Assessment of Insecticidal Activity of Benzylisoquinoline Alkaloids From
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Is the Mega-Diverse Genus Ocyptamus (Diptera, Syrphidae) Monophyletic
Molecular Phylogenetics and Evolution 62 (2012) 191–205 Contents lists available at SciVerse ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Is the mega-diverse genus Ocyptamus (Diptera, Syrphidae) monophyletic? Evidence from molecular characters including the secondary structure of 28S rRNA ⇑ Ximo Mengual a,c, , Gunilla Ståhls b, Santos Rojo c a Dept. of Entomology, National Museum of Natural History, Smithsonian Institution, PO Box 37012, MRC-0169, Washington, DC 20013-7012, USA b Zoological Unit, Finnish Museum of Natural History, PO Box 17, FIN-00014 University of Helsinki, Finland c Instituto Universitario CIBIO – Dpto. de Ciencias Ambientales y Recursos Naturales, Universidad de Alicante, Apdo. 99, E-03080 Alicante, Spain article info abstract Article history: Phylogenetic relationships between two New World Syrphinae taxa (Diptera, Syrphidae), i.e. the highly Received 17 March 2011 diverse genus Ocyptamus and the large genus Toxomerus, were analysed based on molecular characters. Revised 17 August 2011 The monophyly of both taxa was tested and the taxonomic status of included subgenera and species Accepted 23 September 2011 groups was examined. Toxomerus constitutes the monogeneric tribe Toxomerini with more than 140 Available online 29 September 2011 described species, while Ocyptamus (tribe Syrphini) is a very diverse genus (over 300 spp.) with multiple recognised subgenera and species groups. Sequence data from three gene regions were used: the mito- Keywords: chondrial protein-coding gene cytochrome c oxidase subunit I (COI) and the nuclear 28S and 18S ribo- Toxomerus somal RNA genes. The secondary structure of two expansion segments (D2, D3) of the ribosomal 28S Ocyptamus Monophyly RNA gene is presented for the family Syrphidae and used for the first time in a multiple sequence align- Syrphidae ment. -
The Effects of Marking Methodology on Mate Choice in Drosophila Melanogaster
ABC 2020, 7(4):492-504 Animal Behavior and Cognition DOI: https://doi.org/10.26451/abc.07.04.02.2020 ©Attribution 3.0 Unported (CC BY 3.0) PREREGISTERED REPORT The Effects of Marking Methodology on Mate Choice in Drosophila melanogaster Eirlys E. Tysall1, Matilda Q. R. Pembury Smith1*, and R. Tucker Gilman2 1Faculty of Biology, Medicine, and Health, University of Manchester 2Faculty of Science and Engineering; University of Manchester *Corresponding author (Email: [email protected]) Citation – Tysall, E. E., Pembury Smith, M. Q. R., & Gilman, R. T. (2020). The effects of marking methodology on mate choice in Drosophila melanogaster. Animal Behavior and Cognition, 7(4), 492-504. doi: https://doi.org/10.26451/abc.07.04.02.2020 Note: This paper is the final report based on an accepted peer-reviewed preregistered submission that can be found here. Abstract – Mate choice is an important source of sexual selection and a key driver of evolutionary processes including adaptation and speciation. Drosophila species have become an important model system for studying mate choice in the lab. Mate choice experiments often require the marking of individual flies to make those flies identifiable to experimenters, and several marking methods have been developed. All of these methods have the potential to affect mating behavior, but the effects of different marking methods have not been systematically quantified and compared. In this experiment, we quantified and compared the effects of five marking methods commonly used for Drosophila melanogaster: wing clipping, applying paint to the thorax, applying marker pen to the wing, dusting flies with fluorescent powder, and dyeing flies by allowing them to ingest food coloring. -
Endogenous Metabolites in Drug Discovery: from Plants to Humans
Endogenous Metabolites in Drug Discovery: from Plants to Humans Joaquim Olivés Farrés TESI DOCTORAL UPF / ANY 201 6 DIRECTOR DE LA TESI: Dr. Jordi Mestres CEXS Department The research in this T hesis has been carried out at the Systems Pharmacolo gy Group , within the Research Programme on Biomedical Informatics (GRIB) at the Parc de Recerca Biomèdica de Barcelona (PRBB). The research presented in this T hesis has been supported by Ministerio de Ciencia e Innovación project BIO2014 - 54404 - R and BIO2011 - 26669 . Printing funded by the Fundació IMIM’s program “Convocatòria d'ajuts 2016 per a la finalització de tesis doctorals de la Fundació IMIM.” Agraïments Voldria donar les gràcies a tanta gent que em fa por deixar - me ningú. Però per c omençar haig agrair en especial al meu director la tesi, Jordi Mestres, per donar - me la oportunitat de formar part del seu laboratori i poder desenvolupar aquí el treball que aquí es presenta. A més d’oferir l’ajuda necessària sempre que ha calgut. També haig de donar les gràcies a tots els companys del grup de Farmacologia de Sistemes que he anat coneguent durants tots aquests anys en què he estat aquí, en especial en Xavi, a qui li he preguntat mil coses, en Nikita, pels sdfs que m’ha anat llençant a CTL ink, i la Irene i la Cristina, que els seus treballs també m’ajuden a completar la tesis. I cal agrair també a la resta de companys del laboratori, l’Albert, la Viktoria, la Mari Carmen, l’Andreas, en George, l’Eric i l’Andreu; de Chemotargets, en Ricard i en David; i altres membres del GRIB, com són l’Alfons, en Miguel, en Pau, l’Oriol i la Carina. -
DNA Affects Ligand Binding of the Ecdysone Receptor of Anca Azoitei, Margarethe Spindler-Barth
DNA Affects Ligand Binding of the Ecdysone Receptor of Anca Azoitei, Margarethe Spindler-Barth To cite this version: Anca Azoitei, Margarethe Spindler-Barth. DNA Affects Ligand Binding of the Ecdysone Receptor of. Molecular and Cellular Endocrinology, Elsevier, 2009, 303 (1-2), pp.91. 10.1016/j.mce.2009.01.022. hal-00499113 HAL Id: hal-00499113 https://hal.archives-ouvertes.fr/hal-00499113 Submitted on 9 Jul 2010 HAL is a multi-disciplinary open access L’archive ouverte pluridisciplinaire HAL, est archive for the deposit and dissemination of sci- destinée au dépôt et à la diffusion de documents entific research documents, whether they are pub- scientifiques de niveau recherche, publiés ou non, lished or not. The documents may come from émanant des établissements d’enseignement et de teaching and research institutions in France or recherche français ou étrangers, des laboratoires abroad, or from public or private research centers. publics ou privés. Accepted Manuscript Title: DNA Affects Ligand Binding of the Ecdysone Receptor of Drosophila melanogaster Authors: Anca Azoitei, Margarethe Spindler-Barth PII: S0303-7207(09)00094-X DOI: doi:10.1016/j.mce.2009.01.022 Reference: MCE 7136 To appear in: Molecular and Cellular Endocrinology Received date: 10-11-2008 Revised date: 26-12-2008 Accepted date: 23-1-2009 Please cite this article as: Azoitei, A., Spindler-Barth, M., DNA Affects Ligand Binding of the Ecdysone Receptor of Drosophila melanogaster, Molecular and Cellular Endocrinology (2008), doi:10.1016/j.mce.2009.01.022 This is a PDF file of an unedited manuscript that has been accepted for publication. -
Direct and Widespread Role for the Nuclear Receptor Ecr in Mediating the Response to Ecdysone in Drosophila
Direct and widespread role for the nuclear receptor EcR in mediating the response to ecdysone in Drosophila Christopher M. Uyeharaa,b,c,d and Daniel J. McKaya,b,d,1 aDepartment of Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; bDepartment of Genetics, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; cCurriculum in Genetics and Molecular Biology, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599; and dIntegrative Program for Biological and Genome Sciences, The University of North Carolina at Chapel Hill, Chapel Hill, NC 27599 Edited by Lynn M. Riddiford, University of Washington, Friday Harbor, WA, and approved April 5, 2019 (received for review January 10, 2019) The ecdysone pathway was among the first experimental systems receptor) and ultraspiracle (Usp) (homolog of mammalian RXR) employed to study the impact of steroid hormones on the genome. (3). In the absence of ecdysone, EcR/Usp is nuclear localized and In Drosophila and other insects, ecdysone coordinates developmen- bound to DNA where it is thought to act as a transcriptional re- tal transitions, including wholesale transformation of the larva into pressor (4, 5). Upon ecdysone binding, EcR/Usp switches to a the adult during metamorphosis. Like other hormones, ecdysone transcriptional activator (4). Consistent with the dual regulatory controls gene expression through a nuclear receptor, which func- capacity of EcR/Usp, a variety of coactivator and corepressor tions as a ligand-dependent transcription factor. Although it is clear complexes have been shown to function with this heterodimer to that ecdysone elicits distinct transcriptional responses within its dif- regulate gene expression (5–8). -
Hoverflies Family: Syrphidae
Birmingham & Black Country SPECIES ATLAS SERIES Hoverflies Family: Syrphidae Andy Slater Produced by EcoRecord Introduction Hoverflies are members of the Syrphidae family in the very large insect order Diptera ('true flies'). There are around 283 species of hoverfly found in the British Isles, and 176 of these have been recorded in Birmingham and the Black Country. This atlas contains tetrad maps of all of the species recorded in our area based on records held on the EcoRecord database. The records cover the period up to the end of 2019. Myathropa florea Cover image: Chrysotoxum festivum All illustrations and photos by Andy Slater All maps contain Contains Ordnance Survey data © Crown Copyright and database right 2020 Hoverflies Hoverflies are amongst the most colourful and charismatic insects that you might spot in your garden. They truly can be considered the gardener’s fiend as not only are they important pollinators but the larva of many species also help to control aphids! Great places to spot hoverflies are in flowery meadows on flowers such as knapweed, buttercup, hogweed or yarrow or in gardens on plants such as Canadian goldenrod, hebe or buddleia. Quite a few species are instantly recognisable while the appearance of some other species might make you doubt that it is even a hoverfly… Mimicry Many hoverfly species are excellent mimics of bees and wasps, imitating not only their colouring, but also often their shape and behaviour. Sometimes they do this to fool the bees and wasps so they can enter their nests to lay their eggs. Most species however are probably trying to fool potential predators into thinking that they are a hazardous species with a sting or foul taste, even though they are in fact harmless and perfectly edible. -
Hoverfly (Diptera: Syrphidae) Richness and Abundance Vary with Forest Stand Heterogeneity: Preliminary Evidence from a Montane Beech Fir Forest
Eur. J. Entomol. 112(4): 755–769, 2015 doi: 10.14411/eje.2015.083 ISSN 1210-5759 (print), 1802-8829 (online) Hoverfly (Diptera: Syrphidae) richness and abundance vary with forest stand heterogeneity: Preliminary evidence from a montane beech fir forest LAURENT LARRIEU 1, 2, ALAIN CABANETTES 1 and JEAN-PIERRE SARTHOU 3, 4 1 INRA, UMR1201 DYNAFOR, Chemin de Borde Rouge, Auzeville Tolosane, CS 52627, F-31326 Castanet Tolosan Cedex, France; e-mails: [email protected]; [email protected] ² CNPF/ IDF, Antenne de Toulouse, 7 chemin de la Lacade, F-31320 Auzeville Tolosane, France 3 INRA, UMR 1248 AGIR, Chemin de Borde Rouge, Auzeville Tolosane, CS 52627, F-31326 Castanet Tolosan Cedex, France; e-mail: [email protected] 4 University of Toulouse, INP-ENSAT, Avenue de l’Agrobiopôle, F-31326 Castanet Tolosan, France Key words. Diptera, Syrphidae, Abies alba, deadwood, Fagus silvatica, functional diversity, tree-microhabitats, stand heterogeneity Abstract. Hoverflies (Diptera: Syrphidae) provide crucial ecological services and are increasingly used as bioindicators in environ- mental assessment studies. Information is available for a wide range of life history traits at the species level for most Syrphidae but little is recorded about the environmental requirements of forest hoverflies at the stand scale. The aim of this study was to explore whether the structural heterogeneity of a stand influences species richness or abundance of hoverflies in a montane beech-fir forest. We used the catches of Malaise traps set in 2004 and 2007 in three stands in the French Pyrenees, selected to represent a wide range of structural heterogeneity in terms of their vertical structure, tree diversity, deadwood and tree-microhabitats. -
New Records of Hover Flies (Diptera, Syrphidae) from Ukraine
Zoodiversity, 54(1):17–30, 2020 DOI 10.15407/zoo2020.01.017 UDC 595.773.1(477) NEW RECORDS OF HOVER FLIES (DIPTERA, SYRPHIDAE) FROM UKRAINE. IV A. V. Prokhorov1, G. V. Popov2, V. Yu. Shparyk3 1, 2Schmalhausen Institute of Zoology, NAS of Ukraine vul. B. Khmelnytskogo, 15, Kyiv, 01030 Ukraine 3Vasyl Stefanyk Precarpathian National University vul. T. Shevchenko, 57, Ivano-Frankivsk, 76018 Ukraine 1E-mail: [email protected] 2E-mail: [email protected] 3E-mail: [email protected] New Records of Hover fl ies (Diptera, Syrphidae) from Ukraine. IV. Prokhorov, A. V., Popov, G. V., Shparyk, V. Yu. — Six additional species of hover fl ies of the subfamily Eristalinae are recorded from Ukraine for the fi rst time: Criorhina pachymera Egger, 1858, Hammerschmidtia ferruginea (Fallén, 1817), Melanogaster parumplicata (Loew, 1840), Orthonevra erythrogona (Malm, 1863), Sphiximorpha garibaldii Rondani, 1860, and Temnostoma angustistriatum Krivosheina, 2002. Distributions of these species are summarized and species diagnoses are provided. Updated key to males of the European species of the genus Melanogaster including a little-known M. jaroslavensis (Stackelberg, 1922) is proposed. Key words: fl ower fl ies, Criorhina, Hammerschmidtia, Melanogaster, Orthonevra, Sphiximorpha, Temnostoma, new records, Ukraine. Introduction Hammerschmidtia Schummel, 1834 is the last addition to the list of hover fl y genera of Ukraine, currently with 83 genera. In Europe, this genus is represented by two species, one of which, H. ingrica Stackelberg, 1952, was recorded in Europe only from the northeastern parts (Finland and Russia). Th e second species, H. ferru- ginea (Fallén, 1817), is widespread in Europe (Speight, 2018), but rare and local in many habitats (Rotheray et al., 2008). -
Structure, Function, and Pharmaceutical Ligands of 5-Hydroxytryptamine 2B Receptor
pharmaceuticals Review Structure, Function, and Pharmaceutical Ligands of 5-Hydroxytryptamine 2B Receptor Qing Wang 1,2 , Yu Zhou 2 , Jianhui Huang 1 and Niu Huang 2,3,* 1 School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China; [email protected] (Q.W.); [email protected] (J.H.) 2 National Institute of Biological Sciences, No. 7 Science Park Road, Zhongguancun Life Science Park, Beijing 102206, China; [email protected] 3 Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University, Beijing 102206, China * Correspondence: [email protected]; Tel.: +86-10-80720645 Abstract: Since the first characterization of the 5-hydroxytryptamine 2B receptor (5-HT2BR) in 1992, significant progress has been made in 5-HT2BR research. Herein, we summarize the biological function, structure, and small-molecule pharmaceutical ligands of the 5-HT2BR. Emerging evidence has suggested that the 5-HT2BR is implicated in the regulation of the cardiovascular system, fibrosis disorders, cancer, the gastrointestinal (GI) tract, and the nervous system. Eight crystal complex structures of the 5-HT2BR bound with different ligands provided great insights into ligand recognition, activation mechanism, and biased signaling. Numerous 5-HT2BR antagonists have been discovered and developed, and several of them have advanced to clinical trials. It is expected that the novel 5-HT2BR antagonists with high potency and selectivity will lead to the development of first-in-class drugs in various therapeutic areas. Keywords: GPCR; 5-HT2BR; biased signaling; agonist; antagonist Citation: Wang, Q.; Zhou, Y.; Huang, J.; Huang, N. Structure, Function, and Pharmaceutical Ligands of 5-Hydroxytryptamine 2B Receptor. 1. Introduction Pharmaceuticals 2021, 14, 76. -
Compared Activity of Agonist Molecules Towards Ecdysone
PESTICIDES / SCIENTIFIC COMMUNICATION DOI: 10.1590/1808-1657000312019 Compared activity of agonist molecules towards ecdysone receptor in insect cell-based screening system Comparação da atividade de moléculas agonistas em relação ao receptor de ecdisona em um sistema de triagem baseado em linhagens celulares de insetos Ciro Pedro Guidotti Pinto1,2* , Letícia Neutzling Rickes2 , Moisés João Zotti2 , Anderson Dionei Grutzmacher2 ABSTRACT: The ecdysone receptor, naturally activated by ste- RESUMO: O receptor de ecdisona, naturalmente ativado por hor- roidal hormones, is a key protein for molting and reproduction mônios esteroidais, é uma proteína-chave nos processos de muda e processes of insects. Artificial activation of such receptor by specific reprodução de insetos. A ativação artificial desse receptor por meio de pesticides induces an anomalous process of ecdysis, causing death pesticidas específicos induz um processo de ecdise anômala, levando o of insects by desiccation and starvation. In this paper, we establi- inseto à morte por dessecação e inanição. Neste trabalho, foi estabele- shed a protocol for screening agonistic molecules towards ecdysone cido um protocolo para a triagem de moléculas agonistas em relação ao receptor of insect cells line S2 (Diptera) and Sf9 (Lepidoptera), receptor de ecdisona nas linhagens celulares responsivas S2 (Diptera) e transfected with the reporter plasmid ere.b.act.luc. Therefore, we Sf9 (Lepidoptera), transfectadas com o plasmídeo repórter ere.b.act.luc. set dose-response curves with the ecdysteroid 20-hydroxyecdysone, Para tanto, curvas de dose-resposta foram estabelecidas com o ecdiste- the phytoecdysteroid ponasterone-A, and tebufenozide, a pesticide roide 20-hidroxiecdisona, o fitoecdisteroide ponasterona-A e tebufeno- belonging to the class of diacylhydrazines. -
Ultraspiracle Promotes the Nuclear Localization of Ecdysteroid Receptor in Mammalian Cells
Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells By: Claudia Nieva, Tomasz Gwoóźdź, Joanna Dutko-Gwóźdź, Jörg Wiedenmann, Margarethe Spindler-Barth, Elżbieta Wieczorek, Jurek Dobrucki, Danuta Duś, Vince Henrich, Andrzej Ożyhar and Klaus-Dieter Spindler Nieva C, Gwozdz T, Dutko-Gwozdz J, Wiedenmann J, Spindler-Barth M, Wieczorek E, Dobrucki J, Dus D, Henrich V, Ozyhar A, Spindler KD. (2005) Ultraspiracle promotes the nuclear localization of ecdysteroid receptor in mammalian cells. Biol. Chem. 386:463-470. Made available courtesy of Walter de Gruyter: http://www.[publisherURL].com ***Reprinted with permission. No further reproduction is authorized without written permission from Walter de Gruyter. This version of the document is not the version of record. Figures and/or pictures may be missing from this format of the document.*** Abstract: The heterodimer consisting of ecdysteroid receptor (EcR) and ultraspiracle (USP), both of which are members of the nuclear receptor superfamily, is considered to be the functional ecdysteroid receptor. Here we analyzed the subcellular distribution of EcR and USP fused to fluorescent proteins. The experiments were carried out in mammalian COS-7, CHO-K1 and HeLa cells to facilitate investigation of the subcellular trafficking of EcR and USP in the absence of endogenous expression of these two receptors. The distribution of USP tagged with a yellow fluorescent protein (YFP-USP) was almost exclusively nuclear in all cell types analyzed. The nuclear localization remained constant for at least 1 day after the first visible signs of expression. In contrast, the intracellular distribution of EcR tagged with a yellow fluorescent protein (YFP-EcR) varied and was dependent on time and cell type, although YFP-EcR alone was also able to partially translocate into the nuclear compartment. -
Identification of Farnesoid X Receptor Β As a Novel Mammalian Nuclear Receptor Sensing Lanosterol
MOLECULAR AND CELLULAR BIOLOGY, Feb. 2003, p. 864–872 Vol. 23, No. 3 0270-7306/03/$08.00ϩ0 DOI: 10.1128/MCB.23.3.864–872.2003 Copyright © 2003, American Society for Microbiology. All Rights Reserved. Identification of Farnesoid X Receptor  as a Novel Mammalian Downloaded from Nuclear Receptor Sensing Lanosterol Kerstin Otte,1* Harald Kranz,1 Ingo Kober,1 Paul Thompson,1 Michael Hoefer,1 Bernhard Haubold,1 Bettina Remmel,1 Hartmut Voss,1 Carmen Kaiser,1 Michael Albers,1 Zaccharias Cheruvallath,1 David Jackson,1 Georg Casari,1 Manfred Koegl,1† Svante Pa¨a¨bo,2 Jan Mous,1 1 1 Claus Kremoser, † and Ulrich Deuschle † http://mcb.asm.org/ LION Bioscience AG, 69120 Heidelberg,1 and Max Planck Institute for Evolutionary Anthropology, 04103 Leipzig,2 Germany Received 19 September 2002/Returned for modification 31 October 2002/Accepted 12 November 2002 Nuclear receptors are ligand-modulated transcription factors. On the basis of the completed human genome sequence, this family was thought to contain 48 functional members. However, by mining human and mouse genomic sequences, we identified FXR as a novel family member. It is a functional receptor in mice, rats, rabbits, and dogs but constitutes a pseudogene in humans and primates. Murine FXR is widely coexpressed on March 22, 2016 by MAY PLANCK INSTITUTE FOR Evolutionary Anthropology with FXR in embryonic and adult tissues. It heterodimerizes with RXR␣ and stimulates transcription through specific DNA response elements upon addition of 9-cis-retinoic acid. Finally, we identified lanosterol as a candidate endogenous ligand that induces coactivator recruitment and transcriptional activation by mFXR.