University of Surabaya (UBAYA)
2ND INTERNATIONAL CONFERENCE ON NATURAL RESOURCES AND LIFE SCIENCES (NRLS 2018)
Managing Natural Resources for Better Health
Co-Organized by: Faculty of Biotechnology Faculty of Pharmacy Faculty of Medicine
University of Surabaya
IBIS STYLES HOTEL JEMURSARI, SURABAYA AUGUST 23-24, 2018
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Contents
Contents ...... 2 Chief Organizer’s Welcoming Speech ...... 3 Rector’s Opening Remarks ...... 5 NRLS 2018 Commitee ...... 7 PROGRAM SCHEDULE ...... 10 ORAL PRESENTATION SESSION ...... 12 POSTER SESSION ...... 17 INVITED SPEAKERS ...... 20 Prof. Intan Ahmad ...... 21 Erhan Simsek, Ph.D...... 22 Assoc. Prof. Dr. Kanyaratt Supaibulwatana ...... 23 Prof. Dr. Varaporn Junyaprasert ...... 24 Assoc.Prof. Dr.Heni Rachmawati ...... 26 Dr. Fazren Azmi ...... 27 Dr.rer.nat. Sulistyo Emantoko Dwi Putra ...... 28 Prof. Dr. Bob Wilffert ...... 29 ORAL ABSTRACTS ...... 30 POSTER ABSTRACTS ...... 75 MISCELANEOUS ...... 110 SPONSORS ...... 117
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Chief Organizer’s Welcoming Speech
The honorable Rector of UBAYA, Deans of UBAYA Faculties and faculty members, our eminent invited speakers, sponsors, ladies and gentlemen, a very good morning to you all!
As the times progressed, Biotechnology became evolved due to its wide scope of knowledge, or more easily called multidisciplinary. Many areas of science are biotechnology work areas, such as molecular biology, biochemistry, environmental biology, genetics, forensic biology, immunology, microbiology, pharmacology, botany, food technology and much more. It cannot be denied, each of these scholars have its own peculiarities, causing some difficulty of mixing up with each other. However, the combination of these disciplines is anticipated in order to bring more benefits to the audience than if the field of science is run independently.
Disruptive era is happening today, which is marked by a change in the existing global business order. Many established businesses are eroded by many businesses that are smaller because of the nature of sharing economy. Big retail uprooted because online stores began scattered. Big hotel uprooted due to business model like hospitality rental. It also may also have an impact on large industries that utilize natural resources, such as Monsanto or other major pharmaceutical industries. The use of natural resources whose information is more opened will be more welcome than the disclosed research results. Just imagine a science like Chinese traditional medicine, functional food, the use of markers with technology that can be distributed cheaply will explode in the market. This poses a challenge for scientists deepening the biological disciplines and natural resources to contribute to the wider community through their respective fields. The biotechnology community recognizes the need to work with others, such as experts from various fields such as pharmacy and medicine, to come up with ideas for health improvement efforts that can meet the needs of society and not just the science that dwells in the ivory tower. Therefore, the 2nd International Conference on Natural Resources and Life Sciences – NRLS 2018 this time theme “Managing Natural Resources for Better Health“.
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Coincide with it, the NRLS 2018 event also will be hosted for the Konsorsium Biologi Indonesia (KOBI) Congress-II and Ikatan Program Studi Bioteknologi Indonesia (IPSBI) Summit. It is likely that this event opens doors for collaborative opportunities for research and technology development among academics and professionals. Moreover, with the involvement of invited speakers from abroad, exchanges and allegedly research ideas with global trending can also occur.
On this occasion also, I want to inform the good news for all the colleagues, that Ubaya Biotech Store (UBS) has been initiated. UBS is a service center for biotechnology services and products which is a downstream part of research in the UBAYA Faculty of Biotechnology. Some products and services are still in the development stage, but to date, the item and service list is getting ready and increasing a lot to be too wasted if it is used only in the internal faculty. Going forward, we personally hope that will be a consortium of biotechnology services and products in the future, where relevant parties can exchange ideas in the development of biotechnology products and services and fulfill their needs.
We hope you will enjoy your time with us and being encouraged for the collaborations and friendship, scientific exchange, development of joint interests and project that are of scientific and economic importance in order to explore the natural resources and life sciences and their importance in the most aspect of living, e.g. food and health, agriculture, sustainable environment and renewable energy development.
Thank you for your time and have a nice day
Surabaya, August 23, 2018, Johan Sukweenadhi, Ph.D.
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Rector’s Opening Remarks
Good Morning, The NRLS Committee, Distinguished Invited Speakers, Ladies and Gentlemen, all friends from all overseas as well as from different places in Indonesia, It gives me great pleasure to extend to you all a very warm welcome to the University of Surabaya (UBAYA) Campus and to say how grateful we are that you have accepted our invitation to participate in this international conference held by the Faculty of Biotechnology, Faculty of Pharmacy and Faculty of Medicine UBAYA. I am so pleased to see many colleagues, professors, friends and families. We have here participants from seven countries, making our conference a global friendships celebration at once.
I would like to address my compliments to all committee and academic colleagues from UBAYA, who have been working a lot to prepare this event. Let me also address my gratitude also to industrial partners and sponsors participating in this conference. I also wish to specially welcome the representatives of biology and biotechnology related associations and express to you my deep appreciation for sharing this important moment.
After the implementation of 1st NRLS 2016 two years ago, this time the Faculty of Biotechnology works together with Faculty of Pharmacy and Faculty of Medicine, University of Surabaya in the implementation of second edition NRLS 2018. It is a great pleasure to me, knowing that three different faculties of Ubaya, can do such a wonderful collaboration to organize this scientific event beneficial to all parties. For your information, it is not the first time these three faculties work to collaborate with each other. Recently, at July 18th 2018, we inaugurated the new Laboratory named Kalbe Ubaya Hanbang-Bio Laboratory (abbreviated as KUH Laboratory), which was a collaboration project with the Industry. I hope the committee will have the opportunity to please you all taking a short visit to the KHU Lab at UBAYA Campus. All these events are actually part of our programs in celebrating the UBAYA 50Years Anniversary in 2018.
I have been informed that the theme of the today conference is “Managing Natural Resource for Better Health”. To my understanding, this is then the Conference where you can meet colleagues not only from your own specialty area, but you can also build connection to many colleagues from other fields for the sake of complementing each other towards a better scientific and technological achievements and applications in the area of Natural Resources and Life Sciences.
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I hope this conference provides you with a forum to exchange scientific ideas, inspire new research, and new contacts for closer co-operation, so that we can, together, envisage the future of a promising development of Natural Resources and Life Sciences to human’s benefits. Please carry also with you the best wishes of UBAYA for a productive and successful conference, and a pleasant stay amongst you in Surabaya.
Finally, on behalf of UBAYA, I am very proud to declare that the 2018 International Conference NRLS-2018 is officially opened. Thank you.
Surabaya, August 23, 2018, Prof. Ir. Joniarto Parung, MMBAT, Ph.D.
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NRLS 2018 Commitee
STEERING COMMITTEE Chairman : Nemuel Daniel Pah, S.T., M.Eng., Ph.D. (Vice Rector of Academic Affairs – UBAYA) Chief of Programme : Dr. rer. nat. Maria Goretti Marianti Purwanto (Dean of Biotechnology Faculty – UBAYA)
ORGANIZING COMMITTEE Chief of Executive : Johan Sukweenadhi, Ph.D. Vice of Chief Executive : Dr.rer.nat. Sulistyo Emantoko D. P.
1. Treasure . Dr. Ir. Popy Hartatie Hardjo, M.Si. . Riyadotul Husnah, SP.
2. Programme Section . Fenny Irawati, S.Si, M.Si . Wina Dian Savitri, S.Si., M.Agr. . Ardhia Deasy Rosita Dewi, S.TP., M.Sc. . Nina Dewi Oktaviyanti, S.Farm., M.Farm., Apt.
3. Administration Section . Olly Wicaksono Pratamasari, S.E., M.MT. . Nurul Azizah, S.Si. . Dwi Epatarini, S.Sos.
4. Scientific Committee . Dr. Dra. Mariana Wahjudi, M.Si. (Faculty of Biotechnology – University of Surabaya) . Dr.rer.nat. Sulistyo Emantoko D. P. (Faculty of Biotechnology – University of Surabaya) . Dr.rer.nat. Maria Goretti Marianti Purwanto (Faculty of Biotechnology – University of Surabaya) . Dr. Ir. Popy Hartatie Hardjo, M.Si. (Faculty of Biotechnology – University of Surabaya) . Johan Sukweenadhi, Ph.D. (Faculty of Biotechnology – University of Surabaya) . Prof. Ir. Lieke Riadi, Ph.D. (Faculty of Engineering – University of Surabaya)
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. Dr. Dra. R.R. Christina Avanti, M.Si., Apt. (Faculty of Pharmacy– University of Surabaya) . Dr. Finna Setiawan, S.Farm., M.Si. . dr. Irwin, M.Epid., M.Med.Ed. (Faculty of Medical– University of Surabaya) . dr. Risma Ikawaty, Ph.D. (Faculty of Medical– University of Surabaya) . dr. Nur Flora Nita Taruli Basa Sinaga, M.Kes., Sp.OT., M.H. (Faculty of Medical– University of Surabaya) . Prof. Intan Ahmad (Ministry of Research, Technology and Higher Education, Republic of Indonesia) . Prof. Dr. Bob Willfert (University of Groningen, Netherland) . Prof. Dr. Varaporn Junyaprasert (Mahidol University, Thailand) . Prof. Dr. Jagat R. Kanwar (Deakin University, Australia) . Dr. Fazren Azmi (Universiti Kebangsaan Malaysia, Malaysia) . Erhan Simsek, Ph.D. (Agilent Technologies Singapore) . Assoc. Prof. Dr. Kanyaratt Supaibulwatana (Mahidol University, Thailand) . Assoc.Prof. Dr. Heni Rachmawati (School of Pharmacy, Bandung Institute of Technology)
5. Promotion and Documentation Section . Mangihot T. Gultom, M.Sc. . Ida Bagus Made Artadana, S.Si., M.Sc.
6. Venue, Acommodation and Equipment Section . Dr. Dra. Tjandra Pantjajani, M.S. . Ruth Chrisnasari, S.TP., M.P.
7. Transportation Section . Ida Bagus Made Artadana, S.Si., M.Sc. . Fenny Irawati, S.Si., M.Si.
8. Sponsorship Section . Ernest Suryadjaja, S.Si., M.App.Sc.
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9. Workshop Section Ruth Chrisnasari, S.TP. Dr. Dra. Mariana Wahjudi, M.Si. Dr.rer.nat. Sulistyo Emantoko D. P. Akhmad Subhkan, S.Tp. Fitria Setya Megawati, S.Si. Ayu Mayzuhroh, S.TP.
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PROGRAM SCHEDULE
DAY I Thursday, August 23, 2018 Plenary Session 07.30-08.00 Registration 08.00-08.15 Traditional Dance UBAYA Dance Team Opening MC Singing: Indonesia National Anthem Biotechnology student 08.15-08.40 Welcome speech I Rector Welcome speech II Chief Organizer(s) Opening ceremony (gong) Keynotes Session I Moderator: Dr. rer. nat. Sulistyo Emantoko 08.40-09.15 Revisiting the capacity of Indonesia’s Prof. Intan Ahmad Higher Education in response to environment, energy and sustainability challenges 09.15-09.50 Solution to Discovery Metabolomics: High Erhan Simsek, Ph.D. Resolution Mass Spectrometry with Powerful Data Analysis Software 09.50-10.05 Discussion Moderator: Dr. rer. nat. Sulistyo Emantoko 10.05-10.20 Coffee break I Keynotes Session II Moderator: IBM Artadana, M.Sc. 10.20-10.55 Genetic interference and modified Assoc. Prof. Dr. Kanyaratt environments affected on growth and Supaibulwatana terpenoid variations of Artemisia annua L. 10.55-11.30 Role of Nanoparticles of Polymer-drug Prof. Dr. Varaporn Conjugate for Effective Drug Delivery Junyaprasert 11.30-11.45 Discussion Moderator: IBM Artadana, M.Sc. 11.45-12.00 Sponsor presentation I Sciencewerke 12.00-13.00 Lunch + Poster Session Parallel Session 13.00-14.00 Parallel session I 14.00-14.15 Coffee break II 14.15-15.15 Parallel session II
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DAY II Friday, August 24, 2018 Plenary Session 07.30-08.00 Registration 08.00-08.05 Opening MC 08.05-08.20 Traditional Dance Pharmacy students Keynotes Session I Moderator: Dr. Mariana Wahjudi 08.20-08.55 (Title) Prof. Dr. Jagat R. Kanwar 08.55-09.30 The pro-apoptotic effect of curcumin Assoc. Prof. Dr. Heni nanoemulsion versus curcumin solution in Rachmawati MCF-7 and MDA-MD cells in vitro 09.30-09.45 Discussion Moderator: Dr. Mariana Wahjudi 09.45-10.00 Coffee break I 10.00-10.10 Welcome speech Vice Rector 10.10-10.20 MoU Signing between UBAYA and other Biology/ Biotechnology Associations Keynotes Session II Moderator: Dr. Dra. R.R. Christina Avanti, M.Si., Apt. 10.20-10.55 Recent Advances in Nanotechnology-based Dr. Fazren Azmi Vaccines 10.55-11.30 Placental Global and Specific DNA Dr. rer. nat. Sulistyo Emantoko Methylation: A Mirror of Pregnancy and Birth Outcome Quality 11.30-11.45 Discussion Moderator: Dr. Dra. R.R. Christina Avanti, M.Si., Apt. 11.45-12.00 Sponsor presentation II Biotek Prima Indoplus 12.00-13.00 Lunch + Poster Session Parallel Session 13.00-14.00 Parallel Session I Keynotes Session III Moderator: Dr. Dra. R.R. Christina Avanti, M.Si., Apt. 14.00-14.35 Implementation of pharmacogenetics in Prof. Dr. Bob Willfert clinical practice 14.35-14.45 Discussion Moderator: Dr. Dra. R.R. Christina Avanti, M.Si., Apt. 14.45-15.00 Coffee break II Parallel Session 15.00-16.00 Parallel session II 15.00-16.15 Announcement of Best Poster and Best Oral MC Presenter 16.15-16.30 Closing MC
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ORAL PRESENTATION SESSION
DAY I Thursday, August 23, 2018
MEDICAL AND FORENSICS – BR LINE ROOM (OP-A-1 to OP-A- 6) CODE TITLE PRESENTER MODERATOR Quantification of Expression of Glucocorticoid Receptor of the Shintya OP-A-1 Offspring of Mus musculus BALB/c of Stressed Parents: Folic Tanggara Acid Intervention Study Understanding the Current Event Mariana OP-A-2 Risma of Tuberculosis in Indonesia : Wahyudi Challenges and Opportunities Ikawaty Anticancer Potential from Ethanol Extract of Zanthoxylum Emilia Vivi OP-A-3 acanthopodium Seed to Against Arsita MCF-7 Cell Line DISCUSSION COFFEE BREAK Biological Screening of Leaf and Fruit Extract (Ficuscarica L) Risa OP-A-4 using Brine Shrimp Lethality Test (BSLT) Toxicity Bioassay on Purnamasari Arthemia salina Leach Larvae Designing of a Less- Sulistyo Immunogenic Nattokinase from Yoanes Maria Emantoko OP-A-5 Bacillus subtilis subsp. natto: A Vianney Computational Mutagenesis
Alpha Mangostin Effect on MDA Level and The Pancreatic Devyana Dyah OP-A-6 Morphology Rattus novergicus Wulandari Induced Alloxan DISCUSSION
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AGRICULTURE – BR STRIPES ROOM (OP-B-1 to OP-B-10) CODE TITLE PRESENTER MODERATOR Plant Regeneration Through Somatic Embryogenesis from Popy Hartatie OP-B-1 Callus of Vanda tricolor Hardjo Lindl. var. pallid Influence of Types and Concentration of Natural Dyah OP-B-2 Pesticides to Mortality of Red Mite (Panonychus citri Roeswitawati McGregor) Effect of Salinity on Biomass Allocation and Whole Plant Johan Mass of Rice (Oryza sativa Sukweenadhi OP-B-3 Niki Habibi L) Related to Growth at Panicle Initiation and Heading Stages Study of Ccs and pAMT Gene of Genotype 1 Cayenne Nur OP-B-4 Pepper (Capsicum frutescens Rahmattullah L.) Original Type and Mutant Menthol from Stem and Leaf Bintang OP-B-5 in vitro Mentha piperita Kerta Wijaya DISCUSSION COFFEE BREAK Analysis of Abscisic Acid, Salicylic Acid and GABA in OP-B-6 Susy Saadah Several Oil Palm Progenies in Drought Stress Uptake of Macro Nutrients OP-B-7 and Growth of Mahang Dwi Susanto plants (Macaranga gigantea) The Evaluation of Breeding Value and Most Probable Producing Ability for the Popy Hartatie OP-B-8 Aris Winaya Basis Selection of Ettawa Hardjo Croosbred Goat at Malang Region, East Java, Indonesia InVitro Multiplication Of Fig Ivon Grace OP-B-9 (Ficus carica L.) for Paulin Commercially Industry Mild Alkaline Pretreatment on Sugarcane Bagasse: OP-B-10 Lieke Riadi Effects of Time and Lime Ratio to Dry Bagasse DISCUSSION
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FOOD AND NUTRITION – BR CURVE ROOM (OP-D-1 to OP-D-10) CODE TITLE PRESENTER MODERATOR Effect of Phosphoric Acid Pretreatment on Characterization OP-D-1 Dewi Yuliani of Gelatin from Broiler Chicken Bones Molecular Characterization of Potentially Probiotic Bacterium Yulia Sari OP-D-2 Isolate from Mackerel Fish Ismail (Rastrelliger sp.) Sulistyo Profilling Of Shrimp Shell
OP-D-3 Hydrolysis Product By Chitinase Maria Goretti Emantoko From Bacillus licheniformis B2
Production, Purification and Adelia Angelin OP-D-4 Characterization of Chitinase from Bacillus licheniformis D11 Anastasia Purification and Characterization Maria Fidelia OP-D-5 Of Chitinase from Paenibacillus sp. Susanto DISCUSSION COFFEE BREAK
Rapid Reversed-Phase High Performance Liquid OP-D-6 Chromatography Assay of tert- Steven Sutanto Butylhydroquinone content in Food Products Characterization of OP-D-7 Thermostable Chitinase from Nalista Jayanthi Bacillus licheniformis B2 The Isolation and Screening of Proteolytic Bacteria from Ardhia Deasy Sidoarjo Shrimp Paste as OP-D-8 Evi Susanti Protease Source to Extract the R.D. Collagen Protein of Milkfish Scales A New Specific Primer for Detection of Pig genome by Mariana OP-D-9 Quantitative Polymerase Chain Wahjudi Reaction The Method of Growing Tubular Acetobacter xylinum on the OP-D-10 Salafudin Solid Media as Precursor of Sausage Casing DISCUSSION
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DAY II Friday, 24August 2018
MEDICAL AND FORENSICS – BR STRIPES ROOM (OP-A-7 to OP-A-14) CODE TITLE PRESENTER MODERATOR In Vivo Efficiency of Collagen Extract from Sangkuriang Catfish (Clarias gariepinus var. OP-A-7 Andini Ary Sangkuriang) Skin on MDA Levels and Macrophages Total in Burn Wound Healing The Effect Of Folic Acid Supplementation In Parental OP-A-8 Stress Mouse Toward NR3C1 Patricia Mariana
Expression Alteration In The Wahyudi Offspring In Silico Mutagenesis: Decreasing the Stanley Evander OP-A-9 Immunogenicity of Botulinum Emeltan Tjoa toxin Type A Comparison of food intake OP-A-10 containing vitamin D with BMI Rivan Virlando of Geriatric patients in Suryadinata Sidoarjo, East Java DISCUSSION COFFEE BREAK Influenceof Folic Acid Diet on OP-A-11 Maternal Stress to Offspring Natanya Stress Level and NR3C1 Expression Facile Green Synthesis of Silver Nanoparticles from Sukweenadhi OP-A-12 Ethanol Extract of Plantago
major L. and its antibacterial Johan Sulistyo activity Emantoko In Vitro Evaluation of Chitosan-DNA Plasmid Asmarani OP-A-13 Complex Encoding Jembrana Kusumawati Disease Virus Tat Protein as a Vaccine Candidate The Relationship between Asthma Control - OP-A-14 Methylxanthine used and Mariana Wahjudi cyp1a2*1f Gene Polymorphisms
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DISCUSSION
ENVIRONMENT & ENERGY(1) – BR CURVE ROOM (OP-C-1 to OP-C-6) CODE TITLE PRESENTER MODERATOR Utilization Cytochrome B Gene for Phylogeny Analysis of Dwi Martha OP-C-1 Cuora amboinensis to Prevent Nur Aditya Extinction: Preliminary Study Profile of Antibiotic Compounds Mangihot Tua OP-C-2 fromTrichodermaharzianumin IBG Darmayasa Goeltom Controlling Aspergillus flavusonBaliLontar Plasmid Curing Analysis of OP-C-3 Chromate Reducing Bacteria Badruzsaufari Isolated from Serpentine Soil DISCUSSION COFFEE BREAK Effect of Cycle Time on Biokinetics Parameters in OP-C-4 Tapioca Wastewater Treatment Happy Mulyani using Sequencing Batch Reactor The Utilization of Tofu Waste Finna OP-C-5 for the Cultivation of Spirulina Yalun Arifin for Biomass Production Thermostable Chitinase- Producing Bacterium from Ijen Ruth OP-C-6 Hot Spring – Indonesia: Isolation, Identification and Chrisnasari Characterization DISCUSSION
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POSTER SESSION SCHEDULE
Thursday – Friday, 23 – 24 August 2018 Prefunction Area
MEDICAL AND FORENSICS (PP-A-1 to PP-A-4) CODE TITLE PRESENTER PP-A-1 Antibacterial Activity Roll on Deodorant With Oom Komala Plucheaindica (L.) Leaf Extract Against Staphylococcus epidermidis in-Vitro
PP-A-2 Formulation of Padinaaustralis Extract Tablet as Tri Saptari Antibacterial Escherichia coli Haryani PP-A-3 The Potential of Pacitan Orange (Citrus sinensis) Rinza Peel Extract as a Stimulus of Regeneration Cells in Rahmawati Rattus norvegicus Burns Samsudin PP-A-4 Characterization and Antibacterial Activity Test of Simbion Sponge Bacteria Against Pathogenic Endang Bacteria
AGRICULTURE (PP-B-1 to PP-B-5) CODE TITLE PRESENTER PP-B-1 Genetic Variability of Soybean Genotypes for Pod Ayda Shattering Resistance using Oven Methods Krisnawati PP-B-2 Characterization of Adaptive and Productive M Muchlish Soybean Genotypes in Dry Land of Kalimantan, Adie Indonesia PP-B-3 The Performance of Three Local Rice (Oryza sativa) Ida Bagus Made Cultivar from East Kalimantan under Osmotic Stress Artadana at Early Seedling Stage
PP-B-4 Production Potential of Sweet Corn (Zea mays Linn. P G Adi Nurani Var. 17accharate) to Different Planting Pattern and P Sources
PP-B-5 Local Variety of “Uwi” (Dioscorea alata Linn.) Wuryanto Plant in West Region of East Java Upland
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ENVIRONMENT & ENERGY (PP-C-1 to PP-C-7) CODE TITLE PRESENTER PP-C-1 Phytotoxicity and Developmental Effect of Wuye Ria CASHEW Nut Shell Extract against Nymphal Andayanie Instar of Whitefly (Bemisia tabaci Genn.) PP-C-2 Applying Species Distribution Models to Cicik Suriani Identify the Conservation Area by Area Habitat Suitability: A case study using Zanthoxyluma canthopodium DC. PP-C-3 An Engineering of Floating Net Aquaculture D Rachmawati System of Tiger Grouper [Ephinephelus fuscoguttatus (Forsskal, 1775)] on suplementation Bacillus subtilis (Ehrenberg, 1835) Probiotic in the Diet PP-C-4 Performance UNEJ #1 Stove Low Energy using S S Harsono Bio-pellet from Coffee Husk PP-C-5 Fish Diversity in Cimanuk River Before and T Herawati After Rainy Season in Jatigede reservoir, West Java, Indonesia PP-C-6 The Fe Non-heme Prospect in Coffee Flour made RHSetiabudi from Coffee Solid Waste: Mini review PP-C-7 Does the utilization of waste as the green energy N Syaifudin alternative economically feasible?
FOOD AND NUTRITION (PP-D-1 to PP-D-10) CODE TITLE PRESENTER PP-D-1 Preparation and Characterization of Butterfly Pea Hana Susanti Color Extract Encapsulated with Various Maleta Coating Agents PP-D-2 Photodynamic Inactivation of Escherichia coli Renny Indrawati by the Crude Pigments Extract of Various Tea Dregs PP-D-3 The Function of Ethanolic Extract Plectranthus Melva Silitonga ambioinicus Lour Spreng as Preventive and Curative Against The Effects of Rhodamine B in Rats
PP-D-4 Potency of Vegetable Oil to Increase Microflora Nurhayati 18 International Conference of Natural Resources and Life Sciences 2018
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Health Using In Vitro and In Vivo Evaluation PP-D-5 Quality and Consumer Acceptance Improvement Elfi Anis Saati on Prime Local Soy Milk (Glycine max (L) Merrill) Varieties PP-D-6 The Utilization of Telang Flower as Healthy- Elfi Anis Saati Natural Food Coloring on Dawet Drink PP-D-7 Artificial Rice as an Alternative Functional Food Erica Valencia to Support Food Diversification Program PP-D-8 Critical Aspects to Produce Low-Cost Protein Leonardo Tejo Molecular Weight Marker Gunawan PP-D-9 Potency healthy Moringa oleifera (Lamk) leaf S.S Harsono flour as home-agroindustry in rural area of Eastren East Java - Indonesia PP-D-10 Quality of Milk Pasteurization of Ginger in Cold KK Khotimah Strorage
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INVITED SPEAKERS
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Revisiting the capacity of Indonesia’s Higher Education in response to environment, energy and sustainability challenges
Intan Ahmad
Ministry of Research, Technology and Higher Education, Republic of Indonesia [email protected], [email protected]
The 21st century has witnessed increasing environmental, energy and sustainability challenges in our lives that need to be addressed by our Higher Education system. On one hand, Indonesia’s current predicaments in fulfilling Sustainability Development Goals (SDGs) have invited international scrutiny, particularly with the rapid depletion of Indonesia’s natural resources. On the other hand, whilst many Indonesian universities have managed to put these challenges in their agenda and produced relevant innovations and research to solve the recurring problems, we are still lagging behind compared to other countries in Asia, both in terms of publications and intellectual property rights related to environment and energy. This speech highlights some of the strategies we need to look into to address environment, energy and sustainability challenges and stand equally with international scholars in providing globally-oriented solutions and insights. This, as I argue, can be achieved by revisiting the potentials, roles and responsibilities of Indonesia’s Higher Education system.
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Solution to Discovery Metabolomics: High Resolution Mass Spectrometry with Powerful Data Analysis Software
Erhan Simsek
Agilent Technologies Singapore [email protected]
The field of metabolomics has seen tremendous growth in recent years with important applications in basic and life sciences. Mass spectrometry coupled to chromatographic separation techniques is one of the major analytical platforms to analyze metabolites in complex biological samples due to its highly sensitive and fast data acquisition capabilities. This presentation will cover workflows and instrumentation for discovery as well as targeted metabolomics approaches. Data analysis steps such as feature extraction, statistical analysis, metabolite identification and pathway analysis will be covered with relevant software tools such as Profinder, Mass Profiler Professional and Pathway Architect.
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Genetic Interference and Modified Environments Affected on Growth and Terpenoid Variations of Artemisia annua L.
Kanyaratt Supaibulwatana
Department of Biotechnology, Faculty of Science, Mahidol University, Bangkok, Thailand [email protected], +66 (0)2 2201 5303
Abstract Plant secondary metabolites are the final products of regulatory processes and the level of metabolite can be influenced depending on genetic or environmental changes. Among plant secondary metabolites, terpenoids are a most structural diverse group formed from 5-C subunits, isoprene, synthesized from movalonate and 1-deoxy-D-xylulose-5-phosphate (DOXP) pathway. In recent years, incorporation of biotechnology with the advance technologies of analytical biochemistry and high-throughput omics led to more increasing numbers of complete plant genome sequences and better understanding in functional genomic study and more importantly, their role as a basis for drug development. Besides the genetic interferences by metabolic bioengineering, induced mutation are also useful to establish new mutants that may be implemented for production of high-yielding of high-value phytochemical. In this presentation, genetic interferences incorporated with modified environmental controlled environments with artificial lights that had effects on morphological growth and phytochemical changes will be demonstrated using a medicinal herb, Artemisia annua L. as a model plant. A. annua L., a medicinal herb belongs to family Asteraceae. It is so-called as “Qinghao” in Chinese that has been the used as anti-fever and anti-malarial drugs for more than thousand years. The phytochemical investigation of this plant revealed that a sesquiterpene lactone compound named artemisinin has been predominantly detected in the aerial parts of this plant, particular in the leaves. The variations of plant growth, terpenoids production and its bioactivity affected by genetic manipulation and external stimulators will be discussed.
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Role of Nanoparticles of Polymer-drug Conjugate for Effective Drug Delivery
Varaporn Junyaprasert
Department of Pharmacy, Faculty of Pharmacy, 2Center of Excellence in Innovative Drug Delivery and Nanomedicine, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand [email protected]
One of the most useful techniques to modify and control drug release or to achieve targeted delivery is to use polymer-drug conjugated nanoparticles. Polymer-drug conjugates are technologies in which a drug is covalently bound to a polymeric carrier, normally via a biodegradable linker. The main benefits of polymer-drug conjugates are improvement of properties of drug carriers, leading to polymer carriers with high loading capacity and enhanced cellular uptake. In addition, grafting the drug onto the polymer backbone can modify the drug release and reduce burst drug release. Targeted delivery is generally required for toxic drug substances e.g. anticancer and antineoplastic drugs so as to enhance the specific delivery to a particular organ for maximum effect, thereby reduce or eliminate unwanted side effects to healthy tissues. The polymer-drug conjugated based nanoparticles possess ability to deliver the drug to targeted cells or organs by passive targeting via the enhanced permeability and retention (EPR)effect and active targeting by functionalizing targeting ligands onto the nanoparticle surface (2-3). Therefore, the polymer- drug conjugates have demonstrated favorable physicochemical properties to enhance drugdelivery efficiencyIn this lecture, two studies of polymer-drug conjugates have been demonstrated to use as drug targetingand delivery. The first study is “Enhanced toxicity and cellular uptake of methotrexate (MTX)- conjugatednanoparticles in cancer cells by decorating with folic acid- conjugated d-α-tocopheryl polyethylene glycol1000 succinate (TPGS-FOL)” (4). The second one refers to “Cationized triblock and diblock poly( - caprolactone)-copoly(ethylene glycol) (PCL-PEG) copolymers for oral delivery of enoxaparin (ENX)” (5-6). In the first study, the results confirmed the successful synthesis of TPGS-FOL. In addition, the decoration of TPGS- FOL onto the MTX nanoparticles slightly enlarged the size and enhanced cytotoxicity and uptake efficiency to MCF-7. Furthermore, 10% MTX- conjugated nanoparticles and FOL-decorated nanoparticles showed higher %uptake, as compared to 20% MTX-conjugated nanoparticles and undecorated 24 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
nanoparticles. For the cationized PCL-PEG copolymers for ENX delivery, the study showed that the content of PTA on P(CL) chain had a large impact on the copolymer and particle properties, the cytotoxicityof Caco-2 cells, drug encapsulation, and release behavior. However, the different conformations of the ENX-P(CatCLCL)2-PEG and ENX-P(CatCLCL)-mPEG particles did not affect the particle properties, cellular uptake, and permeation. These results suggested that these novel cationized copolymers would be potential carriers for oral delivery of ENX.
International Conference of Natural Resources and Life Sciences 2016 25
University of Surabaya (UBAYA)
The Pro-Apoptotic Effect of Curcumin Nanoemulsion versus Curcumin Solution in MCF-7 and MDA-MD Cells In Vitro
Heni Rachmawati1,2, Najwa Nabila1, Christian1, Yogi Handoko1, Sabina Quarder3, Kazunori Kataoka3
1School of Pharmacy, Bandung Institute of Technology, Ganesha 10 Bandung 40132, Indonesia 2Research Center for Nanosciences and Nanotechnology, Bandung Institute of Technology, Ganesha 10 Bandung 40132, Indonesia 3Innovation Center of NanoMedicine, 3-25-14 Tono-machi, Kawasaki-ku, Kawasaki City, Kanagawa Prefecture, Japan Heni Rachmawati ([email protected])
Curcumin, a main and important active compound present in Curcuma sp., demonstrates a wide range of positive biological effects including for cancer. However, due to low bioavailability caused by low solubility and stability as well as intensive hepatic metabolism, the clinical outcome during curcumin therapy is unsatisfied. Hence, we established nanocarrier system to improve the lack of curcumin which was confirmed to be successful for our various studies. In this report, we describe the potential use of curcumin loaded nanoemulsion for anticancer. We performed uptake study of curcumin-loaded nanoemulsion compared with curcumin solution in various cancerous cell models including MCF-7, MDA-MB 231, Tov-21, and HepG2 cells. Among cell models tested for in vitro study, MCF-7 cell showed the most sensitive cell type to curcumin indicated by lowest IC50 value. Negative charge of curcumin-loaded nanoemulsion was failure to cross the cell membrane of all cell lines. However, slightly positive charge of curcumin-loaded nanoemulsion exhibited better penetration to reach the cell nuclei shown by the green spots surrounding the cell nuclei, although less as compared to curcumin solution. The capability of curcumin to cross the cell membrane was followed by the apoptotic effect on both MCF-7 and MDA-MB 231 cells, which was concentration dependent. According to this finding, we suggest one of anticancer activity of curcumin is through apoptotic pathway which is also reported by other groups. Our established nanocarrier system might improve this effect via enhancing the pharmaceutic properties of the curcumin.
26 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
Recent Advances in Nanotechnology-based Peptide Vaccines
Fazren Azmi
Faculty of Pharmacy, National University of Malaysia [email protected]
Vaccination has emerged as the most effective and economically viable medical discovery to improve public health. Majority of the conventional vaccines were produced using live or attenuated microorganisms or parts of them. However, the use of whole organisms or large proteins has several weaknesses, such as the presence of biological impurities and unnecessary epitopes which may contribute to reactogenic responses. Peptide vaccines offer an alternative strategy as it contains specific epitope of an antigen, thus eliminate the allergenic/reactogenic sequences. Interestingly, the use of peptides as antigen open a new concept in vaccination, where there is a renewed interest in developing therapeutic vaccine for certain chronic diseases, such as cancer. However, peptide antigens produce a relatively weak immune response which necessitate the need of immunostimulants (adjuvants) in vaccine formulation for optimal efficacy. The use of nanoparticles in peptide vaccine formulations can boost the antigen effectiveness and can be functionalised for a targeted delivery. Various types of nanoparticles such as virus-like particles, liposomes and polymeric nanospheres have been developed as potential delivery vehicles for peptide-based vaccine antigens. Recent approach in utilising theself-assembling peptides as a nanocarrier platform for peptide vaccines will be discussed in detail.
International Conference of Natural Resources and Life Sciences 2016 27
University of Surabaya (UBAYA)
Placental Global and Specific DNA Methylation: A Mirror of Pregnancy and Birth Outcome Quality
Sulistyo Emantoko
Department of Biology, Faculty of Biotechnology, University of Surabaya Jalan Raya Kalirungkut Surabaya 60292, East Java Province, Indonesia [email protected]
Fetal programming refers to epigenetic adaptative measures to adverse prenatal environments which affect the birth outcome and disease susceptibility in later life. Amongst other epigenetic mechanisms, DNA methylation plays an important role in fetal programming. DNA methylation dynamics during embryogenesis, development, and baby born are important windows in the mammalian life cycle. DNA methylation is possible to measure at a global level as represent the amount of 5mC in the genome or at a specific level of the certain gene. Placenta as important organ which developed during this time period, experience the same DNA methylation dynamics. At the end of this process, a specific DNA methylation pattern is figured out as a response to its surroundings. A complex interaction between mother, placenta, and child ensures an ideal supply of nutrients, oxygen and endocrine signals, all necessary requirements for normal development. This prenatal environment strongly depends on maternal health and placental function. Maternal pregnancy-related diseases like gestational diabetes (GDM) are known to disturb placental function and the prenatal environment. As such, maternal diseases can impact on birth outcomes and according to the theory of fetal programming, increase the risk for disease in later life of the newborn.
28 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
Implementation of Pharmacogenetics in Clinical Practice
Prof. Dr. Bob Wilffert
Department of Pharmaco Therapy, -Epidemiology and –Economics, Faculty of Science and Engineering, University of Groningen, Netherland [email protected]
Pharmacogenetics is one source for explaining inter individual differences in drug response, besides factors like age, ethnicity, organ dysfunction, disease, pregnancy, gender, etc. The value of the application of pharmacogenetics in pharmacokinetics (as example: CYP2D6, CYP2C19, CYP2C9), pharmacodynamics (as example: VKORC1) and side effects (as example: HLA-B*5107) will be described. The effect of pharmacogenetic variation in CYP2D6 on the bioavailability of oral nortriptyline will be compared with the requirements for bioequivalence. There are big, drug specific differences in the role of pharmacogenetics and its clinical impact. The role of ethnicity in pharmacogenetics will also be illustrated. The initiative of the Pharmacogenetics Working Group of the Royal Dutch Pharmacists Association taken in 2005 to facilitate implementation of pharmacogenetics in clinical practice will be presented. This working group consists of (clinical) pharmacists, physicians, clinical chemists, epidemiologists and clinical pharmacologists. Literature is searched for pharmacogenetic information. The way of assessing level of evidence and of defining the clinical implications will be explained, as well as the translation into clinical practice. A scoring system for the necessity of preemptive genotyping will be presented. The advices formulated by the working group are also taken up on the website from PharmGkb. Pharmacogenetics can be applied to select the most appropriate drug for the individual patient and should be applied more in clinical practice.
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University of Surabaya (UBAYA)
ORAL PRESENTER
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University of Surabaya (UBAYA)
MEDICAL & FORENSICS
International Conference of Natural Resources and Life Sciences 2016 31
University of Surabaya (UBAYA)
OP-A-1 QUANTIFICATION OF GLUCOCORTICOID RECEPTOR EXPRESSION IN Mus musculus BALB/c OPFFSPRING OF STRESSED PARENTS: FOLIC ACID INTERVENTION STUDY
Shintya Tanggara, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract HPA axis plays a crucial role in regulating stress mechanism and is made up of complex system in support of that. One of the components that plays a major in stress management is the glucocorticoid receptor (GR) expressed throughout several areas of the body and especially hippocampus. GR is responsible in regulating cortisol level in the body and is encoded by the gene NR3C1. Previous studies shown the plasticity of hippocampus along with NR3C1’s susceptibility to epigenetic changes. The possiblility of chronic restraint stress (CRS) on both male and female BALB/c mice to induce epigenetic reprogramming is evaluated using forced swim test (FST) as behavioural test along with intensity ratio of NR3C1:GAPDH as molecular test. Effect of folic acid on both parents was also investigated as it has been shown to have antidepressant propertie and can normalize epiegenetic patterns in previous studies. Behavioural test show for parents show that there is a significant difference in the result, which is p=0.028 of CRS induced parents, offspring of CRS induced parents (p=0.014), and normal parents. There is no significant difference on behavioral test for folic acid-CRS (p=0.071) and folic acid- normal (p=0.455) parents and for offspring as well (p=0.868 and p=0.890 respectively). Molecular result of the experiment was done on offspring and showed significant difference on offpring with stressed parents (p=0.043) and no significant difference on normal-folate offspring (p=0.833) and stress-folic acid offspring (p=0.631).
Keywords: BALB/c mice, chronic restraint stress (CRS), epigenetics, forced swim test (FST), gene expression quantification, glucocorticoid receptor (GR), HPA axis, NR3C1
32 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-2 UNDERSTANDING THE CURRENT EVENT OF TUBERCULOSIS IN INDONESIA: Challenges and Opportunities
Risma Ikawaty, M.D., Ph.D.
Medical Faculty, University of Surabaya, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract Multi-drug resistant Mycobacterium tuberculosis (MDR-TB) is a growing international health problem, and it is mostly concentrated in developing countries. In 2016, the World Health Organization reported that Indonesia became the second largest contributor of global tuberculosis (TB) just after India. The estimated incidence of TB burden was 1.020.000 of TB with HIV and 32.000 of MDR/RR-TB (MDR is TB resistant to rifampicin and isoniazid, while RR is rifampicin resistant). Increasing prevalence of MDR-TB strains combined with HIV co-infection contributed greatly to the difficulties of TB control and treatment. Epidemiological evidence of the rising MDR-TB threat including mortality rate of 42/100.000 population, total new case and relapse around 364.671, and TB treatment coverage was about 36% among 261 million population in Indonesia. In this study we will review a comprehensive aspect of epidemiologic triangle of MDR-TB in Indonesia. As we know that long-life battle between MTB and the human host is determined by complex of host- pathogen-environmental factors. Rifampicin as the first line anti-TB drug is metabolized through hepatic cytochrome P450 enzyme system. Pharmacogenomics analysis ofCYP450 genes provides the information whether the patients are poor-, intermediate-, extensive-, or ultra-rapid metabolizer, which further shows significant variability in drug responses including optimal dose,efficacy and adverse drug reactions.At the same time, an extensive whole- genome studies reveal various molecular targets related to resistance mechanisms in MDR-TB(i.e.rpoB, rpoA, rpoC, gyrA, gyrB, etc) including cross-resistance, resistance pathways, and yet the spontaneous mutations in drug targets ensure the survival of M. tuberculosis.Environment factors also play an important role to the TB treatment success rate. Several contributing factors such as tobacco use (i.e. smoking), nutritional status, poor patient- medication adherence, poor drug quality/supply, socio- economic status, etc. These overall facts has made the approach and treatment of tuberculosis become even more challenging, and further the development of novel and efficient therapeutic substances is needed.
International Conference of Natural Resources and Life Sciences 2016 33
University of Surabaya (UBAYA)
Keywords: Multi-drug resistant Mycobacterium tuberculosis, epidemiologic triangle, pharmacogenomics, whole-genome study
34 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-3 Anticancer Potential from Ethanol Extract of Zanthoxylum acanthopodium Seed to Against MCF-7 Cell Line
Emilia Vivi Arsita*1, Dora Erawati Saragih1, Kelvin Aldrin2
1 Padjadjaran University, Faculty of Math and Science, Bandung/Sumedang Road, West Java 45363, Indonesia 2 Padjadjaran University, Faculty of Pharmacy, Bandung/Sumedang Road, West Java 45363, Indonesia *Corresponding author: [email protected]
Abstract Andaliman (Zanthoxyluma canthopodium DC) is a wild plant species typical of North Sumatera that often used as spice for Batak cuisine. Studies conducted by Wijaya (1999) show that Andaliman has potential as anticancer drug. Based on statistic data, breast cancer is type of cancer that most common in women. Cell MCF-7 is one of breast cancer model. The purpose of this research was to identified types of secondary metabolites which found in andaliman’s seed extract and to test the ability of these compound in stopping the MCF-7 cell activity. The method in this research is extraction use ethanol with different concentration and MTT Assay. Identified secondary metabolites such as phenol, saponins, flavonoids, tannins, terpenes, steroids, and alkaloids. IC50 MTT Assay value with absorbance of 550 nm was 221.31 ppm. IC50 values indicate that the andaliman seed ethanol extract has potential as an anticancer and has an effect quite actively on inhibition of MCF-7 cancer cell proliferation as well as having a strong effectiveness on pure extract.
Keywords: Andaliman, anticancer, breast cancer, MCF-7, MTT Assay, secondary metabolites
International Conference of Natural Resources and Life Sciences 2016 35
University of Surabaya (UBAYA)
OP-A-4 Biological Screening of Leaf and Fruit Extract (Ficus carica L) using Brine Shrimp Lethality Test (BSLT) toxicity bioassay on Arthemia salina Leach Larvae
Risa Purnamasari*1, Eva Agustina1, Nova Lusiana2, Miftakhur Rohmah1
1 Sains and Tekhnologi Faculty, UIN Sunan Ampel Surabaya, Indonesia. 2Psikologi and Health Faculty, UIN Sunan Ampel Surabaya, Indonesia. *Corresponding author: [email protected]
Abstract Brine Shrimp Lethality Test is the most convenient system for monitoring biological activities of various plant species. This method is very useful for preliminary assessment of toxicity of the plant extracts, it`s also used as a preliminary test against anti cancer activity by looking at the death of larvae caused by active compounds contained in the extract. This study aims to determine the effects of the cytotoxicity of leaf and fruit methanol extract Ficus carica L. using Brine Shrimp Lethality Test (BSLT) method. Ficus carica L. Leaves and fruit are extracted by maceration method using methanol solvent. However, some conditions have to be resolved, especially in standard experimental conditions such as temperature, medium pH, salinity, and aeration. Furthermore, toxicity test using shrimp larvae Artemia salina Leach. Ficus carica L. extracts using this test have been determined in the concentration range of 10, 100, 200, 500 and 1000 ppm of the examined Ficus carica L. extract. Toxic effects of leaf and fruit extract can be seen between the percentage of larval mortality and log concentration to obtain LC50. leaf and fruits extract are toxic with values of each LC50 are 44.66 and 45.76. This is because of compounds such as flavonoids, Triterpenoids and Sterols, Alkaloids and Saponins contained in leaf extracts and fruits of Ficus carica L.
Keywords: cytotoxicity, Brine Shrimp Lethality Test, Ficus carica L.
36 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-5 Designing of a less-immunogenic Nattokinase from Bacillus subtilis subsp. natto: a computational mutagenesis
Yoanes Maria Vianney*, Stanley Evander Emeltan Tjoa, Sulistyo Emantoko Dwi Putra
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract Nattokinase is an enzyme produced by Bacillus subtilis subsp. natto that contain strong fibrinolytic activity. It has potential to treat cardiovascular diseases. In-silico analysis revealed that nattokinase is considered an antigen, thus hindering its’ application for injectable therapeutic protein, in addition to this protein is produced by bacteria and foreign to human. Various web servers were used to predict B-cell epitopes of nattokinase both continuously and discontinuously to determine which amino acid residues is responsible for the immunogenicity. With the exclusion of the predicted conserve amino acids, four amino acids were allowed to be mutated, which were S18, Q19, T242, and Q245. Substitution mutation was done to lower the immunogenicity of native nattokinase. Considering the stability of the mutated protein with the help of Gibbs’ free energy difference, the proposed mutein was S18D, Q19I, T242Y and Q245W. 3D validation, stability analysis of the protein, potential energy measurement of molecular docking, and RMSF determination from molecular dynamics analysis, the mutein displayed lower antigenicity and does not differ from the native protein’s stability and structure.
Keywords: B-cell epitopes, Bacillus subtilis subsp. natto, Bioinformatics, Immungenicity, In-silico mutagenesis, Nattokinase
International Conference of Natural Resources and Life Sciences 2016 37
University of Surabaya (UBAYA)
OP-A-6 ALPHA MANGOSTIN EFFECT ON MDA LEVEL AND THE PANCREATIC MORPHOLOGY Rattus novergicus INDUCED ALLOXAN
Devyana Dyah Wulandari1, Zumrotul Mufidah1, Taslim Ersam2, Devyani Diah Wulansari3
1University of Nahdlatul Ulama Surabaya, 2Institut Teknologi Sepuluh Nopember, 3University of Surabaya *Corresponding author: [email protected]
Abstract Alpha mangostin compound is a single compound isolated from rind extract Garcinia mangostana Linn, known to have an antidiabetic bioactivity. The aimed of this research was to determine the effect of α-mangostin compound on MDA and and rats pancreatic tissue induced with type 2 diabetes mellitus. Methode: 24 Wistar male rats were divided into 4 groups, each group consisting of 6 rats. The first group was set as positive control, rats injected with single dose of 150 mg / kg alloxan. The second group was the negative control, mean that no treatment. The third group injected with a single dose of 150 mg/kg alloxan and a-mangostin compund 10 mg/kg, while the last group injected with a single dose of 150 mg/kg alloxan and 10 mg/kg standard drug glibenclamide. All groups were monitored for 3 weeks and blood sugar levels were measured using Glucosemeter. Blood samples were taken to measurement of MDA and pancreas organ was removed for histological study. MDA level in positive control group had a MDA value higher than the other groups (8.1771 ± 1.5614). MDA level in α-mangostin compound treatment group (6.9724 ± 1.1803), the negative control group (6.4906 ± 0.6464) and glibenclamide group (6,6111 ± 0.7106). Islets of langerhans on pancreatic normal mice (control (-)) looked filling full with the endocrine cells spread throughout the pancreas. While at the control group (+) there was a room-empty space (fewer the number of beta cells) in islets of Langerhans. On the treatment groups those were giving a-mangostin and drug compounds standard (glibenclamide) showed improvement Langerhans β cells compared with a treatment group of diabetic mice (control+). The administration of α-mangostin compound 10 mg/kg BW able to decrease MDA level 1,2031 nmol/dl. While based on histological observations pancreas, administration of alpha mangostin and Drug Compounds standard (glibenclamide) showed improvement compared Langerhans β cells with a treatment group of diabetic rats.
38 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
Keywords: Alpha mangostin, Malondialdehyde (MDA), Diabetes Mellitus type 2, pancreatic histology
International Conference of Natural Resources and Life Sciences 2016 39
University of Surabaya (UBAYA)
OP-A-7 In Vivo Efficiency of Collagen Extract from Sangkuriang Catfish (Clarias gariepinus var. Sangkuriang) Skin on MDA Levels and Macrophages Total in Burn Wound Healing
Andini Ary1, Handajani Retno2, Soetjipto2
1Faculty of Health, University of Nahdlatul Ulama Surabaya, Indonesia 2Departement of Biochemistry, Faculty of Medicine, University of Airlangga, Surabaya, Indonesia *Corresponding author: [email protected]
Abstract Topical collagen on skin wound gift moist state that could stimulate re- epithelization, proliferation and epithel cell migration to conduct growth factor activity on wound area. One of source collagen is Sangkuriang catfish skin that had low immunoreactivity in human body. The aim of this study was to determine the influence of topical treatment with Sangkuriang catfish skin extract on MDA serum and macrophage total on burn injury. Collagen of Sangkuriang catfish skin were extracted by HCl 2% for 48 hours and neutralized by NaOH 1 M. Sangkuriang catfish skin extract contained amino acid of collagen that could repaire and stimulate skin cell. This study was an experimental study that used twenty-four male Rattus novergicus strain Wistar were divided into K3, P3, K10, and P10 groups. K3 group was treated with aquadest topical as burn healing treatment for 3 days and K10 group was treated with aquadest topical for 10 days. P3 group was treated with topical collagen extract as burn healing treatment for 3 days, and P10 group was treated with collagen extract for 10 days. The results showed that treatment by collagen from Sangkuriang catfish skin could insignificantly decrease MDA serum on 3rdday (K3 and P3 groups) and on 10th day (K10 and P10 groups). Furthermore, it could decrease macrophage total insignificantly on 3rd day for K3 and P3 groups but significantly decrease on 10 days for K10 and P10.
Keywords: Sangkuriang catfish, collagen, malondialdehyde, macrophage, wound healing, burn injury
40 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-8 The Effect Of Folic Acid Supplementation In Parental Stress Mouse Toward NR3C1 Expression Alteration In The Offspring
Patricia*, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract Epigenetics is a pattern of inheritance of the traits of the parent to offspring without DNA sequence alteration. Paternal programming is a pattern of epigenetic inheritance issued by the father. Previous research on the "Overkalix study" shows that grandfathers with abundant food availability increase mortality and risk of diabetes and cardiovascular disease in grandchildren. Folic acid is an antidepressant drug that works by lowering homocysteine and increasing the methylation of DNA, proteins, lipids, and neurotransmitters. On the other hand, increase in methylation in the NR3C1 gene due to reduction of glucocorticoid receptor that leading to depression and other diseases. the effect of folic acid supplementation in paternal stress toward NR3C1 expression in the offspring isn’t known yet. In this study, the expression level of NR3C1 gene on mice (Mus musculus Balb/c) from paternal stress supplemented by folic acid was compared with the offspring of mice from paternal stress and control. The results of gene expression analysis showed significant decrease in gene expression in the offspring of mice from paternal stress compared to control (p <0.01). In addition, there was a trend of decreased gene expression in the offspring of mice from paternal stress supplemented by folic acid compared to paternal stress (p <0.1).
Keywords: paternal programming, epigenetic, stress, folic acid, NR3C1
International Conference of Natural Resources and Life Sciences 2016 41
University of Surabaya (UBAYA)
OP-A-9 In Silico Mutagenesis: Decreasing the Immunogenicity of Botulinum Toxin Type A
Stanley Evander Emeltan Tjoa*, Yoanes Maria Vianney, Sulistyo Emantoko Dwi Putra
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia Corresponding author: [email protected]
Abstract Botulinum toxin serotype A is one prominent therapeutic enzyme, both for clinical and cosmetic use. Since this protein is produced by bacteria, this protein exhibit allergenic effect when subjected to human therapy. Protein mutagenesis is one method to improve the characteristic of protein. However, in-silico study is needed to give suggestion which amino acid should be mutated, hence can save many cost and time. This paper gives suggestion which residue of the Botulinum toxin serotype A is B-cell epitopes both linearly and conformationally. By doing overlapping of those B-cell epitopes with the excluded conserve sequence, 7 residues were allowed to be mutated. There are two proposed mutein that showed a reduce in the antigenicity probability, which are ΔE147, E510F, T1062F, ΔE1080, N1089M, ΔQ1090; and ΔE147, E510F, T1062F, E1080W, N1089M, ΔQ1090. By doing the validation analysis with various tools and flexibility with molecular dynamics simulation of the 3D proposed muteins, both mutein was recommended. Both protein has a lower antigenicity but similar in structure and stability compared to the native protein.
Keywords: bioinformatics, botulinum toxin type A, B-cell epitopes prediction, immunogenicity, in-silico mutagenesis
42 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-10 Comparison of Food Intake Containing Vitamin D with BMI of Geriatric Patients in Sidoarjo, East Java
Rivan Virlando Suryadinata*, Sajuni Wijaya, Risma Ikawaty
Medical Faculty of Surabaya University *Corresponding author: [email protected]
Abstract Previous studies showed relationship of vitamin D deficiency with obesity. This study was based on observation of food intake containing vitamin D with BMI geriatric patients in Sidoarjo, East Java. We used case control design with semi- quantitative questionnaire. Eightythree obese geriatric patients and 83 non- obese geriatric patients (4.82% thin, 84.34% normal, and 10.84% at risk) were observed. Sampling technique was random sampling (non-probability sampling) with purposive sampling method and tested by using Chi square. From the obese group we found 76 patients had vitamin D deficiency and 7 patients had enough vitamin D supplementation, while in the non-obese group, 80 patients had deficiency of vitamin D intake, and 3 patients had enough vitamin D (p = 0,192 (p> 0,05)). Thus we concluded, there was no significant difference of food intake containing vitamin D on both group, obese and non- obese geriatric patients.
Keywords: Vitamin D, geriatric, obesity
International Conference of Natural Resources and Life Sciences 2016 43
University of Surabaya (UBAYA)
OP-A-11 Influenceof Folic Acid Diet on Maternal Stress to Offspring Stress Level and NR3C1Expression
Natanya*, Sulistyo Emantoko Dwi Putra, Ida Bagus Made Artadana
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract Stress has become the main topic on the subject of neurology and affective disorder. In terms of epigenetics, parental stress may cause neuropsychiatric difficulties to offspring. During stress, excess cortisol level happens duet a low expression of glucocorticoid gene (NR3C1). This is inherited to offsprings by supressing NR3C1 gene. To avoid stress exposure, folic acid intervention became one of the option as antidepressant. In this research, stressed mice model and the effect of folate diet is towards stress is made. Stress behaviour is test using forced swim test (FST) method. Female parent with space stress and darkness experienced stress, which can be seen from high immobile duration compare to mice without stress (p<0.05). While folate diet does not show any influence on the inheritance of levels of stress, seen by the difference in immobile duration of stressed female parent and stressed with intervention of folic acid diet (p=0,367). The result of this stressed model is then bred with normal male parent and its behaviour and level of NR3C1 expression is observed. On offspring, it is found that there is no significant different between the offspring of maternal stress and normal (p=0,174). Furthermore, there is a significant decrease (p<0,05) on the expression of NR3C1 on offspring of maternal stressed pair. This result shows that the potential to experience stress of offspring with a stressed female parent is higher than offspring of normal female parent.
Keywords: Glucocortioid receptor, epigenetic, folic acid, stress level, FST
44 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-12 Facile Green Synthesis of Silver Nanoparticles from Ethanol Extract of Plantago major L. and its antibacterial activity
Sukweenadhi Johan1, Setiawan Kezia Irianti2ϕ, Kartini2, Tjandrawinata Raymond Rubianto3, Avanti Christina2*
1Department of Plant Biotechnology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60293, East Java, Indonesia 2Department of Pharmaceutics, Faculty of Pharmacy, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60293, East Java, Indonesia. 3Dexa Laboratory of Biomolecular Science, Titan Center, 3rd Floor, Boulevard Bintaro, Block B7/B1, No. 5, Bintaro Jaya Sector 7, Tangerang 15224, Indonesia *Corresponding author: [email protected]
Abstract Nanomaterials from biological sources have received large attention due to broad range of utilization and less harmful for humans, animals and environmental-friendly. Our aim is to provide the nanoparticles to control infections in diabetic foot ulcer. In this present study, silver nanoparticles (PmAgNPs) were synthesized through green synthesis approach, using Plantago major L leaves extract. UV–visible absorption spectroscopy, Scanning Electron Microscopy, X-Ray Diffractometer and Fourier Transform Infra-Red spectroscopy analyses confirmed synthesis of silver nanoparticles. The stable non-aggregating nanoparticles were spherical in shape with an average size of 287.9 nm and has maximum absorbance at a wavelength of 436.6 nm. Characterization of the antimicrobial activity of synthesized nanoparticles by disk diffusion method showed strong growth inhibitory activity against all tested bacteria commonly infected to ulcer patient, ie. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. As evidence, the inhibition zones of PmAgNPs ranged from 57 to 78 mm. Less time, cost efficient and eco-friendly synthesis of biologically active silver nanoparticles from Plantago major L leaves extract not only cut down laborious downstream process, but also provides a reliable source of safe nanomaterials with potential application in diabetic foot ulcer treatment.
Keywords: Diabetic Foot Ulcer, Green synthesis, Silver Nanoparticles, Plantago major L., antibacterial activity
International Conference of Natural Resources and Life Sciences 2016 45
University of Surabaya (UBAYA)
OP-A-13 In Vitro Evaluation of Chitosan-DNA Plasmid Complex Encoding Jembrana Disease Virus Tat Protein as a Vaccine Candidate
Asmarani Kusumawati1,2*, Januar Ishak2, Lalu Unsun-Nidhal2 and Ronny Martien3
1Department of Reproduction, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Jalan Sekip unit II, Yogyakarta, 55281, Indonesia, email: asmaranikusumawati [email protected]; 2Research Center for Biotechnology, Universitas Gadjah Mada, Jalan Teknika Utara, Yogyakarta, 55281, Indonesia; 3Departement of Pharmaceutics, Universitas Gadjah Mada, Jalan Sekip Utara, Yogyakarta, 55281, Indonesia *Corresponding author: [email protected]
Abstract The development of Jembrana disease vaccine is importance to prevent the loss of Bali cattle industry in Indonesia. Delivery system is a vital factor for the success of DNA vaccine in inducing immunity. This study aims to prepare a Jembrana DNA vaccine encoding the tat gene in pcDNA3.1 and to test the success of its delivery in cells using DNA-chitosan complex. Cloning of the pcDNA3.1-tat has been successfully performed on E. coli DH5α and confirmed by PCR, restriction analysis and sequencing. The propagated plasmids were prepared as DNA-chitosan complex and physiochemically characterized using Particle Size Analyzer. Complex with a 1:2 (wt/wt) ratio of DNA and chitosan have a mean diameter of 268.5 nm and zeta potential +25.1 mV. In this research, the addition of TPP as cross linker agent (chitosan:TPP = 1:2 wt/wt) results in the increase of mean diameter to 718.1 nm and in the decrease of zeta potential to 4.7 mV. These two complexes were transfected to the HeLa cell line as in vitro model of eukaryotic cells. The DNA vaccine was successfully delivered into cells, shown by the presence of specific RT-PCR product (149 bp) from total RNA of transfected HeLa. The real-time PCR analysis showed that the delivery with chitosan complex resulted in higher tat mRNA expression when compared with delivery using chitosan-TPP complex.
Keywords: DNA-chitosan complex, Jembrana disease, tat gene, TPP
46 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-A-14 THE RELATIONSHIP BETWEEN ASTHMA CONTROL - METHYLXANTHINE USED AND CYP1A2*1F GENE POLYMORPHISMS
Mariana Wahjudi1*, Doddy de Queljoe2, Amelia Lorensia2, Muhammad Erdiansyah1, Yovita Selma Desiyana2, Eki Nuri Fauziyah2
1Laboratorium Purifikasi dan Biologi Molekuler, Fakultas Teknobiologi, Universitas Surabaya, Jl Raya Kalirungkut, Surabaya 2Laboratorium Farmasi Klinis – Komunitas, Fakultas Farmasi, Universitas Surabaya, , Jl Raya Kalirungkut, Surabaya *Corresponding author: [email protected]
Abctract The most frequently used methylxantine groups are theophylline and aminophylline. Both medicines are rarely used in others countries because of its narrow therapeutic effect with ADR (adverse drug reactions) risk. However in Indonesia, both medicines rarely caused any serious problems and even rarely cause ADR. This individual reaction is likely influenced by the CYP1A2*1F gene polymorphisms that contributes to theophylline metabolism in the liver. Therefore, this study was aimed to investigate the correlation between asthma control and methylxanthine used with CYP1A2*1F gene polymorphism. This research is a cross-sectional study with purposive sampling in the asthma outpatient setting. Data of asthma control and frequency of methylxanthine medication was taken by interviewing, while the CYP1A2*1F gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique. The data were analyzed using Chi-Square test. The result from the 32 adult-asthmatic-outpatients revealed that there was no relationship between asthma control with the CYP1A2*1F gene polymorphisms (sig. = 0.433). However, there was a positive correlation between ADR occurrence in theophylline/aminophylline medication with the CYP1A2*1F gene polymorphisms (sig. = 0.038). It is therefore necessary for pharmacists to monitor the use of theophylline / aminophylline regarding to the genetic and environmental influences.
Keywords: Asthma control, methylxantine, CYP1A2*1F gene polymorphisms
International Conference of Natural Resources and Life Sciences 2016 47
University of Surabaya (UBAYA)
AGRICULTURE
48 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-B-1 Plant Regeneration through Somatic Embryogenesis from Callus of Vanda tricolor Lindl. var. pallida
Popy Hartatie Hardjo*, Wina Dian Savitri and Ivon Grace Paulin
Faculty of Biotechnology, University of Surabaya Jalan Raya Kalirungkut, Surabaya 60292, East Java, Indonesia *Corresponding author: [email protected]
Abstract Somatic embryogenesis is a process where cells undergo sequence of development which similar to zygotic embryo’s development. In this study, we developed a protocol to induce indirect somatic embryogenesis from the basal leaf segments of Vanda tricolor Lindl. var. pallida. Embriogenic calli obtained from previous experiment were regenerated to form somatic embryo on half- strength MS medium supplemented with (0.05-0.2 mg.L-1) BAP or in combination with 0.01 mg.L-1 NAA. Embriogenic calli cultured on 0.05 mg.L-1 BAP incorporated with 0.01 mg.L-1 NAA were able to establish 90% somatic embryos (SEs)’ structure at 30 days of culture period. The pattern of SEs’ development started from embryogenic callus to form pro-embryo, followed by globular phase at 10 days of culture. Globular embryo elongated to form suspensor at 20 days of incubation period, and completed embryo. The fastest SE’s germination occurred on the half-strength MS medium with the addition of 0.02 mg.L-1 BAP.
Keywords: somatic embryogenesis, Vanda tricolor Lindl. var. pallida
International Conference of Natural Resources and Life Sciences 2016 49
University of Surabaya (UBAYA)
OP-B-2 Influence of Types and Concentration of Natural Pesticides to Mortality of Red Mite (Panony chuscitri McGregor)
Dyah Roeswitawati*1, Iva Kristova2 Muhidin1, Otto Endarto 3
1 Lecturer at Agotechnology Departement of Muhammadiyah University of Malang 2 student at Agotechnology Departement of Muhammadiyah University of Malang 3 Field Supervisor Indonesian Citrus and Tropical Fruits Research Institute Batu, East Java *Corresponding author: [email protected]
Abstract Red mites (Panonychuscitri McGregor) is one of the plant pest attacking leaves of citrus. That pest attack leaves on citrus plants leads to symptoms: patches of sterile or brown on fruit and yellow or brown spots on the leaves of oranges. Farmers often control this pest with drugs or chemical pesticides such as insecticides. Increased pest resistance against pesticides (resistance), making it less precise and can lead to poisoning for humans and ecosystems, the environment becomes unstable / unbalanced. Persistent pesticides leave residues that are difficult to clean. Usually farmers control the pest using chemical pesitisida and cause environmental damage due to toxins contained in the pesticide material. Chemical pesticides also result in increased pest resistance against pesticides. Persistent pesticides also leave residues that are difficult to clean in plants so that natural pesticides are needed as an alternative to chemical pesticides. The purpose of this research is to know the effect of vegetable pesticide concentration of soursop (Annona muricata) leaf, papaya (Carica papaya) leaf, Chrysanthemum leaves to red pest mortality and persistence of vegetable pesticide. The results showed that pesticide concentration 5% resulted in 100% mortality of imago phase and instar phase 1 at 48 hours observation. The highest mortality rate is the treatment of papaya leaves pesticide 5% concentration in the imago phase that is a number of 29.67
Keywords: Annonamuricata , Carica papaya, Chrysanthemum, mortality, persistence, red mites
50 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-B-3 Effect of salinity on biomass allocation and whole plant mass of rice (Oryza sativa L) related to growth at panicle initiation and heading stages
Niki Habibi*1, Nafisah2, Indah Trisnawati D.T.1, Mukhammad Muryono1
1Departement of Biology, Institut Teknologi Sepuluh Nopember, Kampus ITS- Keputih Sukolilo, Surabaya 60111, Indonesia. Telp. +62 31-5963857, Fax +62 31-5963857, E-mail [email protected]. 2Indonesian Agency for Agricultural Research and Development Ministry of Agricultural, Ragunan street 29 Pasar Minggu, Jakarta Selatan 12540, Indonesia.Phone +62 21-7806202 Corresponding author: [email protected]
Abstract Rice (Oryza sativa L.) is the most important staple food and provided most of the daily calories for the world population. In South East Asia, 40% of rice production is from a deltaic region that is vulnerable to salt stress. Meanwhile, the suboptimal saline area in Indonesia has wide open agricultural extensification opportunities, covering an area of 0.4 million hectares. Rice is considered to be moderately sensitive to salinity. The sensitivity of rice to salinity stress varies with the growth stage. A physiological response was developed for characterizing varieties adapted to salt-affected areas. Four varieties we evaluated in the field under the saline condition, with two soil salinity level, low saline on 4 dSm-1 and high saline on 8 dSm-1. Plant mass, green leaf number, shoot mass, and roots mass were measured at the vegetative stage. For four varieties tested at both saline conditions, the [saline] x [variety] interaction was significant for plant mass, shoot mass, root mass, but there was no significant for green leaf number. Interaction with a stage was also significant for all parameters observed. Plant mass at both stages was closely correlated with root mass and leaf number over salinity level and varieties. Root mass and leaf number might be a target in future rice breeding under salt stress for increasing dry matter production.
Keywords: Rice (Oryza sativa L.), Salinity, Biomass allocation, Whole plant mass, Growth.
International Conference of Natural Resources and Life Sciences 2016 51
University of Surabaya (UBAYA)
OP-B-4 Study of Ccs and pAMT Gene of Genotype 1 Cayenne Pepper (Capsicum frutescens L.) Original Type and Mutant
Nur Rahmattullah*, Wahyuningyan Arini, Ginting Dwi Setyawan, Estri Laras Arumingtyas
Department of Biology, Faculty of Mathematics and Sciences, University of Brawijaya, 1st Veteran street, Malang, Indonesia *Corresponding author: [email protected]
Abstract Cayenner pepper (Capsicum frutescens L.) is a potential commodity cultivated in Indonesia and effort to establish superior varieties have been done, one of them by using ethyl methanesulphonate (EMS) as mutagent. This study aimed to to (1) analyze the change of the sequences of Ccs and pAMT genes in the G1 cayenne pepper original type (OT) and mutant; (2) investigate carotenoid and capsaicin content that might correspond to gene mutation. The Ccs and pAMT from G1 OT and mutant were analyzed through sequencing and aligned with Capsicum annuum from GeneBank. Carotenoid and capsaicin content were measured from three different fruit age, i.e. 30, 45 and 60 days after pollination (DAP). The change caused sequence of pAMT and Ccs gene of G1 cayenne pepper different from the G1 OT, but same as Capsicum annuum cv. Himo. The alternation of C (cytosine) to T (thymine) nucleotide in 809 bp of Ccs that cause isoleucine-arginine alternation might has the impact physiologically. Overall, compared with OT plant, carotenoid and capsaicin in mutant showed higher value and had shorter optimum fruit age based on cut off point analysis. Hence, mutation through EMS might establish superior varieties in future.
Keywords: cayenne pepper, carotenoid and capsaicin, days after pollination, gene profile, nucleotide alternation, reverse mutation
52 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-B-5 Menthol from stem and leaf invitro Mentha piperita
Bintang Kerta Wijaya*, Popy Hartatie Hardjo, Sulistyo Emantoko Dwi Putra
Faculty of Biotechnology, University of Surabaya, Kalirungkut Road, 60292, Surabaya, East Java, Indonesia *Corresponding author: [email protected]
Abstract The need for menthol in Indonesia is increasing annually, but it is not followed by increasing in the availability in the country so the number of imports is increasing each year. Therefore, in this research we conducted a study using plant tissue culture techniques to produce secondary metabolite especially menthol using shoot multiplication. Single node of Mentha piperita were inoculated on three kinds of growth medium, which are MS + 0,1 ppm NAA + 0,1 ppm BA, MS + 0,5 ppm BA, and MS + 2 ppm BA. Based on fresh weight and morphological observation, MS + 0,1 ppm NAA + 0,1 ppm BA was the best growth medium. Based on the previous experiment, the study was aiming to continue observing the concentration of menthol found in Mentha piperita shoot. Stems and leaves of Mentha piperita were dried in room temperature, the dried stems and leaves were extracted using steam distillation method with ratio 0,3 grams of dried stems and leaves in 25 mL aquadest. The crude extract was analysed by gas chromatography (HP 6890) using INNOWAX 19095N-123 column. The results showed that two weeks was the best amount of time to obtain highest concentration of menthol specifically 1,218.5 ± 47.1 ppm (yield = 9.748%).
Keywords: Mentha piperita, menthol, shoot multiplication, steam distillation, gas chromatography
International Conference of Natural Resources and Life Sciences 2016 53
University of Surabaya (UBAYA)
OP-B-6 Analysis of Abscisic Acid, Salicylic Acid and GABA in Several Oil Palm Progenies in Drought Stress
Susy SAADAH*, Endri PURWANTI, Randi Abdur ROHMAN, Nurita TORUAN-MATHIUS1, Tony LIWANG1
PT SMART Tbk *Corresponding author: [email protected]
Abstract Metabolites are key players in signaling environmental stress conditions. This study aims to compare the concentration level of abscisic acid, salicylic acid, and gamma-aminobutyric acid (GABA) among tolerant and susceptible oil palm progenies of drought stress. The experiment was arranged in factorial randomized complete block design consisted of three factors: drought stress (watering and non-watering), progenies (S1, G2, G3, G4, G5 and G6), and duration of treatments (0, 7 and 14 days). The samples were taken from leaves and roots tissues. The result showed that abscisic acid, salicylic acid, and GABA were significantly elevated in leave samples of tolerant progenies at 7- days of non-watering treatment. However, abscisic acid, salicylic acid, and GABA were not significantly produced in roots samples of tolerant progenies at 14-days of non-watering treatment.
Keywords: abscisic acid, drought, GABA, oil palm, salicylic acid
54 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-B-7 Uptake of Macro Nutrients and Growth of Mahang plants (Macaranga gigantea)
Dwi Susanto*1, Rudianto Amirta2
1Department of Biology, Faculty of Mathematics and Natural Sciences, Mulawarman University, Jl. Barong Tongkok No. 4, Gunung Kelua, Samarinda, Indonesia 2Faculty of Forestry, Mulawarman University, Indonesia, Jl. Ki Hajar Dewantara Kampus Gunung Kelua, Samarinda, Indonesia *Corresponding author: [email protected]
Abstract Macaranga gigantea plant is one species of the secondary tropical rain forest in East Kalimantan. This plant in addition to potentially as a medicinal plant, also has potential as industrial wood raw materials. The study was conducted for 6 months at the Botanical Garden of Mulawarman University Samarinda. The aims of this study is to determine the uptake of macro nutrients and the growth of M. gigantea plant with application of NPK fertilizer. The research was done by randomized block design, the fertilizer treatment consisted of four levels, P0 = 40 g, P1 = 80 g, P2 = 120 g and P4 = 160 g per plant, each with 20 replications. The results showed that fertilization increased nutrient uptake and M. gigantea plant growth. At the beginning of growth, this plant accumulates a lot of magnesium, potassium, calcium, followed by nitrogen and at least is a phosphorus nutrient. Fertilization application also increases plant height, leaf number, stem diameter and crown diameter.
Keywords: Macaranga gigantea, growth, nutrient uptake
International Conference of Natural Resources and Life Sciences 2016 55
University of Surabaya (UBAYA)
OP-B-8 The Evaluation of Breeding Value and Most Probable Producing Ability for the Basis Selection of Ettawa Croosbred Goat at Malang Region, East Java, Indonesia
Aris Winaya*, Suyatno1 and Nurul Fauzi
Faculty of Agriculture and Animal Science, University of Muhammadiyah Malang *Corresponding author: [email protected]
Abstract Ettawa crossbred goat is Indonesia local breed goat that genetically has also advantages regarding milk production. But, these goats have not maximally developed for milk production. The continual selection of the breed was important to improve the productivity of Ettawa crossbred. The selection program can be an effective effort to increase the milk production of Ettawa crossbred goat. The Breeding Value (BV) and Most Probable Producing Ability (MPPA) could be considered on selection program on goat breed. The purpose of this study was to determine the differences in milk production of Ettawa crossbred in Malang region and to know the BV and MPPA values of Ettawa crossbred goats. This research was conducted by direct survey method on goat farmers in the sample areas. The second lactation of the milk production was stated as the data variable. The analysis of variance from nested design showed that any significance different (P<0.05) in milk production between regency/city population and very significant (P<0.01) in milk production between districts population of Malang region. The Least Significant Difference (LSD) test on sub-district of Malang Regency showed that both Ampelgading with Singosari sub-district and Lawang with Singosari sub- district were high significance different (P<0.01), but Ampelgading with Lawang sub-district was not significantly different (P>0.05). At Batu Regency showed that Bumiaji with Pesanggrahan sub-district was significantly different (P<0.05) while Bumiaji with Junrejo and Junrejo with Pesanggrahan were not significantly different (P>0.05). The highest BV was 0,389 litre from Bumiaji sub-district and the highest MPPA was 0.595 liter from Bumiaji sub-district also. So, from the BV and MPPA values Ettawa crossbred goat from Bumiaji sub-district of Batu Regency could be chosen as the basis population on the breeding program of dairy milk in Malang region.
Keywords: Breeding Values, dairy milk, Ettawa goat, Malang region, MPPA
56 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-B-9 IN VITRO MULTIPLICATION OF FIG (Ficus carica L.) FOR COMMERCIALLY INDUSTRY
Ivon Grace Paulin*, Johan Sukweenadhi, and Popy Hartatie Hardjo
Faculty of Biotechnology, University of Surabaya Jalan Raya Kalirungkut, Surabaya 60292, East Java, Indonesia * Corresponding author: [email protected]
Abstract The most common fruit has planted in Turkey and Mediterranean region is fig fruit (Ficus carica L.). It has been an important food crop for thousands of years and in the world market, there is an increasing demand for fresh figs and a stable demand for dried figs. This fruit also contain a lot of benefit nutritional and phytochemistry function. So, probably we have to develop this edible fruits in Indonesia, which commonly have the same tropical like in Mediterranean region. Furthermore, the technique such as in vitro culture has advantage of large-scale production and providing plantlets as seeds. In this review, we discuss several results of propagation fig fruit from various condition of explants and combination growth hormone in media in tissue culture Ficus carica L. Multiplication from shoots used combination MS + 1 mg /L IBA + 1 mg /LGA3 + 5 mg /L BA. Callus induction development in culture medium supplemented with 2 mg/l TDZ and 4 mg/l IBA. In the other hand, activated charcoal, ascorbic acid 0.1% and phloroglucinol were added to prevent negative effect of polyphenols from explants. Since several research in tissue culture of Ficus carica L. and they have been successful for clonal multiplication by shoot or organogenesis by callus induction. It still needs the development of Fig protocol for each cultivar to support other research and can be used more easily by the community in developing commercial needs.
Keyword: Ficus carica L., callus induction, in vitro multiplication
International Conference of Natural Resources and Life Sciences 2016 57
University of Surabaya (UBAYA)
OP-B-10 Mild Alkaline Pretreatment on Sugarcane Bagasse: Effects of Time and Lime Ratio to Dry Bagasse
Lieke Riadi1, Maria G.M. Purwanto2, Jesslyn Pratiwi1, Yoelsando Hansen1
1Chemical Engineering Department, University of Surabaya, Surabaya, 60293, Indonesia 2Faculty of Biotechnology, University of Surabaya, Surabaya, 60293, Indonesia * Corresponding author: [email protected]
Abstract Sugarcane waste is organic waste produced at about 8,000,000 tons/year. This lignocellulosic waste can be used for raw material of bioethanol manufacture through pretreatment, hydrolysis, and fermentation process. This study aims to determine the yield (g/g) of glucose produced from pretreatment by using lime (Ca(OH)2) and enzymatic hydrolysis. The variables used in this research were pretreatment time and lime ratio to bagasse in the pretreatment step. The results showed that pretreatment time gave a stronger effect on the glucose yield compared to lime:bagasse ratio did. Longer pretreatment time up to 180 min increased the glucose content of the pretreated bagasse, but after 180 min of pretreatment, glucose content was decreased. While higher ratio of lime to bagasse in pretreatment process decreased the glucose content. The best glucose content (0.071 g/g) was obtained from pretreatment process with a ratio of 0.5 g Ca(OH)2/g bagasse for 180 min and ethanol content of 5.704 ± 0.15 g/L.
Keyword: Bagasse, Alkaline pretreatment, Bioethanol, Enzymatic hydrolysis
58 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
ENVIRONMENT & ENERGY
International Conference of Natural Resources and Life Sciences 2016 59
University of Surabaya (UBAYA)
OP-C-1 Utilization Cytochrome B Gene for Phylogeny Analysis of Cuora amboinensis to Prevent Extinction: Preliminary Study
Dwi Martha Nur Aditya*1 and Mohamad Amin2
1Medical Faculty of Ubaya University 2State University of Malang *Corresponding author: [email protected]
Abstract Cuora amboinensis is widespread distributed and yet these species is classified as vulnerable since they are at high risk of extinct. It is also known that C. amboinensis does not migrate seasonally or to any geographically significant extent which causes chances of inbreeding even greater. Phylogeny analysis are required to reveal the kinship relationship of Genus Cuora, so it can be used as a preliminary study of conservation by determining outbreeding. We compared Cytochrome b in C. amboinensis samples from Kendari, Toli-toli, and Ambon regions to that C. picturata (Vietnam), C. galbinifrons (China), C. flavomarginata (China), C. serrata (China), and C. mouhotti (China). Technology we used including DNA preparation, Primer Design, Polymerase Chain Reaction (PCR), horizontal electrophoresis, and sequencing. We than compared the Cyt b sequences to the reference species obtained from the NCBI Genbank. Further, phylogeny analysis is performed by using MEGA 6.0 with Maximum Likelihood (ML) methods. The result showed close kin relationship between of C. amboinensis of Ambon, Kendari, and Toli-toli regions with most of genetic distance below 2.1, while Ambon-I and Kendari-11 have measured the longest 33.63 and 38.18, respectively.
Keywords: Cuora, genetic distance, phylogeny analysis, Cyt b
60 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-C-2 Profile of Antibiotic CompoundsfromTrichoderma harzianumin Controlling Aspergillus flavuson Bali Lontar
I B G Darmayasa*, Retno Kawuri
Microbiology Laboratory Faculty of Mathematics and Natural Sciences, Udayana University Corresponding author: [email protected]
Abstract Lontar is a descent of ancestors that should be preserved. The lontar script contains the script of Balinese script that is classical or ancient which is often referred to as manuscript and is a cultural tradition of writing in Bali that tend to be sacred and religious (Geriani, 2010, Sedana et al., 2013). Lontar stored in the community did not escape the damage and one cause of the damage is Aspergillus flavus. Trichoderma is a group of fungi that are believed to have the ability to inhibit the growth of other microorganisms. The objective of the study was to find out the profile of antibiotic compounds produced by Trichoderma sp. which will be utilized in controlling A. flavus on the palm of Bali. Isolation of A.flavus and Trichoderma sp. done by using plating method with dilution series.Dual cultural method is used to determine the capability of Trichoderma sp. against A.flavus. Extraction filtrate of Trichoderma sp. using diffusion well method. Profile of antibiotic compounds Trichoderma sp. known by using colomn technique and GCMS (Gas Chromatography Mass Spectrometry). The result of fungal isolation on lontar was identified as A. flavus and on peanut plant rhizosper was identified as Trichoderma hazinarum which had the ability to inhibit A. flavus by 69,5 ± 1.56%. The extract of the filtrate obtained is able to form a zone of 15.32 ± 0.277 mm.The identified 15 compounds present in the filtrate extract were suspected to be antibiotics ie Benzene, 1,3,5-trimethyl- (CAS) 1,3,5-trimethyl; 2-Azabicylol (3,2,1) octan-3-one; 5,6-Dihydro-4- methyl-2H-Pyran-2-C; 2-Butenoic acid, 2-methyl- (CAS) 2-methyl-2; 2H- Pyran-2-one, tetrahydro-4-hydroxy-4-methyl; 2-Bromo dodecane; Lyxitol, 1-O- nonyl; Pentadecane, 3-methyl- (CAS) 3-methylpentax; Trichloroacetic acid, tridecyl ester; Heptadecane, 3-methyl- (CAS) 3-methylhepta; n-Hexadecanoic acid; Nonadecane, 3-methyl- (CAS) 3-Methylnonad; Octadecanoic acid; 3- Methylheneiccosane and Heptadecane, 3-methyl.
Keywords: Antibiotic, Trichoderma harzianum, Aspergillus flavus, extract, filtrate
International Conference of Natural Resources and Life Sciences 2016 61
University of Surabaya (UBAYA)
OP-C-3 Plasmid Curing Analysis of Chromate Reducing Bacteria Isolated from Serpentine Soil
Badruzsaufari*, Aisyah, Hasrul Satria Nur, and Dindin Hidayatul Mursyidin
Department of Biology of Faculty of Sciences and Mathematics of the Lambung Mangkurat University. *Corresponding author :[email protected]
Abstract Chromate resistant bacteria that isolated from Serpentine soil of Mandiangin and Awang Bangkal villages of Kalimantan Selatan were capable to reduce chromium hexavalent (Cr6+) into non-toxic chromium species (Cr3+). It is suggested that the bacteria have a potential to be developed as biological agents in bioremediation of chromium contamination. The chromium resistant mechanisms of the bacteria which involve the responsibility of their plasmids should be revealed. The research included identification of the bacteria isolates using 16 S rRNA gene sequences, examination of the isolate chromium reduction capability, and elimination of the bacterial plasmids by culturing the isolates on 0.1 % SDS containing LB media. Two bacterial isolates AB13 and AB56ACr10 were found to have 99% of their 16 S rRNA gene sequences similar to Bacillus cereus while the isolate M2Cr10 showed 99% similarity to Acinetobacter radioresistens. All isolates were able to reduce 13 mg.L- Cr (VI) up to 90 % for 24 H incubation at 30oC. The isolate AB56ACr10 could resist the present of 150 mg.L- of Cr (VI) in the growth media for 24 H, but the other isolates could only tolerate 100 mg.L- of chromium hexavalent. The three isolates had 2 plasmids which 55 kb and 10 kb in size of which the latter was removed from the bacterial cell when growing on 0.1 % SDS containing LB media. The plasmid cured isolates could not be able to grow on 50 mg.L- of Cr (VI) contaning media and reduced their resistance to ampicilin, streptomycin, and chlorampheniol antibiotics.
Keywords: bacteria, bioremediation, chromium, plasmid curing, serpentine soil
62 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-C-4 Effect of Cycle Time on Biokinetics Parameters in Tapioca Wastewater Treatment Using Sequencing Batch Reactor
Happy Mulyani*, Gregorius Prima Indra Budianto
Setia Budi University, Jl Letjend Sutoyo, Mojosongo, Surakarta, Indonesia, *Corresponding author: [email protected]
Abstract In this work, the effect of cycle time on kinetics parameters of sequencing batch reactor (SBR) system of tapioca wastewater treatment was evaluated. First, the SBR was operated under three different variations of filling time (0.5, 1, 1,5 hour) with an aeration time of 9 h. After reached a steady state, liquid samples of SBR system at one-half hour intervals were collected for analysis of pH, Chemical Oxygen Demand (COD) and Mixed Liquor Suspended Solids (MLSS) for optimum filling time determination. The optimum filling time results in SBR operation with the best values of biokinetics parameters considering pH and substrate inhibition. Then, SBR was operated once again with the cycle period: filling (optimum condition at first operation), aeration (9 h), and settling (9 h) to determine the effect of time of aeration and sedimentation on poly-β-hydroxybutyrate (PHB) yield. Research results shows that SBR operation with filling time 1 h was achieved best values of biokinetics parameters. The biggest PHB yield is achieved in aeration phase of SBR. Excessive aeration time and settling time addition contributes to PHB yield decrease.
Keywords: Biokinetics Parameters, Cycle Time, Poly-β-hydroxybutyrate, Sequencing Batch Reactor, Tapioca Wastewater
International Conference of Natural Resources and Life Sciences 2016 63
University of Surabaya (UBAYA)
OP-C-5 The utilization of tofu waste for the cultivation of Spirulina for biomass production
Yalun Arifin*1, Faisal Amri Tanjung2, and Ryanto Kurniadi3
1Department of Food Business Technology, Prasetiya Mulya University, BSD Raya Utama, Kabupaten Tangerang 15339, Indonesia. 2Center for Studies on Hasyim Asy'ari Thoughts, Universitas Hasyim Asy'ari, Jalan Irian Jaya No. 55, Tebuireng, Jombang, East Java, 61471, Indonesia 3PT. Nugen Bioscience Indonesia, Jalan Parangtritis Raya blok A5E/12, Ancol Barat, Jakarta 14430, Indonesia *Corresponding author: [email protected]
Abstract Spirulina (Arthrospira platensis) is one of the algae with market that is developing rapidly. The application includes for food supplement, animal feed, cosmetics and production of specialty chemicals obtained during the extraction of the algae biomass. The current market, however, is limited by the cost of production, particularly when synthetic chemicals are used as the growth medium. The use of industrial waste and agricultural by-product may reduce the production cost. Our research is using liquid waste obtained from tofu manufacturing process for production of Spirulina biomass. In this media, the cell performed mixotrophic metabolism that allowed higher cell productivity. The growth in 16- and 25-fold dilution of the liquid waste produced highest biomass productivity and cell concentration at 14 mg/l-day and 0.1 g/l, nearly three times higher than the values obtained from the cultivation in a control medium using synthetic chemicals. Higher concentration of liquid waste inhibited the cell growth. The biomass composition and the cellular phycocyanin level of the cell growing in the tofu waste is similar to the composition produced from growing in the control medium. This utilization of the liquid tofu waste reduced the cost for medium until 40 % lower. These results demonstrate the potential of using liquid waste from tofu manufacturing process as an alternative low cost and efficient growth medium for Spirulina biomass production.
Keywords: Spirulina, tofu waste, low cost medium, algae biomass
64 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-C-6 Thermostable Chitinase-Producing Bacterium from Ijen Hot Spring – Indonesia: Isolation, Identification and Characterization
Ruth Chrisnasari*, Dian Paulina, Liony Priscilla Sutanto, Tjandra Pantjajani, Maria Goretti Marianti Purwanto
Department of Biology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: ruth_c @staff.ubaya.ac.id
Abstract The high pharmaceutical industrial demand of thermostable chitinase with new desirable characteristics has lead to the exploration of chitinolytic bacteria from extreme environment. Therefore, in this study chitinase-producing bacteria were isolated from Ijen hot spring and screened. The highest chitinolytic activity bacterium was identified by 16S rRNA gene sequencing and its characteristics were confirmed by morphological and physiological analyses. The growth and chitinase production of the identified isolate were further characterized under variation of agitation and aeration. The molecular, morphological and physiological analyses confirmed that the isolated bacterium belonged to Bacillus licheniformis. This isolate produced huge amount of chitinase on Thermus colloidal chitin medium at 50 °C within 30 hours. The highest growth and chitinase production were recorded at 3 vvm aeration and 300 rpm agitation. Chitinase produced by this isolate was evidently proofed as thermostable through its high residual activity after several thermal stability tests.
Keywords: isolation, characterization, thermostable chitinase, Bacillus licheniformis, aeration, agitation
International Conference of Natural Resources and Life Sciences 2016 65
University of Surabaya (UBAYA)
FOOD & NUTRITION
66 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-D-1 Effect of Phosphoric Acid Pretreatment on Characterization of Gelatin from Broiler Chicken Bones
Dewi Yuliani*, Akyunul Jannah, Anik Maunati, Hanifah Hasna Fauziyah
1 Universitas Islam Negeri Maulana Malik Ibrahim Malang *Corresponding author: [email protected]
Abstract Chicken bone is one of the halal alternative sources of collagen fiber. This collagen, one kind of protein, can be hydrolyzed to form gelatin. The objective of this research was to extract and characterize chicken bone gelatin. Chicken bones were pretreated with phosphoric acid in varying concentrations and curing times. The concentration of phosphoric acid was 8, 9 and 10%, while curing time was 12 and 24 hours. Then, gelatin was extracted using hot water before the yield and physicochemical properties were examined. The result showed phosphoric acid 9% with 24 hours gave the highest yield, 15.4±0.94%. Water content, ash content, and pH of chicken bone gelatin were 3.96±1.46%, 24.0±6.36%, and 5.74±0.003%, respectively. Other physiochemical properties were 372.29±4.62% of gel strength and 54.21±1.24% of protein content. Based on Indonesia National Standard (SNI), water content and pH value fulfilled the requirement, but ash content was out of the requirement. Value of gel strength is higher than British Standard gave rigid and solid texture. Infrared spectra of chicken bone gelatin had the most with vibration peak at the wavenumber of 1639.74 cm-1 to the amide I, of 1545.94 cm-1 to the amide II, of 1129.42 cm-1 to the amide III, of 2.2927.98 cm-1 to the amide B, and 3456.26 cm-1 to the amide A.
Keywords: Characterization, chicken bone, gelatin extraction, FTIR, phosphoricacid, physicochemical properties.
International Conference of Natural Resources and Life Sciences 2016 67
University of Surabaya (UBAYA)
OP-D-2 Molecular Characterization of Potentially Probiotic Bacterium Isolate from Mackerel Fish (Rastrelliger sp.)
Yulia Sari Ismail*1, Febriani2, CutYulvizar3, Rasistia Ramadhani3
1 Biology Department, Mathematics and Science Faculty, Syiah Kuala University, Banda Aceh, Indonesia. 2 Chemistry Department, Mathematics and Science Faculty, Syiah Kuala University, Banda Aceh, Indonesia. 3 Biology Department, Mathematics and Science Faculty, Syiah Kuala University, Banda Aceh, Indonesia. *Corresponding author: [email protected]
Abstract The use of probiotics in aquaculture can improve the quality and quantity of the fishes. One of potencial probiotic bacterium isolate from gastrointestine of Mackerel fish (Rastrelliger sp.) that isolated in this research were LUI-04. This study was aimed to determine the type of bacterium isolate using nucleotide sequence homology compared to other microorganisms in GenBank. The 16S rRNA genes of LUI-04 bacterium isolate were analyzed. The results of electrophoresis showed that the DNA band had a size of 1500 bp for amplification using Bact-27F and Uni-1492R primers, and about 250-500 bp with the Com-IF and Com-2R primers. The sequence of nucleotides was determined by Sanger sequencing method. The 16S rRNA sequence of the isolate and other bacteria in GenBank were compared with the Basic Alignment Search Tool (BLAST) to know the homology of sequence. Then, all of the sequence were aligned by ClustalW, and the phylogenetic tree was reconstructed with Neighbor Joining method using bootstrap analysis of 1000 replicates. The results showed that LUI-04 had a homology of 99% and the percentage bootstrap value of 86% with one strain of Bacillus aryabhattai B8W22 and three strains of Bacillus megaterium (strain QM B1551, NBRC15308, and ATCC 14581). Therefore, the LUI-04 bacterium isolate belongs to Bacillus megaterium species.
Keywords: 16S rRNA gene, phylogenetic tree, potencial probiotic bacteria, Rastrelliger sp.
68 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-D-3 PROFILLING OF SHRIMP SHELL HYDROLYSIS PRODUCT BY CHITINASE FROM Bacillus licheniformisB2
Maria Goretti M. Purwanto*, Ruth Chrisnasari, Nalista Jayanthi
Department of Biology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: [email protected]
Abstract The application of chitin in the area of food and biomedicine is limited due to its insolubility in aqueous solvents. However, its hydrolysis product, N-acetyl chitooligosaccharides, is water- and acid- soluble. Bacillus licheniformis B2 isolated in previous study from Ijen Crater, Banyuwangi, East Java, appeared to be potential as chitin degrader bacteria. The aim of this study was to characterize the products of shrimp shell chitin degradation by chitinase from B. licheniformis B2. The hydrolysis products were analyzed using High Performance Liquid Chromatography (HPLC) with Zorbax Carbohydrate column and acetonitrile:water (70:30) as the isocratic mobile phase. Two major signals were detected at retention time of ±5.9 and ±7.8 minutes, assumed come from N-acetyl Glucosamine (C8H15NO6) and its dimer, N-acetyl chitooligosaccharides (C16H28N2O11), respectively. This study also showed that the hydrolysis product has antioxidant activity (57,.29-58.85%) with no antimicrobial activity. This data may contribute to the production of N-acetyl Glucosamine (C8H15NO6) and dimer N-acetyl chitooligosaccharides (C16H28N2O11) from shrimp shell especially for nutraceticals.
Keywords: Bacillus licheniformis B2, chitin, chitinase, N-acetyl chitooligosaccharide
International Conference of Natural Resources and Life Sciences 2016 69
University of Surabaya (UBAYA)
OP-D-4 Production, Purification and Characterization of Chitinase from Bacillus licheniformis D11
Adelia Angelin Anastasia, Ruth Chrisnasari*, Aime Clorinda Tapatfeto, Yonatan Imanuel Krisnajayadi, Tjandra Pantjajani, Maria Goretti Marianti Purwanto
Department of Biology, Faculty of Biotechnology, Surabaya University, Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: ruth_c @staff.ubaya.ac.id
Abstract Thermostable chitinase is an important enzyme in the utilization of shellfish wastes to solves environmental problem. In this experiment, the enzyme produced by Bacillus licheniformis D11 that was isolated from Cangar hot spring in East Java. The enzyme was purified and its kinetic characterized afterwards. Optimum production of chitinase was obtained when using 0,9 % of colloidal chitin and culture medium with pH 7 at 52 oC for 30 hours. The produced chitinase hydrolize chitin efficiently at 59 oC and pH 7. The enzyme residual activity was 64 %, 44 %, 48 %, 41 % and 31 % after incubated for 2 hours at 50 oC, 60 oC, 70 oC, 80 oC and 90 oC respectively, which proof thermostability of this enzyme. The crude extract then was purified by ultrafiltration followed by GFC using Sephadex G-100 resulting 29.86 % of yield and 193.81 fold of purity enhancement. Highest activity of GFC fraction was visualized by Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE) and showed three bands with molecular weight of 54 kDa, 58 kDa and 69 kDa. The same characteristic of GFC fraction was collected to be analyze its kinetic activity. The kinetic activity curve demonstrated an allosteric regulation of chitinase activity. Hence, Hill analysis of binding data performed, that reveal chitinase had a positive cooperativity (n value > 1) with chitin when Na+, K+ and Mg2+ ions added.
Keywords: enzyme kinetics, characterization, chitinase, purification, Bacillus licheniformis
70 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-D-5 PURIFICATION AND CHARACTERIZATION OF CHITINASE FROM Paenibacillus sp.
Maria Fidelia Susanto, Ruth Chrisnasari*, Maria Goretti Marianti Purwanto
Department of Biology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: ruth_c @staff.ubaya.ac.id
Abstract Chitin is the second most abundant polymer in the world. By using enzymatic process of chitinase, chitin can be degraded into its derivates which have many bioactive functions. In this project, Paenibacillus sp. isolated from Prataan hot spring in Tuban, Indonesia is used as a chitinase producing bacterium. Chitinase from this isolate is shown to be thermostable. To produce chitinase, 0.9% of colloidal chitin from shrimp shells in thermus broth was used as substrate for Paenibacillus sp. The fermentation process was run for 36 hours at 48 C. The agitation used was 200 rpm, with 3 vvm of aeration. The crude chitinase was harvested using centrifugation from fermentation broth. The supernatant then purified using ultra-filtration membrane followed by Ion Exchange Chromatography (IEC). The purified protein size was determined using Sodium Dodecyl Sulfate-Polyacrylamide Gel Electrophoresis (SDS- PAGE). The purified chitinase was also being characterized for enzyme kinetics activity using various concentrations of colloidal chitin and metal ion (Mg2+ and K+) addition. The result of this study showed that the purified chitinase was obtained between fraction number 15 and 20 during the IEC process and sized around 65 kDa. The purification process yielded 0.48% of chitinase and the chitinase was purer 1139.42 times compared to the crude extract. The enzyme demonstrated an allosteric regulation and the substrates showed positive coordination to enzyme’s binding sites, with the added metal ion worked as inhibitors.
Keywords: Paenibacillus sp., chitinase, purification, enzyme kinetics, allosteric enzyme, positive cooperativity
International Conference of Natural Resources and Life Sciences 2016 71
University of Surabaya (UBAYA)
OP-D-6 Rapid reversed-phase high performance liquid chromatography assay of tert-Butylhydroquinone content in food products
Steven Sutanto1*and Maria Goretti Marianti Purwanto1
1Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, East Java, Indonesia *Corresponding author: [email protected]
Abstract The use of antioxidant has been widely applied in food industry to maintain the product’s condition to the end consumer. Tert-Butylhydroquinone (TBHQ) is a synthetic aromatic organic compound which is often used as antioxidant in food, especially oil product and its derivatives. The use of TBHQ in food products has been limited by the government in most countries, including Indonesia. Therefore, the need for a rapid method to assay the concentration of TBHQ in products is quite high in food industry. In this article, we propose a rapid reversed-phase HPLC method to assay TBHQ content in food products. The isocratic system using 1% acetic acid and acetonitrile in 3:2 ratio with 1 mL.min- 1 flow rate produce good separation of TBHQ. Target peak was eluted in 5 minutes and the whole procedure only need maximum of 18 minutes to elute all the sample component. The LOD of the method was determined at 0.2 mg/L, while the lower limit of quantification was 0.5 mg/L. The accuracy of the method has been proved with acceptable recovery ranging from 95% to 105%.
Keywords: antioxidant, HPLC, reversed-phase, TBHQ
72 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-D-7 CHARACTERIZATION OF THERMOSTABLE CHITINASE FROM Bacillus licheniformis B2
Nalista Jayanthi, Maria Goretti M. Purwanto*, Ruth Chrisnasari, Tjandra Pantjajani, Alicia Wahjudi, Monica Sugiarto
Department of Biology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia *Corresponding author: [email protected]
Abstract Chitinases is an enzyme capable of degrading chitin into oligomers to produce chitin derivatives products which are more useful. Chitinase was obtained from Bacillus licheniformis B2 isolated from Ijen hot spring, East Java. The chitinase has the best chitinolytic activity at pH 7 when colloidal chitin was used as substrate. The enzyme exhibited activity in broad temperature range, from 50 to 70°C, with optimum at 55°C. It was stable at 50°C until 90 minutes, at 60°Cuntil 60 minutes and at 70°C until 30 minutes. At neutral pH this enzyme has negative charge but further purification is needed to determine its pI. The apparent Km and Vmax of this chitinase for colloidal chitin were 101.96 mg/mL and 2.72 μmol/min.mL. Addition of NaCl, KNO3 and MgSO4 decreased the activity of chitinase following mixed inhibitor mode. Additionaly, the highest degradation rate was observed within 30 minutes at 50 mg/mL substrate concentration. This enzyme should be a good candidate for applications in the recycling of chitin waste.
Keywords: Bacillus licheniformis B2, characterization, chitinase
International Conference of Natural Resources and Life Sciences 2016 73
University of Surabaya (UBAYA)
OP-D-8 The Isolation and Screening of Proteolytic Bacteria from Sidoarjo Shrimp Paste as Protease Source to Extract the Collagen Protein of Milkfish Scales
Evi Susanti*, Suharti, Naharotul Chusniyah dan Shindy Tirta Ayu Paramitha
Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Negeri Malang, Jalan Semarang 05, Malang-East Java Province, Indonesia *Corresponding author: [email protected]
Abstract This research aimed to obtain proteolytic bacteria from shrimp pasteSidoarjo which can be produce protease to be used for collagen extraction from milkfish scales. The study were consist of proteolytic bacteria isolation, selection of potential isolate, purification of potential isolates, confirmation of proteolytic index value of selected isolates, production of crude extract of protease and its ability test for collagen extraction from milkfish scales. The selective medium was used in this study skimmed milk agar (SMA) and the production medium was salt medium with peptone as a source of nitrogen. Enzyme activity was measured using casein as substrate. The protein concentration was determined by Lowry method. The ability of collagen extraction was measure based on the increaseof soluble protein resulted from hydrolysis of milkfish scales bycrude extract of protease. There were 18 isolates of proteolytic bacteria of shrimp paste Sidoarjo. Isolate of TR-10, TR-4.1.1 and TR-15.1 were produced high proteolytic with proteolytic index value of 3.00, 3.23, and 4.00. Isolate of TR- 10 and TR-15.1 produced high activity of proteases in production medium, respectively 1.07 U/mL and 0.26 U/mL, but only crude extract of protease of TR-10 that can be used to extract the collagen of milkfish scales as much as 91,1 µg protein per mg milkfish scales.
Keywords: Collagen, milkfish scales, proteolytic bacteria, shrimp paste
74 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
OP-D-9 A New Specific Primer for Detection of Pig genome by Quantitative Polymerase Chain Reaction
Mariana Wahjudi*, Stanley Evander Emeltan Tjoa, Nalista Jayanthi, Natasha Amanda, Calvin Wijaya, Ruth Chrisnasari
Laboratorium Purifikasi dan Biologi Molekuler, Fakultas Teknobiologi, Universitas Surabaya, Jl Raya Kalirungkut, Surabaya *Corresponding author:[email protected]
Abctract Detection of non-halal food is important in food certification. One of DNA- based methods for halal detection is quantitative polymerase chain reaction (q- PCR) method. Several q-PCR primers pairs for pork detection have been developed. In silico study revealed that these primers have significant similarity to the genome sequence of animals other than pigs. Therefore, it is necessary to design a specific primer that will not give any false positive to food stuffs others than pigs. This study aims to design a specific q-PCR primers for all sub- ordo suina sequences but not to other animal genomes and to evaluate the specificity and sensitivity of the primer pair in q-PCR reaction. The result showed that the MWb primers pair amplified the target sequence of pig genome with amplicon size of ~219 bp. On annealing temperature of 57 ºC and primer concentration of 0,05 µM, the limit detection of MWb primer is as low as 0,1 ng of total DNA. No amplicon is detected using cow genome as a template. The q-PCR efficiency using MWb is higher compared to three others pig primers pairs. It can be concluded that the new designed primer can be used as good primers candidate for non-halal ingredients contamination of sub-ordo Suina animals.
Keywords: Halal, MWb primers, q-PCR, Suina
International Conference of Natural Resources and Life Sciences 2016 75
University of Surabaya (UBAYA)
OP-D-10 The method growing tubular Acetobacter xylinum on the solid media as precursor of sausage casing
Salafudin1*, Y Pratama2, A H Putri1, S D Rahmatya1 and M. Mel3
1)Department of Chemical Engineering, Institut Teknologi Nasional, Jl. PKH. Mustopha No.23, Cibeunying Kaler, Bandung 40124, Indonesia 2)Department of Enviromental Engineering, Institut Teknologi Nasional Jl. PKH. Mustopha No.23, Cibeunying Kaler, Bandung 40124, Indonesia 3)Departement of Biotechnology, Faculty of Engineering, International Islamic University Malaysia, Gombak, 50728 Kuala Lumpur, Malaysia Corresponding author: [email protected]
Abstract Nata de coco is well known food in tropical countries which is made from coconut water waste using Acetobacter xylinum bacteria. The method of growing A xylinum in tubular form was studied with solid media. The tubular bio-film is used as an precursor alternative of edible sausage casing. Jelly solid media was made from coconut water waste, glucose, nutrient, and agarose powder. All ingredients was mixed and boiled, put in to tubular plastic. The variable studied ware glucose concentration (10% to 16%), duration of reaction (10 d to 16 d), and inoculation methods. The inoculation methods were scatting all surface of tubular jelly with ose needle, submersing the tubular jelly in A xylinum solution, and putting A xylinum in to warm agarose solution before the agarose solution became jelly. The experiment was done in an incubator camber which supplied with pure oxygen regularly. The measurement of parameters in the research involved COD, TPC, glucose level, and appearance of biomembrane. The results showed that the most optimum result based on the treatment method that is on the treatment of scratch while based on glucose variation of the most optimum result obtained at glucose level 10% and based on fermentation time the most optimum results showed on the 10th day of fermentation.
Keywords: Edible sausage casing, fermentation, glucose, nata de coco
76 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
POSTER PRESENTER
International Conference of Natural Resources and Life Sciences 2016 77
University of Surabaya (UBAYA)
MEDICAL & FORENSICS
78 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-A-1 Antibacterial Activity Roll On Deodorant WithPlucheaindica (L.) Leaf Extract Against Staphylococcusepidermidis in-Vitro
Oom Komala*1, Ike Yulia W 2, and Nian Rizqiyana2
1Department of Biology, Faculty of Mathematics and Natural Sciences, Pakuan University 2Department of Pharmacy, Faculty of Mathematics and Natural Sciences, Pakuan University Corresponding author: [email protected]
Abstract Plucheaindica L. is a medicinal plant that is often used to eliminate body odor. This research aims to test effectiveness Roll on deodorant as an antibacterial against Staphylococcusepidermidis and it’s stability test. Test of Antibacterial activity used dilution method. Phytochemical analysis for knowing the presence secondary metabolites on extract. Roll on Deodorant test for 4 types stored at 28-30⁰C for 8 weeks includes observation of the color, odour and homogeneity, irritation test, pH test, viscosity and specific gravity. The results showed Plucheaindica (L.) leaf extract’s Roll On Deodorant have antibacterial effect against Staphylococcusepidermidis. Pluchea indica L. ethanol extract have bioactive compounds consisting of flavonoids, saponins, tannin and alcaloid. Roll On Deodorant stored on 28-30⁰C for 8 weeks showed stable for color, odour and homogeneity. Deodorant roll on have a pH of 4.52 - 5.53, viscosity 331.8 - 340.8 cP, specific gravity 1.0107 - 1.0397 g / ml, and is not cause irritation.
Keywords: Antibacterial, deodorant roll on, Plucheaindica L
International Conference of Natural Resources and Life Sciences 2016 79
University of Surabaya (UBAYA)
PP-A-2 Formulation of Padina australis Extract Tablet as Antibacterial Escherichia coli
Tri Saptari Haryani*, Bina Lohita Sari, Triastinurmiatiningsih, Dwi Liana Hanura1
1University of Pakuan, Bogor Corresponding author: [email protected]
Abstract Padina australis (P.australis) is one of the seaweed that have a potential as antibacterial Escherichiacoli (E.coli). Triterpenoids and steroids are secondary metabolites compounds contained in seaweed, and has a variety of activities as bactericide, and hypo-allergenic. This study aims to establish tablet formulation and determine the effectiveness against of E.coli bacteria causing diarrheal disease. The formulation was prepared with these treatment: formula I (20%), formula II (25%), formula III (30%), using a negative control in the form of tablet with no active ingredient, and a positive control using amoxycillin (20 ppm). The formulas’ effectiveness was tested by calculating the inhibition zone width (IZW) –a clear zone resulting from the three formulas, negative, and positive control– using Kirby-Bauer method. The result shows that the formulation with active ingredient of 30% of P. australis extract has an inhibitory activity of 7.7 mm and effective as antibacterial Escherichia coli. Thus it can be concluded that the formulation of 30% of P. australis extract tablet is the most effective formula as antibacterial E. coli causing diarrhea disease.
Keywords: Padina australis, tablets, antibacterial, Escherichia coli.
80 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-A-3 THE POTENTIAL OF PACITAN ORANGE (Citrus sinensis) PEEL EXTRACT AS A STIMULUS OF REGENERATION CELLS IN Rattus Norvegicus BURNS
Rinza Rahmawati Samsudin*1 dan Anindita Riesti Retno Arimurti*2
1* Universitas Muhammadiyah Surabaya, Jl. Sutorejo No. 59, Surabaya 2*Universitas Muhammadiyah Surabaya, Jl. Sutorejo No. 59, Surabaya Corresponding author: [email protected]
Abstract Burns are damage to tissues that occur on the surface of the skin. Erythema is an inflammatory response that first appears in the area of inflammation during the healing process of burns. The faster the degree of erythema decreases, the faster the healing process for burns. Pacitan orange peel extract contains d- limonen active compounds, flavonoids, saponins, and tannins which can stimulate cell regeneration in burns healing. The purpose of this study was to determine the effect of extracting Pacitan orange peel for the reducing of erythema in Rattus norvegicus healing burns degree II. This research is an experimental. The research utilize 25 white rats divided into 5 different groups treatment. In K0 as a control, white rats that experienced burns degree II were only given aquadest, K1 was given burn treatment using normal saline, K2 was treated with 40% Pacitan orange peel extract, K3 was treated with burns using 60% Pacitan orange peel extract and K4 given burn treatment using 80% Pacitan orange peel extract. The treatment was carried out for 7 days. The obtained data from the research were then analyzed by Parametric Test One Way Analysis of Variance (ANOVA). Based on Homogeneity test by processing data using the Levene test, it is known that the significance value is 0.829> 0.05, so it can be concluded that the data is homogeneous. Whereas for the data processing normality test using Shapiro-Wilk test where the value of P> 0.05, it can be concluded that data data are normally distributed. The results showed that the allotment of Pacitan orange peel extract for 7 days could accelerate the reduction of erythema, significantly with a P <0.05.
Keywords: Pacitan orange peel extract (Citrus sinensis), burns, Rattus norvegicus.
International Conference of Natural Resources and Life Sciences 2016 81
University of Surabaya (UBAYA)
PP-A-4 CHARACTERIZATION AND ANTIBACTERIAL ACTIVITY TEST OF SIMBION SPONGE BACTERIA AGAINST PATHOGENIC BACTERIA
EndangSulistyarini Gultom1) and Hasruddin2) 1) Biology Lecturer at FMIPA, Medan State University, Willem Iskandar Pasar V Street, Medan Estate 2) Biology Lecturer at FMIPA, Medan State University, Willem Iskandar Pasar V Street, Medan Estate. Corresponding author: [email protected]
Abstract This study aims to characterize and explore the potential antibacterial sponge symbion bacteria from Ngge Island waters, Sibolga. The steps taken were isolation of sponge symbion bacteria, characterization of sponge symbiotic bacteria isolates, and testing of sponge symbiotic antibacterial activity against Staphylococcus aureus, Eschericia coli, and Salmonella typhi. Based on the research, 13 sponge symbiotic bacterial isolates were obtained from the third mesohyl sponge, namely 5 bacterial isolates from Haliclona sp., 4 bacterial isolates from Clathiria sp., and 4 bacterial isolates from Callyspongiasp. Morphological characterization results of sponge symbiotic bacterial colonies were obtained in a circular form on all sponge symbiotic bacterial isolates, with elevations occurring in 10 isolates, hilly in 2 isolates, convex in 1 isolate and bank in sponge symbionic isolates, 12 isolates (slippery) and 1 isolate (choppy). Based on gram staining obtained 11 gram-negative bacterial isolates, 2 gram- positive bacterial isolates, 10 bacillary isolates and 3 coccus-shaped isolates. Antibacterial potential test results for Staphylococcus aureus bacteria, Eschericia coli, and Salmonella typhii, obtained 9 potential sponge symbiotic bacterial isolates, with inhibition zones of 11 mm, 9 mm and 11 mm respectively.
Keywords: antibacterial, sponge symbion bacteria, pathogenic bacteria
82 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
AGRICULTURE
International Conference of Natural Resources and Life Sciences 2016 83
University of Surabaya (UBAYA)
PP-B-1 Genetic Variability of Soybean Genotypes for Pod Shattering Resistance using Oven Methods
Ayda Krisnawati* and M. Muchlish Adie
Indonesian Legume and Tuber Crops Research Institute Jl. Raya Kendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.: +62-341-801468, 801075, Fax: +62-341-801496 Corresponding author: [email protected]
Abstract Consumer demand for soybean is not only high yielding variety, but also must be in accordance with industrial preferences, primarily for its shattering resistance. A total of 14 soybean lines were evaluated in Nganjuk from February to May 2018. Anjasmoro variety was used for pod shattering resistant check, and Dega 1 as large seeded-size check variety. The pod shattering evaluation was using oven-dry method. The average seed yield of 16 tested lines was ranged from 3.15 – 4.06 t/ha, with the range of seed size and days to maturity were 13.55 – 20.56 g/100 seed and 78 – 84 days, respectively. All tested lines have large seed size, except SAT-Ng-A4. Five lines have early days to maturity (<80 days). The highest yielding line and large seeded size was SAT-Ng-AG-3 (4.06 t/ha), but it has medium maturity. Pod shattering evaluation which using a tiered system of temperature showed that oven temperature of 50° and 60°C were able to differentiate the soybean resistance to pod shattering. Three very resistant lines and one resistant line were obtained after exposed to 60°C. The soybean pods which allowed to dry at room temperature for six days and exposed to high temperature (80°C) showed high variability on shattering resistant. Three resistant lines (SAT-Ng-A4, SAT-Ng- 6-13, and SAT-Ng-5-5) were obtained from high temperature method, as well as from a tiered oven temperature method. Those three lines with high yield, large seed size, and medium maturity can be used as parental lines to improve soybean shattering resistance in the breeding program.
Keywords: oven method, pod shattering, soybean, yield.
84 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-B-2 Characterization of Adaptive and Productive Soybean Genotypes in Dry Land of Kalimantan, Indonesia.
M. Muchlish Adie*1, Ayda Krisnawati1, and Dotti Suryati2
1Indonesian Legume and Tuber Crops Research Institute Jl. Raya Kendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. 2Faculty of Agriculture, University of Bengkulu Corresponding author: [email protected]
Abstract Dry land is one of potential areas for soybean development. A total of 12 soybean genotypes, including two check varieties of Anjasmoro (high yield) and Demas 1 (adaptive in acidic dry land) were evaluated in two locations of dry land in Gunung Makmur Village and Kuala Tambangan Village (Takisung District, Tanah Laut Regency) from May to August 2017. The experimental design in each location was randomized complete block design with four replicates. Fertilizer consisted of 250 kg Phonska, 100 kg SP36, and 500 kg/ha dolomite, which applied entirely during sowing time. Plant and disease were controlled optimally. The location of Gunung Makmur has pH H2O of 6.1 and pH KCl of 4.2, whereas Tambangan has pH H2O of 5.8 and pH KCl of 5.0. The range of seed yield in Gunung Makmur was 1.56 – 3.18 t/ha and in Tambangan was 1.72 – 2.96 t/ha. Anjasmoro variety showed a higher productivity than Demas 1. Genotype of 19BE produced the highest yield in Gunung Makmur (3.18 t/ha), meawhile G511H/Anjs-1-3 produced the highest yield in Nambangan (2.96 t/ha). The decline in soil pH was in line with decline in seed yield. The best genotype across environments was G511H/Anjs-1-3 (2.99 t/ha), followed by 19BE (2.74 t/ha). The agronomic characters of G511H/Anjs-1-3 was medium height and a relatively low of empty pods, meanwhile 19BE has medium height and relatively high numbers of nodes per plant and branches per plant. Those genotypes could be developed in acidic dry land area of Kalimantan.
Keywords: agronomic characters, dry land, seed yield, soybean.
International Conference of Natural Resources and Life Sciences 2016 85
University of Surabaya (UBAYA)
PP-B-3 The performance of three local rice (Oryza sativa) cultivar from East Kalimantan under osmotic stress at early seedling stage
Ida Bagus Made Artadana, Ines Tiara Dewi, Johan Sukweenadhi
Department of Biology, Faculty of Biotechnology, University of Surabaya, Jalan Raya Kalirungkut, Surabaya 60292, Indonesia Corresponding author: [email protected]
Abstract Climate change made rain fall unpredictable leading to sudden drought stress during crop cultivation period. Osmotic stress induced by drought stress affect the crop growth and development especially during seedling period. This research aims to evaluate the performance of three local rice seedling under osmotic stress induced by PEG 6000. Three local rice cultivars were germinated and then divided into three groups. Three days after germination, each group were treated with either 0%, 15% or 25% PEG 6000 solution for 10 days. Osmotic stress induced by PEG significantly reduced plant growth and total chlorophyll content in the leaf. Among of the three cultivars, Mayas Putih was the most sensitive to the exposure of osmotic stress for 10 days at seedling stage. Additionally, osmotic stress induced increasing of soluble sugar in the leaf of all rice cultivars. This may indicate that soluble sugar play a role as osmoprotectant under osmotic stress in those three cultivars.
Keywords: Osmotic stress, Rice seedling, Mayas Putih, Soluble sugar
86 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-B-4 Production potensial of sweet corn (Zea mays Linn. var. saccharata) to different planting pattern and P sources
PG Adinurani1*, S Rahayu1, L S Budi1, S Pambudi1 and P Soni2
1)Faculty of Agriculture, Merdeka University of Madiun, Jl. Serayu No.79, Pandean, Madiun 63133, Indonesia 2)Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India *Corresponding author: [email protected]
Abstract Planting pattern factors that regulate population density of plant greatly determine optimal plant growth and production. The “Jajar legowo” system adopted from rice plants was applied to corn plants to obtain the effect of marginal plants. The study wanted to know sweet corn production based on planting patterns on various sources P. The study used factorial experiments which were arranged based on a randomized block design which was repeated three times with the planting pattern factor including conventional planting 70 cm x 20 cm, “Jajar Legowo” (2:1) with 20 cm x 50 cm x 100 cm and dense planting 105 cm x 12.5 cm. Source factor P consists of SP 36, mycorrhiza plus, and biofertilizer. The results showed that there was a significant interaction effect between the planting pattern and source of P on the wet weight and the dry weight of the plant, mostly in the combination treatment of the conventional system with SP 36 are 555 g and 123.51 g respectively. The weight corn cobs with and without leaf have the highest weight on the dense planting pattern which is no different significantly from conventional planting pattern.
Keywords: biofertilizer, legowo row, marginal land, mycorrhiza, SP-36
International Conference of Natural Resources and Life Sciences 2016 87
University of Surabaya (UBAYA)
PP-B-5 Local variety of “Uwi” (Dioscorea alata Linn.) plant in west region of East Java upland
Wuryantoro1, R I Fitriyani1 and P Soni2
1)Faculty of Agriculture,Merdeka University of Madiun, Jl. Serayu No.79, Pandean, Madiun 63133, Indonesia 2)Department of Agricultural and Food Engineering, Indian Institute of Technology Kharagpur, Kharagpur, West Bengal 721302, India *Corresponding author: [email protected]
Abstract Diversification efforts through empowerment of upland is a necessity for food security in the future. “Uwi” are very tolerant of various agro-climates and have potential genetic variations for different functional foods. The survey obtained 45 plant accession for testing in a controlled environment. Cluster analysis is used to trace the specific appearance of the accession based on the morphology of tubers. There was four groups of "uwi" with70 % similarity level. The first group spread in the C2, C3 and B2 agro-climates, the second group found in C2, the third group spread in C2, and C3, fourth group spreads in the C2, C3 and D3. The number of accessions analysis, 14 were found in the type C2, 32 were in the C3, two spread in agro-climate B2 and one was obtained in D3. Concluded that "uwi" plants have broad tolerance for many agro-climate so that they have the potential to be produced on a large scale to obtain alternative food and functional. Specific features of differences include the colour of tuber, the form of tubers, the taste of tubers and tuber fibre. While the similarities are in the general character of the outer skin colour and the age of the plant.
Keywords: Adaptation, food security, upland, uwi (Dioscorea alata Linn.)
88 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
ENVIRONMENT & ENERGY
International Conference of Natural Resources and Life Sciences 2016 89
University of Surabaya (UBAYA)
PP-C-1 Phytotoxicity and Developmental Effect of CASHEW Nut Shell Extract Against Nymphal Instar of Whitefly (Bemisiatabaci Genn.)
Wuye Ria Andayanie*1, Wahidin Nuriana1, Netty Ermawati2
1Agriculture Faculty, Merdeka Madiun University, Serayu No 79, Madiun, 63133, East Java, Indonesia 2State Polytechnic of Jember, 68120, East Java, Mastrip, Jember ,68101, East Java, Indonesia Corresponding author: [email protected]
Abstract Cashew nut shell (CNS), which are one of the most still is tropical biomass waste. This study was aimed to test the mortality rates of CNS extract against young nymphs (first and second instar) and old nymphs (third instar). The concentration of CNS extractswere: 0.22 %, 0.67%, 2.00 %, 6.00%. These The design of experiment was completely randomized with four treatments, and four replications. The result experiments indicated that the LD50 values of CNS extract at concentration of 0.22%, 0.67%, 2.00 %, 6.00% against younger nymphs were 0.11, 0.09, 0.24 and 0.13, respectively. Whereas the LD90 values of the same concentration of CNS extract were 0.37, 0.50, 1.59 and 0.65 %, respectively. The mortality of older nymphs required high rates concentration of CNS extract. The LD50 values of CNS extract at concentration of 0.22 %, 0.67%, 2.00%, 6.00% against older nymphs were 0.86, 1.01, 1.14, 2.11 %, respectively. However, the LD90 values of them were 3.23, 5.78, 5.33, 3.80%, respectively. These CNS extract could be used to suppress whitefly population.
Keywords: whitefly, cashew nut shell, nymphs, mortality rates
90 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-C-2 Applying species distribution models to identify the conservation area by area habitat suitability: A case study using Zanthoxyluma canthopodium DC.
Cicik Suriani*, Tri Harsono, Dina Handayani, Eko Prasetya
Departement of Biology, Universitas Negeri Medan. Jl. Willem Iskandar, Pasar V, Medan Estate, Medan 20221, North Sumatra, Indonesia Corresponding author: [email protected]
Abstract Zanthoxyluma canthopodium DC is a member of the Rutaceae family which is spread in the northern part of Sumatra, Indonesia. This plant is very closely related to Batak ethnic culture. This study aims to analyze conservation areas in accordance with habitat suitability using the species distribution modeling approach. The results of field exploration were obtained 158 points from 9 regencies in North Sumatra. The results showed that most of the Z. acanthopodium was spread on the highlands around Lake Toba. Andaliman mostly occupies an area with elevations of 1500-2000 meters with rainfall between 2000-2500 mm/year. Zanthoxyluma canthopodium occupies a narrow habitat including 6 types of land cover namely dry land agricultural land, open land, settlements, secondary dryland forests, rice fields, and shrubs. Most of these are found in dry land farming areas. Most of Andaliman were found in acrisols and a small part in orthic podzols, cambisols, and ferrasols. This research can be used as initial information in the cultivation and conservation of Z. acanthopodium.
Keywords: Zanthoxyluma canthopodium, North Sumatera, Species Distribution Modelling, Habitat Suitability Area, ArcGis.
International Conference of Natural Resources and Life Sciences 2016 91
University of Surabaya (UBAYA)
PP-C-3 An engineering of floating net aquaculture system of Tiger Grouper [Ephinephelus fuscoguttatus (Forsskal, 1775)] on suplementation Bacillus subtilis (Ehrenberg, 1835) probiotic in the diet
D Rachmawati1*, R H Setyobudi2 and Z Vincevica-Gaile3
1)Aquaculture Departement, Faculty of Fisheries and Marine Science, Diponegoro University, Jl.Prof.H.Soedarto SH, Tembalang, Semarang 50275, Indonesia 2)Postgraduate University Muhamadyah of Malang, Jl. Raya Tlogomas No. 246 Malang 65114, Indonesia 3)Department of Environmental Science, University of Latvia, Raina Blvd. 19, Riga, LV-1586, Latvia, European Union Corresponding author: dianarachmawati1964@gmail
Abstract An engineering of floating net aquaculture system of Tiger Grouper [Ephinephelus fuscoguttatus (Forsskål, 1775)] on Bacillus subtilis (Ehrenberg, 1835) probiotic suplementation in the diet is one of the solutions to overcome the deteriotion of the aquaculture environment that is caused by the accumulation of dieting waste. The purpose of the research was to study the effects of B. subtilis probiotic suplementation in the diet on protein digestibility, efficiency of diet utilization, growth, activities of digesting enzymes, and blood profile of E. fuscoguttatus. The experimental fish was raised in the floating net aquaculture. The sampled fish has an average weight of 4.24 g ± 0.023 g per fish. Diet used in the study contained 45 % protein with the suplementation of various amounts of B. subtilis probiotic. The amounts were 0 % per kg diet (A treatment), 5 % per kg diet (B treatment), 10 % per kg diet (C treatment), and 15 % per kg diet (D treatment). The results show that B. subtilis probiotic suplementation in the diet significantly affected on protein digestibility (ADCP), efficiency of diet utilization (EFU), feed conversion ratio (FCR), protein efficiency ratio (PER), relative growth rate (RGR), survival rate (SR), and activities of digesting enzymes; however, it did not significantly influence blood profile of E. fuscoguttatus. C treatment with the B. subtilis probiotic suplementation dose of 10 % per kg diet was the best dose. The optimum doses of B. subtilis probiotic addition for ADCP, EFU, FCR, PER, RGR and SR were (9.42, 9.42, 10.00, 9.63, 9.63, 9.48) % per kg diet respectively.
Keywords: Blood profile, diet utilization, digesting enzymes, growth, protein digestibility
92 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-C-4 Performance UNEJ #1 stove low energy using bio-pellet from coffee husk
S S Harsono1, M B N Prayogo1, Razhika1, R H Setyobudi2 and P Soni3
1)Departement of Agricultural Engineering, Faculty of Agricultural Technology University of Jember, Jl. Kalimantan No. 27, Jember 68121, Indonesia 2)Waste Laboratory, University Muhamadyah of Malang, Jl. Raya Tlogomas No. 246 Malang 65114, Indonesia 3)Departement of Food, Agriculture and Bioresources, School of Environment, Resources and Development, Asian Institute of Technology, PO Box 4, Klong Luang Pathumthani 12120, Thailand Corresponding author: [email protected]
Abstract The level of fuel consumption especially of fossil fuels will increase along with increasing human population and the rising rate of the industry in many countries. There will be causing a fuel crisis especially fuel oil and gas if this happens. Bio-pellet from coffee husk is a renewable energy substitute for oil which is environmentally friendly. Performance test of coffee husk biopellet as a source for UNEJ#1 biomass stove was carriout.The variable include heat, thermal efficiency, and emissions, with UB-03 stove as control.. The result shows that heat on 40 holes of UNEJ#1 stove is 316 880 kJ, 20 holes of UNEJ#1 stove is 310 601 kJ, 10 holes UNEJ#1 stove is 308 229 kJ, and UB-03 stove is 306 833 kJ. Thermal efficiency of UB-03 stove is 16.47 %, 10 holes of UNEJ#1 stove is 16.39 %, 20 holes of UNEJ#1 stove is 15.96 %, and 40 holes of UNEJ#1 stove is 15.38 %. Emissions result of UB-03 stove is 333 mg kg–1, 10 holes UNEJ#1stove is 298 mg kg–1, 20 holes of UNEJ#1 stove is 289 mg kg– 1, and 40 holes of UNEJ#1 stove is 273 mg kg–1.
Keywords: Bio refinery, emission, renewable energy, coffee solid waste, thermal efficiency
International Conference of Natural Resources and Life Sciences 2016 93
University of Surabaya (UBAYA)
PP-C-5 Fish diversity in Cimanuk river before and after rainy season in Jatigede reservoir, West Java, Indonesia
T Herawati1*, AYustiati1, I Adiwiguna2,IRostini1A Sahidin1 and H Juahir 3
1)Aquaculture Departement, Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung Sumedang Km. 21, Jatinangor45363, Indonesia 2)Alumnus Faculty of Fisheries and Marine Science, Universitas Padjadjaran, Jl.Raya Bandung Sumedang Km. 21, Jatinangor 45363, Indonesia. 3)East Coast Environmental Research Institut -Sultan Zainal Abidin University, Gong Badak, 21300 Kuala Terengganu, Malaysia *Corresponding author: [email protected]
Abstract. The purpose of the research was to map the diversity of fish living in the Cimanuk River before and after the Jatigede Reservoir. Research using field observation method, and census sampling technique. Research is done in the rainy season in November 2017 until January 2018, fish taken from four stations. Parameters measured by the diversity index, dominance index, equitability index, and water quality. Data analysis used a comparative descriptive method by mapping the species and comparing the diversity of fish before and after the Jatigede Dam. The results of the research indicate that the identified fishes are six families consisting of 14 species, the diversity of Cimanuk River fish before Jatigede Reservoir (station I and II), including moderate diversity 1.735≤H'≤1.909, low species dominance 0.185≤C≤0.194, high equitability 0.61≤E≤0.789, stable fish community structure. Diversity of fish after Jatigede Reservoir (station III and IV), including low with 0.759≤H'≤0.855, moderate species dominance 0,55≤C≤0.57, low equitability 0.296≤E≤ 0.317, distressed fish community structure. Water quality in general meets the requirements of water quality standards for fisheries, except for light penetration, nitrite at station I, IV and ammonia at station III that has exceeded environmental quality standards, but can still be tolerated by several types of fish.
94 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
Keywords: Diversity, environmental, equitability, fish map, water quality
International Conference of Natural Resources and Life Sciences 2016 95
University of Surabaya (UBAYA)
PP-C-6 The Fe non-heme prospectin coffee flour made from coffee solid waste: Mini review
R H Setyobudi1,*, L Zalizar2, S K Wahono3,4, S S Harsono5, P G Adinurani6, J Burlakovs7, Y A Nugroho8 and T Liwang8
1)Waste Laboratory of University Muhamadiyah of Malang, Jl. Raya Tlogomas No. 246, Malang 65114, Indonesia 2)Faculty of Agricultural and Animal Science, University Muhamadiyah of Malang, Jl. Raya Tlogomas No. 246, Malang65114, Indonesia 3)Future Industry Institute and School of Engineering, University of South Australia, Mawson Lakes SA 5095, Adelaide, Australia. 4)Technical Implementation Unit for Development of Chemical Engineering Processes, Indonesian Institute of Sciences, 55861, Yogyakarta, Indonesia 5)Faculty of Agricultural Technology University of Jember, Departement of Agricultural Engineering, Jl. Kalimantan No. 27, Jember 68121, Indonesia 6)Faculty of Agrotechnology, Merdeka University of Madiun, Jl. Serayu No. 79 Madiun 63131, Indonesia 7)Faculty of Health and Life Sciences, Departemen of Biology and Environmental Science, Linnaeus University, 391 82 Kalmar/ 351 95 Växjö, Sweden 8)Plant Production and Biotechnology Division, PT Sinarmas Agroresources and Technology Tbk., Sinar Mas Land Plaza, 2nd Tower 10th Fl. Jl. M.H. Thmrin No. 51, Jakarta 10350, Indonesia
Abstract. Coffee flour (CF) from coffee pulp or husk,solid waste of coffee processing have launched in Canada since 2014. This product is claimed as certified of gluten-free, vegan, kosher, paleo, and non-GMO.CF is stated to contain three times Fe content than fresh spinach (Amaranthus spp.); five times fiber content than wheat flour; 84% fat content lower and 42% fiber content higher than coconut flour; 38 % anti-oxidant content higher than pomegranate (Punica granatum); three times protein content of kale (Brassica oleracea var. sabellica); and two times potassium content of banana (Musa paradisiaca). Several receipts of cookies, donuts, and cakes using CF has been introduced as wheat flour substitution. However, the scientific publication of CF effect for health
96 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
does not appear until August 2018 yet. The mini-review research was conducted to investigate the Fe content in CF. This Fe non-heme prospect is allegedly unable to be absorbed optimally by the organism. Coffee pulp and husk contain an inhibitor, such as tannin, caffeine, phenol, dan calcium which detain Fe absorption. On the other hand, the promoter/enhancer of Fe absorption such as vitamin C and amino acid was released in CF processing. Several types of research have to be conducted to tackle this problem.
Keywords: Biorefinery, Fe inhibitor, Fe promoter, hemoglobin, zero waste
International Conference of Natural Resources and Life Sciences 2016 97
University of Surabaya (UBAYA)
PP-C-7 Does the utilization of waste as the green energy alternative economically feasible?
N Syaifudin1,2*, Nurkholis3, R Handika4 and RH Setyobudi5
1UWA Business School, Crawley Campus, The University of Western Australia, 35 Stirling Highway, 6009 Perth, Australia 2Fiscal Policy Agency, Ministry of Finance of Republic of Indonesia, Notohamiprodjo Building, 1st Floor, Jl. Wahidin No 1, Senen, Jakarta, 10710, Indonesia 3Faculty of Economics, University of Indonesia, Economics Department Building, 1st Floor, Depok, West Java, 16424, Indonesia 4College of Business Administration, Abu Dhabi University, Abu Dhabi Campus, P.O Box 59911, Abu Dhabi, Uni Arab Emirates 5Waste Laboratory University Muhamadiyah of Malang, Jl. Raya Tlogomas No. 246 Malang, 65114, Indonesia Corresponding author: [email protected]
Abstract The high demand for fossil fuel energy is a problem and obstacle for implementing the Green Economy. The higher the fossil price the higher the cost burden on production activities, whether for industry or households. On other hand, the high international price of energy is escalating the significant burden of the government budget subsidy for fuel and energy prices. Optimization of waste to be transformed into energy (WtE) can be one alternative solution to the energy crisis and also to help implement the Green Economy policy. Optimization of waste treatment has great potential for providing net benefits. This study particularly will discuss the potential of the utilization of waste as the alternative green energy. Further, the economic feasibility study will be conducted to assess the net benefit of the implementation of the policy to the economy.
Keywords: environmental friendly, subsidized fuel, green economy, renewable energy, waste to energy
98 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
FOOD & NUTRITION
International Conference of Natural Resources and Life Sciences 2016 99
University of Surabaya (UBAYA)
PP-D-1 Preparation and Characterization of Butterfly Pea Color Extract Encapsulated with Various Coating Agents
Hana Susanti Maleta1, Renny Indrawati*1,2, Rosita Dwi Chandra1
1Ma Chung Research Center of Photosynthetic Pigments, Renny Indrawati, Universitas Ma Chung, Villa Puncak Tidar N1, Malang, 0341-550171, 081334678375 2Chemistry Study Program, Universitas Ma Chung, Villa Puncak Tidar N1, Malang, 0341-550171 Corresponding author: [email protected]
Abstract Nowadays, the use of natural pigments in food products has gained much interest in the society, particularly due to the rise in the number of reported side effects of synthetic colorants. Anthocyanin, a pigment responsible for red to purplish blue color, has a potential use as natural food colorants because of its great solubility in water. Among many kinds of its plants sources, the butterfly pea (Clitoria ternatea L) color extract exhibits vivid blue color at wide range of various pH environments. Hence, it is often used as the source of natural blue colorant. However, the primary obstacle of the use of natural pigments is the poor stability during food processing. The present study is aimed to prepare and do the characterization of the color extract of butterfly pea which is encapsulated with various coating agents. The encapsulating materials were maltodextrin (MDX), mixture of maltodextrin and sodium alginate (MDX- NaAlg), and polyvinyl-pyrrolidone (PVP). The color value, anthocyanin content, antioxidant activity, and encapsulation efficiency weredetermined prior to stability evaluation. The results showed that the blue anthocyanin coated by 15% of PVP performedhighest antioxidant activity, whereasthe highest encapsulation efficiency was presented by combination of MDX-NaAlg. Furthermore, the SEM profiles of each lyophilized powder confirmed its microstructures.
Keywords: anthocyanin, butterfly pea, antioxidant, encapsulation, lyophilization.
100 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-D-2 Photodynamic Inactivation of Escherichia coli by the Crude Pigments Extract of Various Tea Dregs
Renny Indrawati*1,2, Jovine Marcella Kurniawan1, Ivanna Gunawan1, Cledwyn Davian Irsan1, Heriyanto1,2, and Leenawaty Limantara3
1Department of Chemistry, Faculty of Science and Technology, Universitas Ma Chung, Jl. Villa Puncak Tidar N-1, Malang 65151, Indonesia, 081334678375 2Ma Chung Research Center for Photosynthetic Pigments (MRCPP), Universitas Ma Chung, Jl. Villa Puncak Tidar N-1, Malang 65151, Indonesia 3Center of Urban Studies, Universitas Pembangunan Jaya, Jl. Cendrawasih, Sawah Baru, Ciputat, Tangerang Selatan 15413, Indonesia *Corresponding author: [email protected]
Abstract Inactivation of pathogenic microorganism is one of the critical points that must be ensured during food processing. However, common procedures applied by food manufacturers are either thermal processes or chemical treatments, which may impact on nutrient loss or unwanted chemical residue. Photodynamicmethod for microbial inactivation proposes a non-thermal food processing with a relative short time, which involves three main components, i.e. sensitizer agent, light and oxygen, to generate the reactive oxygen species that induces the death of microbial cell. In fact, the tea dregs contained significant amount of chlorophylls and its derivatives that possess photodynamic activity as sensitizers. The aims of the present study were: (i) to determine the composition of chlorophylls and its derivatives in the tea dregs of black, green, oolong tea, and (ii) to evaluate the in vitro photodynamic activity of its crude pigment extracts against Escherichia coli. The tea dregs were extracted using acetone, and the pigments fractions were identified by means of thin layer and high performance liquid chromatography. Purified chlorophyll, pheophytin, and pheophorbide were used as references during the evaluation of photodynamic activity. The results showed that the dregs of black and oolong tea containedhigher amount of pheophorbide a and pheophytin a than that of green tea. The inactivation activity of the crude pigments extracts of tea dregs against E. coli, compared to the references, was followed the order: pheophorbide > pheophytin >black tea> oolong tea >green tea> chlorophyll. Therefore, the tea dregs could be used as rich and inexpensive source of sensitizing agents for inactivation of pathogenic microorganism through photodynamic method.
Keywords: chlorophyll derivatives, microbial inactivation, photodynamic, sensitizer, tea dregs International Conference of Natural Resources and Life Sciences 2016 101
University of Surabaya (UBAYA)
PP-D-3 The Function of Ethanolic Extract Plectranthus ambioinicus Lour Spreng as Preventive and Curative Against The Effects of Rhodamine B in Rats
Melva Silitonga*, Pasar Maulim Silitonga, Martina Restuaty
Universitas Negeri Medan, Medan North Sumatera Indonesia Corresponding author: [email protected]
Abstract Rhodamin B is a flavor enhancer, is used in modern nutrition to improve food palatability. The objectives of the current study were to investigate the effect of ethanolic extract of Plectranthus amboinicus L. Spreng (EEP) as a preventive and curative to the toxic effects of rhodamine B. Adult male rats were divided into eight groups: the negative control (CMC), the positive control (980 mg Rhodamin B)/kg bw, preventive 350, 700 and 1050 mg EEP / kg bw and curative 350, 700 and 1050 mg EEP / kg bw. EEP and Rhodamin-B was given by oral gavages. On preventive treatment was done by giving EEP from the 1st day to 42nd then rhodamin B was given from 21st to 42nd day. Whereas in curative treatment was done by first inducting rhodamin B from the first day to the 21st, then EEP is gave from the 22nd day until the 42nd. Parameters were observed namely body weight, organ ratio, ALT and AST. The data were analyzed with ANOVA and followed by LSD. The results showed that in preventive and curative treatment EEP dose 700 mg/kg bw increased body weight, 1050 mg/kg bw increased ratio liver weight to body weight significantly (p<0.05). On preventive treatment EEP decreased ALT and AST significantly, whereas in the curative treatment, not only ALT but also AST increased significantly in the treatment of Rhodamine-B and all EEP doses have not been able to reduce the levels of ALT and AST.
Keywords: Plectranthus amboinicus L. Spreng, body weight, ratio liver weight, ALT, AST
102 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-D-4 Potency of Vegetable Oil to Increase Microflora Health Using In Vitro and In Vivo Evaluation
Nurhayati Nurhayati*, Vika Nurluthfiyani Ni’maturrakhmat, and Yuli Witono
Department of Agricultural Product Technology Faculty of Agricultural Technology, University of Jember. Jl. Kalimantan No. 37 FTP UNEJ Jember 68121 Corresponding author: [email protected]
Abstract Vegetable oil is a food ingredient that is often consumed along with other foods. Some types of vegetable oils include coconut oil, palm oil, olive oil, corn oil, soybean oil, sesame oil, etc. These study evaluated the potency of vegetable oil i.e coconut oil, palm oil, olive oil, corn oil, soybean oil to improve the microflora human health. In vitro evaluation was based on the ability of vegetable oil as growing medium for microflora models. In vivo evaluation was based on the microflora modulation of rat faecal consumed the vegetable oil. Result of in vitro evaluation showed that coconut oil was better to increase the probiotic population of microflora up to 2.7%. Olive oil decreased the population of pathogenic bacteria up to 51.3%. Result of in vivo evaluation showed that olive oil was more capable to increase the probiotic population up to 16.5%, while coconut oil was more capable to reduce the pathogen population up to 5,1%. Corn oil can promote the Wistar rat to eat more the feed. The water consumption increase by consuming olive oil, but coconut oil gave the opposite affect.
Keywords: vegetable oil, coconut oil, Wistar rat, probiotic, microflora
International Conference of Natural Resources and Life Sciences 2016 103
University of Surabaya (UBAYA)
PP-D-5 Quality and Consumer Acceptance Improvement on Prime Local Soy Milk (Glycine max (L) Merrill) Varieties
Elfi Anis Saati*1, Sri Wahyuni2, Sri Winarsih1, Khoiriyah3
1Instructor of Food Science and Technology Program, Agriculture and Animal Husbandry Faculty, Muhammadiyah University of Malang 2Alumnus of Food Science and Technology Program, Agriculture and Animal Husbandry Faculty, Muhammadiyah University of Malang *Corresponding author: [email protected]
Abstract Soy milk is a healthy drink that has a lot of consumer. One of soy milk problem is its off flavor that needs technique to improve the taste. This study aimed to determine the effect of local soybean varieties, soaking method, and natural essence use on soy milk quality. Randomized Complete Block Design (RCBD) factorial, 2 factors were used. The first factor was soybean varieties that consisted of Argomulyo, Bromo, Wilis, and Anjasmoro. The second factor was soaking method that comprised of without soaking, soaked in water for 4 hours, soaked in water for 8 hours, soaked in NaHCO3 0.5% for 15 minutes, soaked in Ca(OH)2 0.5% for 15 minutes, soaked in water and boiled in Ca(OH)2 0.5% for 15 minutes. Natural essence (3%) added in the best result, repeated twice. The best treatment of this was Agromulyo soybean soaked in NaHCO3 0.5% for 15 minutes in which soy milk has moisture content 84.54%, protein content 2.67%, total dissolved solids 14.00 0Brix, pH 7.10, no off flavor (2.20 points), appearance 3.53 points (interesting enough), preference 3.21 points (like enough), and high in antioxidant (90.74%).
Keywords: Soy milk, prime soy bean varieties, soaking method, natural essence
104 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-D-6 The Utilization of Telang Flower as Healthy-Natural Food Coloring on Dawet Drink
Elfi Anis Saati*1, Rachmi Diah Mulandari2, Moch. Wachid1, Sri Winarsih1
1Lecturer of Food Science and Technology Department, Agricultural and Animal Husbandry Faculty, University of Muhammadiyah Malang 2Alumnus of Food Science and Technology Department, Agricultural and Animal Husbandry Faculty, University of Muhammadiyah Malang *Corresponding author: [email protected]
Abstract The use of coloring in food industry increased. One of natural food coloring source is flower. Natural blue pigment is hard to get. Anthocyanin is a flavonoid that dissolved in water, its pigment range are red to blue and easily found in plants. Anthocyanin is not stable in neutral or base condition, so it needs to be extracted in acid condition. Telang flower or Clitoria ternatae L. is one of blue anthocyanin pigment source. Telang flower anthocyanin extract can be alternative as synthetic food coloring substitute. Simple randomized block design was used in this study. This study consisted of 2 steps. The first step was anthocyanin pigment extraction from telang flower using citric acid and tartaric acid with concentration variation 0.25%, 0.50%, and 0.75%. Extract total anthocyanin, total sugar, total dissolved solids, pH, color intensity, and yield were analyzed for the first step. The second step was telang flower anthocyanin extract application on Dawet and compared to synthetic food coloring and textile coloring. The best treatment was telang flower anthocyanin extract and 0.75% tartaric acid (P6) with pH 3.17, total dissolved solids 2.53oBrix, brightness (L) 32.33, redness (a+) 4.17, bluish (b-) 2.27, total anthocyanin 56.82 mg/ml, yield 39.13%, and anthocyanin decreased level when applied to dawet 70.78%, but still considered more preferred than dawet with synthetic food coloring.
Keywords: Anthocyanin, Telang flower, extraction, citric acid, tartaric acid
International Conference of Natural Resources and Life Sciences 2016 105
University of Surabaya (UBAYA)
PP-D-7 Artificial Rice as an Alternative Functional Food to SupportFood Diversification Program
Erica Valencia and Maria Goretti Marianti Purwanto*
Faculty of Biotechnology, University of Surabaya, Indonesia *Corresponding author: [email protected]
Abstract Functional food is a food that has a beneficial effect to the body beyond its adequate nutritional effects that can help improve someone’s health and/or decrease the risk of disease. Awareness of the importance of health that began to grow in the community encourage the development of functional food. One of the functional food that has been developed in Indonesia is in the form of artificial rice. Artificial rice is an alternative paddy rice substitute that has a minimally equal value to paddy rice. Artificial rice is made to reduce people’s dependence on paddy rice and to support the food diversification program so that the food security in Indonesia can be achieved. Artificial rice is made from a non-paddy rice flour with a certain composition, so it can be used as a vehichle to make a functional food and can be fortified with ingredients that contain functional active compounds. Until now, many Indonesian researchers have developed artificial rice products by utilizing local resources, such as corn, sorghum, spices, tubers, and others, to obtain an artificial rice with improved nutritional and functional characters that have some health benefits such as antidiabetic, antioxidant, antihypertensive, and anticancer. From mostartificial rice that have been developed, it can be seen that analog rice can be used as a functional food for rice substitute with equal or even more (better) nutritional value thanpaddy rice. However, further research is still needed to improve the sensory quality of artificial rice so that it can be accepted easily by the community.
Keywords: Artificial rice, rice analogue, functional food, food diversification
106 International Conference of Natural Resources and Life Sciences 2018
University of Surabaya (UBAYA)
PP-D-8 Critical Aspects to Produce Low-Cost Protein Molecular Weight Marker
Leonardo Tejo Gunawan P.H. and Maria Goretti Marianti Purwanto*
Faculty of Biotechnology, University of Surabaya, Indonesia *Corresponding author: [email protected]
Abstract Proteomic studies usually begin with characterizing protein profile, like molecular weight of protein which can be done by SDS-PAGE technique followed by Western Blot. These methods need a standard called molecular weight marker (MWM). MWM is used to compare the MW of protein in standard with the protein in the samples based on their rate flow. MWM is commercially available, however, for some researchers, the price are quite expensive coupled with long time waiting period. In this review, the important basic aspects of either recombinant or native MWM production will be discussed, including the type and effect of dyes can be used for preparing prestained MWM. Moreover, component like buffer and polyols can also affect the quality dan stability, also other adjuvant may be needed to increase the robustness of MWM to lower the risk of protease contamination that can breakdown the protein inside the MWM. Understanding those critical aspects will help researchers to produce MWM and reduce dependence on commercial products.
Keywords: Molecular Weight Marker (MWM), protein dyes, formulation, stability.
International Conference of Natural Resources and Life Sciences 2016 107
University of Surabaya (UBAYA)
PP-D-9 Potency healthy Moringa oleifera (Lamk) leaf flour as home- agroindustry in rural area of Eastren East Java - Indonesia
S S Harsono1, I Syafii2, Mustaqim2, R H Setyobudi3 and J Burlakovs4
1)Departement of Agricultural Engineering, Faculty of Agricultural Technology University of Jember, Jl. Kalimantan No. 27, Jember 68121, Indonesia 2)Farmer Jember Association, Kesilir , Jember 67121, Indonesia 3)Waste Laboratory, University Muhamadyah of Malang, Jl. Raya Tlogomas No. 246 Malang 65114, Indonesia 4)Faculty of Health and Life Sciences, Departemen of Biology and Environmental Science, Linnaeus University, 391 82 Kalmar/ 351 95 Växjö, Sweden *Corresponding author:[email protected]
Abstract Moringa oleifera have been widely known in Indonesia, especially in rural areas and moorlands of Eastern East Java Province of Indonesia. Moringa leaf flour, utilizes a group of women farmers in the surrounding area to process food products made from Moringa leaf flour, namely crackers and Moringa sticks, so that later there will be a partnership between a a home-agroindustry with farmer of Moringa self. Moringa leaf flour processed products is one new products so that there is a need for a development strategy. The study was conducted on Kesilir Agroindustry of Jember – Indonesia. The method used is descriptive and analytical methods. The sampling method is purposive sampling. This study aims to determined the business collaboration between home-agroindustry made from Moringa leaf flour and farmer of Jember. Product revenue in one production process in agro-industry made from Moringa leaf flour, value-added products made from Moringa leaf flour, and prospects for agro-industry development made from Moringa leaf flour as healthy-food materials.
Keywords: Fungsional food, healthy food, Kesilir agroindustry, Moringa crackers, Moringa sticks
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University of Surabaya (UBAYA)
PP-D-10 Quality of milk pasteurization of ginger in cold strorage
K Khotimah1, H Chairunnisa2, B Etty1, N H Nia1 and Z Vincevica-Gaile3
1)Faculty of Agricultural and Animal Science, University Muhamadiyah of Malang, Jl. Raya Tlogomas No. 246, Malang 65114, Indonesia 2)Faculty of Animal Science, Universitas Padjadjaran, Jl.Raya Bandung Sumedang Km. 21, Jatinangor 45363, Indonesia 3)Department of Environmental Science, University of Latvia, Raina Blvd. 19, Riga, LV-1586, Latvia, European Union *Corresponding author: [email protected]
Abstract Utilization of natural materials, such as ginger as a flavor and preservative is one alternative in maintaining the quality of pasteurized milk. Ginger contains antibacterial compounds that can prevent microbial growth so as to provide lasting effect on pasteurized milk.. The ethanol extract and chloroform ginger appeared to inhibit the growth of bacteria Klebsiella neumonia, Salmonella thyphimurium, Bacillus cereus, Enterococcus fecalis and Staphylococcus aureus. The purpose of this research is to know the physicochemical quality which includes the specific gravity, viscosity and protein content, and the microbiology quality and the existence of Escherichia coli as one of the requirements of quality of pasteurized milk worth of consumption. The material used in this research is pasteurized milk with ginger flavor and kept at cold temperature for 4 wk for 30 d. The method used in this study is a survey with gradual observation starting before being stored, 6 d, 12 d, 18 d, 24 d, and 30 d. Data analysis is quantitative. The results of this study indicate that the longer the stroge showed a decrease in viscosity and protein content, but up to day 30th there was no E coli on ginger pasteurized milk kept at 4 °C.
Keywords: anti bacterial, Escherichia coli, flavor, natural material, preservative
International Conference of Natural Resources and Life Sciences 2016 109
University of Surabaya (UBAYA)
MISCELANEOUS
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About Paper Publication
Only qualified and selected papers will be published either at Scopus Indexed International Proceeding or Scopus Indexed International Journals (Q3 or Q4 Journal).
The participant who willing to publish their papers along with us, please submit the full article to [email protected] before September 3rd 2018.
The article format can be found at http://event.ubaya.ac.id/nrls/call-for-paper/
Please sign the “Author Consent Form” which attached on the goodie bag or request it at our secretariat desk.
The publication fee will cost IDR 1.750.000 for the Proceeding and IDR 2.500.000 for the Journal. The payment can be done by transfer the right nominal to BCA Account No. 5120446772 (Marianti Purwanto).
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