antimicrobial and Cytotoxic Properties of the Ascidians Lissoclinum

    Original Article 65 Pharmaceutical Sciences and Research (PSR), 5(2), 2018, 65 - 71 Antimicrobial and Cytotoxic Properties of the Ascidians Lissoclinum patella, Oxycoryna fascicularis, Didemnum molle and Botryllus schlosseri Firmansyah Karim1, Masteria Yunovilsa Putra1, Tri Aryono Hadi1, Muhammad Abrar1 1Research Center for Oceanography, Indonesian Institute of Science ABSTRACT The aim of this research was to investigate antimicrobial and cytotoxic activity of Indonesian ascidians. Extracts prepared from the Indonesian ascidians namely Lissoclinum patella, Oxycoryna fascicularis, Didemnum molle and Botryllus schlosseri were assessed for anti- microbial and cytotoxic properties. Antibacterial activity of the extracts was tested against two Gram-positive bacteria, viz. Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 25923, and three Gram-negative bacteria, viz. Escherichia coli ATCC 25922, Vibrio cholerae ATCC 14035 and Pseudomonas aeruginosa ATCC 101454 using the disk ARTICLE HISTORY diffusion test. Antifungal activity was also tested againstCandida albicans ATCC 10231 and Received: October 2017 Aspergillus niger ATCC 16404. The minimum inhibitory concentrations (MICs) of potential Revised: November 2017 ascidian extracts were determined by the microdilution technique. Cytotoxicity of the extracts Accepted: June 2018 was assessed using the brine shrimp lethality bioassay. By comparing the inhibition zones in the disk diffusion test, the most active anti-bacterial activity against Gram-positive bacteria (S. aureus and B. subtilis) was found in the crude extracts of Oxycoryna fascicularis and Didemnum molle. Lissoclinum patella extract showed the highest activity against the Gram- negative bacteria E. coli and V. cholerae. The LC50 values of the crude extracts of Lissoclinum patella, Didemnum molle, Botryllus schlosseri, and Oxycoryna fascicularis were 74.3, 97.2, 114.7 and 132.9 μg/ml, respectively.